##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935963.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	924898
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864705080992714	33.0	31.0	34.0	30.0	34.0
2	32.02437241728277	33.0	31.0	34.0	30.0	34.0
3	32.04878808257776	33.0	31.0	34.0	30.0	34.0
4	35.70620111623119	37.0	35.0	37.0	35.0	37.0
5	35.60614467757526	37.0	35.0	37.0	33.0	37.0
6	35.544902248680394	37.0	35.0	37.0	33.0	37.0
7	35.66149132120515	37.0	35.0	37.0	35.0	37.0
8	34.837719402571956	37.0	35.0	37.0	31.0	37.0
9	36.828125912262756	39.0	37.0	39.0	32.0	39.0
10	36.877620018639895	39.0	37.0	39.0	32.0	39.0
11	36.888017921976264	39.0	37.0	39.0	32.0	39.0
12	36.71511669394896	39.0	35.0	39.0	32.0	39.0
13	36.61399959779349	39.0	35.0	39.0	32.0	39.0
14	37.76241055770474	40.0	36.0	41.0	32.0	41.0
15	37.68958414873856	40.0	36.0	41.0	32.0	41.0
16	37.97919554372482	40.0	37.0	41.0	33.0	41.0
17	37.662581171112926	40.0	36.0	41.0	32.0	41.0
18	37.54108128680136	39.0	36.0	41.0	32.0	41.0
19	37.438821361923154	39.0	36.0	41.0	33.0	41.0
20	36.59072784242154	38.0	35.0	41.0	30.0	41.0
21	36.94449441992523	39.0	35.0	41.0	31.0	41.0
22	36.69218227307228	39.0	35.0	41.0	31.0	41.0
23	36.61053326961459	39.0	35.0	41.0	31.0	41.0
24	36.72746508263614	38.0	35.0	41.0	31.0	41.0
25	37.014147505995254	39.0	35.0	41.0	32.0	41.0
26	36.51233649548383	38.0	35.0	41.0	31.0	41.0
27	36.250321657090836	38.0	35.0	41.0	30.0	41.0
28	36.12662909856006	38.0	35.0	41.0	30.0	41.0
29	36.1714805308261	38.0	35.0	41.0	30.0	41.0
30	35.90578636779407	38.0	35.0	40.0	29.0	41.0
31	35.83787185181501	38.0	35.0	40.0	29.0	41.0
32	35.00200022056486	38.0	34.0	40.0	23.0	41.0
33	34.68208494342079	38.0	34.0	40.0	22.0	41.0
34	34.118626053899995	38.0	33.0	40.0	18.0	41.0
35	33.96931121053348	38.0	34.0	40.0	15.0	41.0
36	33.78792039770872	38.0	33.0	40.0	16.0	41.0
37	33.424933344001175	38.0	33.0	40.0	11.0	41.0
38	33.38017813856231	37.0	33.0	40.0	12.0	41.0
39	33.16664864666158	37.0	33.0	40.0	11.0	41.0
40	33.07931469199847	37.0	32.0	40.0	13.0	41.0
41	33.075549952535304	37.0	32.0	40.0	13.0	41.0
42	32.89994464254437	37.0	33.0	40.0	10.0	41.0
43	32.841950139366716	37.0	33.0	40.0	10.0	41.0
44	33.05329128184946	37.0	33.0	40.0	11.0	41.0
45	33.08317025228728	37.0	33.0	40.0	11.0	41.0
46	33.0341540364451	37.0	33.0	40.0	11.0	41.0
47	32.880995525993136	37.0	31.0	40.0	11.0	41.0
48	32.950710240480575	37.0	32.0	40.0	11.0	41.0
49	33.00373879065584	37.0	33.0	40.0	11.0	41.0
50	32.740031873785	37.0	32.0	40.0	10.0	41.0
51	31.887030786097494	35.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	14.0
10	34.0
11	28.0
12	38.0
13	33.0
14	43.0
15	49.0
16	101.0
17	188.0
18	476.0
19	1431.0
20	3800.0
21	6821.0
22	10247.0
23	13637.0
24	16389.0
25	20090.0
26	23927.0
27	24423.0
28	22874.0
29	20343.0
30	20712.0
31	24242.0
32	29857.0
33	38089.0
34	60911.0
35	70621.0
36	65864.0
37	80683.0
38	137701.0
39	231053.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.79457626678834	14.429807394977608	13.969972905120349	19.80564343311371
2	32.13348931449738	41.958680849131476	14.278114992139674	11.629714844231472
3	19.03766685623712	42.309962828333504	27.086121929120832	11.566248386308544
4	17.789961703885186	16.80131214469055	51.38901803225869	14.019708119165571
5	34.455475090226166	19.383867194004097	28.014440511278	18.146217204491737
6	17.912894178601316	38.491812070087725	31.55731767178651	12.037976079524444
7	52.67240279468655	5.480820587783734	36.156960010725506	5.68981660680421
