##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935971.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	508125
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12099188191882	33.0	31.0	34.0	30.0	34.0
2	32.24784452644526	34.0	31.0	34.0	30.0	34.0
3	32.17432127921279	33.0	31.0	34.0	30.0	34.0
4	35.81382730627306	37.0	35.0	37.0	35.0	37.0
5	32.17088511685117	37.0	35.0	37.0	0.0	37.0
6	33.9303655596556	37.0	35.0	37.0	17.0	37.0
7	35.55666617466175	37.0	35.0	37.0	32.0	37.0
8	35.763642804428045	37.0	35.0	37.0	35.0	37.0
9	37.70720590405904	39.0	37.0	39.0	35.0	39.0
10	37.367852398523986	39.0	37.0	39.0	34.0	39.0
11	37.31387749077491	39.0	37.0	39.0	34.0	39.0
12	37.16191488314883	39.0	37.0	39.0	34.0	39.0
13	37.04610086100861	39.0	37.0	39.0	33.0	39.0
14	38.25373874538745	40.0	37.0	41.0	33.0	41.0
15	38.42292349323493	40.0	38.0	41.0	34.0	41.0
16	38.37990651906519	40.0	37.0	41.0	34.0	41.0
17	38.360724231242315	40.0	37.0	41.0	34.0	41.0
18	38.24703370233702	40.0	37.0	41.0	34.0	41.0
19	38.07453087330873	40.0	37.0	41.0	34.0	41.0
20	38.059371217712176	40.0	36.0	41.0	34.0	41.0
21	37.98818597785978	40.0	36.0	41.0	34.0	41.0
22	38.035221648216485	40.0	36.0	41.0	34.0	41.0
23	38.03401328413284	40.0	35.0	41.0	34.0	41.0
24	37.988363099630995	40.0	35.0	41.0	34.0	41.0
25	37.72384551045511	40.0	35.0	41.0	33.0	41.0
26	37.74795768757688	40.0	35.0	41.0	33.0	41.0
27	37.73841082410824	40.0	35.0	41.0	34.0	41.0
28	37.657523247232476	40.0	35.0	41.0	33.0	41.0
29	37.62089446494465	40.0	35.0	41.0	33.0	41.0
30	37.426741451414514	40.0	35.0	41.0	33.0	41.0
31	37.34778450184502	40.0	35.0	41.0	33.0	41.0
32	37.20479212792128	40.0	35.0	41.0	33.0	41.0
33	37.04030307503075	40.0	35.0	41.0	32.0	41.0
34	36.86250430504305	40.0	35.0	41.0	32.0	41.0
35	36.765449446494465	40.0	35.0	41.0	31.0	41.0
36	36.61501402214022	39.0	35.0	41.0	31.0	41.0
37	36.50418105781058	39.0	35.0	41.0	31.0	41.0
38	36.38530086100861	39.0	35.0	41.0	30.0	41.0
39	36.271954735547354	39.0	35.0	41.0	30.0	41.0
40	36.191429274292744	39.0	35.0	41.0	30.0	41.0
41	36.09518917589176	39.0	35.0	41.0	30.0	41.0
42	36.04804723247233	39.0	35.0	41.0	30.0	41.0
43	35.95987798277983	39.0	35.0	41.0	30.0	41.0
44	35.88356211562115	39.0	35.0	41.0	29.0	41.0
45	35.87790012300123	38.0	35.0	41.0	29.0	41.0
46	35.77601574415744	38.0	35.0	41.0	29.0	41.0
47	35.68738794587946	38.0	35.0	41.0	29.0	41.0
48	35.65843640836408	38.0	35.0	41.0	29.0	41.0
49	35.60449889298893	38.0	35.0	40.0	29.0	41.0
50	35.47384403444035	38.0	35.0	40.0	28.0	41.0
51	34.5119704797048	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	16.0
9	22.0
10	20.0
11	17.0
12	16.0
13	9.0
14	18.0
15	24.0
16	54.0
17	79.0
18	147.0
19	289.0
20	569.0
21	1011.0
22	1499.0
23	2056.0
24	2949.0
25	4330.0
26	5552.0
27	6408.0
28	6793.0
29	7133.0
30	7853.0
31	9726.0
32	12352.0
33	18683.0
34	33255.0
35	45624.0
36	38658.0
37	56819.0
38	91145.0
39	154881.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24526445264453	18.94277982779828	22.68929889298893	15.122656826568265
