##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935966.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347066
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.992223381143646	33.0	31.0	34.0	30.0	34.0
2	32.1050578276178	33.0	31.0	34.0	30.0	34.0
3	32.16067260982061	34.0	31.0	34.0	30.0	34.0
4	35.73894878783862	37.0	35.0	37.0	33.0	37.0
5	35.796643866008196	37.0	35.0	37.0	35.0	37.0
6	35.82076319777794	37.0	35.0	37.0	35.0	37.0
7	35.98058006258176	37.0	35.0	37.0	35.0	37.0
8	35.66505218027695	37.0	35.0	37.0	35.0	37.0
9	37.413287386260826	39.0	37.0	39.0	34.0	39.0
10	37.04031221727279	39.0	37.0	39.0	33.0	39.0
11	37.098572029527524	39.0	37.0	39.0	34.0	39.0
12	37.007937971452115	39.0	37.0	39.0	34.0	39.0
13	36.98752686808849	39.0	37.0	39.0	33.0	39.0
14	38.104910881503805	40.0	37.0	41.0	33.0	41.0
15	38.277108676735836	40.0	37.0	41.0	34.0	41.0
16	38.36770815925501	40.0	37.0	41.0	34.0	41.0
17	38.36477211827145	40.0	37.0	41.0	34.0	41.0
18	38.36321333694456	40.0	37.0	41.0	34.0	41.0
19	38.35611094143477	40.0	37.0	41.0	34.0	41.0
20	38.2262566774043	40.0	37.0	41.0	34.0	41.0
21	38.206113534601485	40.0	37.0	41.0	34.0	41.0
22	38.20116923005999	40.0	37.0	41.0	34.0	41.0
23	38.23410532866947	40.0	37.0	41.0	34.0	41.0
24	38.15118450093066	40.0	37.0	41.0	34.0	41.0
25	38.07198630807973	40.0	36.0	41.0	34.0	41.0
26	37.91832965487832	40.0	36.0	41.0	34.0	41.0
27	37.864965165127096	40.0	36.0	41.0	33.0	41.0
28	37.86656716589928	40.0	36.0	41.0	33.0	41.0
29	37.8477177251589	40.0	36.0	41.0	33.0	41.0
30	37.73413414163301	40.0	36.0	41.0	33.0	41.0
31	37.71008396097572	40.0	36.0	41.0	33.0	41.0
32	37.636195997303105	40.0	36.0	41.0	33.0	41.0
33	37.636135490079695	40.0	36.0	41.0	33.0	41.0
34	37.53613433756116	40.0	36.0	41.0	33.0	41.0
35	37.54636293961379	40.0	36.0	41.0	33.0	41.0
36	37.497308869206435	40.0	36.0	41.0	33.0	41.0
37	37.41267943273037	40.0	35.0	41.0	33.0	41.0
38	37.341491243740386	40.0	35.0	41.0	33.0	41.0
39	37.29411985040309	39.0	35.0	41.0	33.0	41.0
40	37.20796332686002	39.0	35.0	41.0	32.0	41.0
41	37.160868537972604	39.0	35.0	41.0	32.0	41.0
42	37.18250131098984	39.0	35.0	41.0	32.0	41.0
43	37.12560147061366	39.0	35.0	41.0	32.0	41.0
44	37.033670829179464	39.0	35.0	41.0	32.0	41.0
45	36.88957143598048	39.0	35.0	41.0	31.0	41.0
46	36.83691574513205	39.0	35.0	41.0	31.0	41.0
47	36.797269107316765	39.0	35.0	41.0	31.0	41.0
48	36.74525306425867	39.0	35.0	41.0	31.0	41.0
49	36.718500227622414	39.0	35.0	41.0	31.0	41.0
50	36.56143500083557	39.0	35.0	41.0	31.0	41.0
51	35.552160107875736	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	6.0
10	8.0
11	4.0
12	7.0
13	8.0
14	11.0
15	7.0
16	22.0
17	37.0
18	70.0
19	104.0
20	178.0
21	333.0
22	440.0
23	763.0
24	1093.0
25	1417.0
26	1908.0
27	2505.0
28	2986.0
29	3708.0
30	4719.0
31	6030.0
32	8014.0
33	11515.0
34	21529.0
35	29029.0
36	24778.0
37	37470.0
38	71418.0
39	116854.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2462413489077	21.351846622832547	24.67830326220373	10.723608766056024
2	32.924861553710244	24.828995061458052	24.300277180709145	17.94586620412256
3	32.62866428863674	24.499951017962	25.674367411385727	17.197017282015526
4	30.21096851895605	25.305273348584993	25.61674148432863	18.867016648130328
5	25.453371981121748	30.370304207268934	24.17868647461866	19.99763733699066
6	25.38393273901794	35.2777281554517	23.741593817890546	15.596745287639816
7	77.79586591599292	7.345288792333447	9.181538958007987	5.677306333665643
8	77.14670984769467	6.243481067001666	9.594140595736835	7.015668489566826
9	70.6430477200302	8.360657627079576	12.345778612713431	8.650516040176797
10	38.52177971913123	24.228532901523053	21.233137213094917	16.016550166250802
11	30.67773852811857	25.418796424887486	23.21345219641221	20.690012850581734
12	27.80969613848663	21.798735687160367	29.705301009029984	20.68626716532302
13	25.38191583157094	24.600508260676644	30.29596676136527	19.72160914638714
14	19.960756743674114	28.80057395423349	29.511389764482836	21.72727953760956
15	19.87575850126489	25.9135150086727	34.700892625610116	19.509833864452293
