##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935968.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493953
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22330464639348	33.0	31.0	34.0	30.0	34.0
2	32.3389128115428	34.0	31.0	34.0	30.0	34.0
3	32.3821254248886	34.0	31.0	34.0	30.0	34.0
4	35.89867457025264	37.0	35.0	37.0	35.0	37.0
5	35.94474575516294	37.0	35.0	37.0	35.0	37.0
6	35.96045170289481	37.0	35.0	37.0	35.0	37.0
7	36.11112393284381	37.0	35.0	37.0	35.0	37.0
8	35.842855494348655	37.0	35.0	37.0	35.0	37.0
9	37.58067467957478	39.0	38.0	39.0	35.0	39.0
10	37.30891400598843	39.0	37.0	39.0	34.0	39.0
11	37.25945585916069	39.0	37.0	39.0	34.0	39.0
12	37.08098138891757	39.0	37.0	39.0	34.0	39.0
13	37.00053243932115	39.0	37.0	39.0	33.0	39.0
14	38.08264146588846	40.0	37.0	41.0	33.0	41.0
15	38.22012418185536	40.0	37.0	41.0	33.0	41.0
16	38.362552712505035	40.0	37.0	41.0	34.0	41.0
17	38.37219330584084	40.0	37.0	41.0	34.0	41.0
18	38.36110115739756	40.0	37.0	41.0	34.0	41.0
19	38.37428864689556	40.0	37.0	41.0	34.0	41.0
20	38.24902571702166	40.0	37.0	41.0	34.0	41.0
21	38.189700234637705	40.0	37.0	41.0	34.0	41.0
22	38.18962127975738	40.0	37.0	41.0	34.0	41.0
23	38.23344326282055	40.0	37.0	41.0	34.0	41.0
24	38.13743412834825	40.0	36.0	41.0	34.0	41.0
25	38.060602931857886	40.0	36.0	41.0	34.0	41.0
26	37.90318107188336	40.0	36.0	41.0	34.0	41.0
27	37.88413067640039	40.0	36.0	41.0	34.0	41.0
28	37.884877711037284	40.0	36.0	41.0	34.0	41.0
29	37.86782953033993	40.0	36.0	41.0	34.0	41.0
30	37.7625685034811	40.0	36.0	41.0	33.0	41.0
31	37.800124708221226	40.0	36.0	41.0	34.0	41.0
32	37.74351608351402	40.0	36.0	41.0	34.0	41.0
33	37.71723423078714	40.0	36.0	41.0	33.0	41.0
34	37.63325255641731	40.0	35.0	41.0	33.0	41.0
35	37.625462341558816	40.0	35.0	41.0	33.0	41.0
36	37.56001077025547	40.0	35.0	41.0	33.0	41.0
37	37.53075899933799	40.0	35.0	41.0	33.0	41.0
38	37.448455622296045	40.0	35.0	41.0	33.0	41.0
39	37.38982048899389	40.0	35.0	41.0	33.0	41.0
40	37.28291355655295	39.0	35.0	41.0	33.0	41.0
41	37.18796525175472	39.0	35.0	41.0	33.0	41.0
42	37.16541452324412	39.0	35.0	41.0	33.0	41.0
43	37.10903668972554	39.0	35.0	41.0	33.0	41.0
44	37.014924496865085	39.0	35.0	41.0	33.0	41.0
45	36.931110854676454	39.0	35.0	41.0	32.0	41.0
46	36.876338437057775	39.0	35.0	41.0	32.0	41.0
47	36.84904434227548	39.0	35.0	41.0	32.0	41.0
48	36.78852036529791	39.0	35.0	41.0	32.0	41.0
49	36.749518678902646	39.0	35.0	41.0	32.0	41.0
50	36.643010569831546	39.0	35.0	41.0	32.0	41.0
51	35.72890133271789	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	12.0
10	21.0
11	6.0
12	19.0
13	12.0
14	17.0
15	38.0
16	42.0
17	51.0
18	120.0
19	156.0
20	291.0
21	433.0
22	681.0
23	1012.0
24	1390.0
25	2067.0
26	2799.0
27	3515.0
28	3967.0
29	4716.0
30	5937.0
31	7769.0
32	10456.0
33	15776.0
34	33605.0
35	42445.0
36	34034.0
37	52126.0
38	90814.0
39	179457.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91298969740036	21.194526604757943	24.272754695284775	11.619729002556923
2	33.5355792960059	23.92838994803149	23.4566851502066	19.07934560575601
3	33.347302273698105	23.90146430935737	25.17830643806192	17.572926978882606
4	29.25501009205329	26.036080355823326	24.971606610345518	19.737302941777862
5	26.61488036311147	29.54876273653566	23.30788556806012	20.52847133229275
6	27.529339835976295	33.58659629559897	23.304039048249532	15.580024820175197
7	80.60827649594192	6.099568177539159	8.315568485260744	4.976586841258176
8	80.27322437559849	5.575226792832517	8.43076163116734	5.720787200401658
9	74.25058659427111	7.814306219417637	10.827750818397702	7.107356367913546
10	43.08851246980988	24.848113079584493	17.670709561436006	14.392664889169618
11	32.96690170927194	24.550513915291535	22.863308857320433	19.619275518116098
12	30.928853554892875	21.881231615153666	27.22182069954024	19.968094130413217
13	27.164325350792485	24.00370075695461	28.55757531587013	20.274398576382772
14	21.06334003437574	27.356853789733034	28.819341111401286	22.76046506448994
