##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935970.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	869780
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06327002230449	33.0	31.0	34.0	30.0	34.0
2	32.2138529283267	33.0	31.0	34.0	30.0	34.0
3	32.05181655131182	33.0	31.0	34.0	30.0	34.0
4	35.764507116742166	37.0	35.0	37.0	35.0	37.0
5	32.22560072662053	37.0	35.0	37.0	0.0	37.0
6	33.945786290786174	37.0	35.0	37.0	18.0	37.0
7	35.53931683874083	37.0	35.0	37.0	32.0	37.0
8	35.7606509692106	37.0	35.0	37.0	35.0	37.0
9	37.71855756628113	39.0	38.0	39.0	35.0	39.0
10	37.37306560279611	39.0	37.0	39.0	34.0	39.0
11	37.309847317712524	39.0	37.0	39.0	34.0	39.0
12	37.29254294189335	39.0	37.0	39.0	34.0	39.0
13	37.2691439214514	39.0	37.0	39.0	34.0	39.0
14	38.5507933040539	40.0	38.0	41.0	34.0	41.0
15	38.64331785049093	40.0	38.0	41.0	34.0	41.0
16	38.607378877417275	40.0	38.0	41.0	34.0	41.0
17	38.523829014233485	40.0	38.0	41.0	34.0	41.0
18	38.337796914162205	40.0	38.0	41.0	34.0	41.0
19	38.08281059578284	40.0	37.0	41.0	34.0	41.0
20	37.9253765319966	40.0	35.0	41.0	34.0	41.0
21	37.89461932902573	40.0	35.0	41.0	34.0	41.0
22	37.925878957897396	40.0	35.0	41.0	34.0	41.0
23	37.88463864425487	40.0	35.0	41.0	34.0	41.0
24	37.85938398215641	40.0	35.0	41.0	34.0	41.0
25	37.65006208466509	40.0	35.0	41.0	33.0	41.0
26	37.646831382648486	40.0	35.0	41.0	33.0	41.0
27	37.60660856768378	40.0	35.0	41.0	33.0	41.0
28	37.486221803214605	40.0	35.0	41.0	33.0	41.0
29	37.41294695210283	40.0	35.0	41.0	33.0	41.0
30	37.19727057416818	39.0	35.0	41.0	33.0	41.0
31	36.99476649267631	39.0	35.0	41.0	32.0	41.0
32	36.670438501690086	39.0	35.0	41.0	31.0	41.0
33	36.33448458230817	39.0	35.0	41.0	30.0	41.0
34	35.96464163351652	39.0	35.0	41.0	27.0	41.0
35	35.78936972567776	39.0	35.0	41.0	25.0	41.0
36	35.57862218032146	39.0	35.0	41.0	24.0	41.0
37	35.460333647589046	39.0	35.0	41.0	23.0	41.0
38	35.324462507760586	39.0	35.0	41.0	23.0	41.0
39	35.246101312975696	39.0	35.0	41.0	22.0	41.0
40	35.21590517142266	39.0	35.0	41.0	22.0	41.0
41	35.1114097817839	39.0	35.0	41.0	22.0	41.0
42	35.0715123364529	39.0	35.0	41.0	21.0	41.0
43	34.975782381751706	39.0	35.0	41.0	21.0	41.0
44	34.935481386097635	39.0	35.0	41.0	21.0	41.0
45	34.89915610844122	39.0	35.0	41.0	20.0	41.0
46	34.777708156085446	38.0	35.0	41.0	20.0	41.0
47	34.63862585941273	38.0	34.0	41.0	20.0	41.0
48	34.59697969601508	38.0	34.0	41.0	20.0	41.0
49	34.540388374071604	38.0	34.0	41.0	20.0	41.0
50	34.455017360711906	38.0	34.0	40.0	20.0	41.0
51	33.53312446825634	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	17.0
9	33.0
10	36.0
11	28.0
12	18.0
13	20.0
14	21.0
15	60.0
16	86.0
17	178.0
18	378.0
19	712.0
20	1382.0
21	2576.0
22	4047.0
23	5944.0
24	8508.0
25	12684.0
26	16839.0
27	17897.0
28	16146.0
29	15110.0
30	15318.0
31	17120.0
32	20700.0
33	28848.0
34	47187.0
35	62979.0
36	62200.0
37	92308.0
38	153579.0
39	266632.0
40	186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.313527558693	17.543861666168457	21.38575271907839	20.756858056060153
2	37.68585159465612	23.929039527236775	22.912115707420266	15.472993170686841
