##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935981.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1023267
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04097952929197	33.0	31.0	34.0	30.0	34.0
2	32.20520939305186	33.0	31.0	34.0	30.0	34.0
3	32.00540425910344	33.0	31.0	34.0	30.0	34.0
4	35.73365602526027	37.0	35.0	37.0	33.0	37.0
5	32.033110615313504	37.0	35.0	37.0	0.0	37.0
6	33.84980361919225	37.0	35.0	37.0	17.0	37.0
7	35.50952292998797	37.0	35.0	37.0	32.0	37.0
8	35.74458572396061	37.0	35.0	37.0	35.0	37.0
9	37.7282732659218	39.0	38.0	39.0	35.0	39.0
10	37.37999466414924	39.0	37.0	39.0	34.0	39.0
11	37.35726550352938	39.0	37.0	39.0	34.0	39.0
12	37.374511246820234	39.0	37.0	39.0	35.0	39.0
13	37.357186345303816	39.0	37.0	39.0	34.0	39.0
14	38.67769702335754	40.0	38.0	41.0	35.0	41.0
15	38.75814034851119	40.0	38.0	41.0	35.0	41.0
16	38.72636369588778	40.0	38.0	41.0	35.0	41.0
17	38.661111909208444	40.0	38.0	41.0	34.0	41.0
18	38.4086646007347	40.0	38.0	41.0	34.0	41.0
19	38.09967877396613	40.0	37.0	41.0	34.0	41.0
20	37.90636656903819	40.0	35.0	41.0	34.0	41.0
21	37.88404883573887	40.0	35.0	41.0	34.0	41.0
22	37.90066326774928	40.0	35.0	41.0	34.0	41.0
23	37.88031862651683	40.0	35.0	41.0	34.0	41.0
24	37.83838431220786	40.0	35.0	41.0	34.0	41.0
25	37.62113505077365	39.0	35.0	41.0	33.0	41.0
26	37.62004442633252	40.0	35.0	41.0	33.0	41.0
27	37.58112691995344	40.0	35.0	41.0	33.0	41.0
28	37.473401370316836	40.0	35.0	41.0	33.0	41.0
29	37.38493863282994	40.0	35.0	41.0	33.0	41.0
30	37.15220758609434	39.0	35.0	41.0	33.0	41.0
31	36.95241222476636	39.0	35.0	41.0	32.0	41.0
32	36.523076577276505	39.0	35.0	41.0	31.0	41.0
33	36.054548812773206	39.0	35.0	41.0	27.0	41.0
34	35.6124960543045	39.0	35.0	41.0	23.0	41.0
35	35.33616739326099	39.0	35.0	41.0	21.0	41.0
36	35.11122219323011	39.0	35.0	41.0	20.0	41.0
37	35.0030656710321	39.0	35.0	41.0	18.0	41.0
38	34.88110141341409	39.0	35.0	41.0	18.0	41.0
39	34.800648315640004	39.0	35.0	41.0	18.0	41.0
40	34.698297707245516	39.0	35.0	41.0	18.0	41.0
41	34.59088194967687	39.0	35.0	41.0	18.0	41.0
42	34.52917176064507	39.0	35.0	41.0	17.0	41.0
43	34.46573670410557	39.0	35.0	41.0	15.0	41.0
44	34.43207198121311	39.0	35.0	41.0	15.0	41.0
45	34.426400929571656	39.0	35.0	41.0	17.0	41.0
46	34.311155348506304	39.0	34.0	41.0	15.0	41.0
47	34.19507127660718	38.0	34.0	41.0	15.0	41.0
48	34.143443500083556	38.0	34.0	41.0	15.0	41.0
49	34.07842039272253	38.0	34.0	41.0	15.0	41.0
50	33.96675647704851	38.0	34.0	40.0	15.0	41.0
51	33.06585573462254	37.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	19.0
9	34.0
10	33.0
11	26.0
12	15.0
13	17.0
14	39.0
15	48.0
16	99.0
17	177.0
18	432.0
19	903.0
20	1877.0
21	3279.0
22	4864.0
23	7240.0
24	11338.0
25	18146.0
26	24714.0
27	25335.0
28	22176.0
29	19493.0
30	18727.0
31	20436.0
32	24103.0
33	32217.0
34	50071.0
35	69022.0
36	70362.0
37	104587.0
38	179308.0
39	313886.0
40	240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.884898076455116	16.985498408528763	20.513414387447266	22.616189127568855
2	40.07194603168088	24.049637093739953	21.515498887387164	14.362917987192002
3	26.667331204856602	23.849102922306688	35.19658114646519	14.286984726371513
