##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935984.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314615
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97857063394943	33.0	31.0	34.0	30.0	34.0
2	32.11637080240929	33.0	31.0	34.0	30.0	34.0
3	32.00835942342228	33.0	31.0	34.0	30.0	34.0
4	35.70128569839328	37.0	35.0	37.0	33.0	37.0
5	31.963828806636684	37.0	35.0	37.0	0.0	37.0
6	33.79295647060693	37.0	35.0	37.0	17.0	37.0
7	35.44518538531221	37.0	35.0	37.0	32.0	37.0
8	35.63927339764474	37.0	35.0	37.0	33.0	37.0
9	37.60372836641609	39.0	37.0	39.0	35.0	39.0
10	37.36301511371041	39.0	37.0	39.0	34.0	39.0
11	37.24992451091016	39.0	37.0	39.0	34.0	39.0
12	37.03010981676017	39.0	35.0	39.0	34.0	39.0
13	36.804484846558495	39.0	35.0	39.0	33.0	39.0
14	37.95549798960634	40.0	36.0	41.0	33.0	41.0
15	38.089973459625256	40.0	36.0	41.0	33.0	41.0
16	38.18573494588624	40.0	36.0	41.0	34.0	41.0
17	38.11299206967246	40.0	36.0	41.0	34.0	41.0
18	37.97767430033533	39.0	36.0	41.0	34.0	41.0
19	37.775439823276066	39.0	36.0	41.0	34.0	41.0
20	37.58747040033056	39.0	35.0	41.0	33.0	41.0
21	37.63486801328608	39.0	35.0	41.0	33.0	41.0
22	37.6654228183653	39.0	35.0	41.0	34.0	41.0
23	37.631880234572414	39.0	35.0	41.0	34.0	41.0
24	37.59334424614211	39.0	35.0	41.0	33.0	41.0
25	37.433116666401794	39.0	35.0	41.0	33.0	41.0
26	37.40763472815981	39.0	35.0	41.0	33.0	41.0
27	37.35944249320598	39.0	35.0	41.0	33.0	41.0
28	37.29038348457639	39.0	35.0	41.0	33.0	41.0
29	37.2725458099582	39.0	35.0	41.0	33.0	41.0
30	37.07841012030577	39.0	35.0	41.0	33.0	41.0
31	36.93365224162866	39.0	35.0	41.0	32.0	41.0
32	36.667892503536066	39.0	35.0	41.0	32.0	41.0
33	36.40797800486309	39.0	35.0	41.0	31.0	41.0
34	36.13260016210289	39.0	35.0	41.0	30.0	41.0
35	36.047038443812276	39.0	35.0	41.0	30.0	41.0
36	35.89624143794797	39.0	35.0	41.0	29.0	41.0
37	35.755841266309616	39.0	35.0	41.0	28.0	41.0
38	35.646733308964926	39.0	35.0	41.0	27.0	41.0
39	35.577652050919376	39.0	35.0	41.0	27.0	41.0
40	35.50080574670629	39.0	35.0	41.0	26.0	41.0
41	35.35513564197511	38.0	35.0	41.0	25.0	41.0
42	35.362735406767	38.0	35.0	41.0	25.0	41.0
43	35.288606709788155	38.0	35.0	41.0	25.0	41.0
44	35.22614942072056	38.0	35.0	41.0	24.0	41.0
45	35.23745530251259	38.0	35.0	41.0	24.0	41.0
46	35.133795273588355	38.0	35.0	40.0	24.0	41.0
47	34.97111390111724	38.0	35.0	40.0	23.0	41.0
48	34.976345692354144	38.0	35.0	40.0	23.0	41.0
49	34.99535940752984	38.0	35.0	40.0	24.0	41.0
50	34.879249241135994	38.0	35.0	40.0	24.0	41.0
51	33.89205219077285	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	10.0
11	11.0
12	8.0
13	7.0
14	8.0
15	16.0
16	24.0
17	49.0
18	100.0
19	220.0
20	412.0
21	857.0
22	1301.0
23	1878.0
24	2602.0
25	3697.0
26	4867.0
27	5324.0
28	5303.0
29	5050.0
30	5428.0
31	6656.0
32	8615.0
33	12618.0
34	21476.0
35	32101.0
36	22912.0
37	31852.0
38	52611.0
39	88541.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35271681261224	17.407943041495162	22.167728811404412	18.071611334488182
2	34.19131319231442	27.55590165758149	22.060931614830825	16.19185353527327
3	28.052699330928277	27.341671566835657	30.11045245776584	14.495176644470225
4	25.350666687856588	21.385185067463407	36.07965290911114	17.18449533556887
5	24.16731560796529	33.38048090523338	25.934554932218745	16.517648554582586
