##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935994.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775112
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.929494060213234	33.0	31.0	34.0	30.0	34.0
2	32.047693752644776	33.0	31.0	34.0	30.0	34.0
3	31.969232833448586	33.0	31.0	34.0	30.0	34.0
4	35.65881575823881	37.0	35.0	37.0	33.0	37.0
5	32.002216453880216	37.0	35.0	37.0	0.0	37.0
6	33.729073991887624	37.0	35.0	37.0	17.0	37.0
7	35.26329872328128	37.0	35.0	37.0	32.0	37.0
8	35.42136749269783	37.0	35.0	37.0	33.0	37.0
9	37.28222373024801	39.0	37.0	39.0	34.0	39.0
10	37.037513288402195	39.0	37.0	39.0	33.0	39.0
11	37.01925786208961	39.0	37.0	39.0	33.0	39.0
12	36.93963194996336	39.0	35.0	39.0	33.0	39.0
13	36.78705270980194	39.0	35.0	39.0	32.0	39.0
14	38.047487330863156	40.0	37.0	41.0	33.0	41.0
15	38.08543668527903	40.0	37.0	41.0	33.0	41.0
16	38.18781027774051	40.0	37.0	41.0	34.0	41.0
17	37.91693974548194	40.0	36.0	41.0	33.0	41.0
18	37.86280305297815	40.0	37.0	41.0	33.0	41.0
19	37.71368395793124	40.0	36.0	41.0	33.0	41.0
20	37.240843903848734	39.0	35.0	41.0	31.0	41.0
21	37.517250410263294	39.0	35.0	41.0	32.0	41.0
22	37.45791575927092	40.0	35.0	41.0	32.0	41.0
23	37.24630892051729	40.0	35.0	41.0	32.0	41.0
24	37.405631702257224	40.0	35.0	41.0	32.0	41.0
25	37.44180453921498	39.0	35.0	41.0	33.0	41.0
26	37.179515992527534	39.0	35.0	41.0	32.0	41.0
27	37.01102034286658	39.0	35.0	41.0	31.0	41.0
28	36.95670174116773	39.0	35.0	41.0	31.0	41.0
29	36.802773018608924	39.0	35.0	41.0	31.0	41.0
30	36.78428536779201	39.0	35.0	41.0	31.0	41.0
31	36.72527067056116	39.0	35.0	41.0	30.0	41.0
32	36.3207420346995	39.0	35.0	41.0	29.0	41.0
33	36.09376451403152	39.0	35.0	41.0	27.0	41.0
34	35.63170354735832	39.0	35.0	41.0	25.0	41.0
35	35.43373215741725	39.0	35.0	41.0	23.0	41.0
36	35.41066581345664	39.0	35.0	41.0	23.0	41.0
37	35.1033063608872	39.0	35.0	41.0	21.0	41.0
38	35.048562014263744	39.0	35.0	41.0	21.0	41.0
39	34.81418685299673	39.0	35.0	41.0	18.0	41.0
40	34.854698417777044	39.0	35.0	41.0	21.0	41.0
41	34.81252644779077	38.0	34.0	41.0	21.0	41.0
42	34.73381782245662	39.0	35.0	41.0	19.0	41.0
43	34.57237663718276	38.0	35.0	41.0	18.0	41.0
44	34.761741528966134	38.0	35.0	41.0	20.0	41.0
45	34.82314684845545	38.0	35.0	41.0	20.0	41.0
46	34.6377929899163	38.0	34.0	40.0	20.0	41.0
47	34.491272229045606	38.0	34.0	40.0	20.0	41.0
48	34.40591424207082	38.0	34.0	40.0	20.0	41.0
49	34.39076159316331	38.0	34.0	40.0	19.0	41.0
50	34.237959159450504	38.0	34.0	40.0	18.0	41.0
51	33.34935080349678	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	39.0
10	40.0
11	25.0
12	23.0
13	20.0
14	18.0
15	30.0
16	76.0
17	165.0
18	417.0
19	994.0
20	2503.0
21	5352.0
22	8538.0
23	10447.0
24	11175.0
25	12069.0
26	13219.0
27	12996.0
28	11882.0
29	11371.0
30	12750.0
31	15580.0
32	20468.0
33	28689.0
34	45031.0
35	59826.0
36	56008.0
37	80971.0
38	131580.0
39	222622.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.55298331079895	16.595537161081236	19.99465883640042	14.856820691719392
2	30.465274695785897	33.82440215091497	20.438078626056623	15.272244527242515
3	26.38534818194016	33.31712062256809	25.947863018505714	14.349668176986036
