##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935976.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521443
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12410560694074	33.0	31.0	34.0	30.0	34.0
2	32.25105524477268	34.0	31.0	34.0	30.0	34.0
3	32.169717495488484	33.0	31.0	34.0	30.0	34.0
4	35.80418569239591	37.0	35.0	37.0	35.0	37.0
5	32.14757701225254	37.0	35.0	37.0	0.0	37.0
6	33.932999388236105	37.0	35.0	37.0	17.0	37.0
7	35.57941711749894	37.0	35.0	37.0	32.0	37.0
8	35.826030841338365	37.0	35.0	37.0	35.0	37.0
9	37.81162274687742	39.0	38.0	39.0	35.0	39.0
10	37.4496963234716	39.0	37.0	39.0	35.0	39.0
11	37.35353432685835	39.0	37.0	39.0	34.0	39.0
12	37.28298586806228	39.0	37.0	39.0	35.0	39.0
13	37.198644914209225	39.0	37.0	39.0	34.0	39.0
14	38.4475752862729	40.0	38.0	41.0	34.0	41.0
15	38.59934067577856	40.0	38.0	41.0	35.0	41.0
16	38.54513724414749	40.0	38.0	41.0	35.0	41.0
17	38.54328469267015	40.0	38.0	41.0	35.0	41.0
18	38.406199718857096	40.0	38.0	41.0	34.0	41.0
19	38.21856463697854	40.0	37.0	41.0	34.0	41.0
20	38.238181354433756	40.0	36.0	41.0	34.0	41.0
21	38.149947357621066	40.0	36.0	41.0	34.0	41.0
22	38.206998655653635	40.0	36.0	41.0	34.0	41.0
23	38.21669482570482	40.0	36.0	41.0	34.0	41.0
24	38.17785453060066	40.0	36.0	41.0	34.0	41.0
25	37.92312678471089	40.0	35.0	41.0	34.0	41.0
26	37.97186077864695	40.0	36.0	41.0	34.0	41.0
27	37.93635354199788	40.0	35.0	41.0	34.0	41.0
28	37.880882474210985	40.0	36.0	41.0	34.0	41.0
29	37.849791827678196	40.0	36.0	41.0	34.0	41.0
30	37.64951490383417	40.0	35.0	41.0	33.0	41.0
31	37.58033763997216	40.0	35.0	41.0	33.0	41.0
32	37.414058679472156	40.0	35.0	41.0	33.0	41.0
33	37.237057549914375	40.0	35.0	41.0	33.0	41.0
34	37.08781976169975	40.0	35.0	41.0	32.0	41.0
35	36.99419687290845	40.0	35.0	41.0	32.0	41.0
36	36.850493726064016	40.0	35.0	41.0	31.0	41.0
37	36.75579114112185	40.0	35.0	41.0	31.0	41.0
38	36.648391099314786	40.0	35.0	41.0	31.0	41.0
39	36.57596707597954	39.0	35.0	41.0	31.0	41.0
40	36.48676653056997	39.0	35.0	41.0	30.0	41.0
41	36.38427018868793	39.0	35.0	41.0	30.0	41.0
42	36.35709368042144	39.0	35.0	41.0	30.0	41.0
43	36.278891460811636	39.0	35.0	41.0	30.0	41.0
44	36.22823779396789	39.0	35.0	41.0	30.0	41.0
45	36.19508364289098	39.0	35.0	41.0	30.0	41.0
46	36.08896657928096	39.0	35.0	41.0	30.0	41.0
47	36.00480781216739	39.0	35.0	41.0	29.0	41.0
48	35.961019708769705	39.0	35.0	41.0	29.0	41.0
49	35.93203475739438	39.0	35.0	41.0	29.0	41.0
50	35.82746915770276	39.0	35.0	41.0	29.0	41.0
51	34.85456128474253	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	22.0
10	22.0
11	12.0
12	14.0
13	10.0
14	12.0
15	20.0
16	34.0
17	83.0
18	139.0
19	236.0
20	468.0
21	782.0
22	1148.0
23	1807.0
24	2710.0
25	4132.0
26	5809.0
27	6656.0
28	6583.0
29	6831.0
30	7573.0
31	9212.0
32	11997.0
33	17317.0
34	30525.0
35	43208.0
36	37739.0
37	57776.0
38	97410.0
39	171020.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.553343318445165	19.048294828006128	22.64619526966514	16.752166583883568
2	33.54709910766853	23.802218075609417	24.97511712689594	17.67556568982612
3	31.067058144418468	23.65876999020027	29.682438924292782	15.591732941088479
4	27.371543965495746	24.466528460445343	29.53247047136504	18.62945710269387
