##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935974.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	737671
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00424986206588	33.0	31.0	34.0	30.0	34.0
2	32.14152650707429	33.0	31.0	34.0	30.0	34.0
3	32.01422043160162	33.0	31.0	34.0	30.0	34.0
4	35.711132740747566	37.0	35.0	37.0	33.0	37.0
5	32.000115227520126	37.0	35.0	37.0	0.0	37.0
6	33.786662346764345	37.0	35.0	37.0	17.0	37.0
7	35.40814672123481	37.0	35.0	37.0	32.0	37.0
8	35.614988253571035	37.0	35.0	37.0	33.0	37.0
9	37.582386999082246	39.0	37.0	39.0	35.0	39.0
10	37.20381037074794	39.0	37.0	39.0	34.0	39.0
11	37.19858581942356	39.0	37.0	39.0	34.0	39.0
12	37.20415876454408	39.0	37.0	39.0	34.0	39.0
13	37.140820230156805	39.0	37.0	39.0	33.0	39.0
14	38.449179918961164	40.0	38.0	41.0	34.0	41.0
15	38.51940905905207	40.0	38.0	41.0	34.0	41.0
16	38.52180443585284	40.0	38.0	41.0	34.0	41.0
17	38.4214710894152	40.0	38.0	41.0	34.0	41.0
18	38.22794443593418	40.0	38.0	41.0	34.0	41.0
19	37.99909444725358	40.0	37.0	41.0	34.0	41.0
20	37.76661411388004	40.0	35.0	41.0	33.0	41.0
21	37.820830695526865	40.0	35.0	41.0	33.0	41.0
22	37.837361100002575	40.0	35.0	41.0	33.0	41.0
23	37.780025241604996	40.0	35.0	41.0	33.0	41.0
24	37.78370981101331	40.0	35.0	41.0	33.0	41.0
25	37.61189066670643	40.0	35.0	41.0	33.0	41.0
26	37.57160712566984	40.0	35.0	41.0	33.0	41.0
27	37.49764325830892	40.0	35.0	41.0	33.0	41.0
28	37.410741373864504	40.0	35.0	41.0	33.0	41.0
29	37.36211536037068	40.0	35.0	41.0	33.0	41.0
30	37.15452823819833	40.0	35.0	41.0	32.0	41.0
31	37.00488293561764	40.0	35.0	41.0	32.0	41.0
32	36.70128282120349	40.0	35.0	41.0	31.0	41.0
33	36.382962052188574	40.0	35.0	41.0	30.0	41.0
34	36.00675504391524	40.0	35.0	41.0	27.0	41.0
35	35.78534875303489	40.0	35.0	41.0	25.0	41.0
36	35.631227200201714	40.0	35.0	41.0	24.0	41.0
37	35.48893476902305	39.0	35.0	41.0	23.0	41.0
38	35.38942834949456	39.0	35.0	41.0	23.0	41.0
39	35.259756720814565	39.0	35.0	41.0	22.0	41.0
40	35.20498975830689	39.0	35.0	41.0	22.0	41.0
41	35.07286175002135	39.0	35.0	41.0	21.0	41.0
42	35.04295546388566	39.0	35.0	41.0	21.0	41.0
43	34.977088702145	39.0	35.0	41.0	20.0	41.0
44	35.001760947631126	39.0	35.0	41.0	20.0	41.0
45	35.00697600963031	39.0	35.0	41.0	20.0	41.0
46	34.87060898422197	39.0	35.0	41.0	20.0	41.0
47	34.71584080165819	38.0	35.0	41.0	20.0	41.0
48	34.71328952880078	38.0	35.0	41.0	20.0	41.0
49	34.70314001770437	38.0	35.0	41.0	20.0	41.0
50	34.53866425547432	38.0	34.0	41.0	19.0	41.0
51	33.70826560892322	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	33.0
10	13.0
11	25.0
12	17.0
13	17.0
14	21.0
15	42.0
16	68.0
17	135.0
18	323.0
19	705.0
20	1504.0
21	2839.0
22	4227.0
23	5865.0
24	8136.0
25	11343.0
26	13961.0
27	14673.0
28	13019.0
29	12202.0
30	12404.0
31	14969.0
32	18112.0
33	24955.0
34	38545.0
35	51387.0
36	52644.0
37	77647.0
38	128037.0
39	229573.0
40	219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48361261321104	16.813593051644972	20.585328689890208	19.117465645253777
2	35.990841445576685	27.062742062518385	21.735163778974638	15.211252712930289
3	27.88844891557347	27.08904104946514	30.240174820482302	14.782335214479083
