##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935998.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	982633
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07077515206593	33.0	31.0	34.0	30.0	34.0
2	32.18515966795334	33.0	31.0	34.0	30.0	34.0
3	32.1383252954053	33.0	31.0	34.0	30.0	34.0
4	35.761896862816535	37.0	35.0	37.0	35.0	37.0
5	32.08950340564585	37.0	35.0	37.0	0.0	37.0
6	33.86959933159175	37.0	35.0	37.0	17.0	37.0
7	35.500300722650266	37.0	35.0	37.0	32.0	37.0
8	35.728760381546316	37.0	35.0	37.0	35.0	37.0
9	37.67902767360754	39.0	37.0	39.0	35.0	39.0
10	37.33092314221077	39.0	37.0	39.0	34.0	39.0
11	37.24930060358241	39.0	37.0	39.0	34.0	39.0
12	37.237854824741284	39.0	37.0	39.0	34.0	39.0
13	37.18771097652939	39.0	37.0	39.0	33.0	39.0
14	38.42300329828125	40.0	38.0	41.0	34.0	41.0
15	38.51847536160499	40.0	38.0	41.0	34.0	41.0
16	38.517305036570114	40.0	38.0	41.0	34.0	41.0
17	38.45112366468458	40.0	38.0	41.0	34.0	41.0
18	38.3580370290841	40.0	38.0	41.0	34.0	41.0
19	38.215527058423646	40.0	37.0	41.0	34.0	41.0
20	38.15606538758621	40.0	37.0	41.0	34.0	41.0
21	38.17647076782481	40.0	37.0	41.0	34.0	41.0
22	38.24054250162573	40.0	37.0	41.0	34.0	41.0
23	38.201634791422634	40.0	37.0	41.0	34.0	41.0
24	38.19076298068557	40.0	37.0	41.0	34.0	41.0
25	38.0260198873842	40.0	36.0	41.0	34.0	41.0
26	38.02043387510902	40.0	37.0	41.0	34.0	41.0
27	37.96937106732626	40.0	37.0	41.0	34.0	41.0
28	37.89840357488503	40.0	36.0	41.0	33.0	41.0
29	37.89326432147099	40.0	36.0	41.0	33.0	41.0
30	37.724824018733344	40.0	36.0	41.0	33.0	41.0
31	37.728443884949925	40.0	36.0	41.0	33.0	41.0
32	37.56291005899456	40.0	36.0	41.0	33.0	41.0
33	37.45489312897084	40.0	36.0	41.0	33.0	41.0
34	37.27009575294133	40.0	36.0	41.0	32.0	41.0
35	37.16969102401405	40.0	36.0	41.0	32.0	41.0
36	37.0880522026026	40.0	36.0	41.0	31.0	41.0
37	37.011144547353894	40.0	36.0	41.0	31.0	41.0
38	36.97612638696238	40.0	35.0	41.0	31.0	41.0
39	36.84676069295454	40.0	35.0	41.0	31.0	41.0
40	36.80586546554003	40.0	35.0	41.0	31.0	41.0
41	36.7235325904992	40.0	35.0	41.0	30.0	41.0
42	36.704893892226295	40.0	35.0	41.0	31.0	41.0
43	36.64074481520568	40.0	35.0	41.0	31.0	41.0
44	36.66068511845216	40.0	35.0	41.0	31.0	41.0
45	36.65071903752469	40.0	35.0	41.0	31.0	41.0
46	36.53783253768192	39.0	35.0	41.0	31.0	41.0
47	36.39856182318322	39.0	35.0	41.0	30.0	41.0
48	36.40751531853703	39.0	35.0	41.0	30.0	41.0
49	36.37707363786887	39.0	35.0	41.0	30.0	41.0
50	36.26030980030184	39.0	35.0	41.0	30.0	41.0
51	35.31495482036529	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	36.0
10	48.0
11	43.0
12	21.0
13	27.0
14	26.0
15	41.0
16	64.0
17	130.0
18	275.0
19	585.0
20	1160.0
21	2206.0
22	3752.0
23	4876.0
24	6213.0
25	7687.0
26	9194.0
27	9947.0
28	10457.0
29	11269.0
30	13347.0
31	17518.0
32	22447.0
33	31966.0
34	52441.0
35	68037.0
36	67119.0
37	106479.0
38	185500.0
39	349436.0
40	272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4758796010311	18.376647232486594	22.64395761184491	13.503515554637389
2	31.710109471186087	27.166907685778924	24.38071996360798	16.74226287942701
