##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935972.fastq
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	403979
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18384123927234	33.0	31.0	34.0	30.0	34.0
2	32.30813482879061	34.0	31.0	34.0	30.0	34.0
3	32.21999410860465	34.0	31.0	34.0	30.0	34.0
4	35.85114820324819	37.0	35.0	37.0	35.0	37.0
5	32.103955898697706	37.0	35.0	37.0	0.0	37.0
6	33.928466578708296	37.0	35.0	37.0	17.0	37.0
7	35.59666962886685	37.0	35.0	37.0	32.0	37.0
8	35.834290891358215	37.0	35.0	37.0	35.0	37.0
9	37.80577950834078	39.0	38.0	39.0	35.0	39.0
10	37.46312060775436	39.0	37.0	39.0	35.0	39.0
11	37.39482250314992	39.0	37.0	39.0	35.0	39.0
12	37.268135224850795	39.0	37.0	39.0	35.0	39.0
13	37.171632683877135	39.0	37.0	39.0	34.0	39.0
14	38.41371952502482	40.0	38.0	41.0	34.0	41.0
15	38.57103463298835	40.0	38.0	41.0	35.0	41.0
16	38.51440297639234	40.0	38.0	41.0	34.0	41.0
17	38.51748481975548	40.0	38.0	41.0	34.0	41.0
18	38.41499434376539	40.0	37.0	41.0	34.0	41.0
19	38.233170536092224	40.0	37.0	41.0	34.0	41.0
20	38.23033375497241	40.0	36.0	41.0	34.0	41.0
21	38.130927597721666	40.0	36.0	41.0	34.0	41.0
22	38.19096289658621	40.0	36.0	41.0	34.0	41.0
23	38.2017604875501	40.0	36.0	41.0	34.0	41.0
24	38.14526002589243	40.0	36.0	41.0	34.0	41.0
25	37.891132954930825	40.0	35.0	41.0	34.0	41.0
26	37.944828815359216	40.0	35.0	41.0	34.0	41.0
27	37.94405402261009	40.0	35.0	41.0	34.0	41.0
28	37.85916594674476	40.0	35.0	41.0	34.0	41.0
29	37.83288735305548	40.0	35.0	41.0	34.0	41.0
30	37.64737275947512	40.0	35.0	41.0	33.0	41.0
31	37.57306939222088	40.0	35.0	41.0	33.0	41.0
32	37.41652412625409	40.0	35.0	41.0	33.0	41.0
33	37.26700892868194	40.0	35.0	41.0	33.0	41.0
34	37.09989380636122	40.0	35.0	41.0	33.0	41.0
35	37.00718354171875	40.0	35.0	41.0	32.0	41.0
36	36.86311169640006	40.0	35.0	41.0	32.0	41.0
37	36.78367687429297	40.0	35.0	41.0	31.0	41.0
38	36.65139029504009	39.0	35.0	41.0	31.0	41.0
39	36.57185893326138	39.0	35.0	41.0	31.0	41.0
40	36.499780929206715	39.0	35.0	41.0	31.0	41.0
41	36.40681570081613	39.0	35.0	41.0	31.0	41.0
42	36.38199015295349	39.0	35.0	41.0	31.0	41.0
43	36.27957146287307	39.0	35.0	41.0	30.0	41.0
44	36.18867812435795	39.0	35.0	41.0	30.0	41.0
45	36.17230103544986	39.0	35.0	41.0	30.0	41.0
46	36.05066352458915	39.0	35.0	41.0	30.0	41.0
47	35.995965136801665	39.0	35.0	41.0	30.0	41.0
48	35.94429908485342	38.0	35.0	41.0	30.0	41.0
49	35.88437517791766	38.0	35.0	41.0	30.0	41.0
50	35.77212924434191	38.0	35.0	41.0	29.0	41.0
51	34.81209914376738	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	10.0
9	12.0
10	13.0
11	17.0
12	13.0
13	12.0
14	19.0
15	25.0
16	32.0
17	60.0
18	108.0
19	197.0
20	335.0
21	523.0
22	818.0
23	1237.0
24	1936.0
25	2970.0
26	4383.0
27	5015.0
28	5139.0
29	5169.0
30	5827.0
31	7051.0
32	8928.0
33	14082.0
34	25308.0
35	35949.0
36	28918.0
37	44710.0
38	74144.0
39	130903.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50908834370103	18.674733092561745	23.81014854732548	16.006030016411742
2	34.83695934689675	24.063874607343454	24.397802856089054	16.70136318967075
3	31.611296626804858	23.302201352050478	28.668569405835452	16.417932615309212
4	28.490342319774047	24.533948546830405	28.755950185529443	18.2197589478661
5	21.854848890660158	37.073709276967364	23.922530626591975	17.148911205780497
6	24.476024743860446	33.889880414576005	27.76901769646442	13.865077145099129
7	77.58720131491984	6.420630775362085	11.65926941746972	4.3328984922483595
8	78.04836390010372	6.302307793226876	11.304795546303149	4.344532760366256
9	72.26588510788928	8.324690144784753	13.313563328786893	6.095861418539082
10	40.50086761935645	25.205023033375497	20.531017701415173	13.76309164585288
11	31.345936298668992	23.879706618413334	27.108587327559107	17.66576975535857
12	27.892786506229285	21.42586619601514	31.52713383616475	19.154213461590825
