##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936000.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1216929
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09454372440791	33.0	31.0	34.0	30.0	34.0
2	32.23653968308751	34.0	31.0	34.0	30.0	34.0
3	32.09459878102995	33.0	31.0	34.0	30.0	34.0
4	35.782495116806324	37.0	35.0	37.0	35.0	37.0
5	31.99349838815576	37.0	35.0	37.0	0.0	37.0
6	33.82948881980789	37.0	35.0	37.0	17.0	37.0
7	35.51291653005229	37.0	35.0	37.0	32.0	37.0
8	35.78076863974809	37.0	35.0	37.0	35.0	37.0
9	37.758173237715596	39.0	38.0	39.0	35.0	39.0
10	37.3923630713049	39.0	37.0	39.0	34.0	39.0
11	37.35230157223634	39.0	37.0	39.0	34.0	39.0
12	37.306099205458985	39.0	37.0	39.0	34.0	39.0
13	37.26151320249579	39.0	37.0	39.0	34.0	39.0
14	38.49422932644386	40.0	38.0	41.0	34.0	41.0
15	38.63038599622492	40.0	38.0	41.0	34.0	41.0
16	38.5803263789424	40.0	38.0	41.0	34.0	41.0
17	38.55757977663446	40.0	38.0	41.0	34.0	41.0
18	38.369979678354284	40.0	38.0	41.0	34.0	41.0
19	38.1105126100208	40.0	37.0	41.0	34.0	41.0
20	38.04944988573697	40.0	36.0	41.0	34.0	41.0
21	37.95551424939335	40.0	36.0	41.0	34.0	41.0
22	38.00910324267069	40.0	35.0	41.0	34.0	41.0
23	38.017656740861625	40.0	35.0	41.0	34.0	41.0
24	37.959662396080624	40.0	35.0	41.0	34.0	41.0
25	37.70031283665686	40.0	35.0	41.0	33.0	41.0
26	37.73351444496762	40.0	35.0	41.0	34.0	41.0
27	37.715130463650716	40.0	35.0	41.0	34.0	41.0
28	37.60534920278833	40.0	35.0	41.0	33.0	41.0
29	37.51610488368672	40.0	35.0	41.0	33.0	41.0
30	37.292768929000786	40.0	35.0	41.0	33.0	41.0
31	37.169742030964834	40.0	35.0	41.0	33.0	41.0
32	36.914959705948334	40.0	35.0	41.0	32.0	41.0
33	36.621285218776116	40.0	35.0	41.0	31.0	41.0
34	36.30468992028294	40.0	35.0	41.0	30.0	41.0
35	36.15266461724554	40.0	35.0	41.0	29.0	41.0
36	35.98717180706516	39.0	35.0	41.0	27.0	41.0
37	35.91801986804489	39.0	35.0	41.0	27.0	41.0
38	35.74403765544251	39.0	35.0	41.0	26.0	41.0
39	35.64599413770237	39.0	35.0	41.0	25.0	41.0
40	35.551331260903474	39.0	35.0	41.0	25.0	41.0
41	35.415802400961766	39.0	35.0	41.0	24.0	41.0
42	35.39436318799207	39.0	35.0	41.0	24.0	41.0
43	35.30422645856907	39.0	35.0	41.0	23.0	41.0
44	35.242490728711374	39.0	35.0	41.0	23.0	41.0
45	35.19746755973438	39.0	35.0	41.0	23.0	41.0
46	35.077770354720776	38.0	35.0	41.0	23.0	41.0
47	35.01029558832109	38.0	35.0	41.0	23.0	41.0
48	34.96130587733549	38.0	35.0	41.0	23.0	41.0
49	34.90215534349169	38.0	35.0	41.0	23.0	41.0
50	34.794608395395294	38.0	34.0	40.0	23.0	41.0
51	33.885959657465634	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	24.0
9	44.0
10	56.0
11	34.0
12	35.0
13	34.0
14	46.0
15	70.0
16	105.0
17	231.0
18	487.0
19	815.0
20	1498.0
21	2454.0
22	3994.0
23	5865.0
24	9515.0
25	15233.0
26	20904.0
27	23102.0
28	21619.0
29	20439.0
30	20801.0
31	23859.0
32	29661.0
33	41989.0
34	69449.0
35	87830.0
36	92013.0
37	136345.0
38	220044.0
39	368024.0
40	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.779019153952284	17.514579733082208	20.979366914585814	19.72703419837969