3	30.51820974870577	27.489713860617343	26.439983188026456	15.552093202650429
4	26.509286783570264	23.972429177526095	31.173388233450332	18.344895805453305
5	24.24974532709567	35.5595629293948	22.85787267474225	17.33281906876728
6	23.91096167134627	36.5381581933438	25.591955491012413	13.958924644297516
7	76.66504178060374	5.334850345958257	13.798742765610356	4.201365107827643
8	77.88482576913253	8.18912045494096	9.580586037717032	4.345467738209484
9	72.19328070602148	7.2253832305652255	10.964724368100805	9.616611695312493
10	37.73758870300509	31.00262254575208	18.099432850311356	13.160355900931478
11	27.09933413593885	24.099027816081893	30.10544119727304	18.696196850706215
12	24.38906488994365	22.379871223539205	31.78694385391087	21.44412003260627
13	24.46864699231554	23.81102609010689	34.030813131657496	17.689513785920074
14	19.755391890970483	29.140380996770922	30.84111769093853	20.263109421320067
15	18.432313997189183	25.534660448000423	35.62225164430667	20.410773910503718
16	21.838570453058264	25.960048156331002	30.70617412604706	21.495207264563675
17	22.200862376899615	26.220063848863205	30.463662425340893	21.11541134889628
18	22.41620218331768	23.79698218968832	31.938882573656695	21.84793305333731
19	21.69070242908594	26.630389982831844	29.5568131743998	22.122094413682422
20	23.9898314019578	26.032811843282282	30.982981438644945	18.99437531611497
21	22.43177259465131	28.378346747972028	30.975450651463976	18.214430005912686
22	21.59799233284451	23.1025214907295	32.330381739672895	22.969104436753092
23	21.090681872072278	26.908825573739126	31.094925572416148	20.90556698177244
24	20.874527926499518	26.071585220524856	31.058391077849002	21.995495775126624
25	20.044818360466216	30.113786123608712	28.938779788588416	20.902615727336656
26	20.81519753560078	26.51925998821534	30.27040614349406	22.395136332689823
27	21.834092687707415	26.272474056946997	30.323019886366527	21.57041336897906
28	18.33359962468185	27.311926222709804	31.65332326514579	22.70115088746256
29	22.050348400674512	24.499787814982806	31.792032223627743	21.657831560714936
30	21.554639422856752	26.233497144915752	31.92025914049294	20.291604291734554
31	22.134306501002918	24.995395025406232	28.968088798157606	23.90220967543325
32	24.057812021375224	26.285398516027858	29.583781533899227	20.07300792869769
33	23.07117713327356	24.78443121694468	29.633138720152896	22.511252929628863
34	22.355345281503876	24.70474734717845	30.775477721590867	22.164429649726806
35	21.134034782060038	26.30707497102173	31.270169025465254	21.288721221452974
36	22.029587852229675	26.806142272852636	30.2335663467439	20.930703528173794
37	19.658916401138573	26.50918501617593	32.6558338667641	21.17606471592141
38	20.909128840574255	26.757192156176313	28.928704816549004	23.404974186700425
39	21.230713806680622	25.700235998587466	31.865304747550717	21.203745447181195
40	22.2070701879542	25.808923575739872	30.587411576855246	21.39659465945068
41	19.874154440162297	25.948751975559542	31.172370559506955	23.004723024771202
42	21.240687011325694	26.147808998883608	31.06103703010178	21.55046695968892
