##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935993.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	908985
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13387569651864	33.0	31.0	34.0	30.0	34.0
2	32.253272606258626	34.0	31.0	34.0	30.0	34.0
3	32.153218149914466	34.0	31.0	34.0	30.0	34.0
4	35.80485266533551	37.0	35.0	37.0	35.0	37.0
5	32.167750842973206	37.0	35.0	37.0	0.0	37.0
6	33.92933106706931	37.0	35.0	37.0	17.0	37.0
7	35.547351166410884	37.0	35.0	37.0	32.0	37.0
8	35.80549514018383	37.0	35.0	37.0	35.0	37.0
9	37.77060457543304	39.0	38.0	39.0	35.0	39.0
10	37.35818962909179	39.0	37.0	39.0	34.0	39.0
11	37.300520910686096	39.0	37.0	39.0	34.0	39.0
12	37.305261362948784	39.0	37.0	39.0	34.0	39.0
13	37.3117829227105	39.0	37.0	39.0	34.0	39.0
14	38.60007480871521	40.0	38.0	41.0	35.0	41.0
15	38.723539992409115	40.0	38.0	41.0	35.0	41.0
16	38.63487516295648	40.0	38.0	41.0	35.0	41.0
17	38.61268337761349	40.0	38.0	41.0	35.0	41.0
18	38.49184529997745	40.0	38.0	41.0	34.0	41.0
19	38.333013196037335	40.0	37.0	41.0	34.0	41.0
20	38.37231087421684	40.0	37.0	41.0	34.0	41.0
21	38.28264162774963	40.0	37.0	41.0	34.0	41.0
22	38.32885251131757	40.0	37.0	41.0	34.0	41.0
23	38.346995825013614	40.0	37.0	41.0	34.0	41.0
24	38.317163649565174	40.0	37.0	41.0	34.0	41.0
25	38.024494353592196	40.0	36.0	41.0	34.0	41.0
26	38.09974091981716	40.0	37.0	41.0	34.0	41.0
27	38.07336534706293	40.0	37.0	41.0	34.0	41.0
28	37.95836124908552	40.0	36.0	41.0	34.0	41.0
29	37.87715198820663	40.0	36.0	41.0	33.0	41.0
30	37.71819556978388	40.0	36.0	41.0	33.0	41.0
31	37.684214811025484	40.0	36.0	41.0	33.0	41.0
32	37.58398763455943	40.0	36.0	41.0	33.0	41.0
33	37.438188748989255	40.0	36.0	41.0	33.0	41.0
34	37.28530833842142	40.0	36.0	41.0	32.0	41.0
35	37.21396062641298	40.0	36.0	41.0	32.0	41.0
36	37.09308404429116	40.0	36.0	41.0	32.0	41.0
37	37.045798335506085	40.0	35.0	41.0	32.0	41.0
38	36.912520008581	40.0	35.0	41.0	31.0	41.0
39	36.81870767944466	40.0	35.0	41.0	31.0	41.0
40	36.745160811234506	40.0	35.0	41.0	31.0	41.0
41	36.60854139507253	39.0	35.0	41.0	31.0	41.0
42	36.59787125200086	39.0	35.0	41.0	31.0	41.0
43	36.53620906835646	39.0	35.0	41.0	30.0	41.0
44	36.469780029373425	39.0	35.0	41.0	30.0	41.0
45	36.43950450227452	39.0	35.0	41.0	30.0	41.0
46	36.30833622116977	39.0	35.0	41.0	30.0	41.0
47	36.25578969949999	39.0	35.0	41.0	30.0	41.0
48	36.188508061189125	39.0	35.0	41.0	30.0	41.0
49	36.101320703862	39.0	35.0	41.0	30.0	41.0
50	35.97884233513204	39.0	35.0	41.0	29.0	41.0
51	35.02777933629268	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	9.0
8	14.0
9	39.0
10	38.0
11	28.0
12	24.0
13	16.0
14	42.0
15	60.0
16	75.0
17	141.0
18	254.0
19	477.0
20	784.0
21	1343.0
22	2016.0
23	3009.0
24	4622.0
25	6947.0
26	9340.0
27	10709.0
28	10980.0
29	11457.0
30	12792.0
31	15707.0
32	20086.0
33	28508.0
34	47794.0
35	64729.0
36	66748.0
37	104469.0
38	175972.0
39	309546.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73193177005121	19.4082410600835	23.49125673140921	15.36857043845608
