##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935979.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1004853
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16456834979843	33.0	31.0	34.0	30.0	34.0
2	32.3194974787357	34.0	31.0	34.0	30.0	34.0
3	32.153162701410054	33.0	31.0	34.0	30.0	34.0
4	35.83502064481073	37.0	35.0	37.0	35.0	37.0
5	32.343005394818945	37.0	35.0	37.0	0.0	37.0
6	34.0542278323297	37.0	35.0	37.0	19.0	37.0
7	35.64574818406275	37.0	35.0	37.0	32.0	37.0
8	35.88645304338048	37.0	35.0	37.0	35.0	37.0
9	37.870169069505685	39.0	38.0	39.0	35.0	39.0
10	37.493662257066454	39.0	37.0	39.0	35.0	39.0
11	37.430813263233524	39.0	37.0	39.0	35.0	39.0
12	37.452127823671724	39.0	37.0	39.0	35.0	39.0
13	37.454387855736115	39.0	37.0	39.0	35.0	39.0
14	38.79125802480562	40.0	38.0	41.0	35.0	41.0
15	38.895141876473474	40.0	38.0	41.0	35.0	41.0
16	38.81892177263739	40.0	38.0	41.0	35.0	41.0
17	38.79280153415475	40.0	38.0	41.0	35.0	41.0
18	38.56562601693979	40.0	38.0	41.0	35.0	41.0
19	38.29623437458016	40.0	37.0	41.0	34.0	41.0
20	38.20812994537509	40.0	36.0	41.0	34.0	41.0
21	38.1233653081595	40.0	36.0	41.0	34.0	41.0
22	38.16567696966621	40.0	36.0	41.0	34.0	41.0
23	38.1544962297968	40.0	36.0	41.0	34.0	41.0
24	38.1065369760552	40.0	35.0	41.0	34.0	41.0
25	37.857688637044426	40.0	35.0	41.0	34.0	41.0
26	37.88864241834378	40.0	35.0	41.0	34.0	41.0
27	37.87555692225629	40.0	35.0	41.0	34.0	41.0
28	37.788523296442364	40.0	35.0	41.0	34.0	41.0
29	37.72314258901551	40.0	35.0	41.0	33.0	41.0
30	37.49933472856229	40.0	35.0	41.0	33.0	41.0
31	37.35304765970744	40.0	35.0	41.0	33.0	41.0
32	37.04552705719145	40.0	35.0	41.0	32.0	41.0
33	36.683443249908194	40.0	35.0	41.0	31.0	41.0
34	36.33851319546242	40.0	35.0	41.0	29.0	41.0
35	36.14562627568411	40.0	35.0	41.0	27.0	41.0
36	35.9562771868124	40.0	35.0	41.0	25.0	41.0
37	35.88785324818655	40.0	35.0	41.0	25.0	41.0
38	35.73610965982089	40.0	35.0	41.0	24.0	41.0
39	35.6317361842976	39.0	35.0	41.0	23.0	41.0
40	35.555996747783006	39.0	35.0	41.0	23.0	41.0
41	35.42547417383438	39.0	35.0	41.0	23.0	41.0
42	35.389007148309254	39.0	35.0	41.0	23.0	41.0
43	35.304415670749854	39.0	35.0	41.0	22.0	41.0
44	35.24195081270594	39.0	35.0	41.0	22.0	41.0
45	35.203185938639784	39.0	35.0	41.0	22.0	41.0
46	35.08324899263872	39.0	35.0	41.0	22.0	41.0
47	34.986683624370926	39.0	35.0	41.0	22.0	41.0
48	34.93073713269503	39.0	35.0	41.0	22.0	41.0
49	34.86467672385911	39.0	35.0	41.0	20.0	41.0
50	34.75159650217495	38.0	35.0	41.0	20.0	41.0
51	33.851185198232976	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	17.0
9	28.0
10	38.0
11	32.0
12	20.0
13	23.0
14	28.0
15	49.0
16	86.0
17	166.0
18	303.0
19	680.0
20	1163.0
21	2003.0
22	3270.0
23	5183.0
24	8701.0
25	13688.0
26	19420.0
27	20518.0
28	18695.0
29	16648.0
30	15876.0
31	17739.0
32	21194.0
33	29473.0
34	48665.0
35	66821.0
36	69145.0
37	106118.0
38	183521.0
39	335273.0
40	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.50817283722097	19.061295532779422	21.900815343139744	21.529716286859866
2	38.276942000471706	23.3353535293222	22.87677899155399	15.510925478652101
