SRR2935966.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935966.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347066
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1815	0.5229552880431964	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1424	0.41029660064656287	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	1146	0.3301965620371918	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	718	0.2068770781349945	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	453	0.13052272478433496	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	431	0.1241838728080538	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	409	0.11784502083177263	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC	354	0.10199789089106971	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	35	1.2086457E-7	45.000004	2
GCTTGCG	35	1.2086457E-7	45.000004	1
ACAATAC	35	1.2086457E-7	45.000004	30
GCGATAT	45	3.8380676E-10	45.000004	9
GCGAGAC	20	7.0264743E-4	45.0	21
TCACGAC	25	3.88516E-5	45.0	25
AAATCGT	20	7.0264743E-4	45.0	37
AATCAAT	20	7.0264743E-4	45.0	37
GACACGA	20	7.0264743E-4	45.0	25
CGTTCGG	20	7.0264743E-4	45.0	2
TCGGACC	20	7.0264743E-4	45.0	6
TACGAAA	20	7.0264743E-4	45.0	20
AATCTAA	20	7.0264743E-4	45.0	10
TTGCGGA	25	3.88516E-5	45.0	3
CTCGCCG	20	7.0264743E-4	45.0	1
TCACTCG	20	7.0264743E-4	45.0	2
CGTAAGG	50	2.1827873E-11	45.0	2
AGTTACG	20	7.0264743E-4	45.0	1
CGATGTG	40	6.7921064E-9	45.0	10
TTTGCGG	20	7.0264743E-4	45.0	2