##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935988.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	457631
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.909092696954534	33.0	31.0	34.0	30.0	34.0
2	32.029233159466905	33.0	31.0	34.0	30.0	34.0
3	31.954933122974623	33.0	31.0	34.0	30.0	34.0
4	35.65215206137696	37.0	35.0	37.0	33.0	37.0
5	32.065681739217844	37.0	35.0	37.0	0.0	37.0
6	33.80848325397537	37.0	35.0	37.0	17.0	37.0
7	35.41823434164206	37.0	35.0	37.0	32.0	37.0
8	35.649752748393354	37.0	35.0	37.0	33.0	37.0
9	37.571720447259914	39.0	37.0	39.0	35.0	39.0
10	37.151626528797216	39.0	37.0	39.0	34.0	39.0
11	37.16196018189327	39.0	37.0	39.0	34.0	39.0
12	37.12380935732064	39.0	37.0	39.0	34.0	39.0
13	37.026029705155466	39.0	37.0	39.0	33.0	39.0
14	38.215033509530606	40.0	37.0	41.0	33.0	41.0
15	38.340708999171824	40.0	37.0	41.0	34.0	41.0
16	38.318492409823634	40.0	37.0	41.0	34.0	41.0
17	38.28547672688258	40.0	37.0	41.0	34.0	41.0
18	38.1774333469542	40.0	37.0	41.0	34.0	41.0
19	37.99548763086417	40.0	37.0	41.0	34.0	41.0
20	37.93151469196798	40.0	36.0	41.0	34.0	41.0
21	37.93672631443237	40.0	36.0	41.0	34.0	41.0
22	37.96481444657377	40.0	36.0	41.0	34.0	41.0
23	37.95723191829225	40.0	36.0	41.0	34.0	41.0
24	37.91265233343021	40.0	36.0	41.0	34.0	41.0
25	37.69332497142894	40.0	35.0	41.0	33.0	41.0
26	37.70266437369846	40.0	35.0	41.0	33.0	41.0
27	37.66673804877729	40.0	35.0	41.0	33.0	41.0
28	37.61423286446941	40.0	35.0	41.0	33.0	41.0
29	37.57003568377142	40.0	35.0	41.0	33.0	41.0
30	37.406493441222295	40.0	35.0	41.0	33.0	41.0
31	37.35560965057	40.0	35.0	41.0	33.0	41.0
32	37.157773402588546	40.0	35.0	41.0	32.0	41.0
33	36.983220105281326	40.0	35.0	41.0	32.0	41.0
34	36.816515926587144	40.0	35.0	41.0	31.0	41.0
35	36.710552825311225	40.0	35.0	41.0	31.0	41.0
36	36.50864124152429	40.0	35.0	41.0	30.0	41.0
37	36.45844796353394	39.0	35.0	41.0	30.0	41.0
38	36.32934831774946	39.0	35.0	41.0	30.0	41.0
39	36.23828805303837	39.0	35.0	41.0	30.0	41.0
40	36.17130395449609	39.0	35.0	41.0	29.0	41.0
41	36.075598025483416	39.0	35.0	41.0	29.0	41.0
42	36.02321521050803	39.0	35.0	41.0	29.0	41.0
43	35.93183809663244	39.0	35.0	41.0	29.0	41.0
44	35.90484910331686	39.0	35.0	41.0	29.0	41.0
45	35.896163065876216	39.0	35.0	41.0	29.0	41.0
46	35.773966361544566	39.0	35.0	41.0	28.0	41.0
47	35.645061195592085	39.0	35.0	41.0	27.0	41.0
48	35.62379296857075	38.0	35.0	41.0	27.0	41.0
49	35.6138439048054	38.0	35.0	41.0	27.0	41.0
50	35.482578758869046	38.0	35.0	40.0	27.0	41.0
51	34.5309561633718	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	12.0
9	16.0
10	13.0
11	14.0
12	15.0
13	8.0
14	16.0
15	20.0
16	23.0
17	44.0
18	133.0
19	271.0
20	540.0
21	946.0
22	1590.0
23	2254.0
24	3237.0
25	4572.0
26	5533.0
27	6180.0
28	6198.0
29	6521.0
30	7354.0
31	9257.0
32	11617.0
33	16770.0
34	27476.0
35	37832.0
36	34665.0
37	52032.0
38	84872.0
39	137492.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40534404356348	18.702622855532077	23.637166188479362	15.254866912425078
2	31.997832314681478	26.90071258284513	24.063055168902455	17.038399933570933
3	27.4511560624171	26.701643900872103	29.349847366109376	16.497352670601423
