##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935983.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1271727
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07033349138612	33.0	31.0	34.0	30.0	34.0
2	32.22035861470268	33.0	31.0	34.0	30.0	34.0
3	32.095934897977315	33.0	31.0	34.0	30.0	34.0
4	35.779452665548504	37.0	35.0	37.0	35.0	37.0
5	32.10217365834019	37.0	35.0	37.0	0.0	37.0
6	33.86090961346264	37.0	35.0	37.0	17.0	37.0
7	35.485317210376124	37.0	35.0	37.0	32.0	37.0
8	35.70071485468186	37.0	35.0	37.0	35.0	37.0
9	37.66044441928181	39.0	37.0	39.0	35.0	39.0
10	37.32651425974285	39.0	37.0	39.0	34.0	39.0
11	37.2784544167105	39.0	37.0	39.0	34.0	39.0
12	37.20759329635999	39.0	37.0	39.0	34.0	39.0
13	37.13289880611169	39.0	37.0	39.0	33.0	39.0
14	38.302694682113376	40.0	38.0	41.0	33.0	41.0
15	38.41695190870367	40.0	38.0	41.0	34.0	41.0
16	38.43782824458394	40.0	38.0	41.0	34.0	41.0
17	38.39334228179476	40.0	38.0	41.0	34.0	41.0
18	38.22996602258189	40.0	37.0	41.0	34.0	41.0
19	38.02439438653107	40.0	37.0	41.0	34.0	41.0
20	37.87735024891349	40.0	35.0	41.0	33.0	41.0
21	37.86169594574936	40.0	35.0	41.0	33.0	41.0
22	37.910446188529455	40.0	35.0	41.0	34.0	41.0
23	37.88063475887514	40.0	35.0	41.0	34.0	41.0
24	37.84843916972746	40.0	35.0	41.0	33.0	41.0
25	37.64957494808241	40.0	35.0	41.0	33.0	41.0
26	37.63056615138312	40.0	35.0	41.0	33.0	41.0
27	37.59022573240955	40.0	35.0	41.0	33.0	41.0
28	37.493495852490355	40.0	35.0	41.0	33.0	41.0
29	37.45489951852874	40.0	35.0	41.0	33.0	41.0
30	37.25495802165087	40.0	35.0	41.0	33.0	41.0
31	37.12432463885724	40.0	35.0	41.0	32.0	41.0
32	36.86427197032067	40.0	35.0	41.0	31.0	41.0
33	36.62982621270131	40.0	35.0	41.0	31.0	41.0
34	36.3341173066232	39.0	35.0	41.0	30.0	41.0
35	36.178980237110636	39.0	35.0	41.0	29.0	41.0
36	36.0290840722891	39.0	35.0	41.0	28.0	41.0
37	35.952341186433884	39.0	35.0	41.0	28.0	41.0
38	35.79156139643178	39.0	35.0	41.0	27.0	41.0
39	35.6349680395242	39.0	35.0	41.0	26.0	41.0
40	35.56601220230443	39.0	35.0	41.0	25.0	41.0
41	35.48372960548923	39.0	35.0	41.0	25.0	41.0
42	35.423355798846764	39.0	35.0	41.0	25.0	41.0
43	35.358695694909365	39.0	35.0	41.0	24.0	41.0
44	35.334753449443156	39.0	35.0	41.0	24.0	41.0
45	35.33109149998388	39.0	35.0	41.0	24.0	41.0
46	35.201341168348236	38.0	35.0	41.0	24.0	41.0
47	35.08687084570824	38.0	35.0	41.0	23.0	41.0
48	35.05793932188276	38.0	35.0	40.0	23.0	41.0
49	35.049406043907226	38.0	35.0	40.0	24.0	41.0
50	34.92307704405112	38.0	35.0	40.0	24.0	41.0
51	33.97963871176754	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	29.0
9	37.0
10	53.0
11	47.0
12	34.0
13	31.0
14	46.0
15	60.0
16	115.0
17	203.0
18	437.0
19	954.0
20	1813.0
21	3347.0
22	5553.0
23	8234.0
24	11450.0
25	15627.0
26	19508.0
27	20779.0
28	20364.0
29	19665.0
30	21117.0
31	25056.0
32	32635.0
33	46345.0
34	77163.0
35	94878.0
36	97346.0
37	141230.0
38	224995.0
39	382266.0
40	305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6632233175831	17.648520476485913	20.607252971746295	18.081003234184696
2	35.88356620564005	25.369517199839276	22.55515531242161	16.19176128209907
