##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935995.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	827693
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19406833209898	33.0	31.0	34.0	30.0	34.0
2	32.316613768631605	34.0	31.0	34.0	30.0	34.0
3	32.24343083727904	34.0	31.0	34.0	30.0	34.0
4	35.87130614853575	37.0	35.0	37.0	35.0	37.0
5	32.12995518869919	37.0	35.0	37.0	0.0	37.0
6	33.94067607192522	37.0	35.0	37.0	17.0	37.0
7	35.60938657207443	37.0	35.0	37.0	32.0	37.0
8	35.86998198607455	37.0	35.0	37.0	35.0	37.0
9	37.836011661328534	39.0	38.0	39.0	35.0	39.0
10	37.403264253775255	39.0	37.0	39.0	34.0	39.0
11	37.363052484435656	39.0	37.0	39.0	34.0	39.0
12	37.33868958659793	39.0	37.0	39.0	35.0	39.0
13	37.296489157211674	39.0	37.0	39.0	34.0	39.0
14	38.5648144903968	40.0	38.0	41.0	34.0	41.0
15	38.688669591261494	40.0	38.0	41.0	35.0	41.0
16	38.638377997639225	40.0	38.0	41.0	35.0	41.0
17	38.64007186239343	40.0	38.0	41.0	35.0	41.0
18	38.5451683172384	40.0	38.0	41.0	35.0	41.0
19	38.36647162655719	40.0	37.0	41.0	34.0	41.0
20	38.395690189478465	40.0	37.0	41.0	34.0	41.0
21	38.32264740670756	40.0	37.0	41.0	34.0	41.0
22	38.371017998219145	40.0	37.0	41.0	34.0	41.0
23	38.39680896177689	40.0	37.0	41.0	35.0	41.0
24	38.371734447434015	40.0	37.0	41.0	34.0	41.0
25	38.101850565366625	40.0	36.0	41.0	34.0	41.0
26	38.13925694671817	40.0	37.0	41.0	34.0	41.0
27	38.13037805079903	40.0	37.0	41.0	34.0	41.0
28	38.039966509321694	40.0	36.0	41.0	34.0	41.0
29	38.014701102945175	40.0	36.0	41.0	34.0	41.0
30	37.86436275285644	40.0	36.0	41.0	34.0	41.0
31	37.81045991690156	40.0	36.0	41.0	34.0	41.0
32	37.695093470646725	40.0	36.0	41.0	33.0	41.0
33	37.591493464364206	40.0	36.0	41.0	33.0	41.0
34	37.45512285352178	40.0	36.0	41.0	33.0	41.0
35	37.39075236832981	40.0	36.0	41.0	33.0	41.0
36	37.285320765066274	40.0	36.0	41.0	33.0	41.0
37	37.22211617109242	40.0	35.0	41.0	33.0	41.0
38	37.09908263087884	40.0	35.0	41.0	32.0	41.0
39	37.01463102865434	40.0	35.0	41.0	32.0	41.0
40	36.93387282482756	40.0	35.0	41.0	32.0	41.0
41	36.83008313468883	40.0	35.0	41.0	31.0	41.0
42	36.80970722236385	40.0	35.0	41.0	31.0	41.0
43	36.74651833469656	39.0	35.0	41.0	31.0	41.0
44	36.68678604265108	39.0	35.0	41.0	31.0	41.0
45	36.65985335142378	39.0	35.0	41.0	31.0	41.0
46	36.54691413362201	39.0	35.0	41.0	31.0	41.0
47	36.474212056885825	39.0	35.0	41.0	31.0	41.0
48	36.42417055599117	39.0	35.0	41.0	31.0	41.0
49	36.371399782286424	39.0	35.0	41.0	31.0	41.0
50	36.27975348347757	39.0	35.0	41.0	31.0	41.0
51	35.3216989874265	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	34.0
10	29.0
11	29.0
12	21.0
13	19.0
14	23.0
15	36.0
16	48.0
17	88.0
18	159.0
19	319.0
20	623.0
21	1057.0
22	1632.0
23	2491.0
24	3763.0
25	5583.0
26	7471.0
27	8338.0
28	8698.0
29	8935.0
30	10444.0
31	13247.0
32	17393.0
33	26210.0
34	46425.0
35	62644.0
36	60663.0
37	95403.0
38	158191.0
39	287435.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71970404485721	19.970810433336997	23.61950626621223	13.68997925559356
