##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935967.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	606095
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03474042848068	33.0	31.0	34.0	30.0	34.0
2	32.15806432984928	33.0	31.0	34.0	30.0	34.0
3	32.21706333165593	34.0	31.0	34.0	30.0	34.0
4	35.77522005626181	37.0	35.0	37.0	35.0	37.0
5	35.81311345581138	37.0	35.0	37.0	35.0	37.0
6	35.84427523738028	37.0	35.0	37.0	35.0	37.0
7	36.033526097393974	37.0	35.0	37.0	35.0	37.0
8	35.70504458871959	37.0	35.0	37.0	35.0	37.0
9	37.4983443189599	39.0	37.0	39.0	35.0	39.0
10	37.1096626766431	39.0	37.0	39.0	33.0	39.0
11	37.095296941898546	39.0	37.0	39.0	34.0	39.0
12	37.13385690362072	39.0	37.0	39.0	34.0	39.0
13	37.170831305323425	39.0	37.0	39.0	34.0	39.0
14	38.41655681040101	40.0	38.0	41.0	34.0	41.0
15	38.50351347561026	40.0	38.0	41.0	34.0	41.0
16	38.62116664879268	40.0	38.0	41.0	34.0	41.0
17	38.56153573284716	40.0	38.0	41.0	34.0	41.0
18	38.53464885867727	40.0	38.0	41.0	34.0	41.0
19	38.505623705854696	40.0	38.0	41.0	34.0	41.0
20	38.30110131250051	40.0	37.0	41.0	34.0	41.0
21	38.35899487704073	40.0	37.0	41.0	34.0	41.0
22	38.366414505976785	40.0	37.0	41.0	34.0	41.0
23	38.328120179179834	40.0	37.0	41.0	34.0	41.0
24	38.27395705293725	40.0	37.0	41.0	34.0	41.0
25	38.237413276796545	40.0	37.0	41.0	34.0	41.0
26	38.06463013224	40.0	37.0	41.0	34.0	41.0
27	37.993774903274236	40.0	37.0	41.0	34.0	41.0
28	38.008092790734125	40.0	37.0	41.0	34.0	41.0
29	37.97854956731206	40.0	37.0	41.0	34.0	41.0
30	37.90689413375791	40.0	37.0	41.0	33.0	41.0
31	37.9356981991272	40.0	37.0	41.0	33.0	41.0
32	37.79456520842442	40.0	37.0	41.0	33.0	41.0
33	37.71606596325658	40.0	37.0	41.0	33.0	41.0
34	37.61882873146949	40.0	37.0	41.0	33.0	41.0
35	37.59115650186852	40.0	37.0	41.0	33.0	41.0
36	37.519176036759916	40.0	37.0	41.0	33.0	41.0
37	37.44410199721166	40.0	37.0	41.0	33.0	41.0
38	37.37983154455985	40.0	37.0	41.0	33.0	41.0
39	37.33617832188023	40.0	36.0	41.0	33.0	41.0
40	37.26174444600269	40.0	36.0	41.0	32.0	41.0
41	37.21815721957779	40.0	36.0	41.0	32.0	41.0
42	37.17764706852886	40.0	36.0	41.0	32.0	41.0
43	37.11822569069205	40.0	36.0	41.0	32.0	41.0
44	37.08725034854272	40.0	36.0	41.0	32.0	41.0
45	37.024096882501915	40.0	36.0	41.0	31.0	41.0
46	36.9881536722791	40.0	35.0	41.0	31.0	41.0
47	36.94492117572328	39.0	35.0	41.0	31.0	41.0
48	36.89787904536418	39.0	35.0	41.0	31.0	41.0
49	36.87068528860987	39.0	35.0	41.0	31.0	41.0
50	36.75327465166352	39.0	35.0	41.0	31.0	41.0
51	35.83691665497983	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	3.0
10	9.0
11	12.0
12	12.0
13	9.0
14	11.0
15	13.0
16	29.0
17	50.0
18	99.0
19	212.0
20	457.0
21	881.0
22	1437.0
23	1910.0
24	2603.0
25	3717.0
26	4773.0
27	5171.0
28	5608.0
29	6340.0
30	7548.0
31	9675.0
32	12698.0
33	17372.0
34	29189.0
35	39980.0
36	40302.0
37	60180.0
38	118391.0
39	237186.0
40	212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37224362517427	21.710127950238824	24.15776404689034	10.759864377696566
2	31.695691269520452	26.84810137024724	24.617427960963216	16.83877939926909
3	30.963462823484768	26.26304457222053	27.140959750534154	15.63253285376055
4	26.531319347627026	24.43775315750831	30.76728895635173	18.26363853851294
5	26.633613542431466	28.901079863717733	25.62898555506975	18.836321038781048
6	24.083518260338728	36.20472038211832	25.28531005865417	14.426451298888788
7	77.88185020500087	5.279370395730043	12.068900089919897	4.769879309349195
8	78.3931561883863	6.96936948828154	9.488446530659385	5.149027792672766
9	72.64356247782938	6.846451463879425	10.993491119378977	9.516494938912217
10	36.786972339319746	29.061120781395655	19.32205347346538	14.829853405819222
11	27.044770209290625	24.69645847598149	28.324107606893307	19.93466370783458
12	23.699585048548492	21.686699279815873	32.73414233742235	21.879573334213283
13	22.578308680982353	23.921167473745868	35.020087610028135	18.48043623524365