3	29.491477857955346	22.462887606445918	32.72359240605342	15.322042129545316
4	25.557270565943472	23.48044937916292	33.78414554168619	17.178134513207404
5	21.445823418947846	34.321836129264675	28.49352094286428	15.738819508923196
6	23.552400201820564	31.977811679917362	30.679114258503482	13.790673859758593
7	74.27036591421829	5.133288152595454	16.666815942232347	3.9295299909539008
8	76.68146485107772	5.075468750155496	15.041503583111163	3.201562815655623
9	70.96928605477618	7.026301359502335	16.531671796770272	5.47274078895122
10	34.774439644405696	29.204371186631278	23.513688071787612	12.507501097175409
11	25.641362467943075	24.229713201831512	33.34049856048596	16.788425769739455
12	23.43775656737851	22.01108022765519	35.85589135923364	18.69527184573266
13	21.751937845635133	22.593653001981384	37.4364210486509	18.217988103732587
14	19.01233314723646	26.11347132366625	35.75030377577616	19.123891753321132
15	18.191317535997804	24.890406855530113	38.49587949680202	18.422396111670064
16	21.130254873100842	24.662711859346594	36.38134135042638	17.82569191712619
17	21.139310924085414	24.099345874471194	34.8017073144032	19.959635887040193
18	21.696407335202263	23.978333149226803	35.09986037758757	19.225399137983366
19	21.91345400770063	25.552294713754154	33.59794915276165	18.936302125783573
20	23.6177828995883	24.773374812037183	34.12648417231177	17.482358116062745
21	22.650875302158624	25.452578635880073	34.29735493649319	17.599191125468103
22	21.846777588363672	23.14089722576337	34.58466064190483	20.42766454396812
23	20.661231045735047	25.74665150026919	34.389408201995714	19.202709252000044
24	19.972971170907584	24.820645407835773	35.585105483090565	19.621277938166077
25	20.467172810351364	26.462378079181732	33.88386161956027	19.186587490906632
26	20.111399378814614	26.266727571097466	34.133848433551975	19.488024616535952
27	21.351182710306883	25.462132272083576	33.76832233172414	19.4183626858854
28	19.55261117795339	26.493128845711762	34.92879057931857	19.02546939701628
29	19.95316727919407	24.510948367572173	35.4204047756239	20.11547957760986
30	20.394624885431003	24.446859391373664	34.71662024196574	20.441895481229594
31	21.569423587330686	24.89359140093128	32.634226100733144	20.90275891100489
32	21.87832449124399	25.538262810580253	32.76618570079405	19.817226997381706
33	20.895295132720907	25.687836927391373	32.35816582126938	21.058702118618346
34	20.71497024937976	26.84193608418346	32.544760278369075	19.89833338806771
35	21.094130186206343	26.055850955313865	31.686425775710475	21.16359308276932
36	21.152845242040378	27.852531663835407	30.934474992859652	20.060148101264563
37	21.449007964349015	27.375148404791545	30.766191671816674	20.409651959042765
38	21.04924799945863	27.104860113867403	30.816845847104002	21.02904603956997
39	21.529716286859866	26.529751117825196	30.997270247488935	20.943262347826
40	21.985902415577204	26.16770811252989	32.18530471621222	19.661084755680683
41	20.335909829596964	26.973497616069213	31.926858953498673	20.763733600835145
42	21.82995920796375	26.744707932404044	31.104947688865934	20.320385170766272
43	21.712728130383248	26.575230406835626	30.992393912343395	20.719647550437724
