FastQCFastQC Report
Sat 14 Jan 2017
SRR2935980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157602
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291832.520987351438577No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.2880955630691723No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC15410.13312001879747962No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT15060.13009652713108652No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC13180.11385605760874636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT138800.043.9139061
CGTTATT18400.043.7771721
CGGTCTA900.042.531
CGACGGT950.040.26315728
GCGTAAG900.040.01
CGTAAGG1850.038.918922
ACGGGTC1250.037.8000035
TAATGCG301.1399272E-437.4999961
GTTTTTT164800.037.395332
GTTATTT22450.036.982182
TCGTACG552.748493E-936.8181830
TCGTAAG552.748493E-936.818181
CGTATGG1350.036.6666642
TATGGGA6850.036.4598544
ACGGGTA1550.036.290325
ATGACGG1800.036.252
CGGCGAA250.002107115235.99999631
CCTTACG250.002107115235.99999628
CCGATCG250.002107115235.99999611
GTTAACG250.002107115235.9999961
Produced by FastQC (version 0.11.5)