8	48.98150931237823	20.395113839580148	23.00686129713763	7.616515550903992
9	44.664060253130614	6.057532830647271	21.748992861915582	27.529414054306528
10	38.08117219412303	16.20913873746078	29.35091220869761	16.358776859718585
11	31.818427545523935	17.24471238990678	32.384976505517365	18.55188355905192
12	19.993772286241292	15.99408799673045	36.39644587835632	27.61569383867194
13	26.410155498227912	17.563774600009946	42.19113891477763	13.834930986984512
14	14.761303408592083	33.865788443698655	33.6650095469987	17.707898600710564
15	12.44180439356556	21.426362690804822	42.40683837569116	23.724994539938457
16	15.879480764365367	25.65699136553436	29.485629766741845	28.977898103358424
17	15.502358097865926	31.961686585980292	32.30561640310607	20.23033891304771
18	15.365910619333158	25.93485984400442	30.99509351301441	27.70413602364801
19	14.839474190667513	26.7671678390482	28.542931220523776	29.850426749760512
20	22.97647956855783	27.382262692750984	33.931957902384916	15.709299836306274
21	16.13161667556855	34.72372088597878	34.098462749405876	15.04619968904679
22	15.5140350611635	19.5184766320178	41.34726207646681	23.620226230351886
23	23.238454402539524	24.908368274123198	35.029916812448505	16.82326051088877
24	21.494478310040673	28.601748517133778	29.074016810502346	20.829756362323195
25	17.697302837718322	37.45180549638987	26.269599458534888	18.58129220735692
26	16.955923788352877	19.97895984205826	37.832820483988506	25.232295885600358
27	28.49243916626482	20.49609794809806	29.019740555174735	21.991722330462384
28	15.746817486901257	27.387128094124975	34.022346247910576	22.843708171063188
29	20.58994613460079	19.13573172393064	40.79801232135868	19.476309820109893
30	23.009348057839894	28.640347368034096	32.50520597947017	15.845098594655843
31	19.7170931281071	19.669195954580935	32.78956165977222	27.824149257539748
32	26.832472337490188	24.891717789421104	27.828798418852674	20.447011454236037
33	24.709211177881237	28.088935212315302	25.014217784015102	22.18763582578836
34	18.16654377023196	30.771609409902496	29.67732658087703	21.384520238988518
35	25.493730119429387	30.99909395414413	25.939833365408944	17.56734256101754
36	22.594599620714934	32.831836591710655	29.08872113465485	15.484842652919564
37	21.18698494320455	25.101146288563715	30.162028677756897	23.54984009047484
38	19.352404265118963	26.984056620297594	26.48583951960108	27.177699594982364
39	22.504859995372463	28.95778777767927	25.414370017018094	23.122982209930175
40	18.625621419875486	28.75560332058238	30.837346388466617	21.781428871075516
41	20.2038495055671	29.306799236240106	26.557739339905588	23.931611918287203
42	20.118434681445954	26.077145804185974	33.48228669539776	20.32213281897031
43	25.81754961087601	22.95626112284814	27.307443631622082	23.918745634653767
44	22.96253208461906	20.258990721138982	29.455464278223115	27.323012916018847
45	19.761638580686736	19.643571507344593	30.136404230520554	30.45838568144812
46	27.650940968625733	23.81484228531146	27.315336393851	21.218880352211812
47	16.47273537190047	25.539573012375417	37.816926839500134	20.170764776223972
48	18.610917095722986	23.719588538411802	31.38486622308622	26.28462814277899
49	18.72617304827127	19.57718580859727	39.01024761649393	22.686393526637534
50	19.283531805669384	20.41857588620583	35.94244987014784	24.355442437976947
51	18.53328691380022	19.009015048145848	33.53840099124444	28.919297046809483
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11514.0
1	8056.0
2	4598.0
3	19079.5
4	33561.0
5	21713.5
