##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935992.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	827155
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05089372608519	33.0	31.0	34.0	30.0	34.0
2	32.1604233789314	33.0	31.0	34.0	30.0	34.0
3	32.07468733187855	33.0	31.0	34.0	30.0	34.0
4	35.72418591436913	37.0	35.0	37.0	33.0	37.0
5	32.090075016169884	37.0	35.0	37.0	0.0	37.0
6	33.86436157672988	37.0	35.0	37.0	17.0	37.0
7	35.48886967980608	37.0	35.0	37.0	32.0	37.0
8	35.765521576971665	37.0	35.0	37.0	35.0	37.0
9	37.70254063627736	39.0	37.0	39.0	35.0	39.0
10	37.269867195386595	39.0	37.0	39.0	34.0	39.0
11	37.22826918775804	39.0	37.0	39.0	34.0	39.0
12	37.1982844811432	39.0	37.0	39.0	34.0	39.0
13	37.18363063754677	39.0	37.0	39.0	34.0	39.0
14	38.43798562542692	40.0	38.0	41.0	34.0	41.0
15	38.57234738350128	40.0	38.0	41.0	34.0	41.0
16	38.506389975276704	40.0	38.0	41.0	34.0	41.0
17	38.46902333903561	40.0	38.0	41.0	34.0	41.0
18	38.351778082705174	40.0	38.0	41.0	34.0	41.0
19	38.2116918836252	40.0	37.0	41.0	34.0	41.0
20	38.229168656418686	40.0	37.0	41.0	34.0	41.0
21	38.15590306532633	40.0	37.0	41.0	34.0	41.0
22	38.21903512642733	40.0	37.0	41.0	34.0	41.0
23	38.21715760649455	40.0	37.0	41.0	34.0	41.0
24	38.204213236938664	40.0	37.0	41.0	34.0	41.0
25	37.90780567124662	40.0	36.0	41.0	34.0	41.0
26	37.97064879013002	40.0	36.0	41.0	34.0	41.0
27	37.94262018605944	40.0	36.0	41.0	34.0	41.0
28	37.78988218653094	40.0	36.0	41.0	33.0	41.0
29	37.69309379741403	40.0	36.0	41.0	33.0	41.0
30	37.52660142294975	40.0	36.0	41.0	33.0	41.0
31	37.4911159335312	40.0	36.0	41.0	33.0	41.0
32	37.3976570292146	40.0	36.0	41.0	33.0	41.0
33	37.24538327157546	40.0	35.0	41.0	32.0	41.0
34	37.093201395143595	40.0	35.0	41.0	32.0	41.0
35	37.05614667142192	40.0	35.0	41.0	32.0	41.0
36	36.92645272046956	40.0	35.0	41.0	31.0	41.0
37	36.86676983153097	40.0	35.0	41.0	31.0	41.0
38	36.73990243666544	40.0	35.0	41.0	31.0	41.0
39	36.63374700025993	40.0	35.0	41.0	30.0	41.0
40	36.60591303927317	40.0	35.0	41.0	30.0	41.0
41	36.450159885390285	39.0	35.0	41.0	30.0	41.0
42	36.4256070506737	39.0	35.0	41.0	30.0	41.0
43	36.31758618396794	39.0	35.0	41.0	30.0	41.0
44	36.27198409004358	39.0	35.0	41.0	30.0	41.0
45	36.24010977386342	39.0	35.0	41.0	30.0	41.0
46	36.097151078092985	39.0	35.0	41.0	29.0	41.0
47	36.02156790444354	39.0	35.0	41.0	29.0	41.0
48	35.95874654689871	39.0	35.0	41.0	29.0	41.0
49	35.84003602710496	39.0	35.0	41.0	28.0	41.0
50	35.68054838573182	38.0	35.0	41.0	28.0	41.0
51	34.65737739601405	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	36.0
10	32.0
11	25.0
12	22.0
13	20.0
14	20.0
15	46.0
16	58.0
17	116.0
18	209.0
19	444.0
20	864.0
21	1425.0
22	2290.0
23	3302.0
24	4881.0
25	6902.0
26	8992.0
27	9927.0
28	10404.0
29	11154.0
30	12609.0
31	15383.0
32	19399.0
33	27939.0
34	47024.0
35	62172.0
36	61279.0
37	93762.0
38	157162.0
39	269068.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89462071800328	18.95424678566895	23.489793327731803	14.66133916859597
2	33.45515653051726	23.854416645006076	25.42111212529695	17.26931469917972
3	30.39031378641246	24.1087825135555	28.873911177469765	16.626992522562276
4	27.110396479499006	25.11476083684436	29.47923907852821	18.29560360512842
