##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936002.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572016
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.087864675113984	33.0	31.0	34.0	30.0	34.0
2	32.210310550753825	33.0	31.0	34.0	30.0	34.0
3	32.15019509943778	33.0	31.0	34.0	30.0	34.0
4	35.78305676764286	37.0	35.0	37.0	35.0	37.0
5	31.999788467455456	37.0	35.0	37.0	0.0	37.0
6	33.82627933484378	37.0	35.0	37.0	17.0	37.0
7	35.495381248076974	37.0	35.0	37.0	32.0	37.0
8	35.72118961707365	37.0	35.0	37.0	35.0	37.0
9	37.66478560040279	39.0	37.0	39.0	35.0	39.0
10	37.3647957399793	39.0	37.0	39.0	34.0	39.0
11	37.314206245979136	39.0	37.0	39.0	34.0	39.0
12	37.19352255880954	39.0	37.0	39.0	34.0	39.0
13	37.091528908282285	39.0	37.0	39.0	33.0	39.0
14	38.31892639366731	40.0	38.0	41.0	34.0	41.0
15	38.450251391569466	40.0	38.0	41.0	34.0	41.0
16	38.41071228776818	40.0	37.0	41.0	34.0	41.0
17	38.370082305390056	40.0	37.0	41.0	34.0	41.0
18	38.25694386171016	40.0	37.0	41.0	34.0	41.0
19	38.09523509831893	40.0	37.0	41.0	34.0	41.0
20	38.092639017090434	40.0	36.0	41.0	34.0	41.0
21	38.040988363961844	40.0	36.0	41.0	34.0	41.0
22	38.1095738580739	40.0	36.0	41.0	34.0	41.0
23	38.09828571228777	40.0	36.0	41.0	34.0	41.0
24	38.071823165785574	40.0	36.0	41.0	34.0	41.0
25	37.844675673407735	40.0	35.0	41.0	34.0	41.0
26	37.84504279600571	40.0	35.0	41.0	34.0	41.0
27	37.811134653576126	40.0	35.0	41.0	34.0	41.0
28	37.74892135884311	40.0	36.0	41.0	33.0	41.0
29	37.70990671589606	40.0	36.0	41.0	33.0	41.0
30	37.540899205616626	40.0	35.0	41.0	33.0	41.0
31	37.51446463035999	40.0	35.0	41.0	33.0	41.0
32	37.326955889345754	40.0	35.0	41.0	33.0	41.0
33	37.17728175435652	40.0	35.0	41.0	33.0	41.0
34	37.05674491622612	40.0	35.0	41.0	32.0	41.0
35	36.98836221364437	40.0	35.0	41.0	32.0	41.0
36	36.8522418953316	40.0	35.0	41.0	31.0	41.0
37	36.7854553019496	40.0	35.0	41.0	31.0	41.0
38	36.69949441973651	39.0	35.0	41.0	31.0	41.0
39	36.599068907163435	39.0	35.0	41.0	31.0	41.0
40	36.520155030628516	39.0	35.0	41.0	31.0	41.0
41	36.41244126038433	39.0	35.0	41.0	30.0	41.0
42	36.37225357332662	39.0	35.0	41.0	30.0	41.0
43	36.284287502447484	39.0	35.0	41.0	30.0	41.0
44	36.25614842941456	39.0	35.0	41.0	30.0	41.0
45	36.232136863304525	39.0	35.0	41.0	30.0	41.0
46	36.10204784481553	39.0	35.0	41.0	30.0	41.0
47	36.0147373500042	39.0	35.0	41.0	30.0	41.0
48	35.971048012643	38.0	35.0	41.0	30.0	41.0
49	35.93695281250874	38.0	35.0	41.0	30.0	41.0
50	35.8244349808397	38.0	35.0	40.0	29.0	41.0
51	34.88471476322341	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	17.0
9	20.0
10	19.0
11	15.0
12	8.0
13	15.0
14	22.0
15	25.0
16	44.0
17	76.0
18	168.0
19	318.0
20	587.0
21	1091.0
22	1759.0
23	2448.0
24	3233.0
25	4408.0
26	5574.0
27	6219.0
28	6426.0
29	7047.0
30	8388.0
31	10277.0
32	13765.0
33	20631.0
34	35606.0
35	49628.0
36	43407.0
37	65425.0
38	106370.0
39	178851.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68861010880815	18.367143576403457	22.803033481580933	14.141212833207462
2	33.40378590808648	25.058914435959835	23.755104752314622	17.78219490363906