3	29.059774621439978	26.026026026026027	29.95768745965132	14.95651189288267
4	25.95045949327175	23.184378408259004	33.74592188417797	17.119240214291274
5	23.933988977194005	34.23439150069158	25.766850904321448	16.06476861779297
6	23.5519101190743	34.19137912460772	28.728964628414744	13.527746127903237
7	73.26588174977806	5.3198524526097195	17.25087223908905	4.163393558523174
8	74.50262517033923	7.762436434863772	13.858634754157142	3.876303640639854
9	69.18127868638474	6.400744813941986	15.264675516050222	9.153300983623057
10	39.240733270584016	23.642181065590336	22.662568302788255	14.454517361037391
11	29.750567535327942	23.042917229877165	29.14422670903425	18.06228852576064
12	24.996245263330888	20.469330288654717	33.75189800955709	20.78252643845731
13	24.745169364179574	21.303235678726644	35.8708276225951	18.080767334498677
14	19.759665399885353	25.79924779453452	34.70705583824201	19.734030967338114
15	18.643230819193114	23.31687539857218	37.95358595044377	20.086307831790943
16	22.21404436644028	23.327962683815002	32.95369210530247	21.50430084444224
17	22.751895650560222	24.21533866938423	32.163506790372466	20.869258889683085
18	22.54414666040746	22.903185982526125	32.776688707560666	21.77597864950575
19	22.841773430932896	24.825375257425534	30.382935960312235	21.949915351329334
20	24.679746517924052	25.238435607642206	31.995703480385334	18.08611439404841
21	22.648728854541893	26.5746500624741	32.54975320961181	18.226867873372193
22	22.061024103443586	22.041208529818114	34.3401531932561	21.5576141734822
23	22.3238163536671	24.48355661238615	33.046007515763996	20.146619518182753
24	21.764262298433547	24.654505251520177	32.250081975140894	21.33115047490538
25	21.613601032297026	27.681570022496967	30.376723935247107	20.3281050099589
26	20.622979617480798	24.506596148387196	32.190320721349785	22.680103512782225
27	22.326804416356655	24.050444788857988	31.67708163780434	21.94566915698102
28	19.13177906893539	26.039315041671678	32.65520036926164	22.173705520131286
29	21.20950487014902	23.62566808756911	33.468110687278006	21.696716355003865
30	21.27799441232277	25.169552899325094	32.19692591255828	21.355526775793862
31	22.053239413805006	24.994908498443454	30.307369427557955	22.644482660193578
32	24.083313478443095	25.034461012465727	30.536192122994947	20.34603338609623
33	23.932337679391882	24.47357019234474	28.897003838087894	22.697088290175486
34	21.249607816771995	24.828520586572434	30.951061037471096	22.97081055918448
35	22.06023777115686	25.883778515357463	29.396088940472286	22.659894773013388
36	22.01109200323654	27.887667714847602	28.131902523104408	21.969337758811445
37	21.126310914213505	26.380740520567702	29.68789685207596	22.805051713142838
38	22.269873958797763	26.27718055840601	28.804137995025663	22.64880748777057
39	22.0747062852326	25.07503575846074	29.2553354611485	23.59492249515816
40	23.16283290360274	25.156499783365454	29.82707766682629	21.853589646205513
41	20.0727042832306	25.401285024222965	29.220500940846584	25.305509751699855
42	20.8892317297659	25.9039872551263	30.366265715833666	22.840515299274138