5	22.38395463969873	36.821756502711104	24.26751938874818	16.526769468841994
6	24.23705351475842	34.73436054911111	26.364587048376663	14.663998887753808
7	78.50608410757354	5.781141382207688	11.356759011309851	4.356015498908911
8	80.62346234986187	5.519038148835466	10.045759259147319	3.8117402421553397
9	74.65807496781134	7.590838476464508	11.620071207935636	6.131015347788503
10	37.896524835127636	29.556733623081527	19.39503478791762	13.151706753873215
11	27.72974835429877	26.204641209930422	28.285508761961182	17.780101673809625
12	24.78132877151199	22.82909490966022	32.90894693255799	19.480629386269804
13	24.354927431980705	23.3725238921363	33.697553662856414	18.57499501302658
14	20.07483482539548	27.462325682610878	32.33178787530753	20.13105161668611
15	19.131118109665056	25.63534041382812	35.91104448380292	19.322496992703908
16	22.234164092582407	25.418694198789826	32.03680084143842	20.310340867189343
17	21.936759132206177	24.79523184892795	30.226257472904113	23.041751545961763
18	23.542020540285677	24.400263553989276	31.529882549219916	20.52783335650513
19	23.56922221349082	26.239822040609074	29.45868670321766	20.732269042682447
20	23.693140946980918	25.58686098736029	30.453300771923036	20.26669729373576
21	23.719496345908567	26.89471743506356	30.588825552647325	18.796960666380546
22	22.417080232846324	24.99652423064601	30.430209573780004	22.156185962727662
23	20.622616075584382	26.062829820287615	30.812604650881635	22.501949453246368
24	20.850747441531514	25.170252250182855	31.41563552175832	22.563364786527313
25	20.71534355713258	27.28110209090195	29.650186482581866	22.35336786938361
26	20.445744751588276	26.452720469561324	30.133046406054486	22.96848837279591
27	21.832304707098427	25.73217837043843	30.29456389673036	22.14095302573278
28	20.206732716359085	26.775150969286287	31.8517085673181	21.166407747036526
29	21.944617393354328	25.069303818510434	30.89626490802812	22.089813880107116
30	22.63965036782707	25.682006395415613	30.315599857342335	21.362743379414983
31	22.585851503043568	27.29029021162902	28.50324304392768	21.620615241399737
32	23.239296141593776	26.89628908729319	28.510859512425117	21.353555258687912
33	22.14095302573278	26.767050915487424	28.60890643228899	22.483089626490802
34	21.449063355719304	26.406779865925976	29.92002708077688	22.22412969757784
35	21.92370232906771	26.362290018194894	29.264768997346323	22.449238655391067
36	22.169484558516846	28.83365270112615	28.55945983521831	20.4374029051387
37	21.36661206182638	28.88406646879968	28.744310316687926	21.005011152686016
38	20.523239296141593	28.01542637111545	27.731320006528403	23.730014326214555
39	21.675381276786094	26.306315019554983	28.755795467596762	23.262508236062164
40	22.48405679709365	24.537964468569978	30.684454546004076	22.293524188332295
41	20.479837515338723	26.284916369966936	29.773017149143755	23.46222896555059
42	21.82021507456281	26.76644643386064	29.41625209301763	21.997086398558917
43	21.47711130320194	26.639988877538066	29.0015776970459	22.881322122214094
44	21.08516541639717	26.381028948625108	28.443520259201723	24.090285375776002
