SRR2935964.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935964.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444985
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31470	7.0721484993876205	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23871	5.364450487095071	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	23765	5.340629459419981	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG	15658	3.5187702956279425	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	13693	3.077182376934054	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	8567	1.925233434834882	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	7960	1.788824342393564	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	7691	1.728372866501118	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	5817	1.3072350753396182	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	5034	1.1312740878906031	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	4098	0.9209299189860333	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	4016	0.9025023315392653	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	3835	0.861826803150668	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	2842	0.6386732137038327	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	2725	0.6123801925907615	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	2196	0.4934997808914907	No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	2133	0.47934200029214463	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACTGAATCTGTCTCTTATACACATCTGACGCAGCC	2025	0.4550715192646943	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCCACTTT	1539	0.3458543546411677	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1502	0.3375394676225041	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	1473	0.3310223940132813	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC	1213	0.27259345820645636	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGT	1147	0.2577614975785701	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	1078	0.24225535692214342	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	1075	0.24158117689360314	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGCCACTTTCG	937	0.21056889558074993	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA	906	0.20360236861916695	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	863	0.1939391215434228	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGT	860	0.19326494151488252	No Hit
GAATGATACGGCGACCACTGAATGATACGGCGACCACTGAATGATACGGCG	761	0.17101700057305302	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	703	0.15798285335460746	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	543	0.12202658516579212	No Hit
GAATGATACGGCGACCACTGAATGATACCTGTCTCTTATACACATCTGACG	488	0.10966661797588682	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTAT	471	0.1058462644808252	No Hit
AAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	447	0.10045282425250289	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAAGGG	60	0.0	45.000004	3
CGGGATA	30	2.162491E-6	45.000004	6
GACATAG	25	3.886938E-5	45.000004	1
TAAGGGC	30	2.162491E-6	45.000004	4
CACCGGT	25	3.886938E-5	45.000004	16
GGCTATC	30	2.162491E-6	45.000004	32
CCTCGTA	20	7.028622E-4	45.0	40
CGCCTAC	20	7.028622E-4	45.0	31
TCAAGCG	20	7.028622E-4	45.0	17
TATCCCC	20	7.028622E-4	45.0	35
ACGAGGG	65	0.0	44.999996	3
CGTTTTT	4830	0.0	44.347824	1
CGTCTGT	1570	0.0	44.28344	1
CGTTCTG	455	0.0	43.021976	1
CGTTTCT	640	0.0	42.890625	1
TGATCCG	85	0.0	42.35294	4
CGTTTTC	490	0.0	42.2449	1
ACCCGCT	70	0.0	41.785713	34
CGATGAA	720	0.0	41.562504	19
CCGATGA	705	0.0	41.489365	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content