13	24.34606749360734	25.05402508546236	32.05513157862166	18.544775842308635
14	19.428980219268823	27.888330829077752	32.31430346626929	20.36838548538414
15	19.60621715485211	24.23096250052602	36.810329249787735	19.352491094834136
16	21.34962460919998	23.137836372682738	34.6292752841113	20.883263734005975
17	20.938464623161103	23.479685825253295	31.693974191727786	23.887875359857812
18	22.864802378341448	24.259676864391466	32.03136796714681	20.84415279012028
19	23.724748068587722	24.8386673564715	29.99809396032962	21.438490614611155
20	24.799308874966272	24.981744100559684	30.559756819042573	19.65919020543147
21	23.4631008047448	25.46444245864265	30.092405793370446	20.980050943242098
22	22.11451585354684	23.907678369420193	30.36197426103832	23.615831515994643
23	21.868463459734294	25.6396990932697	29.990915369363258	22.500922077632747
24	21.33328712631102	24.02723903965305	32.981664888521436	21.657808945514496
25	21.43403493745962	25.351565304137097	30.3567759710282	22.857623787375086
26	19.354966471029435	27.01625579547452	31.279348679015495	22.349429054480556
27	21.426361271254198	25.559991979781127	31.370690060621964	21.64295668834271
28	19.58913705910456	25.720396357236393	33.39876577742903	21.291700806230025
29	20.58918904200466	24.342106891694865	32.8148245329584	22.25387953334208
30	21.43601523841586	25.898623443297797	31.706598610323805	20.958762707962542
31	24.227001898613544	25.003774948697828	28.877986231957603	21.89123692073103
32	23.38265107839764	26.354092663232493	29.711197859294668	20.5520583990752
33	23.567561680186348	26.22859109013092	29.1661200210902	21.03772720859253
34	20.560474678139208	26.116456548483956	30.43400770832147	22.889061065055362
35	21.756081380467794	25.04857925783271	30.714715368868184	22.480623992831312
36	22.79747214582936	27.51504409882692	28.575990336131333	21.111493419212383
37	22.42418541557853	27.12393465996995	29.536683837526205	20.91519608692531
38	21.608796496847607	28.229190131170185	28.49851106121853	21.663502310763675
39	22.346458603046198	25.969419202483294	30.003539787959276	21.680582406511228
40	22.591025771141567	25.710494852455202	29.856749979578147	21.841729396825084
41	20.388683570185577	25.707771938640374	29.622084316263965	24.281460174910084
42	21.865493008299936	25.863720638944105	28.906700595823047	23.36408575693291
43	23.321014211134738	24.743117835333024	29.879028365335824	22.056839588196418
44	21.86326516972417	26.499644783515976	29.116117421945198	22.520972624814657
45	21.719940888016456	26.920458736716512	27.94031372917899	23.41928664608804
46	21.789251421484778	27.135073852848784	28.771545055559816	22.304129670106615
47	21.394428918334864	25.640936781367348	30.98106584748217	21.983568452815618
48	22.09793083303835	24.825795400255952	30.20033219548541	22.875941571220288
49	21.466957440857072	24.447310379995	31.214741360318232	22.870990818829693
50	20.444379534579767	25.13001913465799	31.058792660014507	23.366808670747737
51	20.808259835288467	25.05575784879907	28.776743345569944	25.359238970342517
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	391.0
1	737.5
2	1084.0
3	4168.0
4	7252.0
5	4726.0
6	2200.0
7	2125.0
8	2050.0
9	2045.5
10	2041.0
11	1986.0
12	1931.0
13	1894.5
14	1858.0
15	1725.0
16	1592.0
17	1629.5
18	1667.0
19	1734.5
20	1802.0
21	1763.5
22	1725.0
23	1988.0
24	2251.0
25	2553.5
26	3552.5
27	4249.0
28	5072.5
29	5896.0
30	6308.5
31	6721.0
32	7637.5
33	8554.0
34	9997.0
35	11440.0
36	11607.5
37	11775.0
38	12935.5
39	14096.0
40	16592.5
41	19089.0
42	21957.5
43	24826.0
44	28394.5
45	31963.0
46	33429.0
47	34895.0
48	34505.0
49	34115.0
50	30053.0
51	25991.0
52	24537.5
53	23084.0
54	21405.5
55	19727.0
56	18603.5
57	17480.0
58	17177.5
59	16875.0
60	16367.0
61	15859.0
62	15124.0
63	14389.0
64	12380.0
65	10371.0
66	8637.0
67	6903.0
68	5904.0
69	4905.0
70	4264.0
71	3623.0
72	3037.5
73	2452.0
74	2033.0
75	1444.5
