FastQCFastQC Report
Sat 14 Jan 2017
SRR2936000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216929
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206661.6982091806506376No Hit
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC41350.3397897494430653No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.19738209870912765No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG23730.1949990508895753No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC20410.16771726205883827No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC13960.11471499158948469No Hit
GAACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT12940.10633323718967994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG351.2120108E-745.0000041
ATTTACG253.890942E-545.01
TAATGCG253.890942E-545.01
CGTTTTT97900.043.712971
CGTTATT13600.042.5183831
GCGATAT603.6379788E-1241.2500049
TACGGGA4050.039.4444434
CGTATGG1100.038.863642
TATAGCG356.2485906E-638.571431
ACGTAAG850.037.0588261
GTTATTT16300.036.5797542
CGAATAT800.036.562514
TACGGGT2100.036.428574
ACAACGA5200.036.34615313
AGGGCGA11150.036.121086
TAACGGG3800.036.118423
CTCACGA504.879439E-836.024
TCTTGCG504.879439E-836.01
TACGCGA250.002107157436.038
TACTACG250.002107157436.01
Produced by FastQC (version 0.11.5)