3	28.41488652302881	24.00377106854607	32.48568603554922	15.095656372875899
4	24.912161696061073	23.32773804870197	35.138540780427235	16.62155947480972
5	22.60088758076755	34.23061003932028	27.602382211593735	15.566120168318426
6	22.508105497942008	33.19609556439559	30.487364620938628	13.808434316723769
7	72.87762422681597	4.866747913265423	18.379130354802363	3.8764975051162365
8	74.40375727195384	6.535560716503023	15.434707627216076	3.6259743843270713
9	69.06470601761366	6.3589643358090555	16.875991630067375	7.700338016509923
10	36.97969601508427	26.235139920439654	23.64172549380303	13.143438570673045
11	27.6495205684196	23.256800570259145	32.44153694037573	16.652141920945525
12	24.11287911885764	20.53312331854032	35.098760606130284	20.25523695647175
13	23.335096231230885	21.29837430154752	37.42245165444135	17.944077812780243
14	19.543677711605234	26.0787785417002	35.432983053185865	18.944560693508702
15	18.9260502655844	23.48720366069581	38.602060291108096	18.984685782611695
16	21.875531743659316	23.2355308238865	35.17590655108188	19.7130308813723
17	21.02531674676355	24.108510197981097	34.98263928809584	19.883533767159513
18	21.928648623789922	23.26599829842029	34.49607946837131	20.309273609418472
19	21.843684609901352	25.17395203384764	32.84106325737543	20.14130009887558
20	24.406631562004186	24.478028926855067	33.22805766975557	17.887281841385178
21	22.661707558233115	25.915863781645932	33.909839269700385	17.512589390420565
22	21.618915127963394	22.52500632343811	34.73073650808251	21.125342040515992
23	21.599484927222974	24.87594564142657	33.96399089424912	19.560578537101335
24	21.456690197521212	24.073673802570767	34.18657591574881	20.283060084159214
25	20.53680240980478	26.834946768148267	32.27873715192347	20.34951367012348
26	20.65579801788958	24.814435834348917	34.40927590885051	20.12049023891099
27	22.70585665340661	23.958932143760492	32.99466531766654	20.340545885166364
28	19.74993676561889	25.993584584607603	34.4518154015958	19.8046632481777
29	21.92025569684288	23.920761571891745	34.21451401503828	19.944468716227092
30	22.58490652808756	24.465497022235507	34.05757777828876	18.892018671388165
31	22.297477523051807	23.87523281749408	32.744717054887445	21.08257260456667
32	23.578606084297178	25.41769183011796	32.03016854836855	18.97353353721631
33	22.850260985536572	24.593460415277427	31.948423739336384	20.607854859849617
34	21.551886683989053	25.56887948676677	32.64883073880752	20.230403090436663
35	22.329324656809767	25.61866219043896	31.22387270344225	20.82814044930902
36	21.958541240313643	28.041573731288373	30.560256616615693	19.43962841178229
37	21.673526638920187	27.967417047989144	30.90988525834119	19.449171054749474
38	21.25675458161834	26.766998551357812	30.365954609211528	21.61029225781232
39	21.753661845524157	26.836211455770425	30.64269125525995	20.76743544344547
40	21.42771735381361	25.10876313550553	32.126974637264595	21.336544873416265
41	20.309273609418472	26.596725608774634	31.018188507438666	22.075812274368232
42	21.49187150773759	26.637885442295755	30.098875577732297	21.771367472234356
