##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935996.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813392
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11702106733285	33.0	31.0	34.0	30.0	34.0
2	32.249617650530126	34.0	31.0	34.0	30.0	34.0
3	32.13939404370832	33.0	31.0	34.0	30.0	34.0
4	35.80502758817397	37.0	35.0	37.0	35.0	37.0
5	32.012938410999865	37.0	35.0	37.0	0.0	37.0
6	33.846665568385234	37.0	35.0	37.0	17.0	37.0
7	35.533448816806654	37.0	35.0	37.0	32.0	37.0
8	35.782534128685796	37.0	35.0	37.0	35.0	37.0
9	37.76507637154042	39.0	38.0	39.0	35.0	39.0
10	37.437073391427504	39.0	37.0	39.0	34.0	39.0
11	37.34867443987647	39.0	37.0	39.0	34.0	39.0
12	37.39218974369062	39.0	37.0	39.0	35.0	39.0
13	37.36917009264906	39.0	37.0	39.0	34.0	39.0
14	38.69475726341051	40.0	38.0	41.0	35.0	41.0
15	38.7839713201015	40.0	38.0	41.0	35.0	41.0
16	38.74099941971399	40.0	38.0	41.0	35.0	41.0
17	38.67797322816059	40.0	38.0	41.0	35.0	41.0
18	38.51593450636348	40.0	38.0	41.0	35.0	41.0
19	38.31247418219014	40.0	37.0	41.0	34.0	41.0
20	38.197637793339496	40.0	37.0	41.0	34.0	41.0
21	38.17897274622814	40.0	37.0	41.0	34.0	41.0
22	38.22862408285304	40.0	37.0	41.0	34.0	41.0
23	38.18877982571749	40.0	37.0	41.0	34.0	41.0
24	38.17562011920452	40.0	36.0	41.0	34.0	41.0
25	37.952564077345244	40.0	36.0	41.0	34.0	41.0
26	37.95360539567638	40.0	36.0	41.0	34.0	41.0
27	37.90435607923363	40.0	36.0	41.0	34.0	41.0
28	37.818004111178865	40.0	36.0	41.0	33.0	41.0
29	37.76928221570903	40.0	36.0	41.0	33.0	41.0
30	37.591189733855266	40.0	36.0	41.0	33.0	41.0
31	37.49401518578988	40.0	36.0	41.0	33.0	41.0
32	37.22239338473946	40.0	36.0	41.0	32.0	41.0
33	36.94091040974094	40.0	35.0	41.0	31.0	41.0
34	36.651437437299606	40.0	35.0	41.0	30.0	41.0
35	36.52167343667014	40.0	35.0	41.0	30.0	41.0
36	36.39367734130653	40.0	35.0	41.0	29.0	41.0
37	36.295306568050826	40.0	35.0	41.0	29.0	41.0
38	36.166842063851135	40.0	35.0	41.0	27.0	41.0
39	36.055697621810886	40.0	35.0	41.0	26.0	41.0
40	35.96053194523674	40.0	35.0	41.0	26.0	41.0
41	35.863619263528534	39.0	35.0	41.0	25.0	41.0
42	35.816399718708816	40.0	35.0	41.0	25.0	41.0
43	35.75085936424258	39.0	35.0	41.0	25.0	41.0
44	35.741049825914196	39.0	35.0	41.0	24.0	41.0
45	35.71491359639633	39.0	35.0	41.0	25.0	41.0
46	35.56023540924917	39.0	35.0	41.0	24.0	41.0
47	35.41620891279973	39.0	35.0	41.0	23.0	41.0
48	35.368929126423666	39.0	35.0	41.0	23.0	41.0
49	35.34837692035329	39.0	35.0	41.0	24.0	41.0
50	35.243759466530285	39.0	35.0	41.0	24.0	41.0
51	34.28022404941283	38.0	34.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	16.0
10	31.0
11	19.0
12	9.0
13	15.0
14	18.0
15	35.0
16	38.0
17	130.0
18	277.0
19	569.0
20	1204.0
21	2247.0
22	3418.0
23	4585.0
24	6639.0
25	9713.0
26	12841.0
27	13529.0
28	12663.0
29	12059.0
30	12331.0
31	14504.0
32	17684.0
33	24268.0
34	38465.0
35	52356.0
36	55369.0
37	86703.0
38	150318.0
39	281091.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.742736589491905	18.15496095363613	22.00648641737317	17.09581603949879
2	34.71364360603497	25.896738595904555	23.515721816786986	15.873895981273481