4	25.850958523351785	23.785320487466976	32.28627431271046	18.07744667647078
5	22.88000594365329	34.73387947931849	25.082435411936693	17.303679165091527
6	22.78713636095457	34.23915774936576	28.09862094132609	14.875084948353587
7	73.39734414845148	5.5577528620220225	15.975534874167177	5.0693681153593175
8	74.69511462291672	7.279882700254135	12.844409578896535	5.180593097932614
9	69.18325026058112	6.297650290299389	14.847988881872077	9.67111056724741
10	37.76142787529691	23.29737277413462	23.2464583911492	15.69474095941927
11	27.738505477120214	23.475463856250997	28.938817518918082	19.84721314771071
12	24.646276148250447	20.134781079078994	33.87598305184745	21.34295972082311
13	22.972657009686845	23.489885956152445	35.51551359064399	18.02194344351672
14	18.354744324575915	28.59050195463157	32.624319593733816	20.4304341270587
15	16.67850298603023	24.09146233537501	38.78015256833562	20.44988211025914
16	18.80357755484222	23.39810895677959	34.163988016546085	23.63432547183211
17	19.293710434826313	24.578973015377017	31.63575020048904	24.491566349307632
18	20.449663593593964	24.137787868391783	33.61638525362137	21.796163284392883
19	20.80628279115707	26.20670365425419	29.97043469520203	23.016578859386712
20	21.87024043388669	25.730774357506377	32.008758147940156	20.39022706066678
21	21.296852704471505	27.756423843664436	31.664157366961593	19.282566084902463
22	19.250225618456792	24.289875467352516	31.34293786915659	25.1169610450341
23	18.543542723285793	27.206854430753165	31.190631753530685	23.058971092430365
24	19.802198714684977	25.448887859432602	32.35576261223562	22.393150813646802
25	18.61150140615474	28.07829889146496	30.258002626570313	23.052197075809985
26	17.710775712309697	26.158629987916026	31.840500315756582	24.29009398401769
27	19.862727830938027	26.586704134990857	30.099578044319554	23.45098998975157
28	17.007589083781475	25.413269643009322	33.96295268458649	23.616188588622713
29	18.146279426000426	23.786850104123193	32.43508416169359	25.63178630818279
30	18.95916142044573	25.724000340886	31.634657617163175	23.6821806215051
31	20.487904009999323	25.177490161287153	29.383498932546093	24.951106896167435
32	21.882914400466753	27.322886779960275	27.840334243091046	22.953864576481926
33	20.59235497595224	23.871416053545325	30.244017559999214	25.292211410503224
34	18.702404338866906	24.610876448492345	30.099141010989207	26.587578201651553
35	20.70620215850762	24.085125352084976	28.672227187406445	26.536445302000956
36	20.41273427717965	23.887804803433337	31.734956766477794	23.96450415290922
37	18.427291857413504	26.126945071465872	31.561891567660403	23.88387150346021
38	18.83132917131925	26.900275549514785	30.539452091313745	23.728943187852224
39	19.026901586649505	26.042160605378566	31.08924002089019	23.84169778708173
40	21.557324569358283	23.071426542345254	31.332012035897915	24.03923685239855
41	19.6380926991397	23.38696460685574	29.63588568082145	27.33905701318311
42	20.876208124012578	23.848908837032457	31.19915390347245	24.07572913548252
43	20.808030924478455	23.896763986705448	30.572666624420116	24.72253846439599
44	19.83650583111721	24.21645386785423	30.5068931082029	25.440147192825663