6	9866.0
7	9339.5
8	8813.0
9	8654.5
10	8496.0
11	8177.0
12	7858.0
13	7458.5
14	7059.0
15	6480.0
16	5901.0
17	5441.0
18	4981.0
19	4582.5
20	4184.0
21	4074.5
22	3965.0
23	3718.0
24	3471.0
25	3876.5
26	4630.5
27	4979.0
28	5558.0
29	6137.0
30	6907.0
31	7677.0
32	8599.0
33	9521.0
34	11009.0
35	12497.0
36	13866.0
37	15235.0
38	16870.5
39	18506.0
40	20911.0
41	23316.0
42	28868.5
43	34421.0
44	112373.0
45	190325.0
46	135752.0
47	81179.0
48	83274.0
49	85369.0
50	71740.0
51	58111.0
52	55262.0
53	52413.0
54	49876.0
55	47339.0
56	44737.0
57	42135.0
58	37497.5
59	32860.0
60	28679.5
61	24499.0
62	21495.5
63	18492.0
64	16547.0
65	14602.0
66	12118.5
67	9635.0
68	7777.5
69	5920.0
70	5087.0
71	4254.0
72	3597.0
73	2940.0
74	2482.0
75	1509.0
76	994.0
77	764.5
78	535.0
79	386.5
80	238.0
81	176.0
82	114.0
83	70.0
84	26.0
85	26.5
86	27.0
87	20.5
88	14.0
89	9.0
90	4.0
91	4.5
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	924898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.2849093487928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.78806402486248	23.499560487168115
2	9.258270947270312	5.978048765183681
3	4.31023385052528	4.174665274089205
4	2.8067187111073166	3.624586366226412
5	1.9904451913046926	3.2130671282506276
6	1.5754668457902188	3.0518282579019442
7	1.325615870498817	2.995817174726275
8	1.018348216663328	2.630182388838428
9	0.847075541862197	2.461298137254052
>10	3.948528288478934	19.071686523667697
>50	0.07561977922980566	1.6573997509745741
>100	0.04272685538563576	2.558190698408167
>500	0.0033910202687005525	0.7682126131179879
>1k	0.0050865304030508285	2.8763360388269774
>5k	0.0016955101343502762	3.4348967844630005
>10k+	0.002712816214960442	18.00422361090289
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	53934	5.8313457267720334	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	23501	2.5409288375583037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21063	2.2773322031186143	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC	20093	2.17245577350151	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	14253	1.5410347951882262	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	10791	1.1667232494826458	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10548	1.1404500820631032	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	10271	1.1105008336054354	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	7504	0.811332709120357	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	7482	0.8089540684486289	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	5966	0.6450441021604545	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCAAAGT	5273	0.5701169210010185	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	5150	0.5568181572454476	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	4143	0.4479412864986193	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	2885	0.3119262880879838	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTA	2862	0.30943952738572256	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	2733	0.29549204344695307	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCG	1811	0.19580537529543798	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGT	1723	0.18629081260852548	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1409	0.15234112302113315	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	1200	0.12974403663971595	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	1150	0.12433803511306113	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTAT	1111	0.12012135392227034	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATG	1090	0.11785083328107532	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	1072	0.11590467273147959	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	1040	0.11244483175442048	No Hit