2	34.0530381303813	24.065731857318575	24.415448954489545	17.465781057810577
3	31.17579335793358	23.973628536285364	28.323542435424354	16.527035670356703
4	28.13579335793358	24.675227552275523	28.810627306273062	18.378351783517836
5	23.503468634686346	35.74848708487085	23.357244772447725	17.39079950799508
6	25.97687576875769	34.04792127921279	25.834194341943416	14.1410086100861
7	76.369987699877	6.303763837638376	12.454022140221403	4.872226322263223
8	77.23768757687577	7.069126691266912	11.493628536285364	4.1995571955719555
9	71.49638376383763	8.041525215252152	13.205412054120542	7.256678966789668
10	41.03950799507995	24.741549815498153	20.29421894218942	13.924723247232473
11	33.67143911439114	23.799655596555965	25.2689790897909	17.259926199261994
12	29.179237392373924	21.4640098400984	29.473849938499384	19.88290282902829
13	25.855055350553506	23.31552275522755	31.39719557195572	19.432226322263222
14	21.61908979089791	27.18071340713407	30.68221402214022	20.517982779827797
15	20.005707257072572	23.50209102091021	35.865584255842556	20.62661746617466
16	22.55271832718327	22.83788437884379	33.252250922509226	21.357146371463713
17	22.51690036900369	22.88610086100861	30.570824108241084	24.02617466174662
18	23.742386223862237	23.430061500615007	31.560344403444034	21.26720787207872
19	25.14538745387454	24.822041820418203	28.403837638376384	21.628733087330872
20	25.89953259532595	24.7370233702337	29.622829028290283	19.740615006150062
21	24.217859778597788	26.119753997539974	29.4039852398524	20.25840098400984
22	22.33938499384994	23.59773677736777	30.30769987699877	23.755178351783517
23	22.654071340713408	25.387847478474786	29.342779827798278	22.615301353013532
24	22.871143911439116	24.128314883148832	30.917982779827796	22.082558425584256
25	21.97963099630996	25.43783517835178	29.81648216482165	22.766051660516606
26	20.366445264452647	26.532054120541204	30.05677736777368	23.044723247232472
27	21.95719557195572	25.69643296432964	29.990061500615006	22.35630996309963
28	20.20428044280443	25.12570725707257	32.60634686346863	22.063665436654368
29	21.510258302583026	23.73648216482165	31.20610086100861	23.547158671586715
30	22.088659286592865	25.375252152521526	31.094317343173433	21.441771217712176
31	23.66878228782288	24.777170971709715	29.009790897908978	22.544255842558425
32	25.635227552275524	25.382337023370233	27.530036900369005	21.452398523985238
33	25.395325953259533	25.494907749077488	27.268487084870845	21.841279212792127
34	22.649348093480935	26.160885608856088	28.843296432964326	22.346469864698648
35	24.329249692496923	24.821648216482163	28.19030750307503	22.65879458794588
36	24.660861008610084	27.430651906519067	27.127773677736776	20.780713407134073
37	23.12088560885609	27.61210332103321	28.97849938499385	20.288511685116852
38	23.80319803198032	26.924280442804427	27.044132841328413	22.228388683886838
39	24.24088560885609	25.967232472324724	27.222829028290285	22.569052890528905
