##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935964.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444985
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.603348427475083	31.0	31.0	34.0	30.0	34.0
2	31.76366169646168	31.0	31.0	34.0	30.0	34.0
3	31.77877456543479	31.0	31.0	34.0	30.0	34.0
4	35.38372978864456	37.0	35.0	37.0	33.0	37.0
5	35.4301268582087	37.0	35.0	37.0	33.0	37.0
6	35.4652426486286	37.0	35.0	37.0	33.0	37.0
7	35.29798757261481	37.0	35.0	37.0	32.0	37.0
8	34.24392058159264	36.0	35.0	37.0	29.0	37.0
9	36.3190489567064	39.0	35.0	39.0	32.0	39.0
10	36.74202501207906	39.0	35.0	39.0	32.0	39.0
11	36.96224591840174	39.0	37.0	39.0	33.0	39.0
12	36.791950290459226	39.0	35.0	39.0	32.0	39.0
13	36.753526523365956	39.0	35.0	39.0	32.0	39.0
14	37.89991123296291	40.0	37.0	41.0	32.0	41.0
15	37.93078643100329	40.0	37.0	41.0	33.0	41.0
16	38.17227097542614	40.0	37.0	41.0	33.0	41.0
17	37.65134555097363	39.0	36.0	41.0	32.0	41.0
18	37.41483645516141	39.0	36.0	40.0	33.0	41.0
19	36.76789329977415	38.0	35.0	40.0	32.0	41.0
20	36.464492061530166	38.0	35.0	40.0	31.0	41.0
21	36.70677213838669	38.0	35.0	40.0	31.0	41.0
22	36.55921435554007	38.0	35.0	40.0	31.0	41.0
23	36.35411980179107	38.0	35.0	40.0	31.0	41.0
24	36.16538759733474	38.0	35.0	40.0	30.0	41.0
25	36.40397766216839	38.0	35.0	40.0	31.0	41.0
26	36.093457082823015	38.0	35.0	40.0	31.0	41.0
27	35.78265559513242	37.0	35.0	40.0	30.0	41.0
28	35.58389608638493	37.0	35.0	40.0	29.0	41.0
29	35.55423441239592	37.0	35.0	40.0	29.0	41.0
30	35.437327100913514	37.0	35.0	40.0	28.0	41.0
31	35.58508713776869	37.0	35.0	40.0	29.0	41.0
32	35.17698798835916	37.0	35.0	40.0	26.0	41.0
33	34.7891457015405	37.0	34.0	40.0	24.0	41.0
34	34.926536849556726	37.0	34.0	40.0	25.0	41.0
35	34.79759317729811	37.0	34.0	40.0	24.0	41.0
36	34.61070822611998	37.0	34.0	40.0	23.0	41.0
37	34.67493511017225	37.0	34.0	40.0	24.0	41.0
38	34.349596053799566	37.0	33.0	40.0	22.0	41.0
39	34.02249064575211	37.0	33.0	40.0	21.0	41.0
40	33.88565681989281	36.0	33.0	40.0	21.0	41.0
41	33.859599761789724	36.0	33.0	40.0	21.0	41.0
42	33.88890861489713	37.0	33.0	40.0	20.0	41.0
43	33.78417250019663	36.0	33.0	40.0	18.0	41.0
44	33.87844758812095	37.0	33.0	40.0	20.0	41.0
45	33.7596660561592	36.0	33.0	40.0	20.0	41.0
46	33.574118228704336	36.0	33.0	40.0	20.0	41.0
47	33.27918693888558	36.0	33.0	40.0	18.0	41.0
48	33.28896704383294	36.0	33.0	40.0	18.0	41.0
49	33.35034664089801	36.0	33.0	40.0	18.0	41.0
50	33.165881996022335	36.0	33.0	40.0	15.0	41.0
51	32.091883996089756	35.0	31.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	26.0
11	39.0
12	40.0
13	60.0
14	44.0
15	64.0
16	86.0
17	133.0
18	288.0
19	565.0
20	1182.0
21	2096.0
22	3294.0
23	4513.0
24	5743.0
25	6968.0
26	8336.0
27	9264.0
28	9746.0
29	10563.0
30	11980.0
31	13929.0
32	17507.0
33	21956.0
34	30965.0
35	53973.0
36	41515.0
37	37344.0
38	59496.0
39	93196.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.22707506994618	21.117116307291255	9.395822331089812	26.259986291672753
2	34.027888580513945	47.702506826072785	9.608638493432363	8.660966099980898
3	12.797510028427924	48.03330449340989	28.21915345461083	10.950032023551355
4	12.301538254098453	23.082800543838555	51.3334157331146	13.282245468948393
5	30.709349753362474	24.052720878231852	29.482117374742966	15.755811993662707
6	15.511758823331123	41.39308066564041	32.38401294425655	10.711147566771913
7	36.99495488611976	14.716675843005945	39.81190377203726	8.47646549883704
8	32.37794532399968	29.109969998988728	27.248783666865172	11.26330101014641
9	28.957155859186262	15.543894738024877	25.950987111925123	29.547962290863737