16	20.755994537062115	26.30364253484928	31.245065779995734	21.69529714809287
17	20.917635262457285	25.468642851791877	27.835339676027036	25.778382209723798
18	21.716618741103996	25.702315986008422	30.998138682556053	21.58292659033152
19	22.589075276748517	27.87049149153187	27.64690289455032	21.8935303371693
20	24.560170111736674	26.699244524096283	27.949727141235385	20.79085822293166
21	22.496009404551295	27.943100159623818	29.222107610656185	20.3387828251687
22	20.69606357292273	26.627212115274908	27.087931402096434	25.588792909705937
23	20.28807200935845	27.181285403928936	28.475275595996152	24.05536699071646
24	22.259454973981892	24.619236686970204	29.308834630877122	23.81247370817078
25	20.473915624117602	26.99400114099336	27.009848270934057	25.522234963954983
26	19.712388998058007	27.613479856857197	27.94598145597667	24.728149689108122
27	23.538174295378976	25.961056398494808	27.4503984832856	23.050370822840613
28	20.09300824627016	26.375386814035373	29.14748203511724	24.384122904577225
29	21.333118196538987	25.667164170503593	27.256775368373738	25.74294226458368
30	25.44789751805132	25.234681587940045	26.122408994254698	23.195011899753936
31	24.756962652636673	26.573331873476512	24.89007854413858	23.779626929748233
32	23.112606824062283	28.033284735468182	25.552200446024674	23.30190799444486
33	25.535200797542828	26.96749321454709	25.07592215889773	22.42138382901235
34	21.782312297949094	25.212783735658345	29.057873718543448	23.947030247849113
35	21.61462085021293	25.49832020422629	27.937337566918107	24.94972137864268
36	25.707502319443563	26.989102937193504	25.991598139835077	21.31179660352786
37	23.666391983081027	27.373756000299654	28.967112883428513	19.992739133190803
38	22.408129865789217	29.006010384192056	26.418030000057623	22.1678297499611
39	23.51310701710914	26.141137420548255	27.769069859911372	22.576685702431238
40	22.98842295125423	23.52895414704984	31.22864239078446	22.253980510911468
41	20.996582782525515	24.98919513867679	28.338414019235536	25.67580805956216
42	22.074763877763885	25.79855128419378	28.292313277589855	23.834371560452478
43	24.747166245036965	24.788945042153365	27.29624912840785	23.167639584401815
44	22.96249128407853	25.322561126702126	25.916396305025557	25.79855128419378
45	23.388346885030515	23.568139777448668	26.693770061025855	26.34974327649496
46	24.791538208870936	24.38210599713023	27.518973336483548	23.307382457515285
47	21.210086842272073	24.24293938328733	31.306149262676264	23.240824511764334
48	21.30545775155158	23.719984095244133	30.136054813781815	24.838503339422473
49	23.337059809949693	22.61817636991235	30.841684290596028	23.20307952954193
50	21.1599522857324	22.08917035952816	30.708568399094123	26.04230895564532
51	20.955668374314975	21.918309485803853	27.52358341064812	29.602438729233054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	384.5
2	581.0
3	745.0
4	909.0
5	677.0
6	445.0
7	435.0
8	425.0
9	435.0
10	445.0
11	435.5
12	426.0
13	455.0
14	484.0
15	507.5
16	531.0
17	517.5
18	504.0
19	581.0
20	658.0
21	755.5
22	853.0
23	830.5
24	808.0
25	1339.5
26	2187.5
27	2504.0
28	2881.5
29	3259.0
30	3835.5
31	4412.0
32	5090.0
33	5768.0
34	6428.0
35	7088.0
36	7563.0
37	8038.0
38	9211.0
39	10384.0
40	12504.0
41	14624.0
42	16754.5
43	18885.0
44	21753.0
45	24621.0
46	29171.0
47	33721.0
48	38603.0
49	43485.0
50	43296.5
51	43108.0
52	36609.5
53	30111.0
54	26318.5
55	22526.0
56	19843.5
57	17161.0
58	14924.0
59	12687.0
60	11965.5
61	11244.0
62	9929.5
63	8615.0
64	7183.0
65	5751.0
66	4754.0
67	3757.0
68	2946.5
69	2136.0
70	1828.5
71	1521.0
72	1302.0
73	1083.0
74	900.5
75	523.0
76	328.0
77	266.0
78	204.0
79	142.0
80	80.0
81	81.0
82	82.0
83	54.5
84	27.0
85	16.0
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	347066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.87985561320075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43077691885031	25.270461065739013
2	12.491007181853396	8.96351068296707
3	4.683657889348539	5.041469064343625