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT	1035	0.111904231601755	Illumina Single End PCR Primer 1 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGT	1009	0.1090931108078945	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTCAAAG	929	0.10044350836524676	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCCCCAGTCGAATGC	926	0.10011914827364747	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0812003053309663E-4	0.0	0.0	0.128662836334385	0.0
2	1.0812003053309663E-4	0.0	0.0	1.1568843267041338	0.0
3	1.0812003053309663E-4	0.0	0.0	2.5425506380163	0.0
4	1.0812003053309663E-4	0.0	0.0	5.200357228580882	0.0
5	1.0812003053309663E-4	0.0	0.0	12.208157007583539	0.0
6	1.0812003053309663E-4	0.0	0.0	14.812768543125836	0.0
7	2.1624006106619326E-4	0.0	0.0	16.59188364554794	0.0
8	2.1624006106619326E-4	0.0	0.0	18.4064621179849	0.0
9	2.1624006106619326E-4	0.0	0.0	19.40289631937792	0.0
10	2.1624006106619326E-4	0.0	0.0	23.16893322290674	0.0
11	2.1624006106619326E-4	0.0	0.0	24.158339622315108	0.0
12	2.1624006106619326E-4	0.0	0.0	27.179753875562493	0.0
13	2.1624006106619326E-4	0.0	0.0	27.683268857755124	0.0
14	2.1624006106619326E-4	0.0	0.0	28.10374765649834	0.0
15	2.1624006106619326E-4	0.0	0.0	28.660890173835384	0.0
16	2.1624006106619326E-4	0.0	0.0	29.095100216456302	0.0
17	2.1624006106619326E-4	0.0	0.0	29.560881307992883	0.0
18	2.1624006106619326E-4	0.0	0.0	29.8572383116841	0.0
19	2.1624006106619326E-4	0.0	0.0	30.78079961249781	0.0
20	2.1624006106619326E-4	0.0	0.0	31.125161909745724	0.0
21	2.1624006106619326E-4	0.0	0.0	31.36767513823146	0.0
22	2.1624006106619326E-4	0.0	0.0	31.811291623508755	0.0
23	2.1624006106619326E-4	0.0	0.0	32.0806186195667	0.0
24	2.1624006106619326E-4	0.0	0.0	32.3540541767849	0.0
25	2.1624006106619326E-4	0.0	0.0	32.581430600996	0.0
26	2.1624006106619326E-4	0.0	0.0	32.86275892044312	0.0
27	2.1624006106619326E-4	0.0	0.0	33.34183877573527	0.0
28	2.1624006106619326E-4	0.0	0.0	33.58446012425154	0.0
29	2.1624006106619326E-4	0.0	0.0	33.816161349683966	0.0
30	2.1624006106619326E-4	0.0	0.0	34.108734152306525	0.0
31	4.324801221323865E-4	0.0	0.0	34.340219137677884	0.0
32	4.324801221323865E-4	0.0	0.0	34.63938726216296	0.0
33	4.324801221323865E-4	0.0	0.0	34.88719837214482	0.0
34	4.324801221323865E-4	0.0	0.0	35.16993225198887	0.0
35	4.324801221323865E-4	0.0	0.0	35.425203644077506	0.0
36	4.324801221323865E-4	0.0	0.0	35.6888002785172	0.0
37	4.324801221323865E-4	0.0	0.0	35.98515728220842	0.0
38	4.324801221323865E-4	0.0	0.0	36.37038895099784	0.0
39	5.406001526654831E-4	0.0	0.0	36.95564267627349	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	40	6.8121153E-9	45.0	41
TGCGACG	25	3.890213E-5	45.0	1
ACGCTAG	20	7.0325704E-4	45.0	27
CCAACGT	45	3.8380676E-10	45.0	38
CGTTTTT	9360	0.0	44.18269	1
ATCGAAT	165	0.0	43.636368	43
TGAATGA	6095	0.0	43.52338	22
ACCCGTA	150	0.0	43.5	40
CGATGAA	5835	0.0	43.496143	19
ATGAATG	6070	0.0	43.44316	21
GATGAAT	6130	0.0	43.421696	20
CCGATGA	5610	0.0	43.315506	18
GCGAGCC	590	0.0	43.09322	33
ACCGATG	5625	0.0	42.92	17
GTCGAAT	585	0.0	42.692307	43
CAGTCGA	290	0.0	42.672417	41
CGGCTAT	90	0.0	42.5	31
CGACGTT	160	0.0	42.1875	45
CAATCGA	80	0.0	42.1875	41
CACCGAT	5880	0.0	42.130104	16
>>END_MODULE