2	33.94555710873476	24.154970502348093	25.13903101753911	16.760441371378036
3	31.463477400437117	23.413391196977624	28.527364614657852	16.595766787927406
4	28.28186296126704	25.007218860132923	28.220366730176526	18.49055144842351
5	22.59098482166697	37.1706659353166	23.539404102728913	16.69894514028752
6	24.847860257365955	35.52621563792373	25.230248413360997	14.39567569134933
7	80.00804646167119	5.906054539545459	10.222993307905226	3.86290569087814
8	82.09142761869437	5.56691913547656	8.866572509372435	3.475080736456633
9	76.20506637122702	7.035096346109004	10.627370293091762	6.132466989572221
10	39.562736425220464	25.839411472611225	19.587576553142288	15.010275549026028
11	30.07093209680401	26.060991212925565	25.931353774889963	17.93672291538046
12	26.534959217970915	23.110621933494667	30.514695666146746	19.839723182387672
13	24.97894750831528	24.305146956661467	31.741962297615178	18.973943237408072
14	21.105651491555445	27.578703698110292	30.695922280362403	20.619722529971863
15	19.98724164635922	26.168277368541233	34.33616087124091	19.50832011385864
16	23.145417443424073	25.041168645862655	31.410317593600528	20.403096317112745
17	23.209571664856416	24.710852937018917	29.46756828920868	22.61200710891599
18	24.4606393916585	24.65612249952579	29.881731511562865	21.00150659725285
19	24.519235996921566	26.673899622202917	28.52241108720262	20.284453293672897
20	25.307209315531242	26.14604690386411	29.058721047538157	19.488022733066487
21	24.46486801265687	26.426464884927142	29.74859035898576	19.360076743430234
22	22.202918231759845	25.008547855303835	29.913748213407626	22.87478569952869
23	22.032202761168694	26.763183934139832	28.99505009707706	22.209563207614416
24	21.706598944294562	25.459318853729584	30.248292543249732	22.58578965872612
25	22.36493482486864	26.855851142875437	28.986351219594702	21.792862812661216
26	21.345716346519787	27.3346518576332	29.102577888178345	22.217053907668667
27	22.36759281521047	26.775507343906497	29.717540199083476	21.139359641799558
28	20.709731748365638	26.72718024678232	31.441126118017188	21.121961886834853
29	21.696087800670057	25.697088171580525	30.62862679761699	21.97819723013243
30	21.6422030873766	25.95769204282264	30.24237247385202	22.15773239594874
31	23.01010157147638	26.559122766533	28.413433483187607	22.01734217880301
32	22.16908926377292	26.867812099413673	29.082522142871813	21.880576493941597
33	21.748522701049787	26.603100424916004	28.485682493388246	23.162694380645966
34	20.76011274711759	26.229169510917693	29.63200123717369	23.37871650479103
35	21.506524762200478	26.024745890082436	29.63236369040212	22.83636565731497
36	22.388615102459486	27.12769106419892	28.56058949393072	21.923104339410866
37	21.398272064642327	27.800283438424632	29.36342339490608	21.438021102026962
38	20.729183404958118	28.269177098271943	27.511166579879255	23.490472916890685
39	22.425102060788237	26.126716065014442	27.838582662895544	23.609599211301774