5	22.538800981123536	36.10634335871802	24.923145962262414	16.43170969789603
6	25.17456366275892	33.67808178458623	26.877913789234874	14.269440763419972
7	77.15186511277359	6.253416001365442	12.25771560841741	4.337003277443556
8	78.61357809003093	6.042846485617795	10.950190145423374	4.393385278927899
9	73.19553623310698	7.4522047472111055	13.168265754838016	6.183993264843904
10	39.437484058660296	27.759314057337043	20.01081613906026	12.7923857449424
11	30.088235914567846	24.298916660114337	28.205192130299956	17.40765529501786
12	27.279108167143868	22.226782217807123	31.366419723728196	19.127689891320816
13	23.04163638211655	24.885557961272852	32.81010580255177	19.26269985405883
14	19.873696645654462	28.19080896665599	31.69397230378009	20.24152208390946
15	19.00629599016575	25.04990957784456	37.121602936466694	18.822191495523
16	21.417681318955285	23.815258810646608	34.58978258409836	20.17727728629975
17	21.88312049447399	23.86339446497508	31.276860558105103	22.97662448244583
18	22.153332195465275	24.64833164890506	32.56655089818062	20.63178525744904
19	23.563074008089092	25.471048609339853	30.429596331717946	20.536281050853113
20	24.6143873827053	25.682193451633257	30.49211514969038	19.211304015971066
21	23.83405281114139	25.924597702912877	30.918240344582248	19.323109141363485
22	21.72663167402765	24.54880015648882	30.87758393534864	22.84698423413489
23	21.071718289439115	26.07725101305416	30.966567774425968	21.884462923080758
24	21.722796163722595	24.697042629779286	32.84596782390405	20.73419338259407
25	20.79345201680721	26.78988882773381	30.526826517951143	21.88983263750784
26	20.701591545001083	27.290806473574293	30.304558695773075	21.703043285651546
27	20.921366285480865	26.401159858316248	31.630302832716133	21.047171023486747
28	19.647017986625574	26.32099769294055	33.30699616257194	20.724988157861933
29	21.763069021925695	23.667783439417157	32.63693251227843	21.93221502637872
30	22.259384055400112	24.826874653605476	32.46433454855085	20.449406742443564
31	24.398448152530573	25.65918038980291	28.80986032989224	21.132511127774272
32	24.318285987154876	26.375845490302872	29.334366364108828	19.971502158433424
33	23.590306131254998	26.34496963234716	29.26340942346527	20.801314812932574
34	22.69145429126482	26.1031407076133	29.50715610335166	21.698248897770224
35	23.14615403792936	25.36077768806945	29.994457687609195	21.49861058639199
36	24.967829657316333	27.815696058821388	28.250067600869126	18.966406682993156
37	22.481843653093435	27.854242937387212	30.186041427346805	19.477871982172548
38	23.480418761015105	26.72852066285289	28.81120275849901	20.97985781763299
39	23.514363027214863	26.79142303185583	29.085249969795356	20.60896397113395
40	23.754849523341957	25.941090397224624	29.907008052653882	20.397052026779534
41	22.02925343709667	26.20574060827358	28.87889951538327	22.886106439246475
42	22.389983181287313	26.535978045539014	28.99645790623328	22.077580866940394
43	22.73786396595601	25.93533713176704	29.63622102511684	21.69057787716011
44	22.206837564220823	25.85920225221165	29.393624998321965	22.540335185245556
45	22.801725212535214	26.042156093762888	28.605427630632686	22.550691063069213