43	21.790027405959293	25.227119382312623	30.032066905955734	22.950786305772347
44	21.447376589225073	24.31507999426032	31.05808577566599	23.179457640848618
45	20.984742014566983	24.538459424831043	29.458811173652826	25.017987386949148
46	23.097534888406965	25.738297004069675	29.93080834858996	21.233359758933396
47	19.51338902723601	25.879244845227056	33.33441885220626	21.272947275330665
48	20.56892044130413	25.79223372306853	30.730801835476722	22.908044000150614
49	20.697249125563662	24.728866219636426	32.624794811491164	21.94908984330874
50	20.550500542929047	25.440525608238275	31.008219752440635	23.00075409639204
51	20.29587852229673	24.521973106948373	29.827717978126117	25.354430392628785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	540.0
1	1088.0
2	1636.0
3	6261.5
4	10887.0
5	7178.0
6	3469.0
7	3275.5
8	3082.0
9	3069.0
10	3056.0
11	3099.5
12	3143.0
13	3019.5
14	2896.0
15	2932.0
16	2968.0
17	2892.5
18	2817.0
19	3035.5
20	3254.0
21	3709.5
22	4165.0
23	4274.5
24	4384.0
25	5514.5
26	8549.5
27	10454.0
28	11946.0
29	13438.0
30	15528.5
31	17619.0
32	19482.0
33	21345.0
34	25140.5
35	28936.0
36	32953.5
37	36971.0
38	40940.0
39	44909.0
40	48529.0
41	52149.0
42	59563.5
43	66978.0
44	87052.5
45	107127.0
46	97160.5
47	87194.0
48	87607.5
49	88021.0
50	80297.0
51	72573.0
52	67144.0
53	61715.0
54	57445.5
55	53176.0
56	48734.0
57	44292.0
58	39716.5
59	35141.0
60	31927.0
61	28713.0
62	25114.0
63	21515.0
64	17759.5
65	14004.0
66	11585.5
67	9167.0
68	7596.0
69	6025.0
70	4692.0
71	3359.0
72	2663.0
73	1967.0
74	1660.0
75	1117.5
76	882.0
77	650.5
78	419.0
79	273.5
80	128.0
81	97.5
82	67.0
83	43.0
84	19.0
85	21.5
86	24.0
87	13.5
88	3.0
89	3.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	982633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.3534279901873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.512068118371	21.28477769932159
2	9.961504353610351	5.8480860143527735
3	3.892746939252264	3.4279640089609003
4	2.149049881726957	2.52327923802371
5	1.367951376410795	2.007703111077593
6	1.0016949460245141	1.7641908279759078
7	0.7769041301400553	1.596335960754165
8	0.5963886858974724	1.4004841876523106
9	0.5138900460099178	1.3575991014383544
>10	6.865634962708661	46.466179566233066
>50	0.31962079476421595	5.717631934193071
>100	0.0355134072186753	1.716723617478096
>500	0.0031645610392873414	0.7172195285188464
>1k	0.0024613252527790432	1.1577279394727156
>5k	0.0014064715730165963	3.014097264546823
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	8280	0.8426340251141575	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	8155	0.8299131008219752	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGC	6758	0.6877440509325455	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6010	0.6116220399681264	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2862	0.29125828259380665	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	2043	0.207910786631428	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	1522	0.15488997418161204	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTC	1322	0.13453649531412032	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTA	1189	0.12100143186723833	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1165	0.11855901440313932	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTGTATT	1114	0.11336887729192892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0176739433745865E-4	0.0	0.0	0.09454190933949908	0.0
