##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935977.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413471
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.977650185865514	33.0	31.0	34.0	30.0	34.0
2	32.10265532528279	33.0	31.0	34.0	30.0	34.0
3	31.98845142706502	33.0	31.0	34.0	30.0	34.0
4	35.68440591964128	37.0	35.0	37.0	33.0	37.0
5	31.8138418413867	37.0	35.0	37.0	0.0	37.0
6	33.726157336306535	37.0	35.0	37.0	17.0	37.0
7	35.468431885186625	37.0	35.0	37.0	32.0	37.0
8	35.74759293880345	37.0	35.0	37.0	35.0	37.0
9	37.733197733335594	39.0	37.0	39.0	35.0	39.0
10	37.35918117594704	39.0	37.0	39.0	34.0	39.0
11	37.303486822534104	39.0	37.0	39.0	34.0	39.0
12	37.211066797913276	39.0	37.0	39.0	34.0	39.0
13	37.08151962289979	39.0	37.0	39.0	33.0	39.0
14	38.291887460063705	40.0	38.0	41.0	33.0	41.0
15	38.465270841244006	40.0	38.0	41.0	34.0	41.0
16	38.42837829013401	40.0	37.0	41.0	34.0	41.0
17	38.4356847275867	40.0	37.0	41.0	34.0	41.0
18	38.26836706806523	40.0	37.0	41.0	34.0	41.0
19	38.04392327394182	40.0	37.0	41.0	34.0	41.0
20	37.99933973603953	40.0	35.0	41.0	34.0	41.0
21	37.89734467471721	40.0	35.0	41.0	34.0	41.0
22	37.958703270604225	40.0	35.0	41.0	34.0	41.0
23	37.95998268318697	40.0	35.0	41.0	34.0	41.0
24	37.892243954231375	40.0	35.0	41.0	34.0	41.0
25	37.658113870138415	39.0	35.0	41.0	33.0	41.0
26	37.705652875292344	40.0	35.0	41.0	34.0	41.0
27	37.66497529451884	40.0	35.0	41.0	34.0	41.0
28	37.60620696493829	40.0	35.0	41.0	33.0	41.0
29	37.56224741275688	40.0	35.0	41.0	33.0	41.0
30	37.31823755474991	39.0	35.0	41.0	33.0	41.0
31	37.2386019817593	39.0	35.0	41.0	33.0	41.0
32	37.024054891395046	39.0	35.0	41.0	33.0	41.0
33	36.72592273702388	39.0	35.0	41.0	31.0	41.0
34	36.518938450338716	39.0	35.0	41.0	31.0	41.0
35	36.42138142699246	39.0	35.0	41.0	30.0	41.0
36	36.26156852596675	39.0	35.0	41.0	30.0	41.0
37	36.19722302168713	39.0	35.0	41.0	30.0	41.0
38	36.05283804668284	39.0	35.0	41.0	29.0	41.0
39	35.989534937154	39.0	35.0	41.0	29.0	41.0
40	35.90581927148458	39.0	35.0	41.0	28.0	41.0
41	35.834711503346064	39.0	35.0	41.0	28.0	41.0
42	35.8156484977181	39.0	35.0	41.0	27.0	41.0
43	35.72684904140798	39.0	35.0	41.0	27.0	41.0
44	35.63498044602886	39.0	35.0	41.0	27.0	41.0
45	35.600999828283	39.0	35.0	41.0	26.0	41.0
46	35.448490946160675	39.0	35.0	41.0	25.0	41.0
47	35.39637846426956	39.0	35.0	41.0	26.0	41.0
48	35.371714098449466	38.0	35.0	41.0	26.0	41.0
49	35.36282351120151	38.0	35.0	41.0	26.0	41.0
50	35.2481431587705	38.0	35.0	40.0	25.0	41.0
51	34.31809244179156	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	3.0
9	11.0
10	9.0
11	6.0
12	9.0
13	5.0
14	6.0
15	9.0
16	16.0
17	62.0
18	131.0
19	235.0
20	417.0
21	722.0
22	1165.0
23	1808.0
24	2680.0
25	4371.0
26	5971.0
27	6919.0
28	6654.0
29	6516.0
30	6923.0
31	8057.0
32	10222.0
33	15052.0
34	25047.0
35	36410.0
36	30225.0
37	45126.0
38	75153.0
39	123446.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.334146772083166	18.191118603239406	23.6418031736204	17.83293145105703
2	35.94157752297017	23.116736119340896	24.311983186245225	16.629703171443705
3	29.71598975502514	23.144549436357085	32.54279018359208	14.596670625025698
4	26.079942728752442	23.988139434204577	32.34567841517301	17.586239421869973
5	21.889564201600596	35.80444577733384	26.689175298872232	15.616814722193334
6	22.723963712086217	32.9113770977892	29.774760503154994	14.589898686969583
7	73.953433251667	5.791216312631358	15.90558950930053	4.349760926401126
8	75.13610386218139	5.851196335414092	14.2406601672185	4.772039635186022
9	69.49266091213168	7.347310935954396	16.62946131651313	6.530566835400791
10	36.78589308560939	27.93110036737764	22.655035056872187	12.627971490140782
11	28.009703219814693	23.77458153050637	31.457345255169045	16.75836999450989
12	25.713290653999916	20.979947807705983	34.64281654577951	18.66394499251459
13	22.58925051575564	24.733052620377247	35.57395802849535	17.103738835371768
