SRR2935976.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935976.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521443
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5027	0.9640555151761553	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1237	0.23722631236779476	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1201	0.23032239381869157	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG	843	0.1616667593581657	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC	820	0.1572559225073498	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	755	0.14479051401591353	No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	688	0.13194155449397152	Illumina Single End Adapter 1 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	676	0.12964024831093715	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	631	0.12101035012455821	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	552	0.1058600844195818	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGT	30	2.1632113E-6	45.000004	4
CGAACAC	20	7.0297363E-4	45.0	34
CGTTCTA	20	7.0297363E-4	45.0	18
TCGTGCG	25	3.8878643E-5	45.0	1
TTGACGA	25	3.8878643E-5	45.0	2
GCGATAT	40	6.8030204E-9	45.0	9
CATCGTT	25	3.8878643E-5	45.0	36
TAGATCG	20	7.0297363E-4	45.0	1
ACCCGTA	20	7.0297363E-4	45.0	15
ACGTAAG	20	7.0297363E-4	45.0	1
TACCGGC	20	7.0297363E-4	45.0	12
TCTATAC	25	3.8878643E-5	45.0	14
TCAAGCG	95	0.0	42.63158	17
CGTTATT	370	0.0	42.56757	1
CGTTTTT	2310	0.0	42.272728	1
CGGTAGT	80	0.0	42.1875	12
CGTTAGG	70	0.0	41.785713	2
GCGAGAC	105	0.0	40.714287	21
ACACGAC	100	0.0	40.5	26
TTGCACG	50	1.0786607E-9	40.5	1