##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935985.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	605478
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0615133828149	33.0	31.0	34.0	30.0	34.0
2	32.16343120641873	33.0	31.0	34.0	30.0	34.0
3	32.09960725245178	33.0	31.0	34.0	30.0	34.0
4	35.7381837160062	37.0	35.0	37.0	33.0	37.0
5	31.951141081922053	37.0	35.0	37.0	0.0	37.0
6	33.80906820726764	37.0	35.0	37.0	17.0	37.0
7	35.49496100601508	37.0	35.0	37.0	32.0	37.0
8	35.76050822655819	37.0	35.0	37.0	35.0	37.0
9	37.70843036410902	39.0	37.0	39.0	35.0	39.0
10	37.32262443887309	39.0	37.0	39.0	34.0	39.0
11	37.31921556191968	39.0	37.0	39.0	34.0	39.0
12	37.29219558761838	39.0	37.0	39.0	34.0	39.0
13	37.24067431021441	39.0	37.0	39.0	34.0	39.0
14	38.49078248920687	40.0	38.0	41.0	34.0	41.0
15	38.63920076369414	40.0	38.0	41.0	35.0	41.0
16	38.56670432286557	40.0	38.0	41.0	34.0	41.0
17	38.578063942868276	40.0	38.0	41.0	34.0	41.0
18	38.4520048622741	40.0	38.0	41.0	34.0	41.0
19	38.29163239622249	40.0	37.0	41.0	34.0	41.0
20	38.32583182213062	40.0	37.0	41.0	34.0	41.0
21	38.24594948123631	40.0	37.0	41.0	34.0	41.0
22	38.293680364934815	40.0	37.0	41.0	34.0	41.0
23	38.312990727986815	40.0	37.0	41.0	34.0	41.0
24	38.305020165885466	40.0	37.0	41.0	34.0	41.0
25	38.029266133534165	40.0	36.0	41.0	34.0	41.0
26	38.083697508414836	40.0	36.0	41.0	34.0	41.0
27	38.042416074572486	40.0	36.0	41.0	34.0	41.0
28	37.91016023703586	40.0	36.0	41.0	34.0	41.0
29	37.81415840047037	40.0	36.0	41.0	33.0	41.0
30	37.65611136985984	40.0	36.0	41.0	33.0	41.0
31	37.61073730176819	40.0	36.0	41.0	33.0	41.0
32	37.484344600464425	40.0	36.0	41.0	33.0	41.0
33	37.31052490759367	40.0	36.0	41.0	33.0	41.0
34	37.158853005394086	40.0	36.0	41.0	32.0	41.0
35	37.112089952070924	40.0	36.0	41.0	32.0	41.0
36	36.997664655032885	40.0	35.0	41.0	31.0	41.0
37	36.93222049356046	40.0	35.0	41.0	31.0	41.0
38	36.793041200506046	40.0	35.0	41.0	31.0	41.0
39	36.724558117718566	40.0	35.0	41.0	31.0	41.0
40	36.67269826484199	40.0	35.0	41.0	30.0	41.0
41	36.5877240791573	40.0	35.0	41.0	30.0	41.0
42	36.563997370672425	40.0	35.0	41.0	30.0	41.0
43	36.47777953947129	39.0	35.0	41.0	30.0	41.0
44	36.42347698842897	39.0	35.0	41.0	30.0	41.0
45	36.40126808901397	39.0	35.0	41.0	30.0	41.0
46	36.27368459299925	39.0	35.0	41.0	30.0	41.0
47	36.23590617660757	39.0	35.0	41.0	30.0	41.0
48	36.20252263500903	39.0	35.0	41.0	30.0	41.0
49	36.13321210679827	39.0	35.0	41.0	29.0	41.0
50	36.02188683981912	39.0	35.0	41.0	29.0	41.0
51	35.08343820915046	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	9.0
9	17.0
10	23.0
11	27.0
12	13.0
13	3.0
14	11.0
15	23.0
16	27.0
17	66.0
18	133.0
19	256.0
20	471.0
21	832.0
22	1369.0
23	2144.0
24	3312.0
25	5102.0
26	6945.0
27	7757.0
28	7730.0
29	7802.0
30	8851.0
31	10595.0
32	13537.0
33	19366.0
34	31588.0
35	46219.0
36	42235.0
37	66232.0
38	115041.0
39	207572.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97543098180281	19.932681286520733	22.845916779800422	15.24597095187604
2	32.84429822388262	23.927706704454994	25.394481715272892	17.833513356389496