14	17.36929554914371	28.76574173279384	33.950869589402885	19.91409312865957
15	16.896469159868527	24.604385797311057	40.94531418164756	17.55383086117285
16	19.35661751368294	23.057723516280465	38.03386452737919	19.551794442657407
17	19.05018731664373	23.644947287717883	33.66330407694856	23.641561318689824
18	19.84395519879266	23.807957510925796	36.327094282307584	20.02099300797396
19	21.03170476284915	25.514969610927974	33.27367578379137	20.179649842431512
20	23.281923036923992	24.426380568407456	34.09162915899785	18.2000672356707
21	20.965678366802024	26.349369121413595	33.69377779820108	18.9911747135833
22	19.353231544654886	23.644947287717883	33.50247054811583	23.499350619511404
23	18.589937383758475	26.340178634051725	33.51383772985288	21.556046252336923
24	19.847583022751294	23.94291256218695	35.326782289447145	20.882722125614613
25	18.09582776059264	26.35807589891431	33.31769338115612	22.22840295933693
26	17.600267007843357	27.301793838020078	33.42749551963741	21.670443634499154
27	19.615160434468198	27.22585138981936	32.94330194862518	20.215686227087268
28	17.966435372734725	25.203702315277248	36.11305266874823	20.71680964323979
29	19.45650360001064	23.509508526595578	34.56905079195397	22.46493708143981
30	19.75398516461856	26.382503246902445	33.30390765011331	20.55960393836569
31	22.191157300028298	25.920560329503157	29.769923404543487	22.118358965925058
32	22.191399154958873	26.92329087166935	29.619731492656076	21.265578480715696
33	20.744864815186556	27.03526970452583	29.57111865161039	22.648746828677222
34	19.693521431974673	25.5374621194715	30.454856567933426	24.314159880620405
35	18.8912886272556	25.38001455966682	30.901078914845296	24.827617898232283
36	21.098214868757424	27.122095624602448	29.25912579116794	22.520563715472186
37	20.929883837076847	26.831627852981228	30.980891041935227	21.257597268006702
38	20.325004655707414	27.859269452996706	29.387067049442408	22.42865884185348
39	20.47084317884447	24.97103787206358	30.570946934609683	23.987172014482272
40	20.730111664421447	24.642115166480842	31.656391863032717	22.971381306065
41	17.355026108239755	25.186530615206387	30.90712528810969	26.551317988444172
42	20.386435808073603	24.4002602359053	30.753547407194215	24.45975654882688
43	22.136256230787648	24.867040251916094	29.261060630612544	23.73564288668371
44	20.785012733662096	26.3958052680841	29.217526743108944	23.60165525514486
45	20.392724036268564	25.963852362076178	28.70745469452513	24.93596890713013
46	21.491470985873253	26.830418578328345	28.963820920935206	22.714289514863196
47	19.612500030231867	24.96233109456286	31.68759114907696	23.737577726128315
48	19.688200623502013	23.76103765439414	31.758938353596744	24.791823368507103
49	20.616197992120366	22.598682858048086	32.33261824892193	24.452500900909616
50	19.639829637386903	23.76152136425529	31.222987827441344	25.37566117091646
51	18.64169433890164	24.617687818492712	29.02283352399564	27.71778431861001
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	359.0
1	531.0
2	703.0
3	5931.5
4	11160.0
5	7192.0
6	3224.0
7	3184.0
8	3144.0
9	3099.0
10	3054.0
11	3008.5
12	2963.0
13	2879.0
14	2795.0
15	2700.5
16	2606.0
17	2375.5
18	2145.0
19	2077.0
20	2009.0
21	2051.5
22	2094.0
23	2160.5
24	2227.0
25	2592.5
26	3802.0
27	4646.0
28	5338.0
29	6030.0
30	6774.5
31	7519.0
32	8219.5
33	8920.0
34	9783.0
35	10646.0
36	11447.5
37	12249.0
38	13046.5
39	13844.0
40	16216.0
41	18588.0
42	20680.0
43	22772.0
44	24929.0
45	27086.0
46	28801.0
47	30516.0
48	34459.5
49	38403.0
50	39212.0
51	40021.0
52	38011.5
53	36002.0
54	29910.5
55	23819.0
56	20931.0
57	18043.0
58	16471.5
59	14900.0
60	13016.0
61	11132.0
62	10150.5
63	9169.0
64	7690.0
65	6211.0
66	5052.5
67	3894.0
68	3243.0
69	2592.0
70	2223.0
71	1854.0
72	1569.0
73	1284.0
74	1047.5
75	652.0
76	493.0
77	371.5
78	250.0
79	179.5
80	109.0
81	117.0
82	125.0