43	22.100576617465855	24.7090767122189	29.509399422989368	23.680947247325882
44	22.245576291137954	24.403114819454174	29.910507522447823	23.44080136696005
45	21.26234639981694	24.59977652436411	28.557858722823376	25.58001835299557
46	22.382869908400153	25.919399367946106	29.027928163827614	22.669802559826127
47	20.062796496417864	25.60879811468971	32.21398932318021	22.114416065712216
48	21.150765848330654	25.985844446174376	29.835019622922214	23.028370082572753
49	21.337991565799893	24.511392775336216	31.581542264967243	22.569073393896648
50	20.794635955672877	24.764906304576375	30.84687200948002	23.59358573027073
51	20.610791467036556	24.49244216722614	29.54258264548917	25.354183720248137
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	673.0
1	1311.5
2	1950.0
3	18003.0
4	34056.0
5	21711.0
6	9366.0
7	9039.5
8	8713.0
9	8500.5
10	8288.0
11	8132.5
12	7977.0
13	7783.5
14	7590.0
15	7114.5
16	6639.0
17	6305.0
18	5971.0
19	5910.0
20	5849.0
21	6284.5
22	6720.0
23	6909.0
24	7098.0
25	8162.5
26	10513.0
27	11799.0
28	13508.0
29	15217.0
30	18395.0
31	21573.0
32	23425.5
33	25278.0
34	29813.5
35	34349.0
36	36723.0
37	39097.0
38	40930.0
39	42763.0
40	46315.0
41	49867.0
42	55230.5
43	60594.0
44	67152.5
45	73711.0
46	95640.0
47	117569.0
48	106529.0
49	95489.0
50	95879.5
51	96270.0
52	88355.5
53	80441.0
54	75710.5
55	70980.0
56	67374.0
57	63768.0
58	61106.5
59	58445.0
60	54800.0
61	51155.0
62	46865.0
63	42575.0
64	37674.0
65	32773.0
66	27493.0
67	22213.0
68	19214.0
69	16215.0
70	13788.0
71	11361.0
72	9374.0
73	7387.0
74	6173.0
75	3958.5
76	2958.0
77	2244.5
78	1531.0
79	1143.0
80	755.0
81	561.5
82	368.0
83	220.0
84	72.0
85	51.5
86	31.0
87	28.0
88	25.0
89	20.0
90	15.0
91	9.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1271727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.46397182046972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50541293796364	21.657614154561514
2	8.921982413525809	5.257540768297017
3	3.7200552168498353	3.2882280623956475
4	2.179283896398484	2.5684143724915356
5	1.400552742685627	2.0632923271785444
6	0.9909276404753913	1.7517998445054885
7	0.7349361155266473	1.515789589840583
8	0.5851350624607123	1.3792322393208973
9	0.4913170577053032	1.3028536748830635
>10	7.267613978328637	48.780056987001714
>50	0.16357993148681277	2.9289346866831467
>100	0.03217350197109167	1.7158060703034432
>500	0.0029740211862142292	0.6867525938655741
>1k	0.0029740211862142292	1.4520503747803832
>5k	5.407311247662235E-4	1.1260798824827463
>10k+	5.407311247662235E-4	2.525554371408672
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20163	1.5854817897237379	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	11541	0.9075060921093914	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	7610	0.5983988701977704	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	6526	0.5131604503167739	No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	3367	0.2647580809403276	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2569	0.20200876445966784	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2309	0.1815641250048163	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	1553	0.12211740412840176	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	1502	0.11810710946610398	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC	1295	0.10183003113089523	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1288	0.1012795985301877	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.358996860175179E-4	0.0	0.0	0.060390319620484585	0.0