45	19.517034466633596	24.200065117966222	28.7071898538342	27.57571056156598
46	22.287607264368017	25.342907276823468	29.270088783321057	23.099396675487455
47	18.666349089113282	23.87622342017914	33.459490288026814	23.997937202680763
48	19.599633766069168	23.79187598742218	30.788998122941848	25.819492123566807
49	19.87168701421014	21.981683933125158	34.270624149150734	23.876004903513966
50	19.571008082931446	22.121534598836178	32.28955206268806	26.017905255544317
51	18.930754253973177	22.119786465514792	29.65795586400397	29.291503416508057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	426.0
1	586.5
2	747.0
3	4038.5
4	7330.0
5	4834.5
6	2339.0
7	2303.0
8	2267.0
9	2249.0
10	2231.0
11	2258.5
12	2286.0
13	2182.5
14	2079.0
15	2117.5
16	2156.0
17	2003.5
18	1851.0
19	2003.0
20	2155.0
21	2023.5
22	1892.0
23	2069.0
24	2246.0
25	2508.5
26	3306.0
27	3841.0
28	4053.5
29	4266.0
30	5279.0
31	6292.0
32	7182.5
33	8073.0
34	9456.0
35	10839.0
36	11943.0
37	13047.0
38	14658.0
39	16269.0
40	18017.0
41	19765.0
42	22785.5
43	25806.0
44	30525.0
45	35244.0
46	44188.5
47	53133.0
48	51083.5
49	49034.0
50	47193.0
51	45352.0
52	38495.0
53	31638.0
54	28110.5
55	24583.0
56	21766.0
57	18949.0
58	17041.0
59	15133.0
60	13822.0
61	12511.0
62	11610.0
63	10709.0
64	8940.0
65	7171.0
66	5806.5
67	4442.0
68	3746.0
69	3050.0
70	2647.5
71	2245.0
72	1851.5
73	1458.0
74	1266.0
75	765.5
76	457.0
77	343.0
78	229.0
79	202.5
80	176.0
81	107.5
82	39.0
83	26.5
84	14.0
85	7.5
86	1.0
87	3.0
88	5.0
89	7.0
90	9.0
91	4.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	457631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.56206029327493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.87364310053896	30.41279703307221
2	9.822427053871523	7.7719090266312
3	3.6074009298259093	4.28148639263366
4	1.8430079145635905	2.9165276094779062
5	1.136470897113536	2.2480565076578976
6	0.8231366405652928	1.9538988842188731
7	0.6065566725752054	1.679764215619595
8	0.4733751833599929	1.4982158036342492
9	0.38400102100237543	1.36726843910176
>10	4.273521266799244	35.949510189860405
>50	0.12069718954792227	2.97157159288163
>100	0.029056730817092362	2.287813897837521
>500	0.0033526997096645036	0.9315728829343235
>1k	0.0033526997096645036	3.729607524438769
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4216	0.9212662603713472	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	3582	0.7827266946513676	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	3218	0.7031866285282247	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGC	2802	0.6122836958160613	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	2009	0.43899998033350013	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1044	0.2281313984411021	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	825	0.1802762487681123	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	814	0.17787256545120414	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT	739	0.16148381556319394	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	686	0.14990243230900005	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC	617	0.13482478241203066	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTC	533	0.11646938253745921	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTA	498	0.1088212992563878	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	489	0.10685464926982657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.555499955204084E-4	0.0	0.0	0.18967246537057147	0.0