6	22.327288908666148	34.53903977877724	29.569473801312718	13.5641975112439
7	69.25607488517713	5.260397628847957	20.74758037601513	4.735947109959792
8	68.64930152726349	9.43502375919775	16.208699521637556	5.706975191901212
9	64.4591008057467	6.595680434817157	17.1860845795655	11.759134179870635
10	41.923620933521924	21.22053938941246	23.66384310983265	13.191996567232966
11	31.948254215469703	20.512054415714445	30.02463328194778	17.515058086868077
12	27.316243662889562	18.669167077221367	34.502805015654054	19.511784244235017
13	22.85682500834353	23.93751092605248	36.83263671471481	16.37302735088918
14	16.700411614195126	29.74460848974143	34.14363587241549	19.41134402364795
15	14.875641657263639	22.220809560891883	43.171177470877105	19.732371310967373
16	16.783370150819255	20.807335950288447	38.95523099661491	23.454062902277386
17	16.696597428603212	22.340956407037172	35.4595299016258	25.502916262733816
18	18.435548209716636	22.415333026079495	36.658455572684076	22.490663191519793
19	20.52635761168412	23.76428333041972	32.52928182063792	23.18007723725824
20	22.895602561861324	22.96711854170971	34.683343133671315	19.453935762757656
21	20.0546699934841	27.39793080431639	33.806398296330435	18.74100090586908
22	18.529949303116506	22.276115251974634	34.10644756289433	25.087487882014525
23	18.77310363460102	25.911987667466583	33.12397692417717	22.190931773755224
24	19.883667339446625	22.38100535575227	34.15889261478315	23.576434690017958
25	16.676255105446337	27.608982407068954	33.530823387314655	22.18393910017005
26	16.794812707594996	25.122133401141078	35.49640036234763	22.586653528916294
27	21.126773993611238	25.863992498768333	31.719720928754192	21.289512578866233
28	15.583173084563672	25.376412440602003	37.741366431988304	21.29904804284602
29	18.687920156381608	20.729780843252865	39.47936366670375	21.102935333661776
30	20.214230090745833	25.702843157509975	35.004688269790066	19.078238481954134
31	21.467507906488883	26.46059469510354	30.396834225958713	21.675063172448866
32	23.41814598795353	25.49369864755336	31.668547272062682	19.419608092430433
33	22.000222494159527	24.209907347074996	31.0029718862737	22.786898272491776
34	18.87672234318135	25.82457924765189	33.14590849133067	22.152789917836085
35	20.129364461325746	22.72015002463328	31.841457018896115	25.309028495144858
36	21.101663938464473	26.110961015844765	31.475930899671027	21.31144414601974
37	19.891931408229105	25.10369817078016	34.42715700141443	20.577213419576307
38	18.73082974429064	26.452330626321057	30.31673632852852	24.50010330085978
39	19.854743098707946	25.734628037442587	32.514978624668245	21.895650239181222
40	20.699903056116206	23.11841457018896	33.090284951448595	23.09139742224624
41	18.160926847098835	26.13543537339288	27.960841027923017	27.74279675158527
42	19.180267946537832	22.895602561861324	31.64788710010648	26.276242391494364
43	22.598731783290688	23.055162659123056	28.98526770815123	25.360837849435026
44	20.254914737059583	23.804014430335492	31.616102220173865	24.324968612431068
45	19.22540247604215	24.387266977098996	30.13619821051126	26.2511323363476
46	22.839661173179916	26.23619344277927	29.66006070912067	21.26408467492014
47	17.86246682453157	23.85200959903374	35.169016099041684	23.116507477393004