76	1275.0
77	909.5
78	544.0
79	458.0
80	372.0
81	243.5
82	115.0
83	86.5
84	58.0
85	36.5
86	15.0
87	9.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	403979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.23285359903662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.84112394494883	23.06269013760904
2	9.415527919419214	5.881476101297244
3	3.6906809454507292	3.4581149295005007
4	2.0291358868751477	2.535028163492913
5	1.3449419346358191	2.1003187271842814
6	0.9616913822122852	1.8021819688854883
7	0.710323671738322	1.552980467313321
8	0.5827293534103561	1.4560240466341539
9	0.4790891079960215	1.3466987973839506
>10	6.638650953904085	48.13154276869861
>50	0.2718275278607726	5.164677711946559
>100	0.031088778846246716	2.054517955986454
>500	0.0023914445266343632	0.4966993846574134
>1k	7.971481755447877E-4	0.9570488394100738
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3844	0.9515346094722746	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	753	0.1863958275058852	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	664	0.16436497936773942	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	578	0.1430767440881828	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	467	0.115600068320383	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	423	0.10470841306107496	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	419	0.10371826258295605	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10124288638765877	0.0
2	0.0	0.0	0.0	0.5277502048373801	0.0
3	0.0	0.0	0.0	0.7057297532792546	0.0
4	0.0	0.0	0.0	0.9475740075597989	0.0
5	0.0	0.0	0.0	1.6899393285294533	0.0
6	0.0	0.0	0.0	2.261008616784536	0.0
7	0.0	0.0	0.0	2.8102945945210025	0.0
8	0.0	0.0	0.0	3.688062993373418	0.0
9	0.0	0.0	0.0	4.083380571762394	0.0
10	0.0	0.0	0.0	4.962386658712457	0.0
11	0.0	0.0	0.0	5.971845071154688	0.0
12	0.0	0.0	0.0	6.846395480953218	0.0
13	0.0	0.0	0.0	7.170917300156692	0.0
14	0.0	0.0	0.0	7.3048351523222745	0.0
15	0.0	0.0	0.0	7.53628282658257	0.0
16	0.0	0.0	0.0	8.037051430891209	0.0
17	0.0	0.0	0.0	8.62767619108914	0.0
18	0.0	0.0	0.0	9.2918196242874	0.0
19	0.0	0.0	0.0	9.694315793642739	0.0
20	0.0	0.0	0.0	10.071315588186515	0.0
21	0.0	0.0	0.0	10.538666613858641	0.0
22	2.47537619529728E-4	0.0	0.0	11.034484465776686	0.0
23	4.95075239059456E-4	0.0	0.0	11.512727146708121	0.0
24	4.95075239059456E-4	0.0	0.0	11.906064424140858	0.0
25	4.95075239059456E-4	0.0	0.0	12.242220511462229	0.0
26	4.95075239059456E-4	0.0	0.0	12.546444245864265	0.0
27	4.95075239059456E-4	0.0	0.0	12.864530086959967	0.0
28	4.95075239059456E-4	0.0	0.0	13.214053205735942	0.0
29	4.95075239059456E-4	0.0	0.0	13.555407583067437	0.0
30	4.95075239059456E-4	0.0	0.0	13.98934103010305	0.0
31	4.95075239059456E-4	0.0	0.0	14.357924545582815	0.0
32	4.95075239059456E-4	0.0	0.0	14.73566695298518	0.0
33	4.95075239059456E-4	0.0	0.0	15.097071877498584	0.0
34	4.95075239059456E-4	0.0	0.0	15.44510977055738	0.0
35	4.95075239059456E-4	0.0	0.0	15.833743833219053	0.0
36	4.95075239059456E-4	0.0	0.0	16.169404845301365	0.0
37	4.95075239059456E-4	0.0	0.0	16.51843288883828	0.0
38	4.95075239059456E-4	0.0	0.0	16.861272491886954	0.0
39	4.95075239059456E-4	0.0	0.0	17.231836308322958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGG	65	0.0	45.000004	2
TCCGGAT	20	7.027849E-4	45.0	14
TAGCGAG	20	7.027849E-4	45.0	1
GCACGTC	20	7.027849E-4	45.0	38
CAACCGG	20	7.027849E-4	45.0	2
TTCGTTC	20	7.027849E-4	45.0	30
TAACGAC	20	7.027849E-4	45.0	21
GCGTTCG	25	3.886298E-5	45.0	10
TAGCCGT	20	7.027849E-4	45.0	44
ATCCGTC	20	7.027849E-4	45.0	28
TTATACG	25	3.886298E-5	45.0	1
TAACCGA	20	7.027849E-4	45.0	37
TCTACGG	20	7.027849E-4	45.0	2
AACGATC	20	7.027849E-4	45.0	33
GAACGAT	20	7.027849E-4	45.0	32
TCCGTCA	25	3.886298E-5	45.0	29
ATAACGG	45	3.8380676E-10	45.0	2
ATAACCG	20	7.027849E-4	45.0	36
CACGTCA	20	7.027849E-4	45.0	39
ATACGAG	20	7.027849E-4	45.0	1
>>END_MODULE