83	106.5
84	88.0
85	50.5
86	13.0
87	6.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	413471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.81503306382581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68228143882631	26.747483790135085
2	10.503753920829864	7.523845879376231
3	3.937970314830759	4.231156110900844
4	2.043705260724981	2.9278148594231976
5	1.2690954260342386	2.2726347322283176
6	0.8739451989375983	1.8780225717553138
7	0.6273996204922435	1.5729236706513026
8	0.5053849336948986	1.4480302488193768
9	0.4042864679022245	1.303157989365797
>10	4.878860779914575	39.30352253434606
>50	0.22901344215696917	5.111680851815055
>100	0.03748731940069436	2.0559014390633426
>500	0.0034079381273358503	0.7835957752782391
>1k	0.00272635050186868	1.2176248370990208
>5k	6.8158762546717E-4	1.622604709742821
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6647	1.6076097235356288	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1686	0.4077674129503641	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	1152	0.2786168800230246	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	1110	0.2684589729388518	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1040	0.25152912779856385	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.18767942612662072	No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	664	0.16059167390216	TruSeq Adapter, Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	641	0.15502901049892254	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC	590	0.14269440903956987	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	539	0.13035980758021723	TruSeq Adapter, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16808917675000182	0.0
2	0.0	0.0	0.0	0.9809635984143991	0.0
3	0.0	0.0	0.0	1.2992446870518126	0.0
4	0.0	0.0	0.0	1.7362765466018173	0.0
5	0.0	0.0	0.0	3.0582555971277308	0.0
6	0.0	0.0	0.0	3.8529908989989625	0.0
7	0.0	0.0	0.0	4.6931949278183955	0.0
8	0.0	0.0	0.0	6.12908765064539	0.0
9	0.0	0.0	0.0	6.6950281881921585	0.0
10	0.0	0.0	0.0	8.160427212549369	0.0
11	0.0	0.0	0.0	9.742158458513414	0.0
12	0.0	0.0	0.0	11.246012416832135	0.0
13	0.0	0.0	0.0	11.726336308955162	0.0
14	0.0	0.0	0.0	11.925382916818833	0.0
15	0.0	0.0	0.0	12.290583861987903	0.0
16	0.0	0.0	0.0	13.004297762116327	0.0
17	0.0	0.0	0.0	13.882956724897273	0.0
18	0.0	0.0	0.0	14.904310096717786	0.0
19	0.0	0.0	0.0	15.499998790725346	0.0
20	0.0	0.0	0.0	16.0819017536901	0.0
21	0.0	0.0	0.0	16.763690802982556	0.0
22	0.0	0.0	0.0	17.465070101651627	0.0
23	0.0	0.0	0.0	18.224736438589407	0.0
24	0.0	0.0	0.0	18.818248438221787	0.0
25	0.0	0.0	0.0	19.323241533263516	0.0
26	0.0	0.0	0.0	19.803565425386545	0.0
27	0.0	0.0	0.0	20.271312861119643	0.0
28	0.0	0.0	0.0	20.779208215328282	0.0
29	0.0	0.0	0.0	21.29145695828728	0.0
30	0.0	0.0	0.0	21.88448524805851	0.0
31	0.0	0.0	0.0	22.415840530532975	0.0
32	0.0	0.0	0.0	22.90777345932363	0.0
33	0.0	0.0	0.0	23.393418159919317	0.0
34	0.0	0.0	0.0	23.882206974612487	0.0
35	0.0	0.0	0.0	24.41477153173983	0.0
36	0.0	0.0	0.0	24.93355035782437	0.0
37	0.0	0.0	0.0	25.432497079601713	0.0
38	0.0	0.0	0.0	25.913546536516467	0.0
39	0.0	0.0	0.0	26.41346067801611	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTG	30	2.162118E-6	45.000004	45
TAGCCGT	35	1.2094097E-7	45.000004	44
TACGCGG	35	1.2094097E-7	45.000004	2
TGTTACG	30	2.162118E-6	45.000004	1
AACGAGC	35	1.2094097E-7	45.000004	15
CGTGTAC	35	1.2094097E-7	45.000004	18
ATCTCGC	20	7.0280425E-4	45.0	45
CCCTCGA	20	7.0280425E-4	45.0	41
GATCGGC	20	7.0280425E-4	45.0	24
TTAGCCG	20	7.0280425E-4	45.0	1
CACATCG	25	3.8864586E-5	45.0	17
GGCGTAA	20	7.0280425E-4	45.0	12
CGACCGT	20	7.0280425E-4	45.0	8
CCTCGAA	20	7.0280425E-4	45.0	42
GTTAACG	20	7.0280425E-4	45.0	1
CTACGCG	20	7.0280425E-4	45.0	1
CGAATAT	45	3.8380676E-10	45.0	14
CATCGTG	20	7.0280425E-4	45.0	33
TGTAGCG	20	7.0280425E-4	45.0	1
CAGTCGT	20	7.0280425E-4	45.0	18
>>END_MODULE