44	21.82747128186909	26.499796487645455	30.198347419970883	21.474384810514575
45	21.085173652265553	26.060329222284256	30.26790983357765	22.586587291872544
46	21.33356819355667	26.86502403834193	29.93174125966684	21.86966650843457
47	20.918283569835587	26.946528497203076	31.507693165069917	20.62749476789142
48	21.393477453916145	25.71460701216994	31.229642544730424	21.662272989183492
49	21.1084606405116	25.802779112964778	31.803159268072044	21.285600978451573
50	20.016858187217434	26.04261518849026	31.63796097538645	22.30256564890586
51	20.268238239822143	25.517165197297516	30.72668340543343	23.48791315744691
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	598.0
1	1127.5
2	1657.0
3	15903.5
4	30150.0
5	20217.5
6	10285.0
7	10053.5
8	9822.0
9	9679.0
10	9536.0
11	9357.0
12	9178.0
13	8856.5
14	8535.0
15	8137.5
16	7740.0
17	7607.5
18	7475.0
19	7312.0
20	7149.0
21	6941.5
22	6734.0
23	7072.0
24	7410.0
25	8410.5
26	10621.5
27	11832.0
28	13494.5
29	15157.0
30	16689.0
31	18221.0
32	20986.5
33	23752.0
34	26681.0
35	29610.0
36	32619.5
37	35629.0
38	38508.0
39	41387.0
40	44633.0
41	47879.0
42	51505.5
43	55132.0
44	58848.0
45	62564.0
46	70197.0
47	77830.0
48	77010.0
49	76190.0
50	74530.0
51	72870.0
52	67117.5
53	61365.0
54	56609.5
55	51854.0
56	48513.5
57	45173.0
58	41313.0
59	37453.0
60	35276.0
61	33099.0
62	30016.0
63	26933.0
64	22794.5
65	18656.0
66	15361.5
67	12067.0
68	10013.0
69	7959.0
70	6924.0
71	5889.0
72	5251.0
73	4613.0
74	3647.0
75	2222.0
76	1763.0
77	1375.5
78	988.0
79	681.0
80	374.0
81	255.5
82	137.0
83	93.5
84	50.0
85	33.5
86	17.0
87	27.0
88	37.0
89	21.0
90	5.0
91	4.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1004853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.828931812630074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.59728520183616	25.23546474500234
2	8.62343394806013	5.834431180392909
3	3.7210700480143064	3.7763947477288826
4	2.128846338535313	2.880663905035132
5	1.3593853515208967	2.2993277181844287
6	1.0162948573384987	2.062810165825841
7	0.7743910616306674	1.8337775694149843
8	0.6514636228654886	1.7630654781060455
9	0.5327097146592517	1.6218900551820101
>10	6.438713528484106	45.237913547199746
>50	0.12417915249632626	2.5888197158838997
>100	0.02779224228019727	1.6790247909565146
>500	0.002365297215263293	0.5857133005099788
>1k	0.0017739729114474698	0.9606858352797722
>5k	0.0	0.0
>10k+	2.956621519079116E-4	1.6400172452974937
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16397	1.6317809669673076	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	2391	0.23794525169353128	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	1821	0.18122053673522398	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1796	0.17873261064056137	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	1414	0.1407170999141168	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	1096	0.10907067999000848	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT	1087	0.10817502659592995	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.985511313595123E-4	0.0	0.0	0.054336305907431236	0.0