3	31.620141441968165	23.295809261443026	28.69683126389398	16.387218032694832
4	27.437000188280997	25.342787021163442	28.878505907729103	18.34170688282646
5	21.555696491036834	37.27815048606225	24.447131027056308	16.719021995844603
6	24.367854818837348	34.406369843330395	27.215687440336396	14.010087897495863
7	78.23719441499112	5.70359286381999	11.804227403803276	4.254985317385603
8	78.87701947882499	5.746864460806173	11.017080719695842	4.359035340672989
9	73.04972269843	7.478058657787731	13.22112446695008	6.251094176832189
10	34.81695453839776	31.0338278186821	20.358625746930525	13.790591895989616
11	27.223780219925413	26.817654811570364	28.936806952523458	17.02175801598076
12	26.14215545403797	23.01008459432052	32.60167999497917	18.24607995666234
13	23.980062033632933	24.5316923158232	32.59210078648605	18.896144864057817
14	18.77904729816773	29.917519711698855	31.22739389375006	20.07603909638335
15	17.77818516940335	26.57470626513267	37.177568796884444	18.469539768579534
16	20.429313699259097	25.517194679245158	34.595311472918915	19.458180148576826
17	20.27802826857458	24.285440594043052	31.571915081968292	23.86461605541407
18	21.406062648023543	24.961765745411064	32.59738586703398	21.03478573953141
19	22.32021642404844	26.67495763677623	30.381615847314023	20.623210091861306
20	23.977914969660336	25.975345099243903	30.857603414162032	19.18913651693373
21	22.92568846432075	27.28934825047318	31.123839346764044	18.661123938442024
22	20.88333515007977	25.975179940476778	30.41332633060161	22.72815857884184
23	18.534612322825932	27.66277222293791	31.14464935142152	22.657966102814637
24	20.044328613095768	25.488622212532906	32.229246975117185	22.23780219925414
25	19.098299195016168	27.88061663677293	30.60903946964217	22.412044698568735
26	18.54980692940123	28.75381103855136	30.61168200991613	22.084700022131276
27	20.250777071999313	27.837510198553872	30.27343685484857	21.63827587459825
28	19.240996369810297	27.61272911649969	32.52702823223965	20.61924628145036
29	19.218699936248715	27.124024324583225	32.52190831045884	21.135367428709216
30	20.190328963232353	26.938220711570033	30.833820551696345	22.037629773501266
31	20.03293265816429	29.380588559782517	28.46313160841517	22.12334717363802
32	20.700008918573428	29.556978123069705	27.927026250334446	21.81598670802242
33	20.22187428775282	27.13079583403526	29.46052540306997	23.186804475141955
34	19.00878314323559	28.246278147182885	29.49091461622057	23.254024093360947
35	18.747832291181513	27.53295743197936	28.878505907729103	24.840704369110025
36	19.167170400906393	28.449093113209727	28.72887206471581	23.654864421168067
37	18.853203584605883	29.64748512745302	28.612104816359967	22.88720647158113
38	18.87979414611266	29.151348191016023	27.67631524184198	24.292542421029335
39	19.930699381315257	27.077614711021702	29.21757685663228	23.774109051030756
40	21.373361212133226	25.266648829519816	29.95220305279465	23.407786905552307
41	18.163500573100922	26.281879771023885	28.49533756800412	27.059282087871072
