##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935989.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	764681
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91049993395939	33.0	31.0	34.0	30.0	34.0
2	32.082464452497184	33.0	31.0	34.0	30.0	34.0
3	31.88333435772564	33.0	31.0	34.0	30.0	34.0
4	35.64938320685358	37.0	35.0	37.0	33.0	37.0
5	32.09030432297912	37.0	35.0	37.0	0.0	37.0
6	33.83382613141951	37.0	35.0	37.0	17.0	37.0
7	35.43914128898194	37.0	35.0	37.0	32.0	37.0
8	35.62356590525984	37.0	35.0	37.0	33.0	37.0
9	37.590663296198024	39.0	37.0	39.0	35.0	39.0
10	37.28562106290074	39.0	37.0	39.0	34.0	39.0
11	37.22077441442902	39.0	37.0	39.0	34.0	39.0
12	37.25360902127815	39.0	37.0	39.0	34.0	39.0
13	37.1759975728441	39.0	37.0	39.0	33.0	39.0
14	38.489717934668185	40.0	38.0	41.0	34.0	41.0
15	38.53746464211874	40.0	38.0	41.0	34.0	41.0
16	38.58752473253553	40.0	38.0	41.0	34.0	41.0
17	38.435531940770076	40.0	38.0	41.0	34.0	41.0
18	38.22800357273164	40.0	38.0	41.0	34.0	41.0
19	37.89557214053965	39.0	37.0	41.0	34.0	41.0
20	37.51242413503147	39.0	35.0	41.0	33.0	41.0
21	37.629165625927676	39.0	35.0	41.0	33.0	41.0
22	37.64257644691054	39.0	35.0	41.0	33.0	41.0
23	37.525105239962805	39.0	35.0	41.0	33.0	41.0
24	37.53350874416914	39.0	35.0	41.0	33.0	41.0
25	37.43670497893893	39.0	35.0	41.0	33.0	41.0
26	37.34033799715176	39.0	35.0	41.0	33.0	41.0
27	37.2474692061134	39.0	35.0	41.0	33.0	41.0
28	37.181382824995	39.0	35.0	41.0	33.0	41.0
29	37.115237595807926	39.0	35.0	41.0	33.0	41.0
30	36.87500408667144	39.0	35.0	41.0	32.0	41.0
31	36.67752696876214	39.0	35.0	41.0	31.0	41.0
32	36.11681341631347	39.0	35.0	41.0	29.0	41.0
33	35.63025627680039	39.0	35.0	41.0	24.0	41.0
34	35.060391195805835	39.0	35.0	41.0	20.0	41.0
35	34.84638561700892	39.0	35.0	41.0	18.0	41.0
36	34.545875992734224	39.0	35.0	41.0	15.0	41.0
37	34.312955336931346	39.0	35.0	41.0	12.0	41.0
38	34.29147317639643	39.0	35.0	41.0	15.0	41.0
39	34.126426575264716	39.0	34.0	41.0	12.0	41.0
40	34.1852968754291	39.0	34.0	41.0	15.0	41.0
41	34.04826718592459	39.0	34.0	41.0	15.0	41.0
42	33.88804089548452	39.0	34.0	41.0	11.0	41.0
43	33.77303607648157	39.0	34.0	41.0	10.0	41.0
44	33.78410605206616	38.0	34.0	41.0	11.0	41.0
45	33.85368539299394	38.0	34.0	41.0	11.0	41.0
46	33.73197058642754	38.0	34.0	41.0	11.0	41.0
47	33.510063673610304	38.0	33.0	40.0	10.0	41.0
48	33.557595912543924	38.0	33.0	40.0	10.0	41.0
49	33.534030530378026	38.0	33.0	40.0	10.0	41.0
50	33.39693806960027	38.0	33.0	40.0	10.0	41.0
51	32.56125757015017	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	15.0
10	19.0
11	10.0
12	11.0
13	14.0
14	21.0
15	26.0
16	70.0
17	133.0
18	404.0
19	842.0
20	1807.0
21	3758.0
22	6152.0
23	8548.0
24	11327.0
25	16185.0
26	20589.0
27	20374.0
28	17232.0
29	14803.0
30	14141.0
31	15623.0
32	18950.0
33	24715.0
34	37085.0
35	51187.0
36	52114.0
37	75316.0
38	128232.0
39	224792.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.08837541406155	16.185180487026614	20.096746224896396	22.629697874015438
2	37.61071610253165	27.762949517511228	20.767744981240543	13.858589398716589
3	24.209049263679887	27.600921168434944	35.26307048298571	12.926959084899456
4	21.436259041351885	20.481481820523854	43.21880627346567	14.863452864658596