15	19.483230185867885	26.34845825412539	34.215401060424774	19.952910499581943
16	23.67512698576585	25.25705886997346	30.330618500140698	20.73719564411999
17	22.51995635212257	25.931616975704163	26.678449164191736	24.869977507981528
18	24.175579457964623	25.41719556314062	29.128277386714927	21.278947592179822
19	25.46092441993469	27.164325350792485	26.11058137110211	21.264168858170716
20	27.305836790139953	26.494018661694536	26.568317228562233	19.631827319603282
21	24.86734567863744	26.479847272918676	28.485503681524353	20.167303366919526
22	23.06474502634866	24.682712727729157	27.590479256123558	24.662062989798624
23	22.51671717754523	25.9324267693485	28.780471016473225	22.77038503663304
24	24.019694181430218	24.700528187904517	27.757904092089735	23.521873538575534
25	23.440691725730993	26.391579765686206	26.436928209768944	23.730800298813858
26	21.455077709822596	26.872192293598786	27.350375440578357	24.322354556000267
27	22.254344036780825	27.662955787291505	27.80426477822789	22.27843539769978
28	19.72556093393501	27.13638747006294	29.421625134375134	23.716426461626916
29	22.458614483564226	25.5969697521829	28.94222729692906	23.002188467323816
30	22.857032956576838	25.8370735677281	28.490564891801444	22.81532858389361
31	22.719772933862128	26.754569766759182	27.659311715892	22.866345583486687
32	25.33277457571874	26.65051128346219	25.803872028310387	22.212842112508678
33	24.154524823211926	26.716104568653293	25.157049354898138	23.972321253236643
34	20.441823412348946	24.738386040777158	31.102149394780476	23.717641152093417
35	21.468236856543033	25.617821938524514	27.704660159974736	25.20928104495772
36	21.921923745781484	28.82642680578921	26.708613977443196	22.543035470986105
37	22.029828748889063	27.415158932125124	29.102363990096226	21.45264832888959
38	22.66875593426905	27.294499679119266	25.7479962668513	24.28874811976038
39	22.85804519863226	24.494435705421367	27.663765580935838	24.983753515010537
40	23.20342218794096	23.255046532767288	30.21502045741194	23.326510821879815
41	19.034806955317613	25.706696790990236	28.85476958334093	26.40372667035123
42	20.92284083708369	25.716009417900082	29.09710033140805	24.264049413608177
43	22.0739625025053	24.860462432660597	28.7189266995038	24.346648365330303
44	21.757738084392646	24.557599609679464	28.19357307274174	25.491089233186155
45	22.462258554963732	23.20079035859687	27.28518705220942	27.051764034229976
46	22.256975866124915	24.592218287974767	27.543308776341068	25.607497069559248
47	19.635876287824956	25.55648006996617	30.586715740161512	24.22092790204736
48	20.4102414602199	25.38541116260049	29.195895156016867	25.00845222116274
49	22.0727478120388	23.76946794533083	30.24720975477424	23.91057448785613
50	20.26974226292785	23.907335313278793	29.990910066342348	25.832012357451013
51	19.869704202626565	23.16131291843556	27.575700522114456	29.393282356823423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	260.0
1	595.5
2	931.0
3	1190.5
4	1450.0
5	1108.0
6	766.0
7	744.5
8	723.0
9	721.0
10	719.0
11	765.0
12	811.0
13	850.0
14	889.0
15	899.0
16	909.0
17	954.0
18	999.0
19	1096.0
20	1193.0
21	1206.5
22	1220.0
23	1284.5
24	1349.0
25	1903.0
26	3325.5
27	4194.0
28	4366.5
29	4539.0
30	5453.5
31	6368.0
32	7474.5
33	8581.0
34	10290.0
35	11999.0
36	12196.5
37	12394.0
38	14597.0
39	16800.0
40	18893.5
41	20987.0
42	24178.5
43	27370.0
44	33246.0
45	39122.0
46	44692.5
47	50263.0
48	51130.5
49	51998.0
50	45669.5
51	39341.0
52	35792.0
53	32243.0
54	30070.0
55	27897.0
56	26238.5
57	24580.0
58	23470.0
59	22360.0
60	21566.5
61	20773.0
62	19589.0
63	18405.0
64	15776.5
65	13148.0
66	11433.5
67	9719.0
68	7831.5
69	5944.0
70	5106.5
71	4269.0
72	3535.5
73	2802.0
74	2149.5
75	1169.0
76	841.0
77	663.0
78	485.0
79	323.5
80	162.0
81	136.5
82	111.0
83	87.5
84	64.0
85	37.5
86	11.0
87	9.5