40	22.360827021613087	25.856930045318737	29.582949233592647	22.199293699475533
41	20.59531734592415	27.23401067787211	28.96255012426105	23.20812185194269
42	21.144796440226028	27.872653266368086	28.942252743468895	22.040297549936994
43	21.346199617491028	26.520702724319285	29.34421337379922	22.788884284390466
44	21.756859125303706	25.829141964472335	29.048451539399274	23.36554737082469
45	21.69258408612855	25.952134426653362	28.162857484598756	24.19242400261933
46	21.313941280160638	27.629688785576295	28.578349702123855	22.47802023213921
47	20.90014051103489	26.69081410619638	30.21712156560464	22.191923817164096
48	21.060948926715582	26.325702887423237	29.51420393793351	23.099144247927676
49	20.916209270828677	25.909606581184086	30.392548928165397	22.78163521982184
50	20.122436700564098	25.917097281238334	30.171452458822294	23.789013559375274
51	20.97577241803422	25.319532725297904	28.252141796535675	25.4525530601322
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	560.0
1	1019.0
2	1478.0
3	5308.0
4	9138.0
5	6148.0
6	3158.0
7	3081.0
8	3004.0
9	3001.5
10	2999.0
11	2940.0
12	2881.0
13	2910.0
14	2939.0
15	2865.5
16	2792.0
17	2814.0
18	2836.0
19	2849.5
20	2863.0
21	2996.5
22	3130.0
23	3456.0
24	3782.0
25	4840.0
26	6951.0
27	8004.0
28	9450.0
29	10896.0
30	12209.0
31	13522.0
32	15328.5
33	17135.0
34	19318.0
35	21501.0
36	23821.0
37	26141.0
38	29500.5
39	32860.0
40	37108.0
41	41356.0
42	46196.5
43	51037.0
44	60141.5
45	69246.0
46	70746.0
47	72246.0
48	73283.5
49	74321.0
50	68095.5
51	61870.0
52	57390.5
53	52911.0
54	49006.5
55	45102.0
56	41911.0
57	38720.0
58	37116.5
59	35513.0
60	33612.0
61	31711.0
62	28249.0
63	24787.0
64	21424.5
65	18062.0
66	15150.5
67	12239.0
68	10188.5
69	8138.0
70	6966.5
71	5795.0
72	4586.5
73	3378.0
74	2547.0
75	1408.0
76	1100.0
77	798.0
78	496.0
79	368.0
80	240.0
81	168.5
82	97.0
83	71.0
84	45.0
85	38.0
86	31.0
87	20.0
88	9.0
89	7.0
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	827693.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.584842183722667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82271224240395	22.578560035082962
2	9.420585569294058	5.762542458302279
3	3.6496051274062475	3.348677905638754
4	2.064262688659106	2.5254059423354316
5	1.3630590829806597	2.0844473470026608
6	0.9947829400466277	1.8255167537031454
7	0.743774706749164	1.592376241831745
8	0.6104412620504504	1.4936199729796416
9	0.4851165978300342	1.3353493126802447
>10	6.4422631745312975	46.996094281813484
>50	0.36120871612319	6.600734552353129
>100	0.03736641856236508	1.9476308050531126
>500	0.002410736681442908	0.440917365671687
>1k	0.002410736681442908	1.4681270255517405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4726	0.5709846525221307	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2518	0.30421907639668333	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	1318	0.1592377850241575	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1226	0.14812255268559718	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	1120	0.13531587194769074	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	1039	0.12552963478004525	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.624532284313145E-4	0.0	0.0	0.07394045859998816	0.0