4	24.00585575416778	22.00579125487287	38.242316032863364	15.74603695809598
5	21.624268152886785	33.60423037193616	30.090484692655973	14.68101678252108
6	21.695315103487165	32.42917049020441	32.973603174928925	12.901911231379493
7	69.35355093050005	4.917191700699817	21.885978928275808	3.8432784405243203
8	70.7313926863663	6.557232862977111	19.058564382512092	3.6528100681444826
9	65.71667023367313	6.270699631669936	20.30349850039139	7.709131634265544
10	33.82704611797312	25.50644162276317	27.75082163306351	12.915690626200199
11	25.408031334930183	22.967612558599075	35.024680752921775	16.599675353548974
12	22.512012993676137	20.509896244088786	38.13638082729141	18.841709934943665
13	21.803302559351568	21.30910114368977	40.08777767679403	16.79981862016463
14	18.310470287813445	25.735707298290674	37.49979233181565	18.454030082080237
15	17.499831422297408	24.086088967981965	41.31170066072687	17.102378948993763
16	19.888357584090958	23.47520246426397	38.0098253925906	18.62661455905448
17	19.893243894311063	24.126254437991257	37.2685721322001	18.71192953549758
18	20.065535192672098	23.90871590699202	36.86721061072037	19.158538289615514
19	20.271248852938676	25.372947627549795	35.19110847901867	19.16469504049285
20	22.047227165539397	24.259748433204628	36.727266686016456	16.965757715239523
21	20.83268589722917	25.99047951316714	36.14012764996819	17.0367069396355
22	19.601433447966173	23.02996187700766	37.276487954756675	20.09211672026949
23	19.895100692194706	24.904350477441373	36.641951709573355	18.558597120790566
24	19.55687029875878	23.88682523720593	36.85587437100972	19.700430093025574
25	18.843664459031707	27.387768783709433	34.756324595633394	19.012242161625462
26	19.041950927763722	25.412331287923873	36.49018291413678	19.055534870175624
27	21.144432489272106	25.018983315205123	35.06963480694677	18.766949388576002
28	18.70049556958252	25.721536998652354	37.23163162693608	18.346335804829042
29	20.711700856179277	23.898356929325388	36.34799128673162	19.041950927763722
30	19.796494951952912	26.551623378844425	35.46904180433846	18.182839864864206
31	20.469926226488298	25.628990283083496	34.44897568278856	19.45210780763965
32	21.702155937795318	26.79632979466747	33.987805724214695	17.513708543322515
33	20.93656885250868	27.488719952856876	32.498360642921156	19.076350551713286
34	21.989373252533305	26.13501656947796	32.80903224671567	19.06657793127307
35	21.428033934447217	26.93461237389655	32.342096442082074	19.295257249574156
36	21.82871137249613	28.335224335388514	31.708048827920766	18.128015464194586
37	21.49771271818597	28.68010011072379	32.2171046266517	17.60508254443855
38	20.50784399379634	27.426956991674707	32.05419504391327	20.011003970615686
39	22.789555414178313	27.525562731916498	30.633353758110054	19.051528095795135
40	21.528594198777053	26.413438525819753	33.03302070720545	19.02494656819774
41	21.120685021602377	26.76339606378394	31.712251054710062	20.403667859903624
42	21.17013448102988	25.840176610796593	32.96021468492583	20.029474223247696
43	22.21648895156396	26.438260981737905	31.30101918658571	20.044230880112423