4	23.983630752717026	20.924976003467886	38.15693732002601	16.93445592378908
5	28.704238871285696	32.05446438708213	22.449916915232894	16.79137982639928
6	21.844972081454035	38.718275552436296	25.903224308229007	13.533528057880668
7	68.24665338686539	5.182734882184768	22.311356294316177	4.259255436633674
8	68.1482159997523	14.384759879862525	12.77854039158212	4.688483728803063
9	63.38967787880977	6.385141760158532	13.566555542940891	16.658624818090807
10	41.48368235816243	22.54667712537027	21.04895808605724	14.920682430410057
11	33.56908420976581	21.97708202169493	26.71446191002075	17.73937185851851
12	25.157138581263094	18.995061358874587	30.607189670654044	25.240610389208268
13	27.824495040716695	21.11927050542373	33.604562953482855	17.45167150037672
14	18.364055775165394	32.186832354550056	29.76666597859406	19.68244589169049
15	17.073016544705798	23.012158242937794	36.88344394100466	23.03138127135175
16	19.19916089545769	23.51234402254126	30.26233628172445	27.026158800276605
17	19.223931509252857	28.04949478268947	30.922111901247817	21.804461806809854
18	20.178245208434394	23.41906717996883	29.863555202344948	26.539132409251824
19	20.855824706623043	24.825315567298663	27.71522051006822	26.603639216010073
20	28.03504525797562	24.191600697705624	30.27755988811939	17.495794156199363
21	21.732472210467648	31.680711948724827	28.4086686827194	18.17814715808812
22	20.027299280620092	21.79801112613403	34.816903879697385	23.35778571354849
23	25.13146487217331	25.72763678023305	30.09268337995025	19.048214967643386
24	21.6030715561106	27.431390560331927	29.2023604330729	21.763177450484577
25	19.78785001393347	32.87679715963628	27.321858002456416	20.013494823973826
26	19.224060522866374	24.006079121468897	34.26924109031985	22.50061926534488
27	26.519264312770286	24.959876766196366	27.325728410861917	21.195130510171435
28	19.1398146332401	30.157190186708497	30.403348161297984	20.29964701875342
29	21.21951408312605	25.596687962513805	33.00452063701762	20.179277317342525
30	19.89106090474667	33.46690542786075	28.238241699264105	18.403791968128477
31	24.876146931024163	24.317517984497723	25.109790585102544	25.696544499375573
32	30.459211055950625	26.280073073310696	25.31801855731817	17.94269731342051
33	27.079183395431887	26.281492223059377	25.252608655265302	21.386715726243434
34	24.438661767589718	25.36059304977861	26.287684876508173	23.9130603061235
35	25.132109940240895	25.571014253424025	26.33361372292004	22.963262083415042
36	22.116158697065714	31.791534642735503	27.012870398084406	19.079436262114378
37	23.03073620328417	27.248320242752015	28.141739516353763	21.579204037610047
38	22.37470197855278	26.655889729484255	24.344739856949705	26.62466843501326
39	26.449984002311922	26.45966002332566	25.704930384254148	21.385425590108266
40	21.166231460743738	25.532826223823136	28.094391520193213	25.206550795239913
41	20.203273849456593	26.03959169771594	27.82720432660054	25.929930126226918
42	21.068310128084715	25.308600563531463	28.847314968675498	24.775774339708327
43	22.383603917885413	25.87316413627966	27.60723611555491	24.13599583028001
44	23.149944782173414	24.5120705136806	28.339001331420494	23.99898337272549
45	21.77401459401996	23.425904901485207	27.010806180268144	27.789274324226692