42	19.402026167755064	26.522185777187612	28.245617512114396	25.83017054294293
43	20.348220744601786	27.278282613075948	28.2912013318403	24.082295310481967
44	19.63192717159005	27.43039383759608	28.527048051291708	24.410630939522164
45	19.912531916931748	27.247067606089736	27.572100059787473	25.268300417191046
46	19.801710384192326	27.644109282253034	28.616729261839406	23.93745107171524
47	18.119898658580492	26.803451157597795	29.92775955526047	25.148890628561237
48	18.978559088852112	25.364587978423657	29.540462246357425	26.116390686366802
49	19.733169495836346	24.969032731164468	31.03861742292866	24.259180350070523
50	18.86311311063325	25.794991725545767	30.112241898136677	25.229653265684306
51	18.149792395429724	25.394151397738646	28.296156094853984	28.15990011197764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	315.0
1	535.5
2	756.0
3	4539.5
4	8323.0
5	5556.0
6	2789.0
7	2727.5
8	2666.0
9	2742.5
10	2819.0
11	2778.0
12	2737.0
13	2712.5
14	2688.0
15	2755.0
16	2822.0
17	2633.0
18	2444.0
19	2466.0
20	2488.0
21	2698.5
22	2909.0
23	3186.5
24	3464.0
25	3787.0
26	4911.5
27	5713.0
28	6937.5
29	8162.0
30	9075.5
31	9989.0
32	11944.5
33	13900.0
34	15709.0
35	17518.0
36	20048.0
37	22578.0
38	24380.0
39	26182.0
40	28599.0
41	31016.0
42	34025.5
43	37035.0
44	41703.0
45	46371.0
46	52111.0
47	57851.0
48	59330.5
49	60810.0
50	58695.0
51	56580.0
52	48793.5
53	41007.0
54	36399.0
55	31791.0
56	28401.0
57	25011.0
58	23414.0
59	21817.0
60	19988.5
61	18160.0
62	16268.0
63	14376.0
64	11112.0
65	7848.0
66	5905.0
67	3962.0
68	3395.5
69	2829.0
70	2111.0
71	1393.0
72	1116.0
73	839.0
74	838.0
75	547.5
76	258.0
77	198.0
78	138.0
79	106.5
80	75.0
81	72.0
82	69.0
83	47.0
84	25.0
85	15.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	605478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.89390491561466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.87287599145648	25.777131106888756
2	10.439261755506767	7.285332141717314
3	3.8796801384163615	4.061315695586978
4	2.1009561492749813	2.932422564187085
5	1.306110966801011	2.2787655942398013
6	0.868375591298488	1.818060918828606
7	0.7161011500150262	1.7491295807010647
8	0.5518472651285589	1.5404884797870344
9	0.4528834548733588	1.422258499098544
>10	5.568583821667634	42.732160148266004
>50	0.20787892235004624	4.453174623152896
>100	0.03065495629086151	1.82947190453778
>500	0.0028739021522682664	0.713504070936578
>1k	0.0019159347681788443	1.4067846720714867
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4503	0.7437099283541269	No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1636	0.27019974301295835	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1231	0.2033104423282101	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	1047	0.17292122917760844	TruSeq Adapter, Index 13 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	928	0.15326733588999106	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG	785	0.1296496321914256	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC	708	0.1169324071229673	No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	670	0.11065637397229956	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC	625	0.103224229451772	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.909526027370111E-4	0.0	0.0	0.18101400876662735	0.0
