FastQCFastQC Report
Sat 14 Jan 2017
SRR2936001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925010
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.3393476827277543No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC17810.19253845904368602No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC11390.1231338039588761No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC9430.10194484383952607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG302.1650394E-645.0000041
TTCGACC253.8902133E-545.022
TGCGTTG207.0325733E-445.01
CGTTTTT20150.041.761791
GCTACGA603.6379788E-1241.25000410
GTTAACG556.002665E-1140.9090921
ACGGGTA1300.039.8076935
GTTTGCG800.039.3751
CATTGCG403.457244E-739.3751
TTGCGCG403.457244E-739.3751
GCGTTAG750.039.0000041
CGAGACA1100.038.86363622
TAAGGGA12000.038.6250044
ACGGGCC1050.038.571435
CGTAAGG1350.038.3333362
CGTTATT2650.038.2075461
AACGGGA4250.037.5882384
GCGAACG301.1397495E-437.5000041
GCAACGC301.1397495E-437.50000413
CGAATCC301.1397495E-437.50000422
Produced by FastQC (version 0.11.5)