2	37.96302002828431	22.46926484618248	22.750217966701427	16.81749715883178
3	29.852522209594806	23.042100237565215	31.49846868634078	15.606908866499197
4	25.39400408733788	23.837216468668263	32.771755788546415	17.997023655447443
5	21.395742890505527	35.31726173014202	27.5264210155235	15.76057436382895
6	23.28360980796743	32.24814266074685	30.352633555449827	14.115613975835895
7	74.34961283690339	5.242458680826901	16.265369631260327	4.142558851009385
8	75.91954830561191	5.312881852597809	14.930287633871819	3.8372822079184568
9	70.55563636005058	6.156398606656592	16.957932632059883	6.330032401232939
10	37.09123539664188	24.56396387956898	23.602609519536472	14.742191204252672
11	28.0617028602326	24.46765587803397	30.20932199002571	17.261319271707716
12	24.087354315658512	22.123805086410137	34.635627879687306	19.15321271824404
13	23.151227392888163	22.60320856845387	36.443457260037356	17.80210677862061
14	19.828436991804782	24.961028950744048	35.80619740346396	19.404336653987208
15	19.40647317961853	23.370467792286977	38.00566836684802	19.217390661246466
16	23.06987507077241	23.654790049378395	34.27874592519367	18.99658895465553
17	22.929932641920768	23.876824366910476	33.375077757206874	19.81816523396188
18	24.498799847813636	22.279689283433957	33.814955515071134	19.406555353681274
19	22.600003780006887	25.301229570500826	32.17246034896037	19.926306300531913
20	24.040925970208615	24.903507106823817	32.38800291553574	18.66756400743182
21	23.105785136191184	25.755734311533374	33.3524798899525	17.786000662322945
22	22.896980842760755	22.573708079929066	34.08629427024913	20.443016807061053
23	21.1447011288251	25.32949744808448	33.411480867002105	20.114320556088316
24	20.999746082146125	24.148245296151213	34.28244375801711	20.569564863685557
25	21.507746138024487	26.040138742687535	32.457357824491	19.994757294796987
26	20.65379327799732	24.974176800782956	32.901755155806136	21.470274765413595
27	21.21775387060379	24.09220258536036	33.26677234251135	21.423271201524493
28	19.931483266484733	25.79016524382277	33.42619002423313	20.852161465459364
29	21.123418046574617	25.179447609515428	32.89633166766508	20.800802676244874
30	21.525249213388783	24.775726439258168	33.11516119675018	20.58386315060287
31	22.20573262696509	25.69706203073474	30.684534594869543	21.412670747430624
32	21.63371897620979	26.206048175366025	31.169115042866103	20.99111780555809
33	21.529686612776917	26.029127418279952	29.850385683963488	22.59080028497965
34	19.85958096158445	27.770313633745268	31.051770481268832	21.318334923401448
35	20.34794141646719	27.485580506340142	29.812421266976134	22.354056810216537
36	20.697838575627667	29.34928824935555	29.073183398538454	20.879689776478333
37	20.49223907064422	27.994977521285136	29.123556099000027	22.38922730907062
38	20.43537461922594	28.3950830327817	28.942444464714047	22.22709788327832
39	21.16894247733434	26.64551506291657	29.326443859912942	22.859098599836145
40	22.60937162315961	25.99872301506497	29.807983867588	21.583921494187415