40	24.021057810578107	25.91134071340713	28.634292742927432	21.43330873308733
41	22.03453874538745	25.24575645756458	27.774858548585485	24.944846248462486
42	23.925608856088562	24.290086100861007	28.087380073800738	23.696924969249693
43	23.7040098400984	24.427453874538745	28.370578105781057	23.497958179581797
44	22.76861008610086	25.24870848708487	28.67247232472325	23.31020910209102
45	22.60211562115621	25.82848708487085	27.286789667896677	24.28260762607626
46	23.762460024600244	26.653874538745388	27.19940959409594	22.384255842558424
47	22.558228782287824	26.160885608856088	29.025731857318572	22.255153751537517
48	22.83552275522755	24.693136531365315	29.49431734317343	22.977023370233702
49	22.278376383763838	24.839950799507996	29.6720295202952	23.209643296432965
50	22.310061500615006	24.656334563345634	29.44649446494465	23.58710947109471
51	21.601180811808117	25.424452644526447	27.73431734317343	25.240049200492003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	367.0
1	788.5
2	1210.0
3	4473.5
4	7737.0
5	5157.5
6	2578.0
7	2478.0
8	2378.0
9	2339.5
10	2301.0
11	2257.5
12	2214.0
13	2122.5
14	2031.0
15	2041.0
16	2051.0
17	2075.0
18	2099.0
19	2061.5
20	2024.0
21	2138.0
22	2252.0
23	2458.5
24	2665.0
25	3025.5
26	3832.5
27	4279.0
28	4887.5
29	5496.0
30	6884.5
31	8273.0
32	9726.5
33	11180.0
34	11428.0
35	11676.0
36	12275.0
37	12874.0
38	14795.5
39	16717.0
40	18606.0
41	20495.0
42	24022.5
43	27550.0
44	30222.0
45	32894.0
46	36827.0
47	40760.0
48	40687.0
49	40614.0
50	39730.0
51	38846.0
52	35652.5
53	32459.0
54	30923.0
55	29387.0
56	27793.5
57	26200.0
58	24425.5
59	22651.0
60	22199.5
61	21748.0
62	20002.5
63	18257.0
64	16029.0
65	13801.0
66	11778.5
67	9756.0
68	9138.0
69	8520.0
70	7494.0
71	6468.0
72	5357.5
73	4247.0
74	3549.0
75	2445.0
76	2039.0
77	1753.5
78	1468.0
79	1074.0
80	680.0
81	544.5
82	409.0
83	264.5
84	120.0
85	86.0
86	52.0
87	51.0
88	50.0
89	28.0
90	6.0
91	5.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	508125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.963507165795487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69332053583403	22.08110337946727
2	9.109200616323362	5.4588719588374754
3	3.618074559491804	3.2523060896914506
4	1.995156109042907	2.3912749748075117
5	1.3448465411133772	2.0148159485772976
6	0.967178522956472	1.738803636192585
7	0.7196377206758615	1.5094009000173536
8	0.560486637998833	1.3435316315208523
9	0.48711829698222164	1.3136195323995203
>10	7.20051533680167	50.482983507206114
>50	0.2699723419852896	4.841466882617575
>100	0.030512844548705946	1.681266405027385
>500	0.0013266454151611281	0.3193988784583293
>1k	0.0026532908303222563	1.571156275179274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4079	0.8027552275522756	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	1282	0.25230012300123	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC	1282	0.25230012300123	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG	1262	0.2483640836408364	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	849	0.1670848708487085	Illumina Single End Adapter 1 (95% over 21bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	758	0.14917589175891757	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.968019680196802E-4	0.0	0.0	0.0854120541205412	0.0
