##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935969.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441630
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03605733306161	33.0	31.0	34.0	30.0	34.0
2	32.15746892194824	33.0	31.0	34.0	30.0	34.0
3	32.222262980322895	34.0	31.0	34.0	30.0	34.0
4	35.78271856531486	37.0	35.0	37.0	35.0	37.0
5	35.809521545184886	37.0	35.0	37.0	35.0	37.0
6	35.824045015057855	37.0	35.0	37.0	35.0	37.0
7	35.99672576591264	37.0	35.0	37.0	35.0	37.0
8	35.589529696805016	37.0	35.0	37.0	33.0	37.0
9	37.39758168602676	39.0	37.0	39.0	34.0	39.0
10	37.13558861490388	39.0	37.0	39.0	34.0	39.0
11	37.1064692163123	39.0	37.0	39.0	34.0	39.0
12	36.965466567035755	39.0	37.0	39.0	33.0	39.0
13	36.91036614360438	39.0	37.0	39.0	33.0	39.0
14	38.05280211942124	40.0	37.0	41.0	33.0	41.0
15	38.1397391481557	40.0	37.0	41.0	33.0	41.0
16	38.28706609605326	40.0	37.0	41.0	34.0	41.0
17	38.18447116364377	40.0	37.0	41.0	34.0	41.0
18	38.17823517424088	40.0	37.0	41.0	34.0	41.0
19	38.21024160496343	40.0	37.0	41.0	34.0	41.0
20	38.02334533432964	40.0	36.0	41.0	34.0	41.0
21	38.05765006906234	40.0	36.0	41.0	34.0	41.0
22	38.02735547856803	40.0	36.0	41.0	34.0	41.0
23	38.02733283517877	40.0	36.0	41.0	34.0	41.0
24	37.92930054570568	40.0	36.0	41.0	34.0	41.0
25	37.8836107148518	40.0	36.0	41.0	33.0	41.0
26	37.72147951905441	40.0	36.0	41.0	33.0	41.0
27	37.67284831193533	40.0	36.0	41.0	33.0	41.0
28	37.66644702579082	40.0	36.0	41.0	33.0	41.0
29	37.6752779476032	40.0	36.0	41.0	33.0	41.0
30	37.55214772547155	40.0	36.0	41.0	33.0	41.0
31	37.58905192129158	40.0	36.0	41.0	33.0	41.0
32	37.50340330140615	40.0	35.0	41.0	33.0	41.0
33	37.45602427371329	40.0	35.0	41.0	33.0	41.0
34	37.35723795937776	40.0	35.0	41.0	33.0	41.0
35	37.34868555125331	40.0	35.0	41.0	33.0	41.0
36	37.29956071824831	40.0	35.0	41.0	33.0	41.0
37	37.294724090301834	40.0	35.0	41.0	33.0	41.0
38	37.19722165613749	40.0	35.0	41.0	32.0	41.0
39	37.13492742793741	40.0	35.0	41.0	32.0	41.0
40	37.041817811289995	39.0	35.0	41.0	32.0	41.0
41	36.95100423431379	39.0	35.0	41.0	32.0	41.0
42	36.98405226094242	39.0	35.0	41.0	32.0	41.0
43	36.93465797160519	39.0	35.0	41.0	32.0	41.0
44	36.882381178814846	39.0	35.0	41.0	32.0	41.0
45	36.763987953716914	39.0	35.0	41.0	31.0	41.0
46	36.689131173153996	39.0	35.0	41.0	31.0	41.0
47	36.599719221973146	39.0	35.0	41.0	31.0	41.0
48	36.59132305323461	39.0	35.0	41.0	31.0	41.0
49	36.57330117972058	39.0	35.0	41.0	31.0	41.0
50	36.46005253266309	39.0	35.0	41.0	31.0	41.0
51	35.53435002151122	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	9.0
10	19.0
11	11.0
12	13.0
13	14.0
14	13.0
15	29.0
16	37.0
17	66.0
18	104.0
19	167.0
20	396.0
21	642.0
22	1024.0
23	1422.0
24	1824.0
25	2400.0
26	3047.0
27	3632.0
28	4314.0
29	4931.0
30	6130.0
31	7935.0
32	10360.0
33	14606.0
34	29184.0
35	36377.0
36	30378.0
37	44750.0
38	82433.0
39	155219.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.602653805221564	20.493173018137355	23.069537848425153	10.834635328215928
2	31.49401082354007	27.50741570998347	23.0428186490954	17.955754817381063
3	31.489935013472813	26.929103548219096	24.69940900754025	16.881552430767837