4	23.59547820098662	22.734389721162955	36.687764599665705	16.98236747818472
5	23.93275593048934	33.82985097692603	26.51751254963256	15.719880542952074
6	22.237420204942314	34.96626544896031	29.82535574802317	12.970958598074208
7	70.78657016474824	5.130878128596623	20.148955293077808	3.9335964135773263
8	71.53826028134493	9.087926731564613	15.304519223339403	4.069293763751049
9	66.3039485082103	6.606603756959403	16.734153843651168	10.35529389117913
10	36.53674876740444	23.672612858577878	25.13342669021827	14.657211683799417
11	27.874350489581403	24.143825635005307	30.673023610796683	17.30880026461661
12	22.9087221810265	20.798296259443575	35.12405937064084	21.168922188889088
13	23.398235798885953	22.485091592322323	37.14311664685205	16.973555961939674
14	18.564644672218375	28.142627268795984	34.23463847704464	19.058089581941
15	17.545897832502565	24.313955679428904	38.8410280463784	19.29911844169013
16	20.23557927585604	23.649025107398828	34.354475097977286	21.76092051876785
17	20.45125808117711	25.37120206704615	33.68479986335372	20.492739988423022
18	20.955412372182177	23.93424711016158	33.87716204107251	21.233178476583735
19	21.162279661258204	25.60314828697346	31.88047245994488	21.354099591823456
20	23.6797976333623	24.79723345502263	33.68642660481434	17.83654230680073
21	21.56069575732271	28.011674581215747	32.70943279592122	17.718196865540328
22	20.281534722118668	22.99304161340218	34.881132645854315	21.844291018624833
23	20.878142152802535	26.110556060899775	33.41923431990684	19.592067466390844
24	20.423739038134887	25.38597830198015	33.45136246375417	20.738920196130795
25	18.66414702489321	29.728429069327657	31.48869889151126	20.118725014267877
26	19.37205068384144	24.802927050134816	34.42998301410792	21.395039251915826
27	21.82287225605995	25.532791718801473	32.15010485704332	20.49423116809526
28	18.44385911876704	26.637755855930358	34.28154285582597	20.636842169476637
29	20.108557880138978	23.525257194603014	34.228402634778924	22.137782290479088
30	19.940596824329546	27.116153407142207	33.747971656741285	19.195278111786962
31	20.649178292219702	26.419636938418346	30.399053236469918	22.532131532892034
32	24.29497702905496	26.583937825941373	30.07126483215417	19.049820312849494
33	23.585988875799647	25.44874341000256	30.28287678382368	20.68239093037411
34	20.822968504929705	27.654198145243612	29.39508263168811	22.127750718138575
35	22.276868685362444	27.35704670510295	30.030596295638574	20.335488313896033
36	20.269740846529142	29.558163463115672	29.92784046004249	20.2442552303127
37	20.210771468581523	28.13801816799088	31.852817855114274	19.798392508313327
38	21.543343848409386	27.985646717845757	28.81623379528272	21.65477563846213
39	22.959557851671004	27.679819323248438	29.330961905781844	20.02966091929871
40	22.01740342239291	27.012448639027426	30.195168306738367	20.774979631841294
41	19.283664397814203	27.75952965481902	30.622052378363797	22.33475356900298
42	20.337115055356655	27.39866417413725	31.14410082543573	21.120119945070364
43	21.398292734837074	26.000073203365726	30.249664145669275	22.351969916127924
44	20.703131883997067	26.02352539275639	30.47022317537222	22.803119547874324