2	1.968019680196802E-4	0.0	0.0	0.42469864698646986	0.0
3	1.968019680196802E-4	0.0	0.0	0.5681672816728167	0.0
4	1.968019680196802E-4	0.0	0.0	0.7974415744157441	0.0
5	1.968019680196802E-4	0.0	0.0	1.6029520295202953	0.0
6	1.968019680196802E-4	0.0	0.0	2.074292742927429	0.0
7	3.936039360393604E-4	0.0	0.0	2.5344157441574415	0.0
8	3.936039360393604E-4	0.0	0.0	3.270651906519065	0.0
9	3.936039360393604E-4	0.0	0.0	3.5644772447724478	0.0
10	3.936039360393604E-4	0.0	0.0	4.433751537515375	0.0
11	3.936039360393604E-4	0.0	0.0	5.2876752767527675	0.0
12	3.936039360393604E-4	0.0	0.0	6.237244772447725	0.0
13	5.904059040590406E-4	0.0	0.0	6.515522755227552	0.0
14	5.904059040590406E-4	0.0	0.0	6.632423124231242	0.0
15	5.904059040590406E-4	0.0	0.0	6.88	0.0
16	5.904059040590406E-4	0.0	0.0	7.286789667896679	0.0
17	5.904059040590406E-4	0.0	0.0	7.742189421894219	0.0
18	5.904059040590406E-4	0.0	0.0	8.269421894218942	0.0
19	5.904059040590406E-4	0.0	0.0	8.61480934809348	0.0
20	5.904059040590406E-4	0.0	0.0	8.94760147601476	0.0
21	5.904059040590406E-4	0.0	0.0	9.3169987699877	0.0
22	5.904059040590406E-4	0.0	0.0	9.724182041820418	0.0
23	5.904059040590406E-4	0.0	0.0	10.12309963099631	0.0
24	5.904059040590406E-4	0.0	0.0	10.431881918819188	0.0
25	5.904059040590406E-4	0.0	0.0	10.729052890528905	0.0
26	5.904059040590406E-4	0.0	0.0	10.99670356703567	0.0
27	5.904059040590406E-4	0.0	0.0	11.266912669126691	0.0
28	7.872078720787208E-4	0.0	0.0	11.54479704797048	0.0
29	9.840098400984009E-4	0.0	0.0	11.837441574415744	0.0
30	9.840098400984009E-4	0.0	0.0	12.190897908979089	0.0
31	9.840098400984009E-4	0.0	0.0	12.515227552275523	0.0
32	9.840098400984009E-4	0.0	0.0	12.824009840098402	0.0
33	9.840098400984009E-4	0.0	0.0	13.133185731857319	0.0
34	9.840098400984009E-4	0.0	0.0	13.438031980319803	0.0
35	0.0011808118081180813	0.0	0.0	13.761771217712177	0.0
36	0.0011808118081180813	0.0	0.0	14.060319803198032	0.0
37	0.0011808118081180813	0.0	0.0	14.365953259532596	0.0
38	0.0011808118081180813	0.0	0.0	14.680836408364083	0.0
39	0.0011808118081180813	0.0	0.0	15.04629766297663	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGTA	65	0.0	45.000004	26
GCGACAC	20	7.029567E-4	45.0	35
ATTAGCG	20	7.029567E-4	45.0	1
GCGATAT	45	3.8380676E-10	45.0	9
CATCGTT	40	6.8030204E-9	45.0	36
CCAGTCG	40	6.8030204E-9	45.0	28
CTAGGCG	20	7.029567E-4	45.0	1
AGTAACG	25	3.887723E-5	45.0	1
TACGTAG	20	7.029567E-4	45.0	1
TATAGCG	30	2.1631022E-6	44.999996	1
CGTACAC	75	0.0	42.0	29
CACGCGT	70	0.0	41.785717	25
TACGGGA	135	0.0	41.666668	4
ACGGGTA	60	3.6379788E-12	41.249996	5
CGTTTTT	1885	0.0	40.941643	1
CGTTATT	165	0.0	40.90909	1
CGCAGTA	50	1.0786607E-9	40.5	37
CGCATCG	45	1.9246727E-8	40.0	21
CGATGAA	225	0.0	40.0	19
GCATCGT	45	1.9246727E-8	40.0	35
>>END_MODULE