2	34.808715215322586	23.31259591742438	24.353317161449308	17.525371705803728
3	31.194244129441078	23.63713372607909	28.647777466074796	16.520844678405034
4	28.30156713257094	25.26433329482885	27.75975401134232	18.674345561257887
5	22.711815926555442	36.68861422355704	23.592028471316908	17.007541378570604
6	24.756514133896598	34.27999361925664	26.13211439132659	14.831377855520167
7	79.23046034863061	6.119352904613388	10.515245026045534	4.134941720710462
8	81.08230608865932	5.516702695864068	9.705990747922133	3.6950004675544696
9	75.32918585015155	7.605296016985978	11.670159573590324	5.395358559272155
10	38.39986358410755	28.95988382646578	18.77918777537583	13.861064814050836
11	27.924113159183044	26.705391178072247	27.261946016710947	18.108549646033765
12	25.779523314466136	22.843941319163683	31.503050105337273	19.87348526103291
13	24.57620312766437	23.38168396618206	32.17104792708351	19.87106497907006
14	20.641374720154896	26.744995792009767	31.906577116234043	20.707052371601293
15	20.184931544524936	26.344329114341818	33.97855850206549	19.492180839067753
16	22.92810112378092	25.115375941297163	32.538930785436506	19.417592149485415
17	22.959894827747434	24.34836658470712	30.428224888199473	22.263513699345975
18	23.7728895416316	24.538248705974247	30.742311479287338	20.94655027310682
19	24.333404841664052	26.39460497147918	29.020170849904016	20.251819336952757
20	25.33738180498028	25.27676474309257	29.711601401563282	19.674252050363865
21	24.8636666171609	26.285582270334494	29.85989867819601	18.990852434308596
22	23.039324081255465	24.66311325269394	29.91105463786531	22.386508028185283
23	22.058009758136823	26.406486355660437	30.096646259289205	21.438857626913535
24	22.2087273167324	25.30536807538078	30.616786855668686	21.869117752218134
25	21.996072542451195	26.363251318778637	29.34437862010924	22.296297518660925
26	21.726431129226555	27.57108203105662	28.938541340066116	21.76394549965071
27	22.30289828765051	26.603739335632604	29.528100023652755	21.56526235306413
28	21.688256681903443	26.9188160420689	30.255284740672288	21.13764253535537
29	21.55547121239624	25.835409825244643	30.851884244514487	21.75723471784463
30	22.89949779149271	25.473027607716297	29.578925944872577	22.048548655918417
31	22.877495228194082	27.11298866317926	27.698806911005132	22.310709197621524
32	22.696194106613422	27.310791707233896	28.492769407635986	21.5002447785167
33	22.810937474215745	26.59933882297288	28.879244431976325	21.710479270835055
34	21.368669449990925	27.64292040022663	28.516642188814995	22.471767960967455
35	22.132378422086173	27.425865113285695	28.19496471338911	22.24679175123902
36	22.12489755056464	28.579899558298543	27.682855052613633	21.61234783852319
37	22.158451459595042	28.64678735072636	27.8863787631259	21.308382426552694
38	21.51509650874327	28.227638519887567	28.02444484782477	22.232820123544393
39	22.373636528655588	27.11056838121641	28.553606495156686	21.962188594971312
40	22.515002997849248	27.20561945466647	29.279031007112327	21.000346540371954
