SRR2935963.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935963.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	924898
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	53934	5.8313457267720334	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	23501	2.5409288375583037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21063	2.2773322031186143	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC	20093	2.17245577350151	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	14253	1.5410347951882262	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	10791	1.1667232494826458	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10548	1.1404500820631032	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	10271	1.1105008336054354	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	7504	0.811332709120357	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	7482	0.8089540684486289	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	5966	0.6450441021604545	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCAAAGT	5273	0.5701169210010185	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	5150	0.5568181572454476	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	4143	0.4479412864986193	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	2885	0.3119262880879838	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTA	2862	0.30943952738572256	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	2733	0.29549204344695307	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCG	1811	0.19580537529543798	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGT	1723	0.18629081260852548	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1409	0.15234112302113315	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	1200	0.12974403663971595	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	1150	0.12433803511306113	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTAT	1111	0.12012135392227034	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATG	1090	0.11785083328107532	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	1072	0.11590467273147959	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	1040	0.11244483175442048	No Hit
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT	1035	0.111904231601755	Illumina Single End PCR Primer 1 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGT	1009	0.1090931108078945	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTCAAAG	929	0.10044350836524676	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCCCCAGTCGAATGC	926	0.10011914827364747	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	40	6.8121153E-9	45.0	41
TGCGACG	25	3.890213E-5	45.0	1
ACGCTAG	20	7.0325704E-4	45.0	27
CCAACGT	45	3.8380676E-10	45.0	38
CGTTTTT	9360	0.0	44.18269	1
ATCGAAT	165	0.0	43.636368	43
TGAATGA	6095	0.0	43.52338	22
ACCCGTA	150	0.0	43.5	40
CGATGAA	5835	0.0	43.496143	19
ATGAATG	6070	0.0	43.44316	21
GATGAAT	6130	0.0	43.421696	20
CCGATGA	5610	0.0	43.315506	18
GCGAGCC	590	0.0	43.09322	33
ACCGATG	5625	0.0	42.92	17
GTCGAAT	585	0.0	42.692307	43
CAGTCGA	290	0.0	42.672417	41
CGGCTAT	90	0.0	42.5	31
CGACGTT	160	0.0	42.1875	45
CAATCGA	80	0.0	42.1875	41
CACCGAT	5880	0.0	42.130104	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content