##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935960.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	463329
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08943752711356	33.0	31.0	34.0	30.0	34.0
2	32.204919182697395	33.0	31.0	34.0	30.0	34.0
3	32.25575994595633	34.0	31.0	34.0	30.0	34.0
4	35.81878535554649	37.0	35.0	37.0	35.0	37.0
5	35.85293819294713	37.0	35.0	37.0	35.0	37.0
6	35.87153620861202	37.0	35.0	37.0	35.0	37.0
7	36.0460946757056	37.0	35.0	37.0	35.0	37.0
8	35.76433592544391	37.0	35.0	37.0	35.0	37.0
9	37.50735870191592	39.0	37.0	39.0	35.0	39.0
10	37.19889538535252	39.0	37.0	39.0	34.0	39.0
11	37.128489690910776	39.0	37.0	39.0	34.0	39.0
12	36.988019312410835	39.0	35.0	39.0	33.0	39.0
13	36.957246362735766	39.0	35.0	39.0	33.0	39.0
14	38.09011523129353	40.0	37.0	41.0	33.0	41.0
15	38.202521318544704	40.0	37.0	41.0	34.0	41.0
16	38.28890701855485	40.0	37.0	41.0	34.0	41.0
17	38.21071635921775	40.0	37.0	41.0	34.0	41.0
18	38.2011508021298	40.0	37.0	41.0	34.0	41.0
19	38.23886482391562	40.0	37.0	41.0	34.0	41.0
20	38.110921181277234	40.0	36.0	41.0	34.0	41.0
21	38.09314115887415	40.0	36.0	41.0	34.0	41.0
22	38.07556185777277	40.0	36.0	41.0	34.0	41.0
23	38.09903114201788	40.0	36.0	41.0	34.0	41.0
24	37.9847279147215	40.0	36.0	41.0	34.0	41.0
25	37.91129413440557	40.0	36.0	41.0	34.0	41.0
26	37.78224112887387	40.0	36.0	41.0	33.0	41.0
27	37.72431684612878	40.0	35.0	41.0	33.0	41.0
28	37.721098830420715	40.0	36.0	41.0	33.0	41.0
29	37.71295343049971	40.0	36.0	41.0	33.0	41.0
30	37.58087665568095	40.0	35.0	41.0	33.0	41.0
31	37.62838069708565	40.0	35.0	41.0	33.0	41.0
32	37.57050389679903	40.0	35.0	41.0	33.0	41.0
33	37.493504615510794	40.0	35.0	41.0	33.0	41.0
34	37.43791776469852	40.0	35.0	41.0	33.0	41.0
35	37.41215853097906	40.0	35.0	41.0	33.0	41.0
36	37.30274599690501	40.0	35.0	41.0	33.0	41.0
37	37.30475105162854	40.0	35.0	41.0	33.0	41.0
38	37.22209272460822	39.0	35.0	41.0	33.0	41.0
39	37.14534812196085	39.0	35.0	41.0	32.0	41.0
40	37.103380103554926	39.0	35.0	41.0	32.0	41.0
41	36.99765393489292	39.0	35.0	41.0	32.0	41.0
42	36.96367807756475	39.0	35.0	41.0	32.0	41.0
43	36.89879329806681	39.0	35.0	41.0	32.0	41.0
44	36.859156236713005	39.0	35.0	41.0	32.0	41.0
45	36.77196980978959	39.0	35.0	41.0	32.0	41.0
46	36.7046785329647	39.0	35.0	41.0	31.0	41.0
47	36.658687455350304	39.0	35.0	41.0	31.0	41.0
48	36.59705306596393	39.0	35.0	41.0	31.0	41.0
49	36.5624577783821	39.0	35.0	41.0	31.0	41.0
50	36.44781354070218	38.0	35.0	40.0	31.0	41.0
51	35.538345322654095	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	8.0
9	7.0
10	15.0
11	9.0
12	11.0
13	10.0
14	13.0
15	18.0
16	45.0
17	50.0
18	87.0
19	200.0
20	276.0
21	528.0
22	754.0
23	1107.0
24	1655.0
25	2214.0
26	2731.0
27	3505.0
28	4140.0
29	4984.0
30	6351.0
31	8158.0
32	10617.0
33	15676.0
34	32298.0
35	40696.0
36	32177.0
37	48200.0
38	88577.0
39	158083.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83241497942067	21.57905073932346	24.451523647343464	11.137010633912404
2	32.11842988459605	24.716562097343356	25.08239285691161	18.08261516114899