2	2.985511313595123E-4	0.0	0.0	0.25894334793248364	0.0
3	2.985511313595123E-4	0.0	0.0	0.4567832309800538	0.0
4	2.985511313595123E-4	0.0	0.0	0.6821893351564856	0.0
5	2.985511313595123E-4	0.0	0.0	1.3812965677566769	0.0
6	2.985511313595123E-4	0.0	0.0	2.050648204264703	0.0
7	2.985511313595123E-4	0.0	0.0	2.5619667752397612	0.0
8	2.985511313595123E-4	0.0	0.0	3.2020604008745557	0.0
9	2.985511313595123E-4	0.0	0.0	3.5541517017912074	0.0
10	2.985511313595123E-4	0.0	0.0	4.320831007122435	0.0
11	2.985511313595123E-4	0.0	0.0	5.0083942626433915	0.0
12	2.985511313595123E-4	0.0	0.0	5.722429051811559	0.0
13	2.985511313595123E-4	0.0	0.0	6.031628506856227	0.0
14	2.985511313595123E-4	0.0	0.0	6.184188134980937	0.0
15	2.985511313595123E-4	0.0	0.0	6.376654097664036	0.0
16	2.985511313595123E-4	0.0	0.0	6.669532757527718	0.0
17	3.980681751460164E-4	0.0	0.0	7.0261023254147625	0.0
18	3.980681751460164E-4	0.0	0.0	7.421782091509902	0.0
19	3.980681751460164E-4	0.0	0.0	7.702121603856485	9.95170437865041E-5
20	3.980681751460164E-4	0.0	0.0	7.9552929632493505	9.95170437865041E-5
21	3.980681751460164E-4	0.0	0.0	8.250958100339055	9.95170437865041E-5
22	3.980681751460164E-4	0.0	0.0	8.58274792432326	9.95170437865041E-5
23	4.975852189325205E-4	0.0	0.0	8.919911668671935	9.95170437865041E-5
24	4.975852189325205E-4	0.0	0.0	9.185522658538115	9.95170437865041E-5
25	4.975852189325205E-4	0.0	0.0	9.437798364536903	9.95170437865041E-5
26	4.975852189325205E-4	0.0	0.0	9.67385279239849	9.95170437865041E-5
27	4.975852189325205E-4	0.0	0.0	9.923242504127469	9.95170437865041E-5
28	4.975852189325205E-4	0.0	0.0	10.194426448445693	9.95170437865041E-5
29	4.975852189325205E-4	0.0	0.0	10.470586244953243	9.95170437865041E-5
30	4.975852189325205E-4	0.0	0.0	10.801380898499582	9.95170437865041E-5
31	4.975852189325205E-4	0.0	0.0	11.103116575260263	9.95170437865041E-5
32	4.975852189325205E-4	0.0	0.0	11.401170121400842	9.95170437865041E-5
33	5.971022627190246E-4	0.0	0.0	11.68131059965985	9.95170437865041E-5
34	5.971022627190246E-4	0.0	0.0	11.952096475802929	9.95170437865041E-5
35	5.971022627190246E-4	0.0	0.0	12.24497513566661	9.95170437865041E-5
36	5.971022627190246E-4	0.0	0.0	12.524020926443967	9.95170437865041E-5
37	5.971022627190246E-4	0.0	0.0	12.812520836381044	9.95170437865041E-5
38	5.971022627190246E-4	0.0	0.0	13.09614441117258	9.95170437865041E-5
39	5.971022627190246E-4	0.0	0.0	13.405244349173461	9.95170437865041E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTCG	20	7.0328615E-4	45.000004	10
CCGCGAA	20	7.0328615E-4	45.000004	33
ACGACTA	20	7.0328615E-4	45.000004	19
CGTTTTT	9325	0.0	43.455765	1
CGTTATT	1240	0.0	43.004032	1
TATTGCG	55	6.002665E-11	40.909092	1
TAATACG	100	0.0	40.500004	4
TCAAGCG	240	0.0	40.312504	17
TAGTACG	45	1.9279469E-8	40.000004	1
CCCGATG	45	1.9279469E-8	40.000004	15
CGAATGC	80	0.0	39.375004	45
CGTAAGG	160	0.0	39.375004	2
TACTACG	40	3.4576442E-7	39.375004	1
ACGTAAG	40	3.4576442E-7	39.375004	1
TACGGGA	375	0.0	39.0	4
CGCTCGA	35	6.2475046E-6	38.57143	41
ACTACGG	70	0.0	38.57143	2
GCGAGAC	260	0.0	38.07692	21
CACGTGA	220	0.0	37.84091	43
CGAACGG	60	1.546141E-10	37.500004	2
>>END_MODULE