2	9.909526027370111E-4	0.0	0.0	0.8781491647921147	0.0
3	9.909526027370111E-4	0.0	0.0	1.1686634361611818	0.0
4	9.909526027370111E-4	0.0	0.0	1.532343041365665	0.0
5	9.909526027370111E-4	0.0	0.0	2.6215651105407627	0.0
6	9.909526027370111E-4	0.0	0.0	3.3963248871139826	0.0
7	9.909526027370111E-4	0.0	0.0	4.094450995742207	0.0
8	9.909526027370111E-4	0.0	0.0	5.147338136150282	0.0
9	9.909526027370111E-4	0.0	0.0	5.585174027792918	0.0
10	9.909526027370111E-4	0.0	0.0	6.674396096968016	0.0
11	9.909526027370111E-4	0.0	0.0	8.049342833265618	0.0
12	9.909526027370111E-4	0.0	0.0	9.295630889974532	0.0
13	9.909526027370111E-4	0.0	0.0	9.831075612986764	0.0
14	9.909526027370111E-4	0.0	0.0	10.063123680794348	0.0
15	9.909526027370111E-4	0.0	0.0	10.43027162010841	0.0
16	9.909526027370111E-4	0.0	0.0	11.13516923818867	0.0
17	9.909526027370111E-4	0.0	0.0	12.009354592569837	0.0
18	9.909526027370111E-4	0.0	0.0	12.97949719064937	0.0
19	9.909526027370111E-4	0.0	0.0	13.548303984620416	0.0
20	9.909526027370111E-4	0.0	0.0	14.072683070235417	0.0
21	9.909526027370111E-4	0.0	0.0	14.71647194448023	0.0
22	0.0011561113698598463	0.0	0.0	15.40155051050575	0.0
23	0.0011561113698598463	0.0	0.0	16.09587796749015	0.0
24	0.0011561113698598463	0.0	0.0	16.650315948721506	0.0
25	0.0011561113698598463	0.0	0.0	17.135552406528397	0.0
26	0.0013212701369826815	0.0	0.0	17.61864180036269	0.0
27	0.0013212701369826815	0.0	0.0	18.06969039337515	0.0
28	0.0013212701369826815	0.0	0.0	18.500589616798628	0.0
29	0.0013212701369826815	0.0	0.0	18.984669963235657	0.0
30	0.0013212701369826815	0.0	0.0	19.558101202686142	0.0
31	0.0013212701369826815	0.0	0.0	20.07769068405458	0.0
32	0.0013212701369826815	0.0	0.0	20.56226650679298	0.0
33	0.0013212701369826815	0.0	0.0	21.061541459805312	0.0
34	0.0013212701369826815	0.0	0.0	21.56015577774915	0.0
35	0.0013212701369826815	0.0	0.0	22.069175098021727	0.0
36	0.0013212701369826815	0.0	0.0	22.550943221719038	0.0
37	0.0014864289041055167	0.0	0.0	23.031885551580736	0.0
38	0.0014864289041055167	0.0	0.0	23.497963592401376	0.0
39	0.0014864289041055167	0.0	0.0	24.00863450034518	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTCG	25	3.8886104E-5	45.000004	10
CGATTAC	25	3.8886104E-5	45.000004	10
CGGAGTA	25	3.8886104E-5	45.000004	25
TACACGG	55	1.8189894E-12	45.000004	2
GCATACG	55	1.8189894E-12	45.000004	1
AAGTACG	20	7.0306385E-4	45.0	1
CAGCGCG	35	1.2106466E-7	45.0	1
CGAACTA	20	7.0306385E-4	45.0	40
CGCGGCC	20	7.0306385E-4	45.0	13
TTCGTCA	20	7.0306385E-4	45.0	39
ATTCGAT	35	1.2106466E-7	45.0	14
TCGGCCC	20	7.0306385E-4	45.0	40
CGCTCAA	20	7.0306385E-4	45.0	26
AACGACC	20	7.0306385E-4	45.0	40
ATGCGTC	20	7.0306385E-4	45.0	39
ATGCGCA	20	7.0306385E-4	45.0	20
GCTAACG	35	1.2106466E-7	45.0	1
TCGCGAG	35	1.2106466E-7	45.0	1
CGTTTTT	2415	0.0	42.577644	1
TACGAAT	80	0.0	42.1875	12
>>END_MODULE