##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935997.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	712173
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14961252392326	33.0	31.0	34.0	30.0	34.0
2	32.29299341592562	34.0	31.0	34.0	30.0	34.0
3	32.16360350645138	33.0	31.0	34.0	30.0	34.0
4	35.826582866803435	37.0	35.0	37.0	35.0	37.0
5	32.09936349735247	37.0	35.0	37.0	0.0	37.0
6	33.9126083128678	37.0	35.0	37.0	17.0	37.0
7	35.582138047918136	37.0	35.0	37.0	32.0	37.0
8	35.85385152203187	37.0	35.0	37.0	35.0	37.0
9	37.844335575766	39.0	38.0	39.0	35.0	39.0
10	37.452397100142804	39.0	37.0	39.0	35.0	39.0
11	37.396850203532004	39.0	37.0	39.0	35.0	39.0
12	37.37870713997863	39.0	37.0	39.0	35.0	39.0
13	37.3553167559006	39.0	37.0	39.0	35.0	39.0
14	38.580307032139665	40.0	38.0	41.0	34.0	41.0
15	38.72172772626876	40.0	38.0	41.0	35.0	41.0
16	38.65871915953006	40.0	38.0	41.0	35.0	41.0
17	38.664236077469944	40.0	38.0	41.0	35.0	41.0
18	38.484351414614146	40.0	38.0	41.0	35.0	41.0
19	38.255026517433265	40.0	37.0	41.0	34.0	41.0
20	38.24051880652594	40.0	36.0	41.0	34.0	41.0
21	38.107177609934666	40.0	36.0	41.0	34.0	41.0
22	38.17718728455024	40.0	36.0	41.0	34.0	41.0
23	38.18435408250524	40.0	36.0	41.0	34.0	41.0
24	38.141211475301645	40.0	36.0	41.0	34.0	41.0
25	37.87581809476068	40.0	35.0	41.0	34.0	41.0
26	37.932610475263736	40.0	35.0	41.0	34.0	41.0
27	37.90672350678838	40.0	35.0	41.0	34.0	41.0
28	37.832227562684906	40.0	35.0	41.0	34.0	41.0
29	37.78208665591085	40.0	35.0	41.0	34.0	41.0
30	37.55735053140178	40.0	35.0	41.0	33.0	41.0
31	37.41425327834669	40.0	35.0	41.0	33.0	41.0
32	37.175010847083506	40.0	35.0	41.0	33.0	41.0
33	36.87541650694424	40.0	35.0	41.0	31.0	41.0
34	36.57561996874355	40.0	35.0	41.0	30.0	41.0
35	36.43714659219038	40.0	35.0	41.0	30.0	41.0
36	36.2976032508955	40.0	35.0	41.0	29.0	41.0
37	36.23261904059828	40.0	35.0	41.0	29.0	41.0
38	36.11160350083477	40.0	35.0	41.0	28.0	41.0
39	36.02480015389519	40.0	35.0	41.0	27.0	41.0
40	35.953754214214804	39.0	35.0	41.0	27.0	41.0
41	35.83604685940074	39.0	35.0	41.0	26.0	41.0
42	35.80592917732068	39.0	35.0	41.0	26.0	41.0
43	35.737148136758904	39.0	35.0	41.0	26.0	41.0
44	35.65953778084819	39.0	35.0	41.0	26.0	41.0
45	35.60999223503278	39.0	35.0	41.0	25.0	41.0
46	35.49533048851894	39.0	35.0	41.0	25.0	41.0
47	35.438012954717465	39.0	35.0	41.0	24.0	41.0
48	35.37410432577477	39.0	35.0	41.0	24.0	41.0
49	35.33027087519465	39.0	35.0	41.0	24.0	41.0
50	35.22997221180809	39.0	35.0	41.0	24.0	41.0
51	34.33384304094651	37.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	21.0
9	25.0
10	34.0
11	22.0
12	15.0
13	25.0
14	35.0
15	46.0
16	69.0
17	155.0
18	250.0
19	435.0
20	782.0
21	1227.0
22	1931.0
23	3287.0
24	5142.0
25	8622.0
26	11723.0
27	12226.0
28	11239.0
29	10769.0
30	10896.0
31	12638.0
32	15590.0
33	22276.0
34	37635.0
35	48992.0
36	50772.0
37	77328.0
38	132543.0