43	22.060521051300327	26.224907447860378	30.03173216215595	21.682839338683348
44	21.841845064269126	25.948860631424036	30.458161834027	21.751132470279842
45	21.105336981765504	25.050817448090324	30.570489089194968	23.273356480949207
46	22.709535744671065	26.68835797558003	29.464347306215366	21.137758973533536
47	20.789509991032215	26.1929453425004	32.334038492492354	20.683506173975026
48	21.43093655867001	25.74524592425671	30.716847938559177	22.106969578514107
49	21.048196095564396	25.16222493044218	31.70870794913656	22.08087102485686
50	20.729724757984776	24.987008208972384	31.684218997907514	22.599048035135322
51	20.365839637609508	24.98401894732001	30.721331831037734	23.928809584032745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	538.0
1	916.0
2	1294.0
3	13382.0
4	25470.0
5	17014.5
6	8559.0
7	8213.0
8	7867.0
9	7890.5
10	7914.0
11	7744.0
12	7574.0
13	7351.0
14	7128.0
15	6780.0
16	6432.0
17	6045.0
18	5658.0
19	5443.5
20	5229.0
21	5278.0
22	5327.0
23	5406.0
24	5485.0
25	5869.0
26	7419.0
27	8585.0
28	9670.0
29	10755.0
30	12922.0
31	15089.0
32	16559.0
33	18029.0
34	20472.5
35	22916.0
36	24640.0
37	26364.0
38	29676.5
39	32989.0
40	35653.5
41	38318.0
42	42997.0
43	47676.0
44	61987.0
45	76298.0
46	72577.0
47	68856.0
48	68651.5
49	68447.0
50	62276.5
51	56106.0
52	52817.5
53	49529.0
54	46235.5
55	42942.0
56	41082.5
57	39223.0
58	36903.0
59	34583.0
60	32364.5
61	30146.0
62	27746.5
63	25347.0
64	22145.5
65	18944.0
66	15372.5
67	11801.0
68	10105.5
69	8410.0
70	7267.0
71	6124.0
72	5080.0
73	4036.0
74	3549.0
75	2578.0
76	2094.0
77	1597.0
78	1100.0
79	841.0
80	582.0
81	498.5
82	415.0
83	284.0
84	153.0
85	109.5
86	66.0
87	48.5
88	31.0
89	19.5
90	8.0
91	6.5
92	5.0
93	11.5
94	18.0
95	10.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	869780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.15073262962667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.31455051523615	26.122108956519508
2	8.933284326992952	6.2802297776512725
3	3.6744705951945233	3.8748100034132356
4	2.160257822747431	3.0373858055381753
5	1.4523716723307278	2.552596416647058
6	1.0512929773891566	2.217223101816624
7	0.8508411956351771	2.0935383964630794
8	0.6994389833194339	1.966863415471946
9	0.5769595978704121	1.8252497306555797
>10	6.184257712967511	42.30580853930111
>50	0.0728168844713078	1.6503386559354287
>100	0.02349997556189293	1.5424868058348549
>500	0.0036408412842334707	0.9276095416288141
>1k	0.0016549278564697595	1.3702979030859368
>5k	3.309855712939519E-4	0.645359353745771
>10k+	3.309855712939519E-4	1.5880935962916485
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13650	1.5693623674952286	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	5547	0.6377474763733358	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	3525	0.4052748971004162	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	3520	0.4047000390903447	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1629	0.18728873968129872	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1565	0.17993055715238337	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	1539	0.1769412955000115	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	894	0.10278461220078641	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	875	0.10060015176251466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2994320402860494E-4	0.0	0.0	0.07542137092138242	0.0