45	21.408079501423554	25.33116525923195	28.375818317002256	24.884936922342245
46	21.96746679884665	26.852403721188896	28.214784411627807	22.96534506833665
47	20.744721364194135	25.917754229860186	30.329744727409008	23.00777967853667
48	20.70615543640551	25.951363408309202	29.90636579601163	23.436115359273654
49	21.436611034207615	24.899565377710346	30.74200119687362	22.92182239120842
50	20.348665002327255	25.29030230126155	30.32599694132297	24.035035755088224
51	20.40210117813469	24.832467917137656	29.102042543416896	25.66338836131076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	408.0
1	875.0
2	1342.0
3	6373.5
4	11405.0
5	7516.0
6	3627.0
7	3510.0
8	3393.0
9	3399.0
10	3405.0
11	3344.5
12	3284.0
13	3245.5
14	3207.0
15	3172.5
16	3138.0
17	3148.5
18	3159.0
19	3231.5
20	3304.0
21	3543.0
22	3782.0
23	4004.0
24	4226.0
25	5128.0
26	7214.0
27	8398.0
28	9619.5
29	10841.0
30	12829.5
31	14818.0
32	17463.0
33	20108.0
34	21895.5
35	23683.0
36	25834.5
37	27986.0
38	29909.5
39	31833.0
40	36047.0
41	40261.0
42	43735.5
43	47210.0
44	53009.0
45	58808.0
46	66596.5
47	74385.0
48	74415.0
49	74445.0
50	72699.5
51	70954.0
52	63382.0
53	55810.0
54	49851.5
55	43893.0
56	41028.5
57	38164.0
58	35565.5
59	32967.0
60	30572.0
61	28177.0
62	26206.0
63	24235.0
64	20530.5
65	16826.0
66	14000.0
67	11174.0
68	8895.0
69	6616.0
70	5593.0
71	4570.0
72	3815.0
73	3060.0
74	2470.5
75	1531.5
76	1182.0
77	929.5
78	677.0
79	483.5
80	290.0
81	201.0
82	112.0
83	77.0
84	42.0
85	31.0
86	20.0
87	14.5
88	9.0
89	6.5
90	4.0
91	3.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	827155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.93523683137112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67447889168966	25.34096126448712
2	9.359465449417902	6.352313532820637
3	3.676883633204912	3.74327750682603
4	1.985581103124348	2.6952465992967953
5	1.2910271739761832	2.1905656452308775
6	0.9775355131451717	1.9903739489794379
7	0.7247744531368298	1.7216774901568068
8	0.6080420172996575	1.6507239888390823
9	0.45483464175305754	1.3891429158301662
>10	6.045914305691427	45.360559611922824
>50	0.16312339772523413	3.3728072249596535
>100	0.03399910815608436	1.9970002021163402
>500	0.0021701558397500473	0.518090833013708
>1k	0.001808463199791706	0.9032528879762797
>5k	3.6169263995834114E-4	0.7740063475442887
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6306	0.7623722276961392	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	2011	0.24312251029129972	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1581	0.19113709038813764	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	1445	0.1746951901396957	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	1284	0.15523088175734898	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1038	0.12549038571972607	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	902	0.1090484854712841	TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC	886	0.10711414426558503	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.417926507123816E-4	0.0	0.0	0.11618136866729936	0.0
2	2.417926507123816E-4	0.0	0.0	0.5393185074139671	0.0
3	2.417926507123816E-4	0.0	0.0	0.7711976594471411	0.0
4	2.417926507123816E-4	0.0	0.0	1.0653384190387534	0.0