5	23.87178444344766	31.106173685497613	30.492846036451805	14.529195834602927
6	19.27941193778844	33.90158772089276	34.131356735684555	12.687643605634245
7	66.44796980701757	3.7603915881262906	26.366811781644895	3.4248268232112475
8	67.37672310414408	9.479116128163247	19.592745210094144	3.551415557598528
9	62.75636507249428	5.507002266304512	20.372155186280292	11.364477474920914
10	33.21725006898301	27.90366178837973	27.138898442618558	11.740189700018702
11	24.367285181663988	20.919180677955907	38.27556850503674	16.437965635343367
12	19.757258255403233	19.11869132357153	40.059449626707085	21.064600794318153
13	21.332686440489564	21.412196719939423	41.93016434303978	15.324952496531234
14	16.48033624478704	28.53464385802707	37.08029884357006	17.90472105361582
15	14.483294341038944	23.384391661359444	43.10411792629868	19.028196071302936
16	17.19841345606861	23.95443328656002	37.49458924701934	21.35256401035203
17	17.546270928661755	25.832994412048947	38.53685392993941	18.083880729349886
18	18.32973488291196	22.442822562611077	38.47264414834421	20.754798406132753
19	17.175658869515523	24.948311779683294	36.3478365488354	21.528192801965787
20	21.107102177247768	24.04061301379268	39.21766069772886	15.634624111230696
21	17.877389395054934	28.87870889952804	37.78712953506103	15.456772170356004
22	17.164020029267107	21.436651361809698	40.79544280556206	20.60388580336114
23	18.316003666888545	25.888050049628536	38.25686789654771	17.539078386935206
24	17.088040633937553	25.294469196959255	38.33559353508195	19.281896634021244
25	15.70641875501026	30.63630455052499	35.87835973432058	17.778916960144166
26	16.636479786996148	24.124700365250344	40.047680012972734	19.191139834780778
27	20.785399401842074	23.921739915075698	36.01854891124534	19.27431177183688
28	15.82999969922098	26.235253654791997	39.21609141589761	18.71865523008941
29	18.433307483774282	21.69636750488112	40.83611335968855	19.03421165165605
30	19.144846020758983	26.27487802103099	37.35400774963678	17.22626820857325
31	20.878379350343476	23.486918074334266	34.595079516818124	21.039623058504134
32	22.443999523984512	25.929505244670654	34.291292708985836	17.335202522358998
33	21.405396498670687	23.28291143627212	33.00958177331462	22.30211029174257
34	19.400769732738226	23.542889126315416	36.34744422837758	20.70889691256877
35	23.386353263648502	24.32020672672657	32.74659629309477	19.54684371653016
36	23.2568875125706	26.118996025793763	32.29869710375961	18.32541935787603
37	21.129202896371165	26.261800672437264	33.71248926022747	18.896507170964103
38	20.633440611182962	25.88569612688167	30.50775421384865	22.97310904808672
39	19.959957158606006	27.168714797412253	33.4901743341341	19.381153709847638
40	21.6349039664906	23.832813944638353	32.847030330294594	21.685251758576452
41	20.960635872998022	24.75947486598987	32.10397538319901	22.175913877813098
42	22.071164315577345	24.38833971289989	33.33115377523438	20.20934219628839
43	22.651537046166965	24.809953431561656	30.93551428634947	21.602995235921906
44	21.033345931179145	24.462226732454447	32.486749376537404	22.017677959829
45	20.060914289749583	24.11881555838317	31.771026088002706	24.04924406386454
46	22.75576351445897	26.691130026769333	30.85848870313242	19.69461775563928
47	18.633521690744246	25.76420755844594	35.49715502281344	20.10511572799638