46	25.98501893919846	27.806175107597355	25.417746080566424	20.79105987263776
47	19.513954112437943	27.865005315360875	31.231357532846864	21.389683039354313
48	21.963535592275697	25.825945153732622	28.138514176015857	24.072005077975827
49	21.99811124069812	22.959004634169	32.46305050108887	22.57983362404401
50	20.53690305401026	25.40200641971741	30.192797944039057	23.868292582233277
51	20.793124090454025	24.01807738752593	28.378737524383574	26.81006099763647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	609.0
1	1065.0
2	1521.0
3	7772.5
4	14024.0
5	9175.0
6	4326.0
7	4150.5
8	3975.0
9	3867.5
10	3760.0
11	3714.5
12	3669.0
13	3504.5
14	3340.0
15	3328.0
16	3316.0
17	3211.5
18	3107.0
19	3036.0
20	2965.0
21	3195.5
22	3426.0
23	3550.5
24	3675.0
25	4034.0
26	5413.0
27	6433.0
28	7566.0
29	8699.0
30	9924.5
31	11150.0
32	12858.0
33	14566.0
34	15965.5
35	17365.0
36	18536.0
37	19707.0
38	22373.0
39	25039.0
40	27684.5
41	30330.0
42	33106.5
43	35883.0
44	42101.5
45	48320.0
46	75510.0
47	102700.0
48	81814.5
49	60929.0
50	59714.0
51	58499.0
52	55008.0
53	51517.0
54	49879.5
55	48242.0
56	44182.5
57	40123.0
58	37976.0
59	35829.0
60	33148.0
61	30467.0
62	27452.0
63	24437.0
64	20604.5
65	16772.0
66	14142.5
67	11513.0
68	9348.5
69	7184.0
70	6011.5
71	4839.0
72	4089.5
73	3340.0
74	2836.0
75	1885.5
76	1439.0
77	1057.5
78	676.0
79	546.0
80	416.0
81	282.0
82	148.0
83	120.5
84	93.0
85	50.0
86	7.0
87	8.0
88	9.0
89	4.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	775112.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.368136236940135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67337873015701	22.980594747869763
2	8.460008749109997	5.138293965173559
3	3.3392075910807484	3.042165331480946
4	1.8719892737440402	2.273953011965985
5	1.2904171145798304	1.9593781369019743
6	0.9154935710623865	1.6681100094039232
7	0.7246982244614695	1.540541408778017
8	0.5816991058126159	1.4132094155379018
9	0.4976986034614793	1.3602761095367744
>10	6.290040873873502	41.791559531761315
>50	0.29943854870734227	5.517702206198162
>100	0.04947619695542353	2.7593737103428726
>500	8.60455599224757E-4	0.2332141133031263
>1k	0.003872050196511407	2.64505306656683
>5k	8.60455599224757E-4	2.1501426681398037
>10k+	8.60455599224757E-4	3.5264325670390373
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	16301	2.1030509139324383	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	10690	1.3791555284913664	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	8801	1.1354488125587012	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7656	0.9877282250823106	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	4402	0.5679179266996254	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	3143	0.405489787282354	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT	2876	0.371043152473449	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT	2462	0.3176315164776187	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTC	2082	0.26860634334134936	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGAGCTT	1503	0.1939074611152969	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTA	1331	0.17171711959045918	No Hit
GAATATGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	1316	0.1697819153877117	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	1130	0.14578538327364304	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	903	0.11649929300539792	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	882	0.11379000712155146	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8704084054949476E-4	0.0	0.0	0.06295864339605114	0.0
2	3.8704084054949476E-4	0.0	0.0	0.596816976127321	0.0
3	3.8704084054949476E-4	0.0	0.0	0.7618253878149223	0.0
4	3.8704084054949476E-4	0.0	0.0	1.5041697199888533	0.0
5	3.8704084054949476E-4	0.0	0.0	4.064960934677827	0.0
6	3.8704084054949476E-4	0.0	0.0	4.95902527634716	0.0
7	3.8704084054949476E-4	0.0	0.0	5.659182156901196	0.0
8	3.8704084054949476E-4	0.0	0.0	6.433392851613703	0.0
9	3.8704084054949476E-4	0.0	0.0	6.647684463664606	0.0
10	3.8704084054949476E-4	0.0	0.0	8.650749827121757	0.0
11	3.8704084054949476E-4	0.0	0.0	9.274014594019961	0.0
12	3.8704084054949476E-4	0.0	0.0	11.088333040902477	0.0
13	3.8704084054949476E-4	0.0	0.0	11.431122212015811	0.0
14	3.8704084054949476E-4	0.0	0.0	11.612773379847042	0.0
15	3.8704084054949476E-4	0.0	0.0	11.977494865258182	0.0
16	3.8704084054949476E-4	0.0	0.0	12.348925058572181	0.0
17	3.8704084054949476E-4	0.0	0.0	12.75970440400871	0.0
18	3.8704084054949476E-4	0.0	0.0	13.160420687590955	0.0
19	3.8704084054949476E-4	0.0	0.0	13.666928134256727	0.0
20	3.8704084054949476E-4	0.0	0.0	13.975786725015224	0.0
21	3.8704084054949476E-4	0.0	0.0	14.286838547203502	0.0
22	3.8704084054949476E-4	0.0	0.0	14.689619048602008	0.0
23	3.8704084054949476E-4	0.0	0.0	15.03073104273963	0.0
24	3.8704084054949476E-4	0.0	0.0	15.31288381550021	0.0
25	3.8704084054949476E-4	0.0	0.0	15.560331926224855	0.0
26	3.8704084054949476E-4	0.0	0.0	15.803393574089938	0.0
27	5.160544540659931E-4	0.0	0.0	16.09548039509129	0.0
28	5.160544540659931E-4	0.0	0.0	16.339961192705054	0.0
29	6.450680675824913E-4	0.0	0.0	16.601729814530028	0.0
30	6.450680675824913E-4	0.0	0.0	16.905556874361384	0.0
31	6.450680675824913E-4	0.0	0.0	17.1754533538379	0.0
32	6.450680675824913E-4	0.0	0.0	17.4554128951687	0.0
33	6.450680675824913E-4	0.0	0.0	17.724406279350596	0.0
34	6.450680675824913E-4	0.0	0.0	17.991077418489198	0.0
35	9.030952946154878E-4	0.0	0.0	18.26110291157923	0.0
36	9.030952946154878E-4	0.0	0.0	18.53112840466926	0.0
37	0.0011611225216484843	0.0	0.0	18.78618831859138	0.0
38	0.0011611225216484843	0.0	0.0	19.066792927989763	0.0
39	0.0012901361351649826	0.0	0.0	19.35862172176408	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTCG	30	2.1645828E-6	45.000004	38
AGTCGGC	30	2.1645828E-6	45.000004	11
TCGATAG	20	7.031863E-4	45.0	1
CCGTCTA	40	6.8102963E-9	45.0	41
ACCTCGT	50	2.1827873E-11	45.0	39
GGACGTA	20	7.031863E-4	45.0	8
TTACGCG	20	7.031863E-4	45.0	1
ACGTACG	25	3.8896265E-5	45.0	1
CGTAAGC	130	0.0	44.999996	43
TAATACG	670	0.0	44.66418	4
CCGATGA	3745	0.0	44.3992	18
CGATGAA	3865	0.0	43.952133	19
GATGAAT	4090	0.0	43.899754	20
CGTTATT	525	0.0	43.714283	1
ATGAATG	4115	0.0	43.195625	21
ATAATAC	830	0.0	42.831326	3
AATACGG	700	0.0	42.75	5
ACCGATG	3910	0.0	42.640663	17
CACCGAT	4025	0.0	42.54037	16
CGTTTTT	3640	0.0	42.52747	1
>>END_MODULE