41	19.889656668548454	26.890311595828514	29.997970300650245	23.222061434972787
42	21.26681178606147	26.286085712477885	30.393720586821416	22.05338191463923
43	21.051762263862557	25.704046826067913	30.580337883311188	22.66385302675834
44	21.387936354544923	25.8549184052644	29.968305463999954	22.788839776190724
45	21.0796192711325	25.474123798512483	29.55562732090368	23.890629609451334
46	21.377828944827513	26.309998364736153	29.213043653327354	23.099129037108984
47	19.91176149142637	26.98456524579495	31.17470287913264	21.928970383646046
48	20.54696699643118	26.24524520329452	30.74452166067207	22.46326613960223
49	21.44800559441019	25.524743021162287	30.754793418514964	22.272457965912558
50	19.722843321179788	26.29339920406203	31.122440175227972	22.861317299530214
51	20.056387841854374	25.188651104542664	30.447544597918203	24.30741645568476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	677.0
1	1240.0
2	1803.0
3	18951.5
4	36100.0
5	23412.5
6	10725.0
7	10281.5
8	9838.0
9	9742.0
10	9646.0
11	9457.0
12	9268.0
13	9054.5
14	8841.0
15	8424.5
16	8008.0
17	7547.5
18	7087.0
19	6769.0
20	6451.0
21	6529.5
22	6608.0
23	6992.5
24	7377.0
25	8002.5
26	10094.0
27	11560.0
28	13388.0
29	15216.0
30	17251.0
31	19286.0
32	22555.0
33	25824.0
34	28865.0
35	31906.0
36	35140.0
37	38374.0
38	41184.0
39	43994.0
40	48481.5
41	52969.0
42	59623.0
43	66277.0
44	79046.0
45	91815.0
46	92027.0
47	92239.0
48	94193.5
49	96148.0
50	89013.0
51	81878.0
52	78401.0
53	74924.0
54	71661.0
55	68398.0
56	64346.5
57	60295.0
58	55972.5
59	51650.0
60	47851.0
61	44052.0
62	39520.0
63	34988.0
64	30950.0
65	26912.0
66	23536.5
67	20161.0
68	16933.5
69	13706.0
70	11072.5
71	8439.0
72	7268.5
73	6098.0
74	5058.5
75	3345.0
76	2671.0
77	1877.0
78	1083.0
79	801.5
80	520.0
81	400.5
82	281.0
83	211.0
84	141.0
85	82.5
86	24.0
87	16.0
88	8.0
89	9.0
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1216929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.760154345978734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50182107584352	24.40691157501125
2	8.6179130738849	5.646483248813946
3	3.503180604399204	3.442942119058709
4	2.061211827949289	2.701024704935026
5	1.3825546120079923	2.2646351240563285
6	1.014462672669935	1.9940372240940707
7	0.8403218731280483	1.9270351984783778
8	0.6529912252663855	1.7113674661037244
9	0.5613598005545837	1.6551210338816398
>10	6.6920477648023375	45.92659154356712
>50	0.1361692007479573	2.7202120903368834
>100	0.0303144265357073	1.8213382701567014
>500	0.0035966268610113502	0.7950733604732714
>1k	0.0017983134305056751	1.2479462039904379
>5k	0.0	0.0
>10k+	2.569019186436679E-4	1.7392808370425135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20666	1.6982091806506376	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	4135	0.3397897494430653	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2402	0.19738209870912765	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG	2373	0.1949990508895753	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC	2041	0.16771726205883827	No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1396	0.11471499158948469	No Hit
GAACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	1294	0.10633323718967994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.108703137159193E-4	0.0	0.0	0.06105532861818561	0.0
2	4.108703137159193E-4	0.0	0.0	0.27331093268382955	0.0
3	4.108703137159193E-4	0.0	0.0	0.43182469971543125	0.0
4	4.108703137159193E-4	0.0	0.0	0.7134352127363224	0.0
5	4.108703137159193E-4	0.0	0.0	1.49540359380046	0.0
6	4.108703137159193E-4	0.0	0.0	2.28904069177413	0.0
7	4.108703137159193E-4	0.0	0.0	2.901977025775538	0.0
8	4.108703137159193E-4	0.0	0.0	3.7450829095206046	0.0
9	4.108703137159193E-4	0.0	0.0	4.235744238160156	0.0
10	4.108703137159193E-4	0.0	0.0	5.158641136828853	0.0
11	4.108703137159193E-4	0.0	0.0	5.86081850296936	0.0
12	4.108703137159193E-4	0.0	0.0	6.606548122363753	0.0
13	4.108703137159193E-4	0.0	0.0	6.941900472418687	0.0
14	4.108703137159193E-4	0.0	0.0	7.147993021778592	0.0
15	4.108703137159193E-4	0.0	0.0	7.351866871444431	0.0
16	4.108703137159193E-4	0.0	0.0	7.638654350418142	0.0
17	4.108703137159193E-4	0.0	0.0	7.966939731077162	0.0
18	4.108703137159193E-4	0.0	0.0	8.339023887178298	0.0
19	4.108703137159193E-4	0.0	0.0	8.60592524296816	0.0
20	4.108703137159193E-4	0.0	0.0	8.860007444970085	0.0
21	4.930443764591032E-4	0.0	0.0	9.144329702061501	0.0
22	4.930443764591032E-4	0.0	0.0	9.465219417073634	0.0
23	4.930443764591032E-4	0.0	0.0	9.783643910203471	0.0
24	4.930443764591032E-4	0.0	0.0	10.056050928197125	0.0
25	5.752184392022871E-4	0.0	0.0	10.303394857054109	0.0
26	5.752184392022871E-4	0.0	0.0	10.548766608405256	0.0
27	5.752184392022871E-4	0.0	0.0	10.825364503598813	0.0
28	5.752184392022871E-4	0.0	0.0	11.098428914094413	0.0
29	5.752184392022871E-4	0.0	0.0	11.388256833389622	0.0
30	5.752184392022871E-4	0.0	0.0	11.782363638305933	0.0
31	6.573925019454709E-4	0.0	0.0	12.102760308941606	0.0
32	6.573925019454709E-4	0.0	0.0	12.44846659090218	0.0
33	6.573925019454709E-4	0.0	0.0	12.770917613106434	0.0
34	6.573925019454709E-4	0.0	0.0	13.112433017867106	0.0
35	6.573925019454709E-4	0.0	0.0	13.44753884573381	0.0
36	6.573925019454709E-4	0.0	0.0	13.757088540087384	0.0
37	7.395665646886548E-4	0.0	0.0	14.077074340409341	0.0
38	7.395665646886548E-4	0.0	0.0	14.44250239742828	0.0
39	7.395665646886548E-4	0.0	0.0	14.936615036703046	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	35	1.2120108E-7	45.000004	1
ATTTACG	25	3.890942E-5	45.0	1
TAATGCG	25	3.890942E-5	45.0	1
CGTTTTT	9790	0.0	43.71297	1
CGTTATT	1360	0.0	42.518383	1
GCGATAT	60	3.6379788E-12	41.250004	9
TACGGGA	405	0.0	39.444443	4
CGTATGG	110	0.0	38.86364	2
TATAGCG	35	6.2485906E-6	38.57143	1
ACGTAAG	85	0.0	37.058826	1
GTTATTT	1630	0.0	36.579754	2
CGAATAT	80	0.0	36.5625	14
TACGGGT	210	0.0	36.42857	4
ACAACGA	520	0.0	36.346153	13
AGGGCGA	1115	0.0	36.12108	6
TAACGGG	380	0.0	36.11842	3
CTCACGA	50	4.879439E-8	36.0	24
TCTTGCG	50	4.879439E-8	36.0	1
TACGCGA	25	0.0021071574	36.0	38
TACTACG	25	0.0021071574	36.0	1
>>END_MODULE