3	32.30706474233212	24.21799628341848	26.097438321365594	17.377500652883803
4	29.42790112425512	25.659088897953726	25.701175622505822	19.211834355285337
5	26.060315672017076	29.408260652797473	23.6840344549985	20.84738922018695
6	26.208158781341123	34.26873776517335	23.549572765788458	15.97353068769708
7	79.318583555098	5.9212784004454715	9.334619676299132	5.4255183681574
8	79.41937586466636	5.7792626837517185	8.917421529841645	5.883939921740276
9	73.73529392720938	7.511509100444824	10.928951134075355	7.82424583827043
10	42.22118624131017	24.530517191887405	17.902829307036686	15.345467259765739
11	31.913823654465833	24.51346667270989	23.91669850149678	19.6560111713275
12	30.208124248644054	20.906742293273247	28.2926387081318	20.592494749950898
13	26.052977473890042	22.740428507604747	29.673083273440685	21.533510745064522
14	20.710553408053457	26.289958107521873	30.10538947486559	22.89409900955908
15	20.527098454877635	25.095558447668935	33.317793619652555	21.05954947780087
16	23.736265159314442	24.511955867213146	30.31107485177919	21.440704121693223
17	22.4395623843964	25.12037882368684	28.45019413850633	23.98986465341043
18	23.590796172913848	26.019092264891686	28.7661683166821	21.62394324551237
19	24.735339251374295	27.751122852228114	26.098949126862337	21.414588769535257
20	26.57269456476931	26.839459649622622	26.924712245510207	19.663133540097856
21	24.849081322343302	27.56896287519236	27.810907584027767	19.771048218436576
22	22.62301733757222	25.415417554264895	27.700402953408915	24.261162154753965
23	23.63353038553598	25.03685286265267	27.982276093229647	23.3473406585817
24	24.5477835404216	24.038857917376205	28.270192455037353	23.143166087164843
25	22.63229799990935	25.544267680201326	25.98995530174023	25.83347901814909
26	20.773791409559948	26.59902574628396	27.884937053368127	24.74224579078797
27	21.75257754209212	26.02297719331188	27.8383179123258	24.3861273522702
28	19.009170589365223	26.882409691601435	28.799406037610424	25.309013681422922
29	21.716749868883667	24.614690640991608	27.81198673081115	25.85657275931358
30	22.675032212531484	24.650518314200063	29.186388074133067	23.488061399135386
31	24.556848373402055	25.19613492788062	24.930880648524052	25.31613605019328
32	25.08023456334484	25.285488281545078	24.677065325071386	24.9572118300387
33	23.645185170796562	25.978948004549686	25.930386399297262	24.445480425356497
34	22.630787194412612	24.403177871447717	27.139678284760937	25.826356649378734
35	22.064882621204372	24.764044555812394	27.173347664402613	25.99772515858062
36	21.768980573199606	28.128608397056954	27.327449825070303	22.774961204673136
37	23.44079477002303	26.338088054060936	27.027231189931992	23.193885985984043
38	22.134595503411184	27.038022657765865	24.88318236069834	25.944199478124613
39	24.115477338996698	24.64296428671635	26.48701031016837	24.754548064118588
40	24.99239201517712	22.87510602617147	28.176954172952694	23.955547785698716
41	21.582935667743655	24.53181216802747	27.312125940746206	26.573126223482667
42	22.61222586973835	23.551731059355234	28.05824802678011	25.77779504412631