14	19.495293642085812	28.758197972265076	30.96973246768246	20.776775917966656
15	18.039251272490286	26.564152484346515	36.26461198326995	19.13198425989325
16	20.432605449640732	26.292247914930826	31.356140539024413	21.91900609640403
17	20.733053399219596	27.195571651308786	29.7202583753372	22.35111657413442
18	20.92873229444229	26.779960237256535	30.963627814121548	21.327679654179626
19	21.101147509878814	28.748463524694973	29.32345589387802	20.82693307154819
20	22.491688596672137	27.702587878137912	30.990851269190472	18.81487225599947
21	21.035811217713395	29.07349507915426	31.005865417137578	18.88482828599477
22	19.81224065534281	25.13516857918313	30.68066887204151	24.371921893432546
23	19.79029690065089	27.865763617914684	30.52772255174519	21.81621692968924
24	21.155264438743103	26.052186538413945	29.960484742490863	22.83206428035209
25	18.85941972793044	30.69733292635643	27.945619086116864	22.497628259596265
26	18.828731469489107	28.887550631501664	30.103531624580306	22.180186274428927
27	21.442513137379454	28.523086314851632	28.975160659632564	21.05923988813635
28	17.687161253598855	28.910979301924616	31.898134780851183	21.50372466362534
29	19.969146750921883	27.722881726462024	30.0593141339229	22.24865738869319
30	20.324866563822503	30.206815763205437	28.910979301924616	20.557338371047443
31	20.389377902804014	28.717775266253643	27.522583093409448	23.370263737532895
32	22.143888334337024	31.102549930291456	26.360884019831875	20.39267771553964
33	20.939291695196296	30.132404986017043	26.07726511520471	22.851038203581947
34	19.185606216847194	29.218026876974733	27.80735693249408	23.789009973683996
35	20.205083361519232	30.715151915128818	26.083534759402404	22.996229963949546
36	20.593966292412905	32.47741690659055	26.741352428249698	20.187264372746846
37	20.613600178189888	29.2767635436689	29.33880002309869	20.77083625504253
38	20.60271079616232	29.60196008876496	27.08172811192965	22.713601003143072
39	23.633423803199168	26.67453122035324	26.91607751260116	22.775967463846428
40	20.164825646144582	28.67768254151577	28.448345556389675	22.709146255949975
41	19.29037527120336	29.179254077331112	26.488916753974213	25.041453897491316
42	21.124246199028203	28.822709311246587	29.059965846938184	20.993078642787022
43	23.07295061005288	26.684265667923345	28.645674357980184	21.59710936404359
44	20.92972223826298	26.802728945132365	28.59172241975268	23.675826396851978
45	20.71094465389089	25.327877642943765	27.99049653932139	25.97068116384395
46	22.614936602347814	28.317507981422054	26.635593430072845	22.431961986157287
47	19.218274362929904	27.32591425436606	31.032428909659377	22.423382473044654
48	20.370238988937377	26.854536005081712	28.772057185754708	24.003167820226203
49	20.851681667065396	24.88438281127546	31.495557627104663	22.768377894554483
50	20.099819335252725	24.492860030193285	30.275616858743266	25.13170377581072
51	19.80580602050834	24.20049662181671	27.865928608551467	28.12776874912349
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	334.0
2	503.0
3	2316.0
4	4129.0
5	2817.0
6	1505.0
7	1493.0
8	1481.0
9	1437.0
10	1393.0
11	1398.0
12	1403.0
13	1451.0
14	1499.0
15	1468.5
16	1438.0
17	1525.5
18	1613.0
19	1610.5
20	1608.0
21	1948.0
22	2288.0
23	2663.0
24	3038.0
25	3399.0
26	4700.0
27	5640.0
28	6736.5
29	7833.0
30	9227.5
31	10622.0
32	12601.0
33	14580.0
34	16070.0
35	17560.0
36	18909.0
37	20258.0
38	23298.0
39	26338.0
40	29637.0
41	32936.0
42	37489.0
43	42042.0
44	57965.0
45	73888.0
46	73079.5
47	72271.0
48	70596.0
49	68921.0
50	58163.5
51	47406.0
52	41256.0
53	35106.0
54	30769.5
55	26433.0
56	23123.0
57	19813.0
58	18916.0
59	18019.0
60	16056.0
61	14093.0
62	12523.5
63	10954.0
64	8763.5
65	6573.0
66	5216.5
67	3860.0
68	2817.5
69	1775.0
70	1587.0
71	1399.0
72	1070.0
73	741.0
74	695.0
75	494.5
76	340.0
77	241.0
78	142.0
79	98.5
80	55.0
81	33.5
82	12.0
83	8.0
84	4.0
85	3.5
86	3.0
87	4.0
