FastQCFastQC Report
Sat 14 Jan 2017
SRR2935984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314615
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46101.4652829648936No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG42991.3664319883031641No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC37481.1912972998744498No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC34231.0879964400934476No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC19510.6201230074853392Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC10850.3448659472688842RNA PCR Primer, Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT8630.27430351381847656No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT7940.2523719466649715TruSeq Adapter, Index 10 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT6520.20723741716065666TruSeq Adapter, Index 10 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTC6420.20405892916739507No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTA5820.18498800120782544No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGATTACCT5750.1827630596125423No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC5430.17259189803410518Illumina PCR Primer Index 2 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.15987794606105873No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG4810.1528852724758832No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC3850.1223717877405718No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC3420.1087042893695469No Hit
GAATATGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC3240.102983010981676No Hit
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC3240.102983010981676TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCGA600.045.00000441
ACGCATG302.1604574E-645.0000041
CACGAGG302.1604574E-645.0000042
TACGAAT551.8189894E-1245.00000412
AGACCGG302.1604574E-645.00000411
GTCGTCA302.1604574E-645.0000049
CGAATAT551.8189894E-1245.00000414
GGGCCGA600.045.0000047
TAGATCC302.1604574E-645.00000436
GGGTTCG302.1604574E-645.0000047
CGGAATA253.8843267E-545.06
CCCCTCG700.045.040
GAATGCG207.0254673E-445.01
ACGCATT207.0254673E-445.025
CTATCAT207.0254673E-445.013
GTGGGCG207.0254673E-445.05
CGGCAGG351.2081728E-745.02
TTCACTC207.0254673E-445.020
CCGGATA207.0254673E-445.019
GGCCGAT502.1827873E-1145.08
Produced by FastQC (version 0.11.5)