5	2.417926507123816E-4	1.208963253561908E-4	0.0	1.9640817017366756	0.0
6	2.417926507123816E-4	1.208963253561908E-4	0.0	2.7138807115957713	0.0
7	2.417926507123816E-4	1.208963253561908E-4	0.0	3.308086150721449	0.0
8	2.417926507123816E-4	1.208963253561908E-4	0.0	4.21130259745755	0.0
9	2.417926507123816E-4	1.208963253561908E-4	0.0	4.582937901602481	0.0
10	2.417926507123816E-4	1.208963253561908E-4	0.0	5.562198136987626	0.0
11	2.417926507123816E-4	1.208963253561908E-4	0.0	6.6327350980166955	0.0
12	2.417926507123816E-4	1.208963253561908E-4	0.0	7.6329103976884625	0.0
13	3.626889760685724E-4	1.208963253561908E-4	0.0	8.047705689985554	0.0
14	3.626889760685724E-4	1.208963253561908E-4	0.0	8.241018914230102	0.0
15	3.626889760685724E-4	1.208963253561908E-4	0.0	8.505298281458735	0.0
16	3.626889760685724E-4	1.208963253561908E-4	0.0	9.019349456873258	0.0
17	3.626889760685724E-4	1.208963253561908E-4	0.0	9.668925413012072	0.0
18	3.626889760685724E-4	1.208963253561908E-4	0.0	10.425494617091113	0.0
19	3.626889760685724E-4	1.208963253561908E-4	0.0	10.87692149597113	0.0
20	3.626889760685724E-4	1.208963253561908E-4	0.0	11.296189952306399	0.0
21	3.626889760685724E-4	1.208963253561908E-4	0.0	11.78461110674541	0.0
22	3.626889760685724E-4	1.208963253561908E-4	0.0	12.340492410733177	0.0
23	3.626889760685724E-4	1.208963253561908E-4	0.0	12.914387267199013	0.0
24	3.626889760685724E-4	1.208963253561908E-4	0.0	13.34260205161064	0.0
25	3.626889760685724E-4	1.208963253561908E-4	0.0	13.747846534204593	0.0
26	4.835853014247632E-4	1.208963253561908E-4	0.0	14.145232755650392	0.0
27	4.835853014247632E-4	1.208963253561908E-4	0.0	14.53089203353664	0.0
28	4.835853014247632E-4	1.208963253561908E-4	0.0	14.940851472819483	0.0
29	4.835853014247632E-4	1.208963253561908E-4	0.0	15.350085534150189	0.0
30	6.04481626780954E-4	1.208963253561908E-4	0.0	15.838143999613132	0.0
31	7.253779521371448E-4	1.208963253561908E-4	0.0	16.26889760685724	0.0
32	7.253779521371448E-4	1.208963253561908E-4	0.0	16.694210879460318	0.0
33	7.253779521371448E-4	1.208963253561908E-4	0.0	17.127503309536905	0.0
34	7.253779521371448E-4	1.208963253561908E-4	0.0	17.552212100513206	0.0
35	7.253779521371448E-4	1.208963253561908E-4	0.0	17.97728358046557	0.0
36	7.253779521371448E-4	1.208963253561908E-4	0.0	18.382528063059524	0.0
37	8.462742774933355E-4	1.208963253561908E-4	0.0	18.79490542884949	0.0
38	9.671706028495264E-4	1.208963253561908E-4	0.0	19.225538139768243	0.0
39	9.671706028495264E-4	1.208963253561908E-4	0.0	19.678053085576465	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGG	65	0.0	45.000004	2
TCGATAG	20	7.032138E-4	45.000004	1
GCGTAGC	20	7.032138E-4	45.000004	21
AAGTACG	25	3.8898546E-5	45.0	1
CGTATGG	50	2.1827873E-11	45.0	2
ACGCTAG	25	3.8898546E-5	45.0	1
GTACGAG	25	3.8898546E-5	45.0	1
TATCCGG	35	1.2113742E-7	45.0	2
CGAATAT	90	0.0	42.5	14
CGTTATT	465	0.0	42.096775	1
CGTAAGG	155	0.0	42.09677	2
CGTTTTT	3230	0.0	41.30805	1
CATACGG	50	1.0804797E-9	40.5	2
TATAGCG	50	1.0804797E-9	40.5	1
TACGGGA	490	0.0	39.94898	4
TTGTCGC	40	3.456662E-7	39.375004	38
ACGGGAG	430	0.0	38.720932	5
CTAACGG	70	0.0	38.571426	2
ATAGCGG	135	0.0	38.333332	2
GCTACGA	100	0.0	38.25	10
>>END_MODULE