39	235266.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45537671324243	18.43919946417514	21.997885345274252	18.107538477308182
2	37.18703180266593	22.16933245152512	23.81303419253468	16.830601553274274
3	30.566449444165954	22.30427157446295	31.262347772240734	15.866931209130367
4	26.401590624749886	24.61522691817859	30.555497049172043	18.427685407899485
5	21.313781904115995	35.51538741288985	26.923233540165096	16.247597142829058
6	23.61027446982685	33.05095812393899	29.26845022206683	14.07031718416733
7	76.503040693764	5.5677482858799765	14.052344023151678	3.8768669972043313
8	78.5202471871301	4.838992772823457	13.28104266800342	3.3597173720430287
9	73.19288992983446	6.816461730506491	14.930641852471238	5.060006487187804
10	36.13560188325028	29.062039701027697	21.711016845625995	13.091341570096029
11	26.485278155729013	24.991259146302937	31.233000970269863	17.290461727698187
12	23.366794304192943	23.73622701225685	34.242522533148545	18.654456150401657
13	22.421658782346423	24.207882073597286	35.7949543158755	17.575504828180794
14	20.103682672609043	25.80988046443771	35.5490870897942	18.53734977315905
15	19.235775577001654	25.22097861053424	37.16498659735766	18.37825921510644
16	23.22904687484642	25.422615010678584	34.29012332677594	17.058214787699054
17	23.100847687289463	24.608908228759024	33.38963987682768	18.90060420712383
18	24.04289407208642	23.2979907971799	34.06279092299202	18.596324207741656
19	23.23873553195642	26.042408235077712	31.837067678780294	18.88178855418557
20	24.24087967389946	25.731247884994236	32.16493745199551	17.862934989110794
21	23.01435184990164	25.63127217684467	33.85736330919594	17.497012664057753
22	23.495274322390767	23.290548785196854	33.175506513164635	20.03867037924774
23	21.04025285990904	26.1505280318125	33.48680727856855	19.322411829709914
24	20.408524333272954	25.54491675477728	34.36805382961724	19.678505082332524
25	21.425131253220776	26.586658017082925	32.47314908034986	19.51506164934644
26	20.7651792471773	26.11823250811249	32.69991982285203	20.416668421858173
27	21.00950190473382	26.056730597762062	33.29036624528029	19.64340125222383
28	19.313565664522525	26.568965686708147	33.536654717322904	20.580813931446432
29	20.415264268653825	25.8764373263238	33.10880923595811	20.59948916906426
30	21.359276467936862	25.13490401910772	32.99956611665986	20.506253396295563
31	22.11611504508034	25.726754594740324	31.900394988296384	20.256735371882957
32	22.50267842223729	25.74978270729163	31.26894729230117	20.47859157816991
33	22.190675580231208	26.911438653248577	30.205301240007692	20.69258452651252
34	20.723335481687734	27.747752301758137	31.38591325422334	20.142998962330783
35	21.132224894793822	28.3026736481164	29.973054300008567	20.592047157081215
36	22.16132877826034	29.671723022355522	28.50037841928857	19.666569780095568
37	21.598122927996428	29.212564924533783	28.72026881108944	20.469043336380345