48	20.4542809354489	25.387579918946596	32.682386511499566	21.475752634104943
49	20.28401385675857	23.39079956217037	35.32518788880592	20.99999869226514
50	20.151278768532237	23.893754389085125	33.558699640765234	22.396267201617405
51	19.51571962687709	22.687892075257526	32.60902258588876	25.18736571197663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	421.0
1	667.0
2	913.0
3	15696.0
4	30479.0
5	20216.0
6	9953.0
7	9720.0
8	9487.0
9	9430.5
10	9374.0
11	9228.5
12	9083.0
13	8854.0
14	8625.0
15	8261.0
16	7897.0
17	7525.5
18	7154.0
19	6795.5
20	6437.0
21	5984.5
22	5532.0
23	5890.0
24	6248.0
25	6680.5
26	8250.0
27	9387.0
28	10146.0
29	10905.0
30	12562.0
31	14219.0
32	16019.5
33	17820.0
34	20700.0
35	23580.0
36	24728.0
37	25876.0
38	28022.0
39	30168.0
40	32171.0
41	34174.0
42	36559.0
43	38944.0
44	40995.0
45	43046.0
46	47808.0
47	52570.0
48	73054.0
49	93538.0
50	76975.5
51	60413.0
52	58385.0
53	56357.0
54	48656.5
55	40956.0
56	36624.0
57	32292.0
58	26260.0
59	20228.0
60	17259.0
61	14290.0
62	11976.5
63	9663.0
64	8102.0
65	6541.0
66	5321.5
67	4102.0
68	3392.5
69	2683.0
70	2255.0
71	1827.0
72	1371.0
73	915.0
74	861.0
75	609.5
76	412.0
77	307.0
78	202.0
79	114.0
80	26.0
81	17.5
82	9.0
83	8.0
84	7.0
85	6.5
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	764681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.32629365950114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74859985304437	29.798156695828254
2	8.71457729648611	6.679948971670964
3	3.3442864558277274	3.845223143626373
4	1.8706290725719117	2.8677711665356544
5	1.2243195330496126	2.3461814978361377
6	0.8747920290991779	2.0116521718947578
7	0.6668174045143561	1.78896477638785
8	0.5361527353032012	1.6438997743660217
9	0.44058534397196153	1.5197402947627547
>10	4.46317366708929	33.96509245841816
>50	0.08003399199497567	1.9859795536460916
>100	0.02771739772873176	1.9988598805055384
>500	0.003464674716091443	1.0528771668457015
>1k	0.003464674716091443	2.382219242428035
>5k	6.929349432182887E-4	2.3342827236701464
>10k+	6.929349432182887E-4	3.7791504815775845
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17651	2.308282800278809	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	10809	1.4135306094959859	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCG	9826	1.2849802728196464	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGC	7753	1.013886836471679	No Hit
GAACTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT	3369	0.44057587412267335	No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2944	0.38499714259933226	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCT	2153	0.2815553152229492	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1938	0.25343901574643546	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTC	1550	0.20269890320277345	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGACTGGT	1438	0.18805227277779885	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT	1259	0.16464381879502696	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGACTGGTCGTA	1255	0.16412072485127785	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT	1024	0.13391204959976774	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCG	1010	0.13208122079664592	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCC	980	0.1281580162185277	No Hit
GAATATGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	877	0.11468834716698859	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCGGTCGAATGC	869	0.1136421592794904	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	841	0.10998050167324676	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	781	0.10213409251701036	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.230939437490927E-4	0.0	0.0	0.09141066667015396	0.0
2	5.230939437490927E-4	0.0	0.0	0.6495519046504359	0.0
3	5.230939437490927E-4	0.0	0.0	0.8889981574015832	0.0
4	5.230939437490927E-4	0.0	0.0	1.553327465962931	0.0
5	5.230939437490927E-4	0.0	0.0	3.56475445316413	0.0
6	5.230939437490927E-4	0.0	0.0	4.476768744090673	0.0
7	5.230939437490927E-4	0.0	0.0	5.365636128006319	0.0
8	5.230939437490927E-4	0.0	0.0	6.371545781835825	0.0
9	5.230939437490927E-4	0.0	0.0	6.784920770883545	0.0
10	5.230939437490927E-4	0.0	0.0	8.930521354656387	0.0
11	5.230939437490927E-4	0.0	0.0	9.852474430514162	0.0
12	5.230939437490927E-4	0.0	0.0	11.710634892196877	0.0
13	5.230939437490927E-4	0.0	0.0	12.14114120790238	0.0
14	5.230939437490927E-4	0.0	0.0	12.360971437762936	0.0
15	5.230939437490927E-4	0.0	0.0	12.761138304730993	0.0
16	6.53867429686366E-4	0.0	0.0	13.180005780188079	0.0
17	6.53867429686366E-4	0.0	0.0	13.684529888934078	0.0
18	6.53867429686366E-4	0.0	0.0	14.203569854619115	0.0
19	7.846409156236392E-4	0.0	0.0	14.799243083063395	0.0
20	9.154144015609124E-4	0.0	0.0	15.188163430240845	0.0
21	9.154144015609124E-4	0.0	0.0	15.606507811754183	0.0
22	0.0010461878874981855	0.0	0.0	16.129470982017338	0.0
23	0.0011769613734354588	0.0	0.0	16.59267066920716	0.0
24	0.0011769613734354588	0.0	0.0	16.95046692673154	0.0
25	0.0011769613734354588	0.0	0.0	17.26693876269974	0.0
26	0.0011769613734354588	0.0	0.0	17.596749494233542	0.0
27	0.001307734859372732	0.0	0.0	17.96226138742822	0.0
28	0.001307734859372732	0.0	0.0	18.29756460537139	0.0
29	0.001307734859372732	0.0	0.0	18.67549997973011	0.0
30	0.001307734859372732	0.0	0.0	19.154392485232403	0.0
31	0.001307734859372732	0.0	0.0	19.541874324064544	0.0
32	0.001307734859372732	0.0	0.0	19.92190207419826	0.0
33	0.001307734859372732	0.0	0.0	20.289375569681997	0.0
34	0.001438508345310005	0.0	0.0	20.64089469988139	0.0
35	0.001438508345310005	0.0	0.0	21.006929687019817	0.0
36	0.0015692818312472783	0.0	0.0	21.33007097077082	0.0
37	0.0015692818312472783	0.0	0.0	21.686167172978013	0.0
38	0.0015692818312472783	0.0	0.0	22.055863817722685	0.0
39	0.0015692818312472783	0.0	0.0	22.479308365187574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	115	0.0	45.000004	33
ATTACGG	35	1.2112105E-7	45.000004	2
ACCCGCC	35	1.2112105E-7	45.000004	39
TCACGCC	20	7.031804E-4	45.0	13
GAGTACG	20	7.031804E-4	45.0	1
CCGTGGT	25	3.889577E-5	45.0	33
CGACCCA	25	3.889577E-5	45.0	34
ATATGCG	20	7.031804E-4	45.0	1
CCGTCGA	40	6.8102963E-9	45.0	41
CGCCTTG	20	7.031804E-4	45.0	41
ATATCCG	20	7.031804E-4	45.0	18
CTTTGCG	25	3.889577E-5	45.0	1
AGCGACG	20	7.031804E-4	45.0	1
CGGTCGA	285	0.0	45.0	41
CGATCGC	20	7.031804E-4	45.0	10
TCGACGG	20	7.031804E-4	45.0	2
TCGCGTC	20	7.031804E-4	45.0	1
TCGCGAG	20	7.031804E-4	45.0	1
TATCCGC	20	7.031804E-4	45.0	19
ACCGGTC	335	0.0	44.328354	39
>>END_MODULE