4	2.3233923547347426	3.3345192888278827
5	1.337920088377852	2.4002189796499063
6	0.9801356906039387	2.1100276236128477
7	0.758385058266814	1.9047522472875489
8	0.5635010133623467	1.6174667997866647
9	0.5432812618305936	1.7543567908655289
>10	5.735953932553803	40.404822662832686
>50	0.09782245657624787	2.344641566755603
>100	0.05093235342399684	3.3735223829478747
>500	8.084500543491561E-4	0.2082707713634199
>1k	0.002425350163047468	1.271960073020329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1815	0.5229552880431964	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1424	0.41029660064656287	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	1146	0.3301965620371918	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	718	0.2068770781349945	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	453	0.13052272478433496	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	431	0.1241838728080538	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	409	0.11784502083177263	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC	354	0.10199789089106971	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011525185411420307	0.0	0.0	0.3460436919778947	0.0
2	0.0011525185411420307	0.0	0.0	1.6699993661148023	0.0
3	0.0011525185411420307	0.0	0.0	2.356612287000167	0.0
4	0.0011525185411420307	0.0	0.0	3.1936288775045667	0.0
5	0.0011525185411420307	0.0	0.0	5.551969942316447	0.0
6	0.0011525185411420307	0.0	0.0	6.72552194683432	0.0
7	0.0011525185411420307	0.0	0.0	7.953242322785868	0.0
8	0.0011525185411420307	0.0	0.0	9.950268824949722	0.0
9	0.0011525185411420307	0.0	0.0	10.645237505258367	0.0
10	0.0011525185411420307	0.0	0.0	12.314660612102598	0.0
11	0.0011525185411420307	0.0	0.0	14.707000973878166	0.0
12	0.0011525185411420307	0.0	0.0	16.467761175107906	0.0
13	0.0011525185411420307	0.0	0.0	17.21603383794437	0.0
14	0.0011525185411420307	0.0	0.0	17.505892251041587	0.0
15	0.0011525185411420307	0.0	0.0	18.08042274380089	0.0
16	0.0011525185411420307	0.0	0.0	19.266652452271327	0.0
17	0.0011525185411420307	0.0	0.0	20.638149516230342	0.0
18	0.0011525185411420307	0.0	0.0	22.160050249808393	0.0
19	0.0011525185411420307	0.0	0.0	23.00945641463007	0.0
20	0.0011525185411420307	0.0	0.0	23.875574098298305	0.0
21	0.0017287778117130459	0.0	0.0	24.926959137455125	0.0
22	0.0020169074469985537	0.0	0.0	25.976903528435514	0.0
23	0.0020169074469985537	0.0	0.0	27.030593604674614	0.0
24	0.0020169074469985537	0.0	0.0	27.903338269954418	0.0
25	0.0020169074469985537	0.0	0.0	28.691084692824997	0.0
26	0.0020169074469985537	0.0	0.0	29.370206243192936	0.0
27	0.0020169074469985537	0.0	0.0	30.07410694219543	0.0
28	0.0020169074469985537	0.0	0.0	30.759567344539654	0.0
29	0.0020169074469985537	0.0	0.0	31.463756173177437	0.0
30	0.0020169074469985537	0.0	0.0	32.23046913267217	0.0
31	0.0020169074469985537	0.0	0.0	32.97384359170878	0.0
32	0.0020169074469985537	0.0	0.0	33.618677715477745	0.0
33	0.0020169074469985537	0.0	0.0	34.31883272922153	0.0
34	0.0020169074469985537	0.0	0.0	34.94551468596751	0.0
35	0.0020169074469985537	0.0	0.0	35.60302651368904	0.0
36	0.0020169074469985537	0.0	0.0	36.21069191450618	0.0
37	0.0023050370822840613	0.0	0.0	36.84745840848715	0.0
38	0.0023050370822840613	0.0	0.0	37.529173125572655	0.0
39	0.0023050370822840613	0.0	0.0	38.17804106423562	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	35	1.2086457E-7	45.000004	2
GCTTGCG	35	1.2086457E-7	45.000004	1
ACAATAC	35	1.2086457E-7	45.000004	30
GCGATAT	45	3.8380676E-10	45.000004	9
GCGAGAC	20	7.0264743E-4	45.0	21
TCACGAC	25	3.88516E-5	45.0	25
AAATCGT	20	7.0264743E-4	45.0	37
AATCAAT	20	7.0264743E-4	45.0	37
GACACGA	20	7.0264743E-4	45.0	25
CGTTCGG	20	7.0264743E-4	45.0	2
TCGGACC	20	7.0264743E-4	45.0	6
TACGAAA	20	7.0264743E-4	45.0	20
AATCTAA	20	7.0264743E-4	45.0	10
TTGCGGA	25	3.88516E-5	45.0	3
CTCGCCG	20	7.0264743E-4	45.0	1
TCACTCG	20	7.0264743E-4	45.0	2
CGTAAGG	50	2.1827873E-11	45.0	2
AGTTACG	20	7.0264743E-4	45.0	1
CGATGTG	40	6.7921064E-9	45.0	10
TTTGCGG	20	7.0264743E-4	45.0	2
>>END_MODULE