41	20.253469529200153	27.983740105722315	29.077597540113427	22.68519282496411
42	21.440947870426903	27.70749792350809	28.439413191636824	22.41214101442818
43	22.46318696128099	26.813533776684984	28.390127449847906	22.33315181218612
44	21.780227396491693	27.057872242116204	28.177472675566705	22.9844276858254
45	21.9240141476482	26.53894178671815	27.774165690302922	23.762878375330725
46	21.52532770067713	27.91102163402036	27.60287573502313	22.960774930279378
47	21.489903573766345	27.418054203314686	28.976825800205724	22.115216422713246
48	21.57439341683306	25.886345759280953	29.42765832219453	23.111602501691447
49	21.672524849144924	26.2262853622447	29.391574118384796	22.70961567022558
50	20.425639587011887	26.634982975516646	29.948679021105956	22.990698416365507
51	20.75886840816955	26.38382371546285	28.289575735573198	24.5677321407944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	484.0
1	989.0
2	1494.0
3	6966.5
4	12439.0
5	8224.5
6	4010.0
7	3902.0
8	3794.0
9	3801.0
10	3808.0
11	3821.0
12	3834.0
13	3788.0
14	3742.0
15	3644.0
16	3546.0
17	3574.5
18	3603.0
19	3618.0
20	3633.0
21	3907.5
22	4182.0
23	4608.5
24	5035.0
25	5932.5
26	7554.0
27	8278.0
28	10337.5
29	12397.0
30	14153.5
31	15910.0
32	18576.5
33	21243.0
34	24209.5
35	27176.0
36	28883.0
37	30590.0
38	33485.0
39	36380.0
40	39859.0
41	43338.0
42	48045.0
43	52752.0
44	59989.0
45	67226.0
46	69920.5
47	72615.0
48	72724.0
49	72833.0
50	68321.0
51	63809.0
52	60369.0
53	56929.0
54	53872.5
55	50816.0
56	48588.0
57	46360.0
58	43972.0
59	41584.0
60	39525.5
61	37467.0
62	34661.5
63	31856.0
64	26733.5
65	21611.0
66	17744.0
67	13877.0
68	11366.5
69	8856.0
70	7349.0
71	5842.0
72	4728.0
73	3614.0
74	3092.0
75	1952.5
76	1335.0
77	934.5
78	534.0
79	468.5
80	403.0
81	272.5
82	142.0
83	137.5
84	133.0
85	81.5
86	30.0
87	27.0
88	24.0
89	14.5
90	5.0
91	8.0
92	11.0
93	6.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	908985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.65322748984986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79158379698276	23.040885612723645
2	9.180715487054442	5.811985516626441
3	3.7748788338548325	3.5846129542387852
4	2.1316137210595687	2.6988981613273557
5	1.4294136352057996	2.262277748613122
6	1.0514656132346998	1.9969368152083462
7	0.8205404130811784	1.818092665191174
8	0.6629356118169334	1.6787241385571354
9	0.5930702304143958	1.6895328228688087
>10	7.38414137808984	49.59558187048083
>50	0.14406527419257736	2.8169119758750716
>100	0.032405865952367156	1.6531303165441134
>500	0.0028179013871614646	0.6059731078718166
>1k	0.0	0.0
>5k	3.522376733951831E-4	0.7464562938733785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6695	0.7365358064214481	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	979	0.1077025473467659	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.600768989587286E-4	0.0	0.0	0.06644774116184536	0.0
2	6.600768989587286E-4	0.0	0.0	0.28493319471718453	0.0
3	6.600768989587286E-4	0.0	0.0	0.44181147103637575	0.0