3	28.794849223990397	25.79592619548754	30.55033735271554	14.85888722780652
4	25.537010445148216	23.797135944292545	33.220513602297544	17.445340008261702
5	23.339668941912386	35.62181580345024	25.181954088557546	15.856561166079825
6	23.17000904852765	34.589226350886165	28.497329700808464	13.743434899777721
7	75.93706355607137	4.6789247988669675	15.806523791726498	3.5774878533351693
8	77.48834510297617	6.678698585675788	12.531473139642385	3.301483171705647
9	71.6352017231544	7.109856010386135	13.808840018097055	7.446102248362413
10	34.658442669709075	33.925831579361486	19.75652575879773	11.659199992131715
11	25.55594350571434	24.42782815665755	33.49541180636151	16.520816531266597
12	23.08825265062848	22.70307551586443	34.634100163266915	19.57457167024018
13	22.425841414717627	23.7483279894565	36.63252158860673	17.193309007219153
14	18.229217892479888	28.677316718138364	33.80043078859886	19.293034600782892
15	17.520949308574462	26.12786946515333	37.37214037020281	18.9790408560694
16	20.451762495819974	25.970380824989675	34.17921494187304	19.398641737317305
17	20.485325648641737	26.016484056887702	33.519016661093296	19.979173633377265
18	21.55455180282078	24.655885477113127	33.23354544918071	20.55601727088538
19	21.043482109487186	26.49460530715817	31.786641619293032	20.67527096406161
20	24.23788284123768	25.259038692291046	33.00130810236639	17.501770364104885
21	21.992839860731355	27.531497728032733	32.8725878789071	17.603074532328815
22	21.68474732970081	23.48683045813089	33.767728229439186	21.060693982729113
23	21.0461868324252	26.74073509451777	33.31567067293506	18.897407400121956
24	19.896064874009088	26.07107028345496	33.71744497118241	20.31541987135354
25	19.464784507347012	29.339236186242303	31.71176997069064	19.484209335720045
26	20.531551822491494	27.23385526289907	32.29267069260578	19.941922222003658
27	21.639258807561422	27.2970474260873	31.802747014969412	19.26094675138187
28	18.788480830890887	28.165263489190945	33.65646576312528	19.38978991679289
29	20.212763341660605	26.690820662116177	33.11896355017015	19.97745244605307
30	20.16813541318331	28.11387375336861	32.526014516985654	19.19197631646242
31	22.463461651946417	27.137714656647717	29.825717489230286	20.57310620217558
32	22.636563919979544	28.831485925605367	30.345393119184845	18.186557035230248
33	21.729867025985012	27.604402305407476	30.12594173535024	20.53978893325727
34	21.99333162853827	27.366263744910203	30.40710997895234	20.23329464759919
35	22.48362413202982	27.783774612978736	29.365176938056926	20.367424316934514
36	21.68155083895588	29.505822530833843	28.876728563841297	19.935898066368985
37	21.01840195133466	29.3821429273954	29.89382733048764	19.705627790782305
38	21.478573676652832	27.366878454668843	29.452096897928676	21.702450970749652
39	22.683650687491394	27.669807423726812	29.32337667446938	20.32316521431241
40	22.601279579833587	26.26937565159234	31.124107441430453	20.005237327143615
41	20.887960540551173	27.27184432598304	29.849814111768985	21.990381021696795