38	21.777854538152948	29.287406290325524	28.931313037702918	20.003426133818607
39	21.240765937489908	28.17152573883031	28.618608119094656	21.96910020458512
40	22.829003626927726	27.026860046645968	29.944128743999	20.200007582427304
41	20.6004720763073	27.545975486293354	29.791637706006828	22.061914731392513
42	21.96643231349686	27.54190344200075	29.53945179050596	20.95221245399643
43	21.990022087329905	27.073337517709884	29.419817937495523	21.516822457464688
44	21.747805659579907	26.925058939330754	29.63310880923596	21.694026591853383
45	21.390729499714254	27.040199502087276	29.622577660203348	21.946493337995122
46	21.175051567526427	27.44529770154162	28.903651219577263	22.475999511354686
47	20.4712899815073	28.512875382807266	30.03076499670726	20.98506963897817
48	21.113268826535126	27.96736186291814	29.453938860361177	21.465430450185558
49	22.023721764234253	26.767091703841622	29.907059099404215	21.302127432519907
50	20.151845127518172	27.658869403922925	30.32788381474726	21.861401653811644
51	21.02171803761165	26.551694602294667	29.219866521196398	23.20672083889729
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	454.0
1	797.0
2	1140.0
3	8735.5
4	16331.0
5	10940.5
6	5550.0
7	5401.5
8	5253.0
9	5196.5
10	5140.0
11	5075.5
12	5011.0
13	4953.5
14	4896.0
15	4664.5
16	4433.0
17	4306.5
18	4180.0
19	4244.5
20	4309.0
21	4415.5
22	4522.0
23	5079.0
24	5636.0
25	5924.5
26	6907.0
27	7601.0
28	8761.5
29	9922.0
30	12056.0
31	14190.0
32	16578.0
33	18966.0
34	21092.0
35	23218.0
36	24133.5
37	25049.0
38	28002.5
39	30956.0
40	33116.5
41	35277.0
42	37608.0
43	39939.0
44	42603.0
45	45267.0
46	48976.5
47	52686.0
48	54689.0
49	56692.0
50	55510.0
51	54328.0
52	51085.5
53	47843.0
54	44070.5
55	40298.0
56	36325.5
57	32353.0
58	29080.0
59	25807.0
60	23695.0
61	21583.0
62	19410.0
63	17237.0
64	15064.5
65	12892.0
66	11294.0
67	9696.0
68	8105.0
69	6514.0
70	5338.5
71	4163.0
72	3556.0
73	2949.0
74	2424.5
75	1406.5
76	913.0
77	701.0
78	489.0
79	318.0
80	147.0
81	139.5
82	132.0
83	96.5
84	61.0
85	38.5
86	16.0
87	13.0
88	10.0
89	7.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	712173.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.81525007398142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17556846362818	25.824409654409447
2	8.738966725349258	6.084986238624738
3	3.769147648203719	3.9367145381391433
4	2.1462020426409936	2.9888224329534374
5	1.4095507330189623	2.4536930631009497
6	1.0665754343863696	2.227985428255609
7	0.8202476955771842	1.9989990050888657
8	0.6998063761199593	1.9491147190386466
9	0.6147289590340457	1.9261748192839718
>10	6.433605337816814	45.43174342712307
>50	0.10220439706518883	2.1649725038300325
>100	0.02216480888779159	1.532343237976343
>500	4.1045942384799246E-4	0.07673862900245232
>1k	4.1045942384799246E-4	0.14618923178679463
>5k	4.1045942384799246E-4	1.257113071386542