44	20.914971361335798	26.83757025292519	31.1792523359006	21.06820604983841
45	20.268610245419815	26.03445630514812	30.987611249067935	22.709322200364127
46	21.84483619622249	27.727367344006986	30.188113170853747	20.239683288916773
47	20.460739963274495	26.350307397775946	33.12566514897871	20.06328748997085
48	20.476180703570034	25.251083050660288	32.45086570758169	21.82187053818798
49	20.23997646752998	25.130488914428007	33.690327158014476	20.93920746002754
50	20.048237654492915	25.113875459679637	33.008589156104904	21.829297729722548
51	20.01530392360938	25.133225248151263	31.309423640164297	23.542047188075056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	561.0
1	1000.0
2	1439.0
3	20318.0
4	39197.0
5	26131.0
6	13065.0
7	12612.5
8	12160.0
9	12071.5
10	11983.0
11	11705.0
12	11427.0
13	11102.0
14	10777.0
15	10343.0
16	9909.0
17	9313.0
18	8717.0
19	8356.5
20	7996.0
21	7714.5
22	7433.0
23	7546.5
24	7660.0
25	8273.0
26	10254.5
27	11623.0
28	12851.5
29	14080.0
30	16132.5
31	18185.0
32	19950.5
33	21716.0
34	25392.0
35	29068.0
36	31527.0
37	33986.0
38	36335.5
39	38685.0
40	43074.5
41	47464.0
42	54315.0
43	61166.0
44	79015.5
45	96865.0
46	88842.5
47	80820.0
48	80362.0
49	79904.0
50	72658.0
51	65412.0
52	60218.5
53	55025.0
54	50578.0
55	46131.0
56	43148.0
57	40165.0
58	37820.0
59	35475.0
60	32714.0
61	29953.0
62	27022.5
63	24092.0
64	19758.5
65	15425.0
66	12603.0
67	9781.0
68	7879.5
69	5978.0
70	5294.5
71	4611.0
72	3681.5
73	2752.0
74	2144.5
75	1248.0
76	959.0
77	736.0
78	513.0
79	414.5
80	316.0
81	260.5
82	205.0
83	149.5
84	94.0
85	75.0
86	56.0
87	30.5
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1023267.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.81686692336423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0735200591373	28.00788664409137
2	8.81916465169106	6.493880227130952
3	3.593997324997794	3.969591637121124
4	2.0232304432689365	2.9795602394052665
5	1.3018403290909177	2.3964841075804504
6	0.9286359978515063	2.0513680771886675
7	0.726086138240846	1.8712551708558869
8	0.6100427400212775	1.7967889901542282
9	0.515308265570099	1.707483225420357
>10	5.2940433225923975	39.01581690044871
>50	0.08566498739870672	1.9994985627969863
>100	0.02101676141388967	1.297095371103684
>500	0.003990524317113417	1.031564482688364
>1k	0.002660349544742278	1.964889393012844
>5k	5.320699089484556E-4	1.253705410027636
>10k+	2.660349544742278E-4	2.163131560973464
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22085	2.1582832242220262	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	7308	0.714183101771092	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	5492	0.536712314576743	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	4718	0.46107223236945977	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2461	0.2405041890337517	No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	2387	0.23327244990799076	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2202	0.21519310209358847	TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	1620	0.15831645113152287	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	1616	0.15792554631391414	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	1325	0.12948722083288136	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	1302	0.1272395181316313	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1245	0.1216691244807074	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	1185	0.1158055522165769	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9545240880434922E-4	0.0	0.0	0.09068991768521803	0.0