42	22.322816059169504	27.717140075142122	29.376979365422823	20.583064500265554
43	21.505375022129552	27.765702146074712	28.9739756476582	21.75494718413754
44	21.770437870055275	27.116322757047033	29.574547081849833	21.53869229104786
45	21.360057635186973	26.583738222161024	29.267192202529653	22.789011940122354
46	22.641481598048667	28.2877136731121	28.39946790723292	20.67133682160631
47	20.991354721954483	27.494492200562583	31.192094340736077	20.322058736746857
48	21.542134665696246	26.821507956803114	29.63023978598265	22.00611759151799
49	20.867552176564313	26.4316590278734	30.995756043826344	21.70503275173594
50	20.183749041052774	27.063457717803963	30.77163286582607	21.98116037531719
51	20.368161968644884	26.285112221413538	29.5971684009678	23.74955740897378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	463.0
1	823.5
2	1184.0
3	9467.0
4	17750.0
5	11917.5
6	6085.0
7	5934.5
8	5784.0
9	5726.0
10	5668.0
11	5538.0
12	5408.0
13	5158.0
14	4908.0
15	4791.0
16	4674.0
17	4622.0
18	4570.0
19	4536.0
20	4502.0
21	4730.0
22	4958.0
23	5215.5
24	5473.0
25	6336.5
26	8482.5
27	9765.0
28	11264.0
29	12763.0
30	15091.0
31	17419.0
32	19705.5
33	21992.0
34	24905.5
35	27819.0
36	29603.5
37	31388.0
38	34141.5
39	36895.0
40	39399.0
41	41903.0
42	46165.5
43	50428.0
44	53074.5
45	55721.0
46	67157.0
47	78593.0
48	70855.0
49	63117.0
50	61792.0
51	60467.0
52	54711.5
53	48956.0
54	45282.0
55	41608.0
56	37090.5
57	32573.0
58	29933.5
59	27294.0
60	25584.5
61	23875.0
62	21776.5
63	19678.0
64	15989.5
65	12301.0
66	9766.0
67	7231.0
68	6001.0
69	4771.0
70	3932.5
71	3094.0
72	2585.0
73	2076.0
74	1710.5
75	1142.0
76	939.0
77	655.0
78	371.0
79	313.0
80	255.0
81	177.5
82	100.0
83	53.5
84	7.0
85	7.0
86	7.0
87	8.5
88	10.0
89	6.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	813392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.540611294325934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.97951283465386	24.07338570896068
2	8.513247419470323	5.540525502588142
3	3.575007129446923	3.489987521213288
4	2.0279393766195386	2.639615479321362
5	1.4257873363591007	2.3197995750416918
6	1.0623500573210714	2.0741712164273918
7	0.8968589759507763	2.0429037525569
8	0.7723445852719512	2.0106051947689543
9	0.645500717465134	1.8904489143517291
>10	6.939167479596044	45.62991699765379
>50	0.12652005999268395	2.552064292718011
>100	0.029995636263454414	1.6961203480426557
>500	0.002691916074925391	0.5539858920455091
>1k	0.0023073566356503353	1.6006400598947608
>5k	7.691188785501117E-4	1.885829544415139
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9463	1.163399689202746	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	5607	0.6893355233393001	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	3959	0.4867271868914373	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	3616	0.44455809744870856	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	1592	0.19572358715109076	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1530	0.18810118614395027	Illumina Single End Adapter 2 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.13228554005940554	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	1018	0.12515490685917738	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	841	0.10339418140330862	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4588390345614417E-4	0.0	0.0	0.06823278320908	0.0