4	6.600768989587286E-4	0.0	0.0	0.6445650918331985	0.0
5	6.600768989587286E-4	0.0	0.0	1.2071706353790217	0.0
6	6.600768989587286E-4	0.0	0.0	1.8045402289366712	0.0
7	6.600768989587286E-4	0.0	0.0	2.234800354241269	0.0
8	6.600768989587286E-4	0.0	0.0	2.9124793038388974	0.0
9	6.600768989587286E-4	0.0	0.0	3.2421877148687823	0.0
10	6.600768989587286E-4	0.0	0.0	3.8818022299597903	0.0
11	6.600768989587286E-4	0.0	0.0	4.665203496207308	0.0
12	6.600768989587286E-4	0.0	0.0	5.368185393598354	0.0
13	6.600768989587286E-4	0.0	0.0	5.709995214442483	0.0
14	6.600768989587286E-4	0.0	0.0	5.886785810546929	0.0
15	6.600768989587286E-4	0.0	0.0	6.08700913656441	0.0
16	6.600768989587286E-4	0.0	0.0	6.435639752031111	0.0
17	6.600768989587286E-4	0.0	0.0	6.851378185558618	1.1001281649312145E-4
18	6.600768989587286E-4	0.0	0.0	7.328723796322271	1.1001281649312145E-4
19	6.600768989587286E-4	0.0	0.0	7.634889464622629	1.1001281649312145E-4
20	6.600768989587286E-4	0.0	0.0	7.929063735925236	1.1001281649312145E-4
21	6.600768989587286E-4	0.0	0.0	8.282424902501141	1.1001281649312145E-4
22	6.600768989587286E-4	0.0	0.0	8.663069247567341	1.1001281649312145E-4
23	6.600768989587286E-4	0.0	0.0	9.059445425392058	1.1001281649312145E-4
24	7.700897154518502E-4	0.0	0.0	9.365721106508909	1.1001281649312145E-4
25	7.700897154518502E-4	0.0	0.0	9.667376249333048	1.1001281649312145E-4
26	7.700897154518502E-4	0.0	0.0	9.949889162087382	1.1001281649312145E-4
27	7.700897154518502E-4	0.0	0.0	10.2278915493655	1.1001281649312145E-4
28	7.700897154518502E-4	0.0	0.0	10.537247589344158	1.1001281649312145E-4
29	8.801025319449716E-4	0.0	0.0	10.837362552737394	1.1001281649312145E-4
30	8.801025319449716E-4	0.0	0.0	11.219437064418004	1.1001281649312145E-4
31	9.90115348438093E-4	0.0	0.0	11.549035462631396	1.1001281649312145E-4
32	0.0011001281649312144	0.0	0.0	11.8864447708158	1.1001281649312145E-4
33	0.0012101409814243359	0.0	0.0	12.23782570669483	1.1001281649312145E-4
34	0.0012101409814243359	0.0	0.0	12.608018834194183	1.1001281649312145E-4
35	0.0012101409814243359	0.0	0.0	12.943337898865218	1.1001281649312145E-4
36	0.0012101409814243359	0.0	0.0	13.266775579354995	1.1001281649312145E-4
37	0.0012101409814243359	0.0	0.0	13.610565630896	1.1001281649312145E-4
38	0.0013201537979174573	0.0	0.0	13.945334631484569	1.1001281649312145E-4
39	0.0013201537979174573	0.0	0.0	14.288574618943107	1.1001281649312145E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	30	2.1649976E-6	45.000004	1
CGTTCGG	20	7.032507E-4	45.0	2
GTATACG	25	3.89016E-5	45.0	1
CCGAACA	20	7.032507E-4	45.0	11
CGCAATA	20	7.032507E-4	45.0	30
TATCGAC	20	7.032507E-4	45.0	14
ATCGCGA	20	7.032507E-4	45.0	4
CGTTATT	445	0.0	42.977528	1
CGTTTTT	3205	0.0	41.911076	1
GCGTATG	65	0.0	41.538464	1
TAAACGG	90	0.0	40.0	2
TTACGGG	335	0.0	39.626865	3
CGTATGG	120	0.0	39.375004	2
ACTTGCG	35	6.246846E-6	38.57143	43
ACGGGCG	100	0.0	38.25	5
CGTAAGG	190	0.0	37.894737	2
CACGGTC	30	1.1397338E-4	37.500004	37
CGCATGG	120	0.0	37.500004	2
CGAATAT	90	0.0	37.5	14
TACGGGA	475	0.0	37.42105	4
>>END_MODULE