4	28.98897266942916	23.859565699793944	28.562597649616194	18.5888639811607
5	28.401150284174538	27.52168104521885	22.980096460838258	21.09707220976836
6	25.385050834408894	35.83225777234336	23.40556574508072	15.377125648167016
7	76.38543577202636	6.016348527047528	12.155197790005207	5.443017910920907
8	75.24986980051173	8.530896904648689	9.93410773724611	6.28512555759346
9	70.02445486040351	7.399859610986573	11.157530059099246	11.418155469510676
10	41.28523877453977	25.188506215610353	18.625093403980706	14.901161605869168
11	32.60580123632905	23.731404116568168	23.582410615220887	20.08038403188189
12	29.97984738355637	19.965582048320993	28.050857052283583	22.003713515839053
13	26.532391368340015	22.140253153091955	29.907614971808982	21.41974050675905
14	20.088535652016393	28.31963408282952	27.673165319385006	23.918664945769084
15	19.119398591581188	25.295835880714623	34.43561352263207	21.149152005072118
16	22.18984217557684	24.997169576342188	30.24930371578018	22.563684532300794
17	20.91049068224532	25.748250798179473	28.581391662704075	24.759866856871138
18	22.2419219708806	26.58152752303965	28.52433032176256	22.65222018431719
19	23.272422616217195	27.97590743382469	26.370944002898355	22.38072594705976
20	26.849172384122454	26.446799356927748	28.108371261010344	18.595656997939454
21	24.33258610148767	28.59022258451645	27.028960894866742	20.048230419129133
22	21.59386817018771	24.385571632361934	28.68690985666735	25.33365034078301
23	23.49908294273487	26.91891402305097	27.830084006974165	21.751919027239996
24	24.603627470959854	23.864773679324323	27.347100514004936	24.184498335710888
25	21.825736476235765	27.85363313180717	25.40022190521477	24.920408486742296
26	21.162058736951746	25.801236329053733	28.320313384507394	24.716391549487128
27	22.44684464370627	26.759278128750314	27.091456649231255	23.70242057831216
28	18.636415098611963	27.69807304757376	30.14899350134728	23.516518352466996
29	24.09030183637887	24.444670878337067	28.19894481806037	23.266082467223693
30	22.204560378597467	25.968344541811017	28.24423159658538	23.582863483006136
31	22.749813192038584	26.49752054887576	26.48846319317075	24.264203065914906
32	24.866064352512286	27.32490999252768	26.296900120009965	21.512125534950073
33	22.393179811154134	27.81876231234291	25.150012453864097	24.63804542263886
34	22.447976813169397	26.66779883612979	28.086633607318344	22.79759074338247
35	20.932681203722574	25.611484727033947	25.850146049860744	27.60568801938274
36	21.42041980843693	30.95668319634083	25.342934130380634	22.27996286484161
37	20.28258949799606	27.11636437742001	29.390213527160746	23.21083259742318
38	19.772886805697077	28.307859520413015	27.056359395874374	24.86289427801553
39	22.932318909494374	27.12927110929964	26.908950931775465	23.02945904943052
40	21.477254715485813	25.84290016529674	28.765255983515615	23.91458913570183
41	18.94459162647465	28.91832529493015	26.1236781921518	26.013404886443404
42	20.811539071168173	25.394561057899146	27.923827638520933	25.870072232411744
43	22.77336231687159	25.13959649480334	27.32921223648756	24.75782895183751