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8797	1.2352335738647773	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1023	0.143644872804782	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.40415320434782E-4	0.0	0.0	0.05813194265999975	0.0
2	1.40415320434782E-4	0.0	0.0	0.22915780294956423	0.0
3	1.40415320434782E-4	0.0	0.0	0.38768669972043307	0.0
4	1.40415320434782E-4	0.0	0.0	0.5296465886799977	0.0
5	1.40415320434782E-4	0.0	0.0	0.9483650742165176	0.0
6	1.40415320434782E-4	0.0	0.0	1.464672207455211	0.0
7	1.40415320434782E-4	0.0	0.0	1.8927985194608614	0.0
8	1.40415320434782E-4	0.0	0.0	2.5211570784065107	0.0
9	1.40415320434782E-4	0.0	0.0	2.881743621283031	0.0
10	1.40415320434782E-4	0.0	0.0	3.4695221526230284	0.0
11	1.40415320434782E-4	0.0	0.0	4.100548602656938	0.0
12	1.40415320434782E-4	0.0	0.0	4.612362445641719	0.0
13	1.40415320434782E-4	0.0	0.0	4.862301716015631	0.0
14	2.80830640869564E-4	0.0	0.0	4.983339722230413	0.0
15	2.80830640869564E-4	0.0	0.0	5.134847852979543	0.0
16	2.80830640869564E-4	0.0	0.0	5.405989836739107	0.0
17	2.80830640869564E-4	0.0	0.0	5.739897468733019	0.0
18	2.80830640869564E-4	0.0	0.0	6.1045560559021474	0.0
19	2.80830640869564E-4	0.0	0.0	6.345087499806929	0.0
20	2.80830640869564E-4	0.0	0.0	6.5794406696125805	0.0
21	2.80830640869564E-4	0.0	0.0	6.8904606043756225	0.0
22	2.80830640869564E-4	0.0	0.0	7.213135010734751	0.0
23	2.80830640869564E-4	0.0	0.0	7.533984017928228	0.0
24	2.80830640869564E-4	0.0	0.0	7.79712232842301	0.0
25	2.80830640869564E-4	0.0	0.0	8.041023740018225	0.0
26	2.80830640869564E-4	0.0	0.0	8.281133937961703	0.0
27	2.80830640869564E-4	0.0	0.0	8.53795355903692	0.0
28	2.80830640869564E-4	0.0	0.0	8.789858643896919	0.0
29	2.80830640869564E-4	0.0	0.0	9.073918837136482	0.0
30	2.80830640869564E-4	0.0	0.0	9.408388130412133	0.0
31	2.80830640869564E-4	0.0	0.0	9.704664456529523	0.0
32	2.80830640869564E-4	0.0	0.0	10.017088544496913	0.0
33	2.80830640869564E-4	0.0	0.0	10.294408802355607	0.0
34	2.80830640869564E-4	0.0	0.0	10.571729060214302	0.0
35	2.80830640869564E-4	0.0	0.0	10.868988293574736	0.0
36	2.80830640869564E-4	0.0	0.0	11.156418454504735	0.0
37	4.21245961304346E-4	0.0	0.0	11.45929430068256	0.0
38	4.21245961304346E-4	0.0	0.0	11.78702365857734	0.0
39	4.21245961304346E-4	0.0	0.0	12.206444220716033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGTCG	20	7.031478E-4	45.0	15
TTTCGCA	40	6.8084773E-9	45.0	14
TTCGTAG	20	7.031478E-4	45.0	1
ATATCGA	20	7.031478E-4	45.0	39
GTTCGTC	20	7.031478E-4	45.0	39
CCAACGG	20	7.031478E-4	45.0	2
TTCGCGG	40	6.8084773E-9	45.0	2
CTTACGG	35	1.211065E-7	45.0	2
TTTCGCG	25	3.889307E-5	44.999996	1
CGTTATT	640	0.0	43.59375	1
CGTTTTT	5020	0.0	43.476093	1
CATACGG	60	3.6379788E-12	41.250004	2
ATAACGG	50	1.0804797E-9	40.499996	2
TCGCAAC	45	1.9264917E-8	40.000004	16
TAGACGG	110	0.0	38.863636	2
TTAACGG	35	6.2449417E-6	38.571426	2
TTCGATG	35	6.2449417E-6	38.571426	1
TAACGGG	225	0.0	38.0	3
CGTAAGG	90	0.0	37.500004	2
CCGAATT	30	1.1394857E-4	37.500004	10
>>END_MODULE