2	2.4588390345614417E-4	0.0	0.0	0.37571060448098825	0.0
3	2.4588390345614417E-4	0.0	0.0	0.5226262367960344	0.0
4	2.4588390345614417E-4	0.0	0.0	0.8775596514349785	0.0
5	2.4588390345614417E-4	0.0	0.0	2.016493892243838	0.0
6	2.4588390345614417E-4	0.0	0.0	2.654562621712532	0.0
7	2.4588390345614417E-4	0.0	0.0	3.148789267659382	0.0
8	2.4588390345614417E-4	0.0	0.0	3.7931280366662077	0.0
9	2.4588390345614417E-4	0.0	0.0	4.087205185199756	0.0
10	2.4588390345614417E-4	0.0	0.0	5.171430257489623	0.0
11	2.4588390345614417E-4	0.0	0.0	5.779132324881484	0.0
12	2.4588390345614417E-4	0.0	0.0	6.776683321203061	0.0
13	2.4588390345614417E-4	0.0	0.0	7.047524440860004	0.0
14	2.4588390345614417E-4	0.0	0.0	7.184727658988532	0.0
15	2.4588390345614417E-4	0.0	0.0	7.401720203788579	0.0
16	3.6882585518421623E-4	0.0	0.0	7.696166178177312	0.0
17	3.6882585518421623E-4	0.0	0.0	8.073843853885949	0.0
18	3.6882585518421623E-4	0.0	0.0	8.460004524263823	0.0
19	3.6882585518421623E-4	0.0	0.0	8.811864390109566	0.0
20	3.6882585518421623E-4	0.0	0.0	9.071886618014439	0.0
21	3.6882585518421623E-4	0.0	0.0	9.374938529024137	0.0
22	3.6882585518421623E-4	0.0	0.0	9.724708381690501	0.0
23	3.6882585518421623E-4	0.0	0.0	10.059602258197769	0.0
24	7.376517103684325E-4	0.0	0.0	10.322698034895843	0.0
25	7.376517103684325E-4	0.0	0.0	10.566000157365698	0.0
26	7.376517103684325E-4	0.0	0.0	10.812498770580483	0.0
27	7.376517103684325E-4	0.0	0.0	11.078422212168302	0.0
28	7.376517103684325E-4	0.0	0.0	11.344960363514764	0.0
29	8.605936620965046E-4	0.0	0.0	11.616416192930346	0.0
30	8.605936620965046E-4	0.0	0.0	11.951555953341071	0.0
31	8.605936620965046E-4	0.0	0.0	12.249444302378189	0.0
32	8.605936620965046E-4	0.0	0.0	12.533071385014852	0.0
33	8.605936620965046E-4	0.0	0.0	12.817313177410155	0.0
34	9.835356138245767E-4	0.0	0.0	13.097989653205342	0.0
35	9.835356138245767E-4	0.0	0.0	13.384690284635207	0.0
36	9.835356138245767E-4	0.0	0.0	13.660572024313	0.0
37	9.835356138245767E-4	0.0	0.0	13.953173869425813	0.0
38	9.835356138245767E-4	0.0	0.0	14.23704683596593	0.0
39	9.835356138245767E-4	0.0	0.0	14.537639907941067	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATC	20	7.032068E-4	45.000004	9
GGACGTA	20	7.032068E-4	45.000004	8
TCGACGA	20	7.032068E-4	45.000004	30
CTAACCG	25	3.8897968E-5	45.0	2
GTTCACG	25	3.8897968E-5	45.0	1
GCGTCCA	30	2.1647174E-6	44.999996	28
TAGTACG	30	2.1647174E-6	44.999996	1
CGTTATT	685	0.0	44.014595	1
CTCGAAT	140	0.0	43.392857	43
CGCTCGA	130	0.0	43.26923	41
CGTTTTT	5545	0.0	43.214607	1
AGTACGG	155	0.0	42.09677	2
CCGCTCG	135	0.0	41.666668	40
GAGTACG	60	3.6379788E-12	41.249996	1
GCTAACG	55	6.002665E-11	40.909092	1
TAATACG	200	0.0	40.5	4
GCTCGAA	145	0.0	40.344826	42
CCGATGA	960	0.0	40.07812	18
TTTGGCG	90	0.0	40.0	1
ACCGCTC	170	0.0	39.705883	39
>>END_MODULE