44	21.666553449720354	24.388968140751306	27.924280506306182	26.020197903222154
45	22.322079568869867	23.397414124946224	27.157348911985146	27.123157394198767
46	23.381337318569845	26.547336005253264	26.271765957928583	23.79956071824831
47	19.587437447637164	25.38935307836877	31.51303127052057	23.510178203473494
48	19.926182551004235	25.0406448837262	28.81620360935625	26.216968955913323
49	20.455584991961597	23.29461313769445	31.27708715440527	24.972714715938682
50	19.863007494961845	23.39696125716097	30.003170074496747	26.736861173380433
51	18.72563005230623	23.2923487987682	28.07123610261984	29.910785046305733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	406.0
1	598.5
2	791.0
3	1188.0
4	1585.0
5	1132.5
6	680.0
7	688.0
8	696.0
9	669.5
10	643.0
11	682.0
12	721.0
13	723.5
14	726.0
15	754.5
16	783.0
17	783.5
18	784.0
19	830.0
20	876.0
21	851.5
22	827.0
23	1107.5
24	1388.0
25	1479.5
26	2117.5
27	2664.0
28	3347.5
29	4031.0
30	4912.5
31	5794.0
32	6702.0
33	7610.0
34	8474.0
35	9338.0
36	10181.0
37	11024.0
38	12184.0
39	13344.0
40	15702.0
41	18060.0
42	20839.0
43	23618.0
44	35771.0
45	47924.0
46	47486.0
47	47048.0
48	47089.0
49	47130.0
50	41668.5
51	36207.0
52	32010.5
53	27814.0
54	25817.5
55	23821.0
56	22363.0
57	20905.0
58	20901.0
59	20897.0
60	20090.5
61	19284.0
62	17490.5
63	15697.0
64	13372.5
65	11048.0
66	8740.5
67	6433.0
68	5251.5
69	4070.0
70	3094.5
71	2119.0
72	1827.5
73	1536.0
74	1210.0
75	686.0
76	488.0
77	317.5
78	147.0
79	128.5
80	110.0
81	86.0
82	62.0
83	38.5
84	15.0
85	13.0
86	11.0
87	12.5
88	14.0
89	8.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.435538143367808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54291493264758	22.17554493109594
2	11.855770452998481	7.453850485884937
3	4.380851745351291	4.131432964242898
4	2.183582906608998	2.7456841499965243
5	1.2607562460312405	1.9816275530802137
6	0.8452426159240608	1.5942393895968476
7	0.652521121910266	1.4358646832014326
8	0.5604760284504875	1.409509245663888
9	0.5145248330199163	1.4556928512696776
>10	7.0141615661750105	45.55928588082659
>50	0.12079535940971653	2.5231238810104593
>100	0.05966999681684792	3.176436827897664
>500	0.005093780216072383	1.0970899486243122
>1k	0.0036384144400517025	3.260617207608621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	3923	0.8883001607680638	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	3505	0.7936507936507936	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	3410	0.7721395738514141	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2179	0.49339945202997987	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1237	0.28009872517718454	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	927	0.2099042184634196	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT	805	0.18227928356316375	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	693	0.15691868758915833	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	659	0.1492199352399067	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	586	0.1326902610782782	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA	578	0.1308787899372778	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	548	0.12408577315852637	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	475	0.10755609899689784	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5286778525009626E-4	0.0	0.0	0.2160179335642959	0.0