10	28.559839095699857	23.077407103610234	31.416115149948876	16.946638650741036
11	24.38374327224513	24.117217434295537	34.47172376596964	17.02731552748969
12	13.433935975369957	24.19295032416823	37.25496365046013	25.118150050001688
13	21.42566603368653	27.574187893973956	40.01370832724699	10.98643774509253
14	9.88280503837208	44.28711080148769	31.760621144532962	14.069463015607267
15	8.181624099688753	31.2464465094329	36.97090913176849	23.60102025910986
16	8.842320527658236	35.90233378653213	26.08290167084284	29.172444014966796
17	9.445936379877972	40.57687337775431	26.768767486544494	23.20842275582323
18	10.130229108846365	35.187028776250884	26.129869546164475	28.552872568738273
19	10.853624279470095	34.26160432374125	29.843028416688206	25.04174298010045
20	18.160836882142096	32.52986055709743	31.6630897670708	17.646212793689674
21	11.866017955661427	39.9582008382305	31.996134701169705	16.17964650493837
22	12.700203377641945	32.06737305751879	34.29216715170174	20.94025641313752
23	16.548872433902268	31.608481184759036	36.27942514916233	15.563221232176366
24	22.33805633897772	31.249592682899426	25.00870815870198	21.403642819420877
25	16.128633549445485	41.827702057372726	23.01695562771779	19.026708765464004
26	16.88259154802971	28.61175095789746	30.48687034394418	24.018787150128656
27	21.037113610571144	34.409249749991574	22.211310493612142	22.34232614582514
28	16.36998999966291	32.65413440902502	24.643077856556964	26.332797734755104
29	22.755149049967976	31.42869984381496	23.758104205759743	22.058046900457317
30	22.489072665370742	32.10759913255503	22.635594458240167	22.76773374383406
31	16.098969628189714	28.529051541063183	21.130150454509703	34.2418283762374
32	19.46177961054867	32.90133375282313	22.36749553355731	25.269391103070888
33	13.755295122307492	34.54004067552839	24.775441868827038	26.929222333337076
34	11.5253323145724	28.75422767059564	31.600166297740373	28.12027371709159
35	13.355506365383102	27.087654640044047	36.36684382619639	23.189995168376463
36	11.45184669146151	32.152095014438686	34.927694191939054	21.46836410216075
37	15.69333797768464	25.229614481387014	33.55528838050721	25.521759160421137
38	15.968852882681439	27.624751396114476	29.91269368630403	26.493702034900053
39	19.39728305448498	29.845949863478545	29.593357079452115	21.163410002584357
40	14.369248401631515	33.63394271717024	35.46411676798094	16.532692113217298
41	14.79802689978314	32.741777812735265	32.05883344382395	20.40136184365765
42	18.065103318089374	26.057282829758304	37.30058316572469	18.57703068642763
43	21.421845680191467	26.260660471701293	27.86475948627482	24.45273436183242
44	19.364023506410327	27.336427070575414	27.09080081351057	26.20874860950369
45	18.244659932357273	22.67492162657168	30.974077777902625	28.10634066316842
46	22.41626122228839	26.21369259637965	28.61377349798308	22.756272683348875
47	13.852826499769655	24.466217962403228	38.16038742878973	23.52056810903738
48	15.789071541737362	24.69049518523096	35.55737833859568	23.96305493443599
49	15.122082766834838	21.65870759688529	40.51237682169062	22.706832814589255
50	14.206546288077126	21.185208490173828	38.119936627077315	26.48830859467173
51	17.752283784846682	21.968605683337643	33.048304999044916	27.23080553277077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33715.0
1	21600.5
2	9486.0
3	19461.0
4	29436.0
5	17113.5
6	4791.0
7	4143.0
8	3495.0
9	3472.5
10	3450.0
11	3342.5
12	3235.0
13	2898.5
14	2562.0
15	2295.5
16	2029.0
17	1785.0
18	1541.0
19	1400.5
20	1260.0
21	1172.5
22	1085.0
23	975.0