2	3.624532284313145E-4	0.0	0.0	0.37187701237052867	0.0
3	3.624532284313145E-4	0.0	0.0	0.5227783731407659	0.0
4	3.624532284313145E-4	0.0	0.0	0.7503990005956315	0.0
5	3.624532284313145E-4	0.0	0.0	1.4486047362971537	0.0
6	3.624532284313145E-4	0.0	0.0	2.0352956953846415	0.0
7	3.624532284313145E-4	0.0	0.0	2.524970006995347	0.0
8	3.624532284313145E-4	0.0	0.0	3.2698113914216984	0.0
9	3.624532284313145E-4	0.0	0.0	3.581521167872629	0.0
10	3.624532284313145E-4	0.0	0.0	4.414921957779032	0.0
11	3.624532284313145E-4	0.0	0.0	5.345339395162216	0.0
12	3.624532284313145E-4	0.0	0.0	6.181760628638879	0.0
13	3.624532284313145E-4	0.0	0.0	6.4879127889205295	0.0
14	3.624532284313145E-4	0.0	0.0	6.60933462044502	0.0
15	3.624532284313145E-4	0.0	0.0	6.830189454302501	0.0
16	3.624532284313145E-4	0.0	0.0	7.282651901127592	0.0
17	3.624532284313145E-4	0.0	0.0	7.8479581197376325	0.0
18	3.624532284313145E-4	0.0	0.0	8.477660195265637	0.0
19	3.624532284313145E-4	0.0	0.0	8.872613396512959	0.0
20	3.624532284313145E-4	0.0	0.0	9.248960665367473	0.0
21	3.624532284313145E-4	0.0	0.0	9.721841310727529	0.0
22	4.8327097124175266E-4	0.0	0.0	10.245827861296398	0.0
23	4.8327097124175266E-4	0.0	0.0	10.7863664426303	0.0
24	4.8327097124175266E-4	0.0	0.0	11.194247142358337	0.0
25	4.8327097124175266E-4	0.0	0.0	11.56709069667135	0.0
26	4.8327097124175266E-4	0.0	0.0	11.926885934760834	0.0
27	4.8327097124175266E-4	0.0	0.0	12.298762947131364	0.0
28	4.8327097124175266E-4	0.0	0.0	12.6672570627032	0.0
29	6.040887140521909E-4	0.0	0.0	13.057015101009673	0.0
30	6.040887140521909E-4	0.0	0.0	13.511773084948164	0.0
31	7.24906456862629E-4	0.0	0.0	13.934635184784696	0.0
32	7.24906456862629E-4	0.0	0.0	14.355322565250642	0.0
33	7.24906456862629E-4	0.0	0.0	14.760424456894041	0.0
34	7.24906456862629E-4	0.0	0.0	15.150907401657378	0.0
35	7.24906456862629E-4	0.0	0.0	15.549001864217772	0.0
36	7.24906456862629E-4	0.0	0.0	15.938397449295813	0.0
37	7.24906456862629E-4	0.0	0.0	16.338304177998364	0.0
38	7.24906456862629E-4	0.0	0.0	16.729995300189806	0.0
39	7.24906456862629E-4	0.0	0.0	17.159985646852153	1.2081774281043816E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGG	30	2.164763E-6	45.000004	2
TATGCGT	20	7.032141E-4	45.0	35
TGCGAAC	20	7.032141E-4	45.0	13
ACTAACG	20	7.032141E-4	45.0	1
TCGTAAG	40	6.8102963E-9	45.0	1
ACTCCGT	20	7.032141E-4	45.0	35
CGTAAGG	110	0.0	42.954544	2
CTAACGG	85	0.0	42.352943	2
CGTTATT	320	0.0	42.1875	1
CCCTCGT	370	0.0	41.959457	14
GCGTAAG	70	0.0	41.785713	1
CGCACGG	65	0.0	41.53846	2
ACGATAG	50	1.0804797E-9	40.5	1
CGTTTTT	2580	0.0	40.2907	1
TATAGCG	45	1.9272193E-8	40.000004	1
TCGTGTA	385	0.0	39.74026	17
GTAACGG	120	0.0	39.375004	2
TTAGGCG	40	3.456662E-7	39.375	1
CGGTTAC	40	3.456662E-7	39.375	29
AGTAACG	40	3.456662E-7	39.375	1
>>END_MODULE