46	23.299766225646906	26.86391417662141	28.831914514146323	21.004405083585358
47	21.80219122703728	26.057498134983113	30.838269954721802	21.302040683257808
48	22.20837176834285	25.203329990046853	30.03530587235805	22.552992369252248
49	21.74159016421737	24.696467303233526	31.834543756460437	21.72739877608866
50	21.204235170478842	25.303436809008844	30.805476341613563	22.686851678898748
51	21.367436133959032	25.146756213047254	28.971335313735153	24.514472339258557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	364.0
1	760.5
2	1157.0
3	5088.0
4	9019.0
5	5962.0
6	2905.0
7	2723.0
8	2541.0
9	2574.0
10	2607.0
11	2582.0
12	2557.0
13	2507.0
14	2457.0
15	2311.0
16	2165.0
17	2130.0
18	2095.0
19	2066.5
20	2038.0
21	2068.0
22	2098.0
23	2504.0
24	2910.0
25	3276.5
26	4353.5
27	5064.0
28	5944.5
29	6825.0
30	7684.5
31	8544.0
32	9848.5
33	11153.0
34	13364.5
35	15576.0
36	16264.5
37	16953.0
38	18604.0
39	20255.0
40	21842.0
41	23429.0
42	27073.5
43	30718.0
44	32941.5
45	35165.0
46	40108.0
47	45051.0
48	46425.0
49	47799.0
50	46071.5
51	44344.0
52	39431.5
53	34519.0
54	30951.5
55	27384.0
56	25452.5
57	23521.0
58	22073.5
59	20626.0
60	20136.5
61	19647.0
62	17099.5
63	14552.0
64	12766.0
65	10980.0
66	9091.5
67	7203.0
68	5878.5
69	4554.0
70	4030.5
71	3507.0
72	2638.0
73	1769.0
74	1568.0
75	1281.0
76	1195.0
77	895.0
78	595.0
79	426.5
80	258.0
81	213.5
82	169.0
83	137.5
84	106.0
85	75.5
86	45.0
87	25.0
88	5.0
89	5.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	521443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.213838950770917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63871328375402	22.985469369810925
2	9.673903339539134	6.03919321731399
3	3.782138324507325	3.541651696521306
4	2.090439780969632	2.610026026378837
5	1.3085109967610018	2.0421825759105317
6	0.9192919545184727	1.7216778610247416
7	0.6929054609319164	1.5139767625946972
8	0.5609787200507101	1.4008239537977978
9	0.4466477005034633	1.2547430452122539
>10	6.552546782676013	47.18812704196807
>50	0.29365456928326544	5.617031911657165
>100	0.03407384242738312	1.681416066094917
>500	0.004336670854394216	0.9601185472295872
>1k	0.0012390488155412044	0.4714539814996443
>5k	6.195244077706022E-4	0.9721079429855258
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5027	0.9640555151761553	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1237	0.23722631236779476	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1201	0.23032239381869157	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG	843	0.1616667593581657	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC	820	0.1572559225073498	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	755	0.14479051401591353	No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	688	0.13194155449397152	Illumina Single End Adapter 1 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	676	0.12964024831093715	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	631	0.12101035012455821	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	552	0.1058600844195818	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8355103050573123E-4	0.0	0.0	0.1376948199515575	0.0