24	865.0
25	942.5
26	1276.0
27	1532.0
28	1494.5
29	1457.0
30	1790.5
31	2124.0
32	2389.0
33	2654.0
34	3304.0
35	3954.0
36	4399.5
37	4845.0
38	5303.0
39	5761.0
40	6597.0
41	7433.0
42	9808.5
43	12184.0
44	16673.0
45	21162.0
46	57397.5
47	93633.0
48	73383.0
49	53133.0
50	51534.0
51	49935.0
52	35954.0
53	21973.0
54	19195.5
55	16418.0
56	14805.0
57	13192.0
58	11440.5
59	9689.0
60	8744.5
61	7800.0
62	6878.5
63	5957.0
64	5161.0
65	4365.0
66	3528.5
67	2692.0
68	2334.5
69	1977.0
70	1580.5
71	1184.0
72	992.0
73	800.0
74	659.5
75	414.0
76	309.0
77	242.0
78	175.0
79	128.5
80	82.0
81	52.5
82	23.0
83	23.5
84	24.0
85	16.0
86	8.0
87	11.5
88	15.0
89	7.5
90	0.0
91	2.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.687942275764232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42806043602063	22.499396705887083
2	9.356594045286172	5.368428597378581
3	3.7521540605593993	3.2292473729730724
4	2.0067196030621304	2.3027462454516363
5	1.3129882783698152	1.8833465969314158
6	0.8905885474769597	1.5329491704885443
7	0.6427475526285094	1.2907373281386787
8	0.5291504995409784	1.2144191188818718
9	0.37457905812930326	0.9671312157591228
>10	2.5345067949050364	12.8002237881611
>50	0.07649256435798395	1.5028344331034968
>100	0.07018389925629455	4.235898227522186
>500	0.005520081963978223	1.2607701784864953
>1k	0.011828747065667621	7.442231573958413
>5k	0.003942915688555874	7.933599388003393
>10k+	0.003942915688555874	24.5360400588749
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31470	7.0721484993876205	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23871	5.364450487095071	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	23765	5.340629459419981	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG	15658	3.5187702956279425	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	13693	3.077182376934054	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	8567	1.925233434834882	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	7960	1.788824342393564	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	7691	1.728372866501118	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	5817	1.3072350753396182	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	5034	1.1312740878906031	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	4098	0.9209299189860333	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	4016	0.9025023315392653	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	3835	0.861826803150668	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	2842	0.6386732137038327	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	2725	0.6123801925907615	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	2196	0.4934997808914907	No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	2133	0.47934200029214463	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACTGAATCTGTCTCTTATACACATCTGACGCAGCC	2025	0.4550715192646943	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCCACTTT	1539	0.3458543546411677	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1502	0.3375394676225041	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	1473	0.3310223940132813	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC	1213	0.27259345820645636	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGT	1147	0.2577614975785701	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	1078	0.24225535692214342	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	1075	0.24158117689360314	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGCCACTTTCG	937	0.21056889558074993	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA	906	0.20360236861916695	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	863	0.1939391215434228	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGT	860	0.19326494151488252	No Hit