43	21.95891040707575	23.06093510227074	28.327818893270223	26.652335597383285
44	21.35760981937241	23.436694012246157	26.798883730567262	28.406812437814168
45	23.168633951252783	22.774961204673136	26.3480162044681	27.708388639605978
46	23.568997407889427	24.436199763019367	27.030036971568798	24.96476585752241
47	21.307753237979924	24.100153454672597	29.46394462682025	25.12814868052723
48	21.355451525805638	22.62560728985235	29.923876985899867	26.09506419844214
49	22.363158792132587	22.079774846815113	30.507695395712336	25.04937096533996
50	20.91731793175044	22.274021267824807	29.25523763891317	27.55342316151158
51	20.130619926661183	21.914017900886844	28.12947171448366	29.825890457968313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	322.0
1	527.5
2	733.0
3	1111.5
4	1490.0
5	1054.5
6	619.0
7	647.0
8	675.0
9	655.0
10	635.0
11	637.5
12	640.0
13	663.0
14	686.0
15	739.0
16	792.0
17	774.0
18	756.0
19	825.0
20	894.0
21	1089.0
22	1284.0
23	1485.5
24	1687.0
25	2191.0
26	2926.0
27	3157.0
28	4301.0
29	5445.0
30	5985.0
31	6525.0
32	7325.0
33	8125.0
34	9589.5
35	11054.0
36	11493.5
37	11933.0
38	13112.5
39	14292.0
40	15499.0
41	16706.0
42	18218.5
43	19731.0
44	22603.5
45	25476.0
46	27550.5
47	29625.0
48	34503.5
49	39382.0
50	43168.5
51	46955.0
52	47247.5
53	47540.0
54	40989.5
55	34439.0
56	31456.5
57	28474.0
58	26269.0
59	24064.0
60	23816.5
61	23569.0
62	20849.5
63	18130.0
64	15436.5
65	12743.0
66	10250.5
67	7758.0
68	6461.5
69	5165.0
70	4108.0
71	3051.0
72	2907.0
73	2763.0
74	2078.5
75	1147.5
76	901.0
77	680.0
78	459.0
79	382.5
80	306.0
81	232.5
82	159.0
83	107.5
84	56.0
85	41.5
86	27.0
87	21.0
88	15.0
89	7.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	463329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.06617644950241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.76186901989844	22.69062577888641
2	11.359531338358632	7.285134725589202
3	4.022639412273992	3.8697199556010133
4	2.029302287575372	2.6028786089108302
5	1.252261196590308	2.007761424536492
6	0.9698471402247784	1.8659573716495828
7	0.7259829865770462	1.6295648982841409
8	0.6153333525404504	1.578511028626076
9	0.5557484693081266	1.6038655630537733
>10	7.55566627127249	48.506706195428954
>50	0.10007768264984149	2.1972570317043667
>100	0.04561363767033592	2.398754124626685
>500	0.004765603935706738	1.076252078122457
>1k	0.0013616011244876392	0.6870112149799943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1651	0.3563342678744477	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1496	0.3228807175894451	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	940	0.20287959527679036	Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	758	0.1635986523614969	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC	705	0.15215969645759275	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	684	0.1476272799673666	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	665	0.1435265221904953	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	637	0.1374833002035271	No Hit