2	3.8355103050573123E-4	0.0	0.0	0.7471574074251645	0.0
3	3.8355103050573123E-4	0.0	0.0	1.0010681896199585	0.0
4	3.8355103050573123E-4	0.0	0.0	1.287005482861981	0.0
5	3.8355103050573123E-4	0.0	0.0	2.159967628293025	0.0
6	5.753265457585969E-4	0.0	0.0	2.7136235408280482	0.0
7	5.753265457585969E-4	0.0	0.0	3.3085111891424375	0.0
8	5.753265457585969E-4	0.0	0.0	4.278511745291432	0.0
9	5.753265457585969E-4	0.0	0.0	4.660720347190392	0.0
10	5.753265457585969E-4	0.0	0.0	5.579325065251619	0.0
11	5.753265457585969E-4	0.0	0.0	6.642144970783	0.0
12	5.753265457585969E-4	0.0	0.0	7.666417997748556	0.0
13	5.753265457585969E-4	0.0	0.0	7.996463659498737	0.0
14	7.671020610114625E-4	0.0	0.0	8.138185765270604	0.0
15	7.671020610114625E-4	0.0023013061830343875	0.0	8.385384404431548	0.0
16	7.671020610114625E-4	0.0023013061830343875	0.0	8.845837416553678	0.0
17	7.671020610114625E-4	0.0023013061830343875	0.0	9.42941030946815	0.0
18	7.671020610114625E-4	0.0023013061830343875	0.0	10.060735305680582	0.0
19	7.671020610114625E-4	0.0023013061830343875	0.0	10.484750969904669	0.0
20	0.0013424286067700592	0.0023013061830343875	0.0	10.883644041630628	0.0
21	0.0013424286067700592	0.0023013061830343875	0.0	11.364808809400069	0.0
22	0.0013424286067700592	0.0023013061830343875	0.0	11.864575802149037	0.0
23	0.0013424286067700592	0.0023013061830343875	0.0	12.363192141806486	0.0
24	0.0013424286067700592	0.0023013061830343875	0.0	12.761893438017195	0.0
25	0.0013424286067700592	0.0023013061830343875	0.0	13.125308039421375	0.0
26	0.0013424286067700592	0.0023013061830343875	0.0	13.448066231591948	0.0
27	0.0013424286067700592	0.0023013061830343875	0.0	13.791727954925083	0.0
28	0.0013424286067700592	0.0023013061830343875	0.0	14.133663698620943	0.0
29	0.0013424286067700592	0.0023013061830343875	0.0	14.511269688153835	0.0
30	0.0013424286067700592	0.0023013061830343875	0.0	14.960791495906552	0.0
31	0.001534204122022925	0.0023013061830343875	0.0	15.345876730534306	0.0
32	0.001534204122022925	0.0023013061830343875	0.0	15.696250596901292	0.0
33	0.001534204122022925	0.0023013061830343875	0.0	16.075774341586712	0.0
34	0.001534204122022925	0.0023013061830343875	0.0	16.439572494021398	0.0
35	0.001534204122022925	0.0023013061830343875	0.0	16.839616218838877	0.0
36	0.0017259796372757904	0.0023013061830343875	0.0	17.210893616368423	0.0
37	0.0017259796372757904	0.0023013061830343875	0.0	17.587540728325052	0.0
38	0.0021095306677815216	0.0023013061830343875	0.0	17.993721269630623	0.0
39	0.0021095306677815216	0.0023013061830343875	0.0	18.358478299641572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGT	30	2.1632113E-6	45.000004	4
CGAACAC	20	7.0297363E-4	45.0	34
CGTTCTA	20	7.0297363E-4	45.0	18
TCGTGCG	25	3.8878643E-5	45.0	1
TTGACGA	25	3.8878643E-5	45.0	2
GCGATAT	40	6.8030204E-9	45.0	9
CATCGTT	25	3.8878643E-5	45.0	36
TAGATCG	20	7.0297363E-4	45.0	1
ACCCGTA	20	7.0297363E-4	45.0	15
ACGTAAG	20	7.0297363E-4	45.0	1
TACCGGC	20	7.0297363E-4	45.0	12
TCTATAC	25	3.8878643E-5	45.0	14
TCAAGCG	95	0.0	42.63158	17
CGTTATT	370	0.0	42.56757	1
CGTTTTT	2310	0.0	42.272728	1
CGGTAGT	80	0.0	42.1875	12
CGTTAGG	70	0.0	41.785713	2
GCGAGAC	105	0.0	40.714287	21
ACACGAC	100	0.0	40.5	26
TTGCACG	50	1.0786607E-9	40.5	1
>>END_MODULE