GAATGATACGGCGACCACTGAATGATACGGCGACCACTGAATGATACGGCG	761	0.17101700057305302	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	703	0.15798285335460746	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	543	0.12202658516579212	No Hit
GAATGATACGGCGACCACTGAATGATACCTGTCTCTTATACACATCTGACG	488	0.10966661797588682	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTAT	471	0.1058462644808252	No Hit
AAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	447	0.10045282425250289	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.494533523601919E-4	0.0	0.0	0.34607908131734777	0.0
2	4.494533523601919E-4	0.0	0.0	1.927255974920503	0.0
3	4.494533523601919E-4	0.0	0.0	5.858399721338921	0.0
4	4.494533523601919E-4	0.0	0.0	11.40555299616841	0.0
5	4.494533523601919E-4	0.0	0.0	19.1125543557648	0.0
6	4.494533523601919E-4	0.0	0.0	22.5708731755003	0.0
7	4.494533523601919E-4	0.0	0.0	24.751171387799587	0.0
8	4.494533523601919E-4	0.0	0.0	26.52988302976505	0.0
9	4.494533523601919E-4	0.0	0.0	27.202265244895894	0.0
10	8.989067047203838E-4	0.0	0.0	29.95606593480679	0.0
11	8.989067047203838E-4	0.0	0.0	30.881490387316425	0.0
12	8.989067047203838E-4	0.0	0.0	33.55843455397373	0.0
13	8.989067047203838E-4	0.0	0.0	34.200253941144084	0.0
14	0.0011236333809004797	0.0	0.0	34.558917716327514	0.0
15	0.0011236333809004797	0.0	0.0	35.04432733687652	0.0
16	0.0011236333809004797	0.0	0.0	35.568165219052325	0.0
17	0.0011236333809004797	0.0	0.0	36.1081834219131	0.0
18	0.0013483600570805758	0.0	0.0	36.541905906940684	0.0
19	0.0015730867332606718	0.0	0.0	37.21541175545243	0.0
20	0.0015730867332606718	0.0	0.0	37.56418755688394	0.0
21	0.0015730867332606718	0.0	0.0	37.86891692978415	0.0
22	0.0015730867332606718	0.0	0.0	38.1776913828556	0.0
23	0.0015730867332606718	0.0	0.0	38.62040293493039	0.0
24	0.0015730867332606718	0.0	0.0	39.67077541939616	0.0
25	0.0015730867332606718	0.0	0.0	40.042473341798036	0.0
26	0.0015730867332606718	0.0	0.0	40.34562962796499	0.0
27	0.0015730867332606718	0.0	0.0	40.82025236805735	0.0
28	0.0017978134094407676	0.0	0.0	41.115992673910355	0.0
29	0.0017978134094407676	0.0	0.0	41.52679303796757	0.0
30	0.0017978134094407676	0.0	0.0	41.78904906906974	0.0
31	0.0017978134094407676	0.0	0.0	42.10636313583604	0.0
32	0.0017978134094407676	0.0	0.0	42.37670932728069	0.0
33	0.0020225400856208635	0.0	0.0	42.60211018348933	0.0
34	0.0020225400856208635	0.0	0.0	42.892906502466374	0.0
35	0.0020225400856208635	0.0	0.0	43.14190365967392	0.0
36	0.0020225400856208635	0.0	0.0	43.36550670247312	0.0
37	0.0020225400856208635	0.0	0.0	43.592705372091196	0.0
38	0.0020225400856208635	0.0	0.0	43.87069227052597	0.0
39	0.0020225400856208635	0.0	0.0	44.17991617694978	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAAGGG	60	0.0	45.000004	3
CGGGATA	30	2.162491E-6	45.000004	6
GACATAG	25	3.886938E-5	45.000004	1
TAAGGGC	30	2.162491E-6	45.000004	4
CACCGGT	25	3.886938E-5	45.000004	16
GGCTATC	30	2.162491E-6	45.000004	32
CCTCGTA	20	7.028622E-4	45.0	40
CGCCTAC	20	7.028622E-4	45.0	31
TCAAGCG	20	7.028622E-4	45.0	17
TATCCCC	20	7.028622E-4	45.0	35
ACGAGGG	65	0.0	44.999996	3
CGTTTTT	4830	0.0	44.347824	1
CGTCTGT	1570	0.0	44.28344	1
CGTTCTG	455	0.0	43.021976	1
CGTTTCT	640	0.0	42.890625	1
TGATCCG	85	0.0	42.35294	4
CGTTTTC	490	0.0	42.2449	1
ACCCGCT	70	0.0	41.785713	34
CGATGAA	720	0.0	41.562504	19
CCGATGA	705	0.0	41.489365	18
>>END_MODULE