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	541	0.11676368196249318	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.474880700323097E-4	0.0	0.0	0.21237608697059757	0.0
2	6.474880700323097E-4	0.0	0.0	1.143032272963704	0.0
3	6.474880700323097E-4	0.0	0.0	1.5725326927518026	0.0
4	6.474880700323097E-4	0.0	0.0	2.1917471170593683	0.0
5	6.474880700323097E-4	0.0	0.0	3.9162236769120864	0.0
6	6.474880700323097E-4	0.0	0.0	4.827671050160901	0.0
7	6.474880700323097E-4	0.0	0.0	5.740197570193103	0.0
8	6.474880700323097E-4	0.0	0.0	7.159275590347248	0.0
9	6.474880700323097E-4	0.0	0.0	7.677266046373096	0.0
10	6.474880700323097E-4	0.0	0.0	8.964688158954004	0.0
11	6.474880700323097E-4	0.0	0.0	10.709884337047757	0.0
12	6.474880700323097E-4	0.0	0.0	12.134142261762161	0.0
13	6.474880700323097E-4	0.0	0.0	12.69832020011698	0.0
14	6.474880700323097E-4	0.0	0.0	12.908537993520802	0.0
15	6.474880700323097E-4	0.0	0.0	13.345160782079256	0.0
16	6.474880700323097E-4	0.0	0.0	14.187974419904647	0.0
17	6.474880700323097E-4	0.0	0.0	15.174314579920532	0.0
18	6.474880700323097E-4	0.0	0.0	16.202093976418485	0.0
19	6.474880700323097E-4	0.0	0.0	16.884762231589217	0.0
20	6.474880700323097E-4	0.0	0.0	17.53850935296517	0.0
21	6.474880700323097E-4	0.0	0.0	18.355639297345945	0.0
22	6.474880700323097E-4	0.0	0.0	19.17600668207688	0.0
23	6.474880700323097E-4	0.0	0.0	19.937452652434878	0.0
24	6.474880700323097E-4	0.0	0.0	20.597027166441126	0.0
25	6.474880700323097E-4	0.0	0.0	21.140053827841555	0.0
26	6.474880700323097E-4	0.0	0.0	21.653727696733853	0.0
27	6.474880700323097E-4	0.0	0.0	22.159847538142444	0.0
28	6.474880700323097E-4	0.0	0.0	22.691866902352324	0.0
29	6.474880700323097E-4	0.0	0.0	23.205540771244625	0.0
30	6.474880700323097E-4	0.0	0.0	23.815906191928413	2.1582935667743654E-4
31	6.474880700323097E-4	0.0	0.0	24.37857332478649	2.1582935667743654E-4
32	6.474880700323097E-4	0.0	0.0	24.95462187775857	2.1582935667743654E-4
33	6.474880700323097E-4	0.0	0.0	25.52527469681371	2.1582935667743654E-4
34	6.474880700323097E-4	0.0	0.0	26.053840791316752	2.1582935667743654E-4
35	8.633174267097462E-4	0.0	0.0	26.630536832358864	2.1582935667743654E-4
36	0.0010791467833871828	0.0	0.0	27.1763692753961	2.1582935667743654E-4
37	0.0010791467833871828	0.0	0.0	27.709899445102725	2.1582935667743654E-4
38	0.0010791467833871828	0.0	0.0	28.280768093514542	2.1582935667743654E-4
39	0.0010791467833871828	0.0	0.0	28.863723185900298	2.1582935667743654E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGAC	20	7.0289226E-4	45.0	24
ATTACCC	20	7.0289226E-4	45.0	18
GCACGAG	20	7.0289226E-4	45.0	1
ATATGCG	20	7.0289226E-4	45.0	1
CTGCGAC	20	7.0289226E-4	45.0	32
TGTTACG	25	3.8871884E-5	45.0	1
ATGCGAG	40	6.7993824E-9	45.0	1
TATCGTG	25	3.8871884E-5	45.0	1
TTCGACG	20	7.0289226E-4	45.0	25
ATCGCGG	35	1.209828E-7	45.0	2
TTTAGCG	65	0.0	44.999996	1
TATGCGG	65	0.0	41.53846	2
CGATGAA	110	0.0	40.909092	19
TACACGG	45	1.9239451E-8	40.0	2
TAAGGGA	260	0.0	38.942307	4
GTTTGCG	35	6.2402196E-6	38.571426	1
TTAGACG	35	6.2402196E-6	38.571426	1
CGTAAGG	35	6.2402196E-6	38.571426	2
TTTGCGC	70	0.0	38.571426	13
GCGTAAG	35	6.2402196E-6	38.571426	1
>>END_MODULE