2	4.5286778525009626E-4	0.0	0.0	1.2503679550755158	0.0
3	4.5286778525009626E-4	0.0	0.0	1.7050472114666124	0.0
4	4.5286778525009626E-4	0.0	0.0	2.4026900346443854	0.0
5	4.5286778525009626E-4	0.0	0.0	4.785680320630392	0.0
6	4.5286778525009626E-4	0.0	0.0	5.707945565292213	0.0
7	4.5286778525009626E-4	0.0	0.0	6.7219165364671785	0.0
8	4.5286778525009626E-4	0.0	0.0	8.110635599936598	0.0
9	4.5286778525009626E-4	0.0	0.0	8.571881439213822	0.0
10	4.5286778525009626E-4	0.0	0.0	10.392409935919208	0.0
11	4.5286778525009626E-4	0.0	0.0	12.061001290673188	0.0
12	4.5286778525009626E-4	0.0	0.0	14.251296334035278	0.0
13	4.5286778525009626E-4	0.0	0.0	14.824400516269275	0.0
14	4.5286778525009626E-4	0.0	0.0	15.073477798156828	0.0
15	6.793016778751444E-4	0.0	0.0	15.650204922672826	0.0
16	6.793016778751444E-4	0.0	0.0	16.51744673142676	0.0
17	6.793016778751444E-4	0.0	0.0	17.510359350587596	0.0
18	6.793016778751444E-4	0.0	0.0	18.533161243574938	0.0
19	6.793016778751444E-4	0.0	0.0	19.38591128320087	0.0
20	6.793016778751444E-4	0.0	0.0	20.039399497316758	0.0
21	6.793016778751444E-4	0.0	0.0	20.86927971378756	0.0
22	6.793016778751444E-4	0.0	0.0	21.70980232321174	0.0
23	6.793016778751444E-4	0.0	0.0	22.507528926929783	0.0
24	6.793016778751444E-4	0.0	0.0	23.162375744401423	0.0
25	6.793016778751444E-4	0.0	0.0	23.747028055159298	0.0
26	6.793016778751444E-4	0.0	0.0	24.28594071960691	0.0
27	6.793016778751444E-4	0.0	0.0	24.839118719289903	0.0
28	9.057355705001925E-4	0.0	0.0	25.372823404207143	0.0
29	9.057355705001925E-4	0.0	0.0	25.921246292145007	0.0
30	9.057355705001925E-4	0.0	0.0	26.52197540927926	0.0
31	0.0011321694631252406	0.0	0.0	27.097117496546883	0.0
32	0.0011321694631252406	0.0	0.0	27.657088513008627	0.0
33	0.0011321694631252406	0.0	0.0	28.21660666168512	0.0
34	0.0011321694631252406	0.0	0.0	28.75302855331386	0.0
35	0.0011321694631252406	0.0	0.0	29.338586599642234	0.0
36	0.0013586033557502887	0.0	0.0	29.87749926408985	0.0
37	0.0013586033557502887	0.0	0.0	30.441546090618843	0.0
38	0.0013586033557502887	0.0	0.0	30.995176958087086	0.0
39	0.0013586033557502887	0.0	0.0	31.539750469850325	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAT	30	2.1624546E-6	45.000004	7
GTTTCGA	20	7.028562E-4	45.000004	28
GACGTTG	20	7.028562E-4	45.000004	1
TACGGGT	20	7.028562E-4	45.000004	4
ATCCGGC	30	2.1624546E-6	45.000004	6
TGCGACG	20	7.028562E-4	45.000004	1
AACGATA	20	7.028562E-4	45.000004	1
GCGCTAG	20	7.028562E-4	45.000004	1
GCGTAAG	30	2.1624546E-6	45.000004	1
TGGTCGA	30	2.1624546E-6	45.000004	6
ACGACGG	30	2.1624546E-6	45.000004	27
TACGTAG	20	7.028562E-4	45.000004	1
GTAGCAC	25	3.886891E-5	45.0	9
TACTCGC	25	3.886891E-5	45.0	45
GGCGATT	100	0.0	45.0	8
TATACGG	35	1.2096643E-7	45.0	2
CGCCGGT	55	6.002665E-11	40.909092	28
CGATGAA	260	0.0	40.673077	19
CCGCTTA	50	1.0786607E-9	40.5	41
CGTTAGG	50	1.0786607E-9	40.5	2
>>END_MODULE