2	2.358996860175179E-4	0.0	0.0	0.3574666575452121	0.0
3	2.358996860175179E-4	0.0	0.0	0.519215208924557	0.0
4	2.358996860175179E-4	0.0	0.0	0.9367576531755636	0.0
5	2.358996860175179E-4	0.0	0.0	2.2676250484577274	0.0
6	2.358996860175179E-4	0.0	0.0	3.043577748998016	0.0
7	2.358996860175179E-4	0.0	0.0	3.6650161551968305	0.0
8	3.145329146900239E-4	0.0	0.0	4.4273653071767765	0.0
9	3.145329146900239E-4	0.0	0.0	4.83507859784372	0.0
10	3.145329146900239E-4	0.0	0.0	6.073080150063654	0.0
11	3.145329146900239E-4	0.0	0.0	6.730925741137838	0.0
12	3.145329146900239E-4	0.0	0.0	7.828960146320712	0.0
13	3.145329146900239E-4	0.0	0.0	8.117072296176774	0.0
14	3.145329146900239E-4	0.0	0.0	8.30083815158442	0.0
15	3.145329146900239E-4	0.0	0.0	8.547431956701399	0.0
16	3.145329146900239E-4	0.0	0.0	8.82044652665234	0.0
17	3.145329146900239E-4	0.0	0.0	9.116579265832998	0.0
18	3.145329146900239E-4	0.0	0.0	9.456982512756275	0.0
19	3.145329146900239E-4	0.0	0.0	9.784018110805228	0.0
20	3.145329146900239E-4	0.0	0.0	10.028095652604687	0.0
21	3.145329146900239E-4	0.0	0.0	10.296313595606604	0.0
22	3.145329146900239E-4	0.0	0.0	10.637031375444572	0.0
23	3.145329146900239E-4	0.0	0.0	10.916022070774623	0.0
24	3.145329146900239E-4	0.0	0.0	11.171501430731595	0.0
25	3.145329146900239E-4	0.0	0.0	11.403154922400798	0.0
26	3.145329146900239E-4	0.0	0.0	11.621676664881692	0.0
27	3.931661433625298E-4	0.0	0.0	11.886198846136002	0.0
28	3.931661433625298E-4	0.0	0.0	12.119189102692637	0.0
29	4.717993720350358E-4	0.0	0.0	12.385441214977742	0.0
30	4.717993720350358E-4	0.0	0.0	12.778056925739564	0.0
31	4.717993720350358E-4	0.0	0.0	13.065382743308902	0.0
32	4.717993720350358E-4	0.0	0.0	13.354045325765671	0.0
33	4.717993720350358E-4	0.0	0.0	13.623128234282987	0.0
34	4.717993720350358E-4	0.0	0.0	13.91218398288312	0.0
35	4.717993720350358E-4	0.0	0.0	14.228053662460574	0.0
36	5.504326007075418E-4	0.0	0.0	14.499810100752756	0.0
37	5.504326007075418E-4	0.0	0.0	14.771723805502281	0.0
38	5.504326007075418E-4	0.0	0.0	15.096400406691059	0.0
39	5.504326007075418E-4	0.0	0.0	15.624343903998264	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAA	90	0.0	45.000004	41
CGTTTTT	8460	0.0	43.164894	1
CGTTATT	1015	0.0	42.78325	1
TAATACG	395	0.0	42.151897	4
ACGTAAG	75	0.0	42.000004	1
CGATGAA	1675	0.0	41.10448	19
CCGATGA	1680	0.0	40.3125	18
TAGCCGT	45	1.9284926E-8	40.000004	44
TACGGGA	390	0.0	39.807693	4
CCGTAAG	85	0.0	39.705883	42
CTCGAAT	205	0.0	39.51219	43
GATGAAT	1865	0.0	38.967827	20
ACGGGTA	145	0.0	38.793106	5
CGTAAGG	175	0.0	38.571426	2
TACGGCT	830	0.0	37.68072	7
TATTGCG	90	0.0	37.500004	1
AAGGGCG	450	0.0	37.5	5
ATATGCG	60	1.5643309E-10	37.499996	1
TCGTCTA	30	1.1399903E-4	37.499996	19
ATGAATG	1920	0.0	37.499996	21
>>END_MODULE