2	2.2994320402860494E-4	0.0	0.0	0.4072294143346593	0.0
3	2.2994320402860494E-4	0.0	0.0	0.6208466508772333	0.0
4	2.2994320402860494E-4	0.0	0.0	0.9639219112879119	0.0
5	3.449148060429074E-4	0.0	0.0	2.054772471199614	0.0
6	3.449148060429074E-4	0.0	0.0	2.7842672859803628	0.0
7	3.449148060429074E-4	0.0	0.0	3.3882131113614937	0.0
8	3.449148060429074E-4	0.0	0.0	4.215318816252386	0.0
9	3.449148060429074E-4	0.0	0.0	4.656464853181264	0.0
10	3.449148060429074E-4	0.0	0.0	5.787440502195958	0.0
11	3.449148060429074E-4	0.0	0.0	6.542573984225896	0.0
12	3.449148060429074E-4	0.0	0.0	7.615948860631424	0.0
13	3.449148060429074E-4	0.0	0.0	7.984547816689278	0.0
14	3.449148060429074E-4	0.0	0.0	8.186208006622364	0.0
15	4.598864080572099E-4	0.0	0.0	8.440065303869945	0.0
16	4.598864080572099E-4	0.0	0.0	8.767964312814735	0.0
17	4.598864080572099E-4	0.0	0.0	9.15530364000092	0.0
18	4.598864080572099E-4	0.0	0.0	9.563682770355722	0.0
19	4.598864080572099E-4	0.0	0.0	9.940559681758605	0.0
20	4.598864080572099E-4	0.0	0.0	10.223734737519832	0.0
21	5.748580100715123E-4	0.0	0.0	10.54324081951758	0.0
22	5.748580100715123E-4	0.0	0.0	10.917703327278163	0.0
23	5.748580100715123E-4	0.0	0.0	11.274690151532571	0.0
24	5.748580100715123E-4	0.0	0.0	11.570052197107314	0.0
25	5.748580100715123E-4	0.0	0.0	11.846328956747683	0.0
26	5.748580100715123E-4	0.0	0.0	12.114097817838994	0.0
27	5.748580100715123E-4	0.0	0.0	12.408540090597622	0.0
28	6.898296120858149E-4	0.0	0.0	12.690680401940721	0.0
29	6.898296120858149E-4	0.0	0.0	12.996504863298766	0.0
30	6.898296120858149E-4	0.0	0.0	13.354296488767275	0.0
31	6.898296120858149E-4	0.0	0.0	13.658856262503162	0.0
32	8.048012141001173E-4	0.0	0.0	13.977557543286808	0.0
33	8.048012141001173E-4	0.0	0.0	14.290050357561682	0.0
34	8.048012141001173E-4	0.0	0.0	14.59300052886937	0.0
35	8.048012141001173E-4	0.0	0.0	14.905378371542229	0.0
36	8.048012141001173E-4	0.0	0.0	15.229023431212491	0.0
37	9.197728161144198E-4	0.0	0.0	15.555772724137139	0.0
38	9.197728161144198E-4	0.0	0.0	15.910575087953276	0.0
39	9.197728161144198E-4	0.0	0.0	16.26342293453517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	25	3.8900194E-5	45.000004	40
CGCGCAA	25	3.8900194E-5	45.000004	18
CATACCG	20	7.032338E-4	45.000004	1
CTCGACG	25	3.8900194E-5	45.000004	1
CCGCGTA	20	7.032338E-4	45.000004	40
CGTTATT	1040	0.0	44.134613	1
CGTTTTT	7875	0.0	43.285713	1
CCCATCG	180	0.0	42.500004	40
CGATCAC	75	0.0	42.0	34
CCGATGA	1025	0.0	41.707317	18
CGATGAA	1040	0.0	41.322113	19
TCTACGG	40	3.4569348E-7	39.375004	2
TATAGCG	40	3.4569348E-7	39.375004	1
TACGGCT	520	0.0	39.375	7
ACGGGAC	260	0.0	38.942307	5
TAGCCGT	35	6.246535E-6	38.57143	44
ATAACGG	70	0.0	38.57143	2
GCTAACG	35	6.246535E-6	38.57143	1
AAGGGCG	490	0.0	37.65306	5
GCGAATC	30	1.13969334E-4	37.500004	17
>>END_MODULE