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	493953.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.97258366440276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.79676701869728	20.919894389727315
2	11.662047881701234	6.990834116651223
3	4.284908070069639	3.8528929687331037
4	2.1974122356948875	2.634484883181893
5	1.3472666530441468	2.0190531238312786
6	0.958707404614448	1.724096273669338
7	0.7017054403234141	1.4722347512502074
8	0.5913907777381538	1.4180407651290423
9	0.5473326057201581	1.4764475085482704
>10	7.714380324222294	50.57007360184069
>50	0.13934015168128505	2.7250907910358206
>100	0.05464319673775884	2.921012351175651
>500	0.0027321598368879423	0.6027095852159459
>1k	0.0013660799184439711	0.6731348900102005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	1704	0.34497209248653216	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	1584	0.3206782831564946	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	951	0.19252843894054697	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	858	0.17370073670976793	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.11782497525068174	No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	553	0.11195397132925601	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1983994428619727	0.0
2	0.0	0.0	0.0	1.0932214198516863	0.0
3	0.0	0.0	0.0	1.5145165633167528	0.0
4	0.0	0.0	0.0	2.085623530983717	0.0
5	0.0	0.0	0.0	3.6631015501474837	0.0
6	2.0244841108364562E-4	0.0	0.0	4.458521357295127	0.0
7	2.0244841108364562E-4	0.0	0.0	5.292406362548664	0.0
8	2.0244841108364562E-4	0.0	0.0	6.547991408089434	0.0
9	2.0244841108364562E-4	0.0	0.0	6.954102920723226	0.0
10	2.0244841108364562E-4	0.0	0.0	7.973025773707215	0.0
11	2.0244841108364562E-4	0.0	0.0	9.588361645743623	0.0
12	2.0244841108364562E-4	0.0	0.0	10.865608671270344	0.0
13	2.0244841108364562E-4	0.0	0.0	11.367275833935617	0.0
14	2.0244841108364562E-4	0.0	0.0	11.572760971185518	0.0
15	4.0489682216729125E-4	0.0	0.0	11.990007146428912	0.0
16	4.0489682216729125E-4	0.0	0.0	12.80243262010758	0.0
17	4.0489682216729125E-4	0.0	0.0	13.702720704196553	0.0
18	4.0489682216729125E-4	0.0	0.0	14.70322075177193	0.0
19	4.0489682216729125E-4	0.0	0.0	15.332632861830984	0.0
20	4.0489682216729125E-4	0.0	0.0	15.947873583114182	0.0
21	4.0489682216729125E-4	0.0	0.0	16.694908220012834	0.0
22	4.0489682216729125E-4	0.0	0.0	17.459960765497932	0.0
23	6.073452332509368E-4	0.0	0.0	18.21570068407318	0.0
24	6.073452332509368E-4	0.0	0.0	18.79429824295024	0.0
25	6.073452332509368E-4	0.0	0.0	19.340908952876084	0.0
26	6.073452332509368E-4	0.0	0.0	19.82941696882092	0.0
27	6.073452332509368E-4	0.0	0.0	20.356795079693818	0.0
28	6.073452332509368E-4	0.0	0.0	20.865143039924853	0.0
29	6.073452332509368E-4	0.0	0.0	21.422483515638127	0.0
30	6.073452332509368E-4	0.0	0.0	22.044809931309253	0.0
31	6.073452332509368E-4	0.0	0.0	22.596279403101104	0.0
32	6.073452332509368E-4	0.0	0.0	23.118798752107995	0.0
33	6.073452332509368E-4	0.0	0.0	23.667433946144673	0.0
34	6.073452332509368E-4	0.0	0.0	24.1919777792624	0.0
35	6.073452332509368E-4	0.0	0.0	24.796083837936	0.0
36	0.0012146904665018736	0.0	0.0	25.330142746374655	0.0
37	0.0012146904665018736	0.0	0.0	25.891937087131772	0.0
38	0.0012146904665018736	0.0	0.0	26.474583614230504	0.0
39	0.0012146904665018736	0.0	0.0	27.21574724720773	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	25	3.8875653E-5	45.0	23
CGGGTCC	25	3.8875653E-5	45.0	6
ACACGAA	20	7.029377E-4	45.0	16
GATCGGA	20	7.029377E-4	45.0	8
TTTCGCG	20	7.029377E-4	45.0	1
GTCTCGA	25	3.8875653E-5	45.0	43
TAGCCGT	20	7.029377E-4	45.0	44
CCGTCGG	20	7.029377E-4	45.0	2
CGCCTAT	25	3.8875653E-5	45.0	24
CTCGACG	25	3.8875653E-5	45.0	45
GTATACG	20	7.029377E-4	45.0	1
ATCGGTG	20	7.029377E-4	45.0	29
TATACGG	25	3.8875653E-5	45.0	2
TTACCGG	20	7.029377E-4	45.0	2
GATTACC	25	3.8875653E-5	45.0	14
GTAACGA	20	7.029377E-4	45.0	19
TTGTGCG	30	2.1629785E-6	44.999996	1
ACGGGCC	30	2.1629785E-6	44.999996	5
GCGATAT	30	2.1629785E-6	44.999996	9
CTCGATC	30	2.1629785E-6	44.999996	16
>>END_MODULE