2	1.0176739433745865E-4	0.0	0.0	0.6148785965869251	0.0
3	1.0176739433745865E-4	0.0	0.0	0.8056924609696601	0.0
4	1.0176739433745865E-4	0.0	0.0	1.2122532013478073	0.0
5	1.0176739433745865E-4	0.0	0.0	2.6629474076282804	0.0
6	1.0176739433745865E-4	0.0	0.0	3.3042855267429445	0.0
7	1.0176739433745865E-4	0.0	0.0	3.9669947986684755	0.0
8	1.0176739433745865E-4	0.0	0.0	4.847995131447855	0.0
9	1.0176739433745865E-4	0.0	0.0	5.163677588682652	0.0
10	2.035347886749173E-4	0.0	0.0	6.730386624507828	0.0
11	2.035347886749173E-4	0.0	0.0	7.654332797697615	0.0
12	3.0530218301237595E-4	0.0	0.0	9.16659627755225	0.0
13	3.0530218301237595E-4	0.0	0.0	9.521968018578656	0.0
14	3.0530218301237595E-4	0.0	0.0	9.693853147614623	0.0
15	3.0530218301237595E-4	0.0	0.0	10.037826940475233	0.0
16	3.0530218301237595E-4	0.0	0.0	10.494966075839098	0.0
17	3.0530218301237595E-4	0.0	0.0	11.0731066430702	0.0
18	3.0530218301237595E-4	0.0	0.0	11.64453056227503	0.0
19	4.070695773498346E-4	0.0	0.0	12.17514575635054	0.0
20	4.070695773498346E-4	0.0	0.0	12.568273200676142	0.0
21	4.070695773498346E-4	0.0	0.0	13.01411615526855	0.0
22	5.088369716872933E-4	0.0	0.0	13.55551869314383	0.0
23	5.088369716872933E-4	0.0	0.0	14.046749905610742	0.0
24	5.088369716872933E-4	0.0	0.0	14.428988238742237	0.0
25	5.088369716872933E-4	0.0	0.0	14.76726305751995	0.0
26	5.088369716872933E-4	0.0	0.0	15.104520202354287	0.0
27	5.088369716872933E-4	0.0	0.0	15.476072959080348	0.0
28	5.088369716872933E-4	0.0	0.0	15.818011404054209	0.0
29	6.106043660247519E-4	0.0	0.0	16.192108345638708	0.0
30	7.123717603622105E-4	0.0	0.0	16.654335850719445	0.0
31	7.123717603622105E-4	0.0	0.0	17.04329083187721	0.0
32	7.123717603622105E-4	0.0	0.0	17.421662004023883	0.0
33	8.141391546996692E-4	0.0	0.0	17.787515786667047	0.0
34	8.141391546996692E-4	0.0	0.0	18.156727893323346	0.0
35	8.141391546996692E-4	0.0	0.0	18.560540914054382	0.0
36	8.141391546996692E-4	0.0	0.0	18.92385051183911	0.0
37	8.141391546996692E-4	0.0	0.0	19.291230805397337	0.0
38	8.141391546996692E-4	0.0	0.0	19.704101124224405	0.0
39	8.141391546996692E-4	0.0	0.0	20.191770477889506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	25	3.8903905E-5	45.000004	1
TTTACGT	20	7.032787E-4	45.0	24
GCTATCG	20	7.032787E-4	45.0	15
TACGGAC	20	7.032787E-4	45.0	4
CAATCGA	55	1.8189894E-12	45.0	41
GAGATCG	35	1.2116834E-7	45.0	33
TAATACG	265	0.0	43.301888	4
CGATGAA	1325	0.0	41.773586	19
CCGATGA	1245	0.0	41.566265	18
CGTTTTT	2800	0.0	41.383926	1
GTCGAAG	60	3.6379788E-12	41.249996	1
GTTAGCG	45	1.927765E-8	40.0	1
CCGTCTA	40	3.457535E-7	39.375	41
CGAACGG	120	0.0	39.374996	2
ATCGAAT	115	0.0	39.130436	43
CGGCGAA	110	0.0	38.863636	31
AGTACGG	110	0.0	38.863636	2
CTACGAA	175	0.0	38.571426	11
CGTAAGA	35	6.2473664E-6	38.571426	30
TACGGCT	830	0.0	38.22289	7
>>END_MODULE