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	606095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.32923115255369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12974719510329	28.030069843689287
2	11.105173909112853	8.513055555033896
3	4.01563499296229	4.617486056086047
4	2.093494744028288	3.2096817584206585
5	1.3828043276318172	2.6500913356275753
6	0.9933352648545024	2.284426618715482
7	0.7860574702873572	2.1090284934485
8	0.6486135537049482	1.9888687062909007
9	0.5881900627525455	2.029038558918964
>10	5.153191718584597	36.16714097453355
>50	0.06338279123039002	1.701920744785128
>100	0.03386203914280403	2.322086819457299
>500	0.0034730296556722087	1.0040467121895624
>1k	0.0030389009487131824	3.373057822803218
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	4752	0.7840355059850355	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC	4487	0.7403129872379742	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	3869	0.6383487737070921	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	2366	0.39036784662470403	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2276	0.37551868931438137	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	1285	0.21201296826405103	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	1236	0.20392842706176426	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	998	0.16466065550779993	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAGT	945	0.15591615175838772	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	903	0.14898654501357048	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	877	0.14469678845725506	No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	627	0.10344912926191438	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC	618	0.10196421353088213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22471724729621595	0.0
2	0.0	0.0	0.0	1.038121086628334	0.0
3	0.0	0.0	0.0	1.5482721355563072	0.0
4	0.0	0.0	0.0	2.311518821306891	0.0
5	0.0	0.0	0.0	4.698108382349302	0.0
6	0.0	0.0	0.0	5.860962390384346	0.0
7	0.0	0.0	0.0	6.978443973304515	0.0
8	0.0	0.0	0.0	8.679497438520364	0.0
9	0.0	0.0	0.0	9.29656242008266	0.0
10	0.0	0.0	0.0	11.310603123272754	0.0
11	0.0	0.0	0.0	13.171532515529744	0.0
12	0.0	0.0	0.0	15.4134252881149	0.0
13	0.0	0.0	0.0	16.11348055997822	0.0
14	0.0	0.0	0.0	16.437357179980037	0.0
15	0.0	0.0	0.0	16.95839761093558	0.0
16	0.0	0.0	0.0	17.903463978419225	0.0
17	0.0	0.0	0.0	19.092551497702505	0.0
18	0.0	0.0	0.0	20.336910880307542	0.0
19	0.0	0.0	0.0	21.318935150430214	0.0
20	0.0	0.0	0.0	22.100330806226747	0.0
21	0.0	0.0	0.0	23.056946518285088	0.0
22	0.0	0.0	0.0	24.059594618005427	0.0
23	0.0	0.0	0.0	25.102170451826858	0.0
24	0.0	0.0	0.0	25.873006706869386	0.0
25	0.0	0.0	0.0	26.562007606068356	0.0
26	0.0	0.0	0.0	27.211080771166237	0.0
27	0.0	0.0	0.0	27.875663056121564	0.0
28	0.0	0.0	0.0	28.542885191265395	0.0
29	0.0	0.0	0.0	29.246240275864345	0.0
30	0.0	0.0	0.0	29.99018305711151	0.0
31	0.0	0.0	0.0	30.699147823361024	0.0
32	0.0	0.0	0.0	31.3836939753669	0.0
33	0.0	0.0	0.0	32.032602149827994	0.0
34	0.0	0.0	0.0	32.66138146660177	0.0
35	0.0	0.0	0.0	33.31309448188815	0.0
36	0.0	0.0	0.0	33.958867834250405	0.0
37	0.0	0.0	0.0	34.62971976340343	0.0
38	0.0	0.0	0.0	35.266418630742706	0.0
39	0.0	0.0	0.0	35.88810335013488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	75	0.0	45.000004	2
TTAGGCG	20	7.030645E-4	45.0	1
CGGTTCG	20	7.030645E-4	45.0	29
CGATTAG	20	7.030645E-4	45.0	10
AAGCGCG	25	3.888616E-5	45.0	1
TGTTGCG	35	1.2106648E-7	45.0	1
AACGAGC	25	3.888616E-5	45.0	15
CGGGACG	50	2.1827873E-11	45.0	6
AGCGACT	25	3.888616E-5	45.0	19
ACGTCGC	20	7.030645E-4	45.0	11
ACGTAAG	25	3.888616E-5	45.0	1
GATTCGC	20	7.030645E-4	45.0	10
TTCGAAG	20	7.030645E-4	45.0	1
AGTACCG	20	7.030645E-4	45.0	2
TACGTCT	20	7.030645E-4	45.0	20
TGCGTAG	35	1.2106648E-7	45.0	1
CGGCGAA	120	0.0	43.124996	31
CGTTTTT	1585	0.0	41.16719	1
TTGCGCG	55	6.002665E-11	40.909092	1
CGGGAAC	140	0.0	40.17857	6
>>END_MODULE