45	20.777690867609003	26.043181852072266	29.1261280435316	24.05299923678713
46	23.272027773899204	26.888274040866456	28.989210637262413	20.850487547971927
47	19.373677425302066	27.085109757601966	32.56939746851916	20.971815348576804
48	20.10679557688997	26.682897931462673	30.63425293931848	22.576053552328883
49	20.469152237243975	24.504555553898687	33.01946260595848	22.006829602898854
50	20.45478268767513	25.576849300026705	31.671300620466305	22.29706739183186
51	19.748370208399137	24.819465588317826	30.199099598601542	25.233064604681495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	400.0
1	730.0
2	1060.0
3	10288.5
4	19517.0
5	12975.5
6	6434.0
7	6298.0
8	6162.0
9	6090.0
10	6018.0
11	5928.5
12	5839.0
13	5720.5
14	5602.0
15	5298.0
16	4994.0
17	4885.0
18	4776.0
19	4698.0
20	4620.0
21	4507.5
22	4395.0
23	4672.5
24	4950.0
25	5236.5
26	6204.5
27	6886.0
28	8484.0
29	10082.0
30	11425.0
31	12768.0
32	14447.0
33	16126.0
34	18423.0
35	20720.0
36	22730.5
37	24741.0
38	26001.0
39	27261.0
40	30792.5
41	34324.0
42	38411.0
43	42498.0
44	61617.5
45	80737.0
46	69735.0
47	58733.0
48	58624.5
49	58516.0
50	53901.0
51	49286.0
52	45957.5
53	42629.0
54	39559.0
55	36489.0
56	34047.0
57	31605.0
58	28912.0
59	26219.0
60	24680.5
61	23142.0
62	20510.5
63	17879.0
64	15128.0
65	12377.0
66	10031.5
67	7686.0
68	6702.0
69	5718.0
70	4749.0
71	3780.0
72	3369.5
73	2959.0
74	2405.0
75	1493.5
76	1136.0
77	878.0
78	620.0
79	481.0
80	342.0
81	268.5
82	195.0
83	120.5
84	46.0
85	30.0
86	14.0
87	11.0
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	2.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	737671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.890688887371994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06643041552432	27.300765888165134
2	8.460269565399708	6.072898057501255
3	3.508939547841016	3.77814772908473
4	1.9365545604085637	2.7801710896418075
5	1.2852919014510236	2.3065005882218736
6	0.9821204494625844	2.1149387700952538
7	0.7507032711225274	1.8860280285413769
8	0.6389250387298626	1.8345167829924478
9	0.5190007369043733	1.6764564582496497
>10	5.747366930187787	40.501411837842696
>50	0.07550526320548687	1.7006230431278493
>100	0.022343393949886645	1.4507835295342746
>500	0.0026966165111932156	0.7061042109341028
>1k	0.0023113855810227564	1.492123877893252
>5k	0.0011556927905113782	2.8840077806685827
>10k+	3.8523093017045934E-4	1.5145223275058082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10954	1.4849438299729825	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	9352	1.2677738449796725	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	6127	0.8305870774369604	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	5380	0.7293224215131136	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	2671	0.3620855367772354	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	2179	0.29538913689164953	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT	1932	0.26190537516047124	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT	1575	0.21350981670690594	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1220	0.16538538183011126	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTC	1215	0.16470757288818458	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAGGAATT	914	0.1239034745841981	No Hit
GAATATGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	824	0.11170291362951777	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTA	797	0.10804274534311367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.422471535413484E-4	0.0	0.0	0.08215044376151429	0.0
2	5.422471535413484E-4	0.0	0.0	0.5113390657894915	0.0
3	5.422471535413484E-4	0.0	0.0	0.697329839454174	0.0
4	5.422471535413484E-4	0.0	0.0	1.247439576721872	0.0
5	5.422471535413484E-4	0.0	0.0	3.0092005785777127	0.0
6	5.422471535413484E-4	0.0	0.0	3.8964524835597443	0.0
7	5.422471535413484E-4	0.0	0.0	4.574939234428356	0.0
8	5.422471535413484E-4	0.0	0.0	5.4799497336888665	0.0
9	5.422471535413484E-4	0.0	0.0	5.856947067188489	0.0
10	5.422471535413484E-4	0.0	0.0	7.4394953847989145	0.0
11	5.422471535413484E-4	0.0	0.0	8.221280218417153	0.0
12	5.422471535413484E-4	0.0	0.0	9.687380959804575	0.0
13	5.422471535413484E-4	0.0	0.0	10.060582563229406	0.0
14	5.422471535413484E-4	0.0	0.0	10.261214010039707	0.0
15	5.422471535413484E-4	0.0	0.0	10.576395168035615	0.0
16	5.422471535413484E-4	0.0	0.0	10.961797332415129	0.0
17	5.422471535413484E-4	0.0	0.0	11.395595055248206	0.0
18	6.778089419266854E-4	0.0	0.0	11.869112382078189	0.0
19	6.778089419266854E-4	0.0	0.0	12.322295440650372	0.0
20	6.778089419266854E-4	0.0	0.0	12.638154407588207	0.0
21	8.133707303120226E-4	0.0	0.0	12.981803541145036	0.0
22	8.133707303120226E-4	0.0	0.0	13.410449916019472	0.0
23	8.133707303120226E-4	0.0	0.0	13.806968147046582	0.0
24	8.133707303120226E-4	0.0	0.0	14.124860540810198	0.0
25	8.133707303120226E-4	0.0	0.0	14.407506869593627	0.0
26	8.133707303120226E-4	0.0	0.0	14.706962860136835	0.0
27	8.133707303120226E-4	0.0	0.0	15.007503344987128	0.0
28	8.133707303120226E-4	0.0	0.0	15.302621358302007	0.0
29	8.133707303120226E-4	0.0	0.0	15.602484034210374	0.0
30	8.133707303120226E-4	0.0	0.0	15.980294738440307	0.0
31	8.133707303120226E-4	0.0	0.0	16.30604971593027	0.0
32	8.133707303120226E-4	0.0	0.0	16.62272205359842	0.0
33	8.133707303120226E-4	0.0	0.0	16.941292256303964	0.0
34	9.489325186973597E-4	0.0	0.0	17.246984089112896	0.0
35	9.489325186973597E-4	0.0	0.0	17.56460535929974	0.0
36	9.489325186973597E-4	0.0	0.0	17.871788371780916	0.0
37	9.489325186973597E-4	0.0	0.0	18.18615615904651	0.0
38	9.489325186973597E-4	0.0	0.0	18.496999339814092	0.0
39	9.489325186973597E-4	0.0	0.0	18.830888024607177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	25	3.8894417E-5	45.000004	1
TTACGCG	25	3.8894417E-5	45.000004	1
CGACGGT	20	7.031642E-4	45.0	28
TTGCTCG	20	7.031642E-4	45.0	1
CCAATCG	40	6.8084773E-9	45.0	40
AATAGCG	20	7.031642E-4	45.0	1
GTACGTC	20	7.031642E-4	45.0	43
CGTTATT	750	0.0	43.5	1
CGTTTTT	5730	0.0	42.997383	1
CGGCGAA	190	0.0	42.63158	31
CGATGAA	1355	0.0	42.509228	19
CACAACG	75	0.0	42.0	12
TAATACG	270	0.0	41.666668	4
CCGATGA	1375	0.0	41.072727	18
CGACAGG	110	0.0	40.909092	2
CGATGCG	55	6.002665E-11	40.909092	10
CGAACCC	50	1.0804797E-9	40.500004	34
TATTGCG	50	1.0804797E-9	40.500004	1
CAATCGA	45	1.9266736E-8	40.0	41
GATGAAT	1525	0.0	39.540985	20
>>END_MODULE