3	30.751587368185508	25.630227126513944	27.27301334228413	16.345172163016418
4	27.551676876171292	24.489349948253196	29.79182400492294	18.167149170652568
5	23.33151520237196	35.61736035355654	23.679932029873292	17.371192414198205
6	24.675533551509048	34.65864591200246	26.077592235182234	14.588228301306257
7	76.140702357976	6.077802019523929	13.011524153170539	4.769971469329529
8	76.1917498811222	7.801704847416856	11.228357248748287	4.778188022712651
9	70.45362367486224	7.693840731727784	12.983727727895722	8.86880786551425
10	40.03122290285586	25.6489328969819	19.538963945064474	14.780880255097758
11	32.25976196470029	23.91524013314313	25.44526726525132	18.37973063690526
12	28.463539481413108	21.187868870800816	29.73133618640038	20.617255461385696
13	25.389674414701684	23.714896086822744	31.131996307795585	19.76343319067998
14	20.28160051467092	28.01215350619563	30.276775474811895	21.429470504321557
15	19.190372297278397	24.638996111996867	35.692008615143635	20.478622975581104
16	21.882779502671255	23.232392100920254	32.7892926071998	22.095535789208693
17	21.242762440211465	24.30771167240077	30.067865234538893	24.381660652848872
18	22.97051131436883	24.256139688400324	30.645121814774413	22.128227182456435
19	23.85982210287824	25.954343934435396	28.094493860311598	22.091340102374758
20	26.61394086878689	24.807697686777992	29.25967105815222	19.318690386282903
21	23.84828396408492	27.408499062963276	28.927687337417137	19.81552963553467
22	21.758657100500685	24.348444798746886	29.849864339459035	24.04303376129339
23	22.05445302229308	26.816382758524238	29.634310928365643	21.49485329081704
24	22.78030684456379	23.93499482531957	30.698791642191825	22.585906687924815
25	21.295732986489888	26.740860395513412	28.80461385695505	23.15879276104165
26	20.61952812508741	26.318319767279235	30.184645184750075	22.877506922883274
27	22.037145814103102	26.6291502335599	28.66475762915723	22.66894632317977
28	18.924470644177784	26.807991384856365	31.35594109255685	22.911596878408997
29	21.191190456211014	25.393170820396634	30.834102542586223	22.58153618080613
30	21.44660289222679	27.279831333389275	29.874513999608403	21.399051774775533
31	23.45843472909849	25.856969035831167	26.673904226455203	24.010692008615145
32	24.205791446393107	27.674575536348634	27.068998069984058	21.0506349472742
33	23.094808536824143	27.79834829794971	26.57862717126794	22.528215993958213
34	21.410415093284104	26.05189365332438	28.539935945847667	23.997755307543844
35	22.026132136164023	26.877744678470535	28.53276831417303	22.563354871192416
36	21.706036194791754	28.771572823137813	27.970196637856283	21.552194344214147
37	21.360416491846383	29.28781712399653	27.790831025705575	21.56093535845151
38	20.863577242594612	29.05320830186568	25.905044614136667	24.178169841403037
39	22.130150205588656	27.108507454336944	27.83278789404492	22.92855444602948
40	23.453364940840814	25.340724734972447	28.317040082794886	22.88887024139185
41	19.87724119605046	25.49911191295348	29.1161436043747	25.507503286621354
42	22.116339403093622	25.34824200721658	28.73311236049341	23.802306229196386