48	20.37410803680689	23.236336474738966	30.674951925369104	25.714603563085042
49	19.02706482526262	20.430367274287622	36.290386663064375	24.252181237385376
50	19.425965068416954	22.187753285761964	32.74796179457432	25.63831985124676
51	18.542345406290227	23.49856173418305	29.34888673458036	28.610206124946362
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	396.0
1	500.5
2	605.0
3	4357.0
4	8109.0
5	5226.5
6	2344.0
7	2322.5
8	2301.0
9	2263.0
10	2225.0
11	2256.5
12	2288.0
13	2171.0
14	2054.0
15	1947.0
16	1840.0
17	1760.5
18	1681.0
19	1698.5
20	1716.0
21	1656.5
22	1597.0
23	1609.5
24	1622.0
25	1749.5
26	2098.0
27	2319.0
28	2687.0
29	3055.0
30	3209.0
31	3363.0
32	4150.5
33	4938.0
34	6122.0
35	7306.0
36	7452.0
37	7598.0
38	8571.5
39	9545.0
40	11596.5
41	13648.0
42	17772.5
43	21897.0
44	32108.0
45	42319.0
46	37435.5
47	32552.0
48	31608.0
49	30664.0
50	25613.5
51	20563.0
52	18210.0
53	15857.0
54	14793.0
55	13729.0
56	12976.0
57	12223.0
58	11014.0
59	9805.0
60	9355.0
61	8905.0
62	8267.0
63	7629.0
64	6786.5
65	5944.0
66	4885.0
67	3826.0
68	3151.0
69	2476.0
70	1991.5
71	1507.0
72	1166.5
73	826.0
74	758.0
75	472.5
76	255.0
77	258.0
78	261.0
79	215.0
80	169.0
81	100.0
82	31.0
83	40.5
84	50.0
85	28.5
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	314615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.60932846424071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.36662524227924	23.924363808383372
2	10.883742748685822	7.09823084424388
3	4.187176702356651	4.096230612748929
4	2.1962837735771763	2.8647735589304086
5	1.346879665324967	2.1960420704194226
6	0.9072414194319904	1.7750720065573051
7	0.6674416760243217	1.5235377390940308
8	0.5075753899886504	1.324135409000398
9	0.3956690036979087	1.161225045423358
>10	5.289575012709462	39.813337026796106
>50	0.19883366594777968	4.0479966219338
>100	0.03896740186916285	2.2673863886057077
>500	0.007993313203930843	1.6792799894918546
>1k	0.005994984902948131	6.228388878371419
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4610	1.4652829648936	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	4299	1.3664319883031641	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	3748	1.1912972998744498	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	3423	1.0879964400934476	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1951	0.6201230074853392	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1085	0.3448659472688842	RNA PCR Primer, Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	863	0.27430351381847656	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	794	0.2523719466649715	TruSeq Adapter, Index 10 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	652	0.20723741716065666	TruSeq Adapter, Index 10 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTC	642	0.20405892916739507	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTA	582	0.18498800120782544	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGATTACCT	575	0.1827630596125423	No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	543	0.17259189803410518	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	503	0.15987794606105873	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	481	0.1528852724758832	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	385	0.1223717877405718	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	342	0.1087042893695469	No Hit
GAATATGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	324	0.102983010981676	No Hit
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	324	0.102983010981676	TruSeq Adapter, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1784879932616057E-4	0.0	0.0	0.26158956184543014	0.0
2	3.1784879932616057E-4	0.0	0.0	1.5539627799055988	0.0
3	3.1784879932616057E-4	0.0	0.0	1.9417383150835148	0.0
4	3.1784879932616057E-4	0.0	0.0	2.604135212879233	0.0
5	3.1784879932616057E-4	0.0	0.0	5.106876658773421	0.0
6	3.1784879932616057E-4	0.0	0.0	5.980007310522384	0.0
7	3.1784879932616057E-4	0.0	0.0	7.122673744099932	0.0
8	3.1784879932616057E-4	0.0	0.0	8.520254914737059	0.0
9	3.1784879932616057E-4	0.0	0.0	8.980182127362013	0.0
10	3.1784879932616057E-4	0.0	0.0	11.533143683549735	0.0
11	3.1784879932616057E-4	0.0	0.0	13.182461103253182	0.0
12	3.1784879932616057E-4	0.0	0.0	15.61908999888753	0.0
13	3.1784879932616057E-4	0.0	0.0	16.188675047280007	0.0
14	3.1784879932616057E-4	0.0	0.0	16.425790251577325	0.0
15	3.1784879932616057E-4	0.0	0.0	16.965815361632473	0.0
16	3.1784879932616057E-4	0.0	0.0	17.768383579931026	0.0
17	3.1784879932616057E-4	0.0	0.0	18.683788121990368	0.0
18	3.1784879932616057E-4	0.0	0.0	19.636380973570873	0.0
19	3.1784879932616057E-4	0.0	0.0	20.435135006277513	0.0
20	3.1784879932616057E-4	0.0	0.0	21.007898542663256	0.0
21	3.1784879932616057E-4	0.0	0.0	21.666799103666385	0.0
22	6.356975986523211E-4	0.0	0.0	22.40738680609634	0.0
23	6.356975986523211E-4	0.0	0.0	23.042766555949335	0.0
24	6.356975986523211E-4	0.0	0.0	23.57865963161324	0.0
25	6.356975986523211E-4	0.0	0.0	24.011887545094798	0.0
26	6.356975986523211E-4	0.0	0.0	24.468318420927165	0.0
27	6.356975986523211E-4	0.0	0.0	24.928881331150773	0.0
28	6.356975986523211E-4	0.0	0.0	25.315385471131382	0.0
29	6.356975986523211E-4	0.0	0.0	25.768002161371836	0.0
30	6.356975986523211E-4	0.0	0.0	26.286095704273478	0.0
31	6.356975986523211E-4	0.0	0.0	26.767000937653957	0.0
32	6.356975986523211E-4	0.0	0.0	27.22565675508161	0.0
33	6.356975986523211E-4	0.0	0.0	27.64998490218203	0.0
34	6.356975986523211E-4	0.0	0.0	28.07749153727572	0.0
35	6.356975986523211E-4	0.0	0.0	28.591135196986794	0.0
36	6.356975986523211E-4	0.0	0.0	29.03167363285285	0.0
37	6.356975986523211E-4	0.0	0.0	29.44138073518427	0.0
38	6.356975986523211E-4	0.0	0.0	29.893997425424725	0.0
39	9.535463979784817E-4	0.0	0.0	30.327543187705608	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGA	60	0.0	45.000004	41
ACGCATG	30	2.1604574E-6	45.000004	1
CACGAGG	30	2.1604574E-6	45.000004	2
TACGAAT	55	1.8189894E-12	45.000004	12
AGACCGG	30	2.1604574E-6	45.000004	11
GTCGTCA	30	2.1604574E-6	45.000004	9
CGAATAT	55	1.8189894E-12	45.000004	14
GGGCCGA	60	0.0	45.000004	7
TAGATCC	30	2.1604574E-6	45.000004	36
GGGTTCG	30	2.1604574E-6	45.000004	7
CGGAATA	25	3.8843267E-5	45.0	6
CCCCTCG	70	0.0	45.0	40
GAATGCG	20	7.0254673E-4	45.0	1
ACGCATT	20	7.0254673E-4	45.0	25
CTATCAT	20	7.0254673E-4	45.0	13
GTGGGCG	20	7.0254673E-4	45.0	5
CGGCAGG	35	1.2081728E-7	45.0	2
TTCACTC	20	7.0254673E-4	45.0	20
CCGGATA	20	7.0254673E-4	45.0	19
GGCCGAT	50	2.1827873E-11	45.0	8
>>END_MODULE