2	1.9545240880434922E-4	0.0	0.0	0.4737766389417425	0.0
3	1.9545240880434922E-4	0.0	0.0	0.7646098232426141	0.0
4	1.9545240880434922E-4	0.0	0.0	1.2170821496246824	0.0
5	1.9545240880434922E-4	0.0	0.0	2.5110748221138763	0.0
6	1.9545240880434922E-4	0.0	0.0	3.423739845025785	0.0
7	1.9545240880434922E-4	0.0	0.0	4.163527212350247	0.0
8	1.9545240880434922E-4	0.0	0.0	5.0314336336459595	0.0
9	1.9545240880434922E-4	0.0	0.0	5.466608421848843	0.0
10	1.9545240880434922E-4	0.0	0.0	6.746626247108526	0.0
11	2.931786132065238E-4	0.0	0.0	7.552671981017662	0.0
12	2.931786132065238E-4	0.0	0.0	8.67877103434392	0.0
13	3.9090481760869843E-4	0.0	0.0	9.015046903691802	0.0
14	3.9090481760869843E-4	0.0	0.0	9.192810869499358	0.0
15	3.9090481760869843E-4	0.0	0.0	9.450123965690285	0.0
16	3.9090481760869843E-4	0.0	0.0	9.808485957233058	0.0
17	3.9090481760869843E-4	0.0	0.0	10.250892484561703	0.0
18	3.9090481760869843E-4	0.0	0.0	10.728675897883935	0.0
19	3.9090481760869843E-4	0.0	0.0	11.118017096222198	0.0
20	3.9090481760869843E-4	0.0	0.0	11.435627260529266	0.0
21	3.9090481760869843E-4	0.0	0.0	11.784705262653834	0.0
22	3.9090481760869843E-4	0.0	0.0	12.206687013262423	0.0
23	4.886310220108731E-4	0.0	0.0	12.600914521820796	0.0
24	4.886310220108731E-4	0.0	0.0	12.927906401750471	0.0
25	4.886310220108731E-4	0.0	0.0	13.230662182988409	0.0
26	4.886310220108731E-4	0.0	0.0	13.518270402544008	0.0
27	4.886310220108731E-4	0.0	0.0	13.822296624439174	0.0
28	5.863572264130476E-4	0.0	0.0	14.12368423881548	0.0
29	5.863572264130476E-4	0.0	0.0	14.444421641663418	0.0
30	6.840834308152222E-4	0.0	0.0	14.851842187816084	0.0
31	6.840834308152222E-4	0.0	0.0	15.203754249868314	0.0
32	6.840834308152222E-4	0.0	0.0	15.545307334253915	0.0
33	6.840834308152222E-4	0.0	0.0	15.882365013237015	0.0
34	6.840834308152222E-4	0.0	0.0	16.208672809735877	0.0
35	6.840834308152222E-4	0.0	0.0	16.55853262149566	0.0
36	6.840834308152222E-4	0.0	0.0	16.880833643614032	0.0
37	6.840834308152222E-4	0.0	0.0	17.21466635785186	0.0
38	6.840834308152222E-4	0.0	0.0	17.564526169611646	0.0
39	6.840834308152222E-4	0.0	0.0	17.926699483126104	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.032922E-4	45.000004	1
CTACGTC	20	7.032922E-4	45.000004	12
CGGTCGA	20	7.032922E-4	45.000004	41
CGTCGAA	75	0.0	45.0	42
GCGTACG	65	0.0	44.999996	1
CGTTTTT	12600	0.0	44.0	1
CGTTATT	1555	0.0	43.84244	1
GGCCGAT	110	0.0	42.954544	8
CCGTCGA	80	0.0	42.187504	41
CGAATAT	80	0.0	42.187504	14
TCGAATG	125	0.0	41.399998	44
ATGATCG	60	3.6379788E-12	41.250004	27
GTCGAAT	120	0.0	41.250004	43
TAATACG	200	0.0	40.5	4
CCGATGA	905	0.0	40.276245	18
CGATGAA	925	0.0	40.135136	19
TACGGCT	600	0.0	40.125	7
GCCGATT	40	3.457717E-7	39.375004	9
TACGAAT	80	0.0	39.375004	12
GCTACGA	80	0.0	39.375004	10
>>END_MODULE