43	21.909701826522333	25.930743195994516	28.61755615227546	23.541998825207685
44	20.90868087605941	25.82078123688848	28.394660289222678	24.875877597829433
45	20.971266537998936	25.77829990769489	27.500978993594583	25.749454560711587
46	22.5448239210092	26.80641800229364	27.394338619898743	23.254419456798413
47	20.25188106626388	26.380555788649268	30.186743028167047	23.180820116919808
48	20.97249027999217	25.546488210119993	29.259321417582722	24.22170009230511
49	21.298530111045842	24.95507118681995	30.425722357416575	23.32067634471763
50	19.89384912310145	25.43635143072921	29.652492237979356	25.017307208189983
51	19.554348130122236	24.806823585354255	28.233301166400942	27.40552711812257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	443.0
1	818.5
2	1194.0
3	4206.0
4	7218.0
5	4715.0
6	2212.0
7	2160.5
8	2109.0
9	2148.0
10	2187.0
11	2283.0
12	2379.0
13	2231.0
14	2083.0
15	2017.5
16	1952.0
17	2013.5
18	2075.0
19	2101.0
20	2127.0
21	2271.0
22	2415.0
23	2549.0
24	2683.0
25	3219.0
26	4169.5
27	4584.0
28	5603.5
29	6623.0
30	7810.5
31	8998.0
32	10110.5
33	11223.0
34	12991.0
35	14759.0
36	15762.5
37	16766.0
38	18937.5
39	21109.0
40	23345.5
41	25582.0
42	29693.0
43	33804.0
44	42899.5
45	51995.0
46	50642.0
47	49289.0
48	48613.5
49	47938.0
50	43196.5
51	38455.0
52	36153.5
53	33852.0
54	31463.5
55	29075.0
56	28461.0
57	27847.0
58	27186.0
59	26525.0
60	25176.0
61	23827.0
62	22796.5
63	21766.0
64	18510.0
65	15254.0
66	12844.0
67	10434.0
68	8533.0
69	6632.0
70	5690.0
71	4748.0
72	3779.5
73	2811.0
74	2258.5
75	1294.0
76	882.0
77	601.0
78	320.0
79	302.5
80	285.0
81	165.5
82	46.0
83	35.5
84	25.0
85	15.5
86	6.0
87	6.5
88	7.0
89	6.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	572016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.50755062788237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49629134536204	23.471956711534695
2	9.015929244771737	5.6813969427410145
3	3.5179179778994185	3.3252293638021047
4	1.9806224303513544	2.496182459960589
5	1.2644101881497152	1.9919234008768705
6	0.9798084266836201	1.8522818165615957
7	0.7237672669134337	1.5962893663555344
8	0.5766215610965664	1.4534346423502904
9	0.5084197101170439	1.4417153781053433
>10	6.673086871565542	46.94905388594692
>50	0.21343614021426027	4.110342175994984
>100	0.04347773226586767	2.4494109712213796
>500	0.0033878752414961827	0.6929441990780996
>1k	0.0028232293679134853	2.4878386854706402
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3840	0.6713098934295545	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	3017	0.5274327990825431	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG	2776	0.4853011104584487	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC	2535	0.44316942183435426	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	1816	0.31747363710106014	No Hit
GAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	761	0.13303823669267992	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	678	0.11852815305865569	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	636	0.11118570109926995	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	631	0.11031159967553356	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	618	0.1080389359738189	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.748202847472798E-4	0.0	0.0	0.1220245587536013	0.0
2	1.748202847472798E-4	0.0	0.0	0.7029523649688121	0.0
3	1.748202847472798E-4	0.0	0.0	0.9314424771335068	0.0
4	1.748202847472798E-4	0.0	0.0	1.3221658135436771	0.0
5	1.748202847472798E-4	0.0	0.0	2.570907107493497	0.0
6	1.748202847472798E-4	0.0	0.0	3.267915582780901	0.0
7	1.748202847472798E-4	0.0	0.0	3.9574067858241726	0.0
8	1.748202847472798E-4	0.0	0.0	4.891646387513636	0.0
9	1.748202847472798E-4	0.0	0.0	5.273453889401695	0.0
10	3.496405694945596E-4	0.0	0.0	6.587752790131745	0.0
11	3.496405694945596E-4	0.0	0.0	7.718315571592403	0.0
12	3.496405694945596E-4	0.0	0.0	9.047299376241224	0.0
13	3.496405694945596E-4	0.0	0.0	9.444840703756539	0.0
14	3.496405694945596E-4	0.0	0.0	9.610919974266453	0.0
15	3.496405694945596E-4	0.0	0.0	9.947274202120221	0.0
16	3.496405694945596E-4	0.0	0.0	10.480825711168919	0.0
17	5.244608542418394E-4	0.0	0.0	11.099864339459035	0.0
18	5.244608542418394E-4	0.0	0.0	11.742503706190037	0.0
19	5.244608542418394E-4	0.0	0.0	12.227280355794244	0.0
20	6.992811389891192E-4	0.0	0.0	12.658212357696287	0.0
21	8.74101423736399E-4	0.0	0.0	13.160820676344718	0.0
22	8.74101423736399E-4	0.0	0.0	13.708532628457945	0.0
23	8.74101423736399E-4	0.0	0.0	14.243133059215127	0.0
24	8.74101423736399E-4	0.0	0.0	14.648890940113564	0.0
25	8.74101423736399E-4	0.0	0.0	15.04363514307292	0.0
26	0.0010489217084836788	0.0	0.0	15.40166708623535	0.0
27	0.0010489217084836788	0.0	0.0	15.767740762496153	0.0
28	0.0010489217084836788	0.0	0.0	16.139233867584124	0.0
29	0.0010489217084836788	0.0	0.0	16.5199924477637	0.0
30	0.0010489217084836788	0.0	0.0	16.98064389807278	0.0
31	0.0010489217084836788	0.0	0.0	17.372241335906686	0.0
32	0.0010489217084836788	0.0	0.0	17.759992727476156	0.0
33	0.0010489217084836788	0.0	0.0	18.152813907303294	0.0
34	0.0010489217084836788	0.0	0.0	18.51049620989623	0.0
35	0.0010489217084836788	0.0	0.0	18.88373751783167	0.0
36	0.0012237419932309586	0.0	0.0	19.252433498363683	0.0
37	0.0012237419932309586	0.0	0.0	19.624625884590642	0.0
38	0.0012237419932309586	0.0	0.0	20.01534922100081	0.0
39	0.0012237419932309586	0.0	0.0	20.38946463035999	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	25	3.888339E-5	45.000004	40
TTGTCGC	25	3.888339E-5	45.000004	38
TACGGGA	140	0.0	45.000004	4
TTGCGAC	25	3.888339E-5	45.000004	16
TGTCGCA	25	3.888339E-5	45.000004	39
CGGAACA	20	7.030312E-4	45.0	13
ACCGGTA	20	7.030312E-4	45.0	41
AACCCGT	20	7.030312E-4	45.0	43
GTCGTCG	20	7.030312E-4	45.0	42
ACCCGTT	20	7.030312E-4	45.0	44
ACGTAAG	20	7.030312E-4	45.0	1
GCTACGA	40	6.8048394E-9	45.0	10
GTGCGGT	20	7.030312E-4	45.0	31
TACGTTC	20	7.030312E-4	45.0	41
TGCGTTG	20	7.030312E-4	45.0	1
TGCGTCG	20	7.030312E-4	45.0	2
TACCGGA	20	7.030312E-4	45.0	21
TCCGATC	30	2.1635824E-6	44.999996	14
CCCCGTA	30	2.1635824E-6	44.999996	40
CATACGG	30	2.1635824E-6	44.999996	2
>>END_MODULE