2	6.555499955204084E-4	0.0	0.0	1.1456828755045003	0.0
3	6.555499955204084E-4	0.0	0.0	1.5335499561874086	0.0
4	6.555499955204084E-4	0.0	0.0	2.156103935266623	0.0
5	6.555499955204084E-4	0.0	0.0	4.126468705135797	0.0
6	6.555499955204084E-4	0.0	0.0	5.248551780801563	0.0
7	6.555499955204084E-4	0.0	0.0	6.365827489833512	0.0
8	6.555499955204084E-4	0.0	0.0	7.891073812744329	0.0
9	8.740666606938778E-4	0.0	0.0	8.463805992163993	0.0
10	8.740666606938778E-4	0.0	0.0	10.437885545341116	0.0
11	8.740666606938778E-4	0.0	0.0	12.135978550404147	0.0
12	8.740666606938778E-4	0.0	0.0	14.176050136463658	0.0
13	0.0010925833258673473	0.0	0.0	14.787022732288678	0.0
14	0.0010925833258673473	0.0	0.0	15.04093909722025	0.0
15	0.0010925833258673473	0.0	0.0	15.508127727361128	0.0
16	0.0010925833258673473	0.0	0.0	16.315328288511925	0.0
17	0.0010925833258673473	0.0	0.0	17.270246115319985	0.0
18	0.0010925833258673473	0.0	0.0	18.289407841689048	0.0
19	0.0010925833258673473	0.0	0.0	19.014883170064966	0.0
20	0.0013110999910408167	0.0	0.0	19.64180748244765	0.0
21	0.0013110999910408167	0.0	0.0	20.402463993916495	0.0
22	0.0013110999910408167	0.0	0.0	21.203764605107608	0.0
23	0.0013110999910408167	0.0	0.0	22.017302149548435	0.0
24	0.0013110999910408167	0.0	0.0	22.637452445310743	0.0
25	0.0013110999910408167	0.0	0.0	23.17325530831609	0.0
26	0.0013110999910408167	0.0	0.0	23.67781028820163	0.0
27	0.0015296166562142862	0.0	0.0	24.243331417670568	0.0
28	0.0015296166562142862	0.0	0.0	24.795304513898753	0.0
29	0.0017481333213877556	0.0	0.0	25.33809991018965	0.0
30	0.0017481333213877556	0.0	0.0	25.97551302250066	0.0
31	0.0017481333213877556	0.0	0.0	26.5484637185855	0.0
32	0.0017481333213877556	0.0	0.0	27.087981364898795	0.0
33	0.0017481333213877556	0.0	0.0	27.647165511077702	0.0
34	0.0017481333213877556	0.0	0.0	28.14472795767769	0.0
35	0.0017481333213877556	0.0	0.0	28.66676427077711	0.0
36	0.0017481333213877556	0.0	0.0	29.156241600765682	0.0
37	0.0017481333213877556	0.0	0.0	29.663855813963654	0.0
38	0.0017481333213877556	0.0	0.0	30.152677593956703	0.0
39	0.0017481333213877556	0.0	0.0	30.632758707342816	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCT	20	7.02883E-4	45.000004	34
CAACGTA	20	7.02883E-4	45.000004	26
CGTGAGA	20	7.02883E-4	45.000004	19
CTATACG	20	7.02883E-4	45.000004	11
GACGTAG	35	1.2097917E-7	45.000004	1
ATTACGG	40	6.7993824E-9	45.000004	2
TTCACGA	20	7.02883E-4	45.000004	45
GTAGCGA	20	7.02883E-4	45.000004	28
TACGATC	20	7.02883E-4	45.000004	14
AATAGCG	20	7.02883E-4	45.000004	1
TATACGA	20	7.02883E-4	45.000004	12
GGTTACG	20	7.02883E-4	45.000004	1
AACACGT	20	7.02883E-4	45.000004	41
ACGCGCT	20	7.02883E-4	45.000004	25
ACGCATT	25	3.8871134E-5	45.0	35
TTTCGTC	45	3.8380676E-10	45.0	16
CGATTCG	25	3.8871134E-5	45.0	10
TACGAAT	75	0.0	45.0	12
CGAATAT	75	0.0	45.0	14
GCGATAT	25	3.8871134E-5	45.0	9
>>END_MODULE