SRR2935959.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935959.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351347
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2017	0.5740763404839091	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1793	0.5103217047534204	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	1263	0.35947368271253205	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	730	0.2077718039431104	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	583	0.16593282424497718	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	532	0.15141725985991056	No Hit
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	452	0.12864774709902177	No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	422	0.12010917981368846	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	411	0.11697837180906624	No Hit
TAGGTTGGGCATGGATAGAAGCATCTAGAAGGGAATGGTGGGCCTGAGGTA	387	0.11014751798079962	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	373	0.10616285324764407	No Hit
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	372	0.10587823433813295	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	20	7.0265913E-4	45.000004	12
AGATCGG	20	7.0265913E-4	45.000004	1
GTCGAGG	20	7.0265913E-4	45.000004	1
AGGCGTA	20	7.0265913E-4	45.000004	22
TCTAGTT	20	7.0265913E-4	45.000004	12
ATCGTAG	20	7.0265913E-4	45.000004	38
CCGTACG	20	7.0265913E-4	45.000004	1
CGTAGTC	20	7.0265913E-4	45.000004	40
GTAGACG	20	7.0265913E-4	45.000004	1
TCGTAGT	20	7.0265913E-4	45.000004	39
ACGTCAT	20	7.0265913E-4	45.000004	25
TTACGCG	20	7.0265913E-4	45.000004	1
CGACTGG	20	7.0265913E-4	45.000004	2
TACGTCA	20	7.0265913E-4	45.000004	24
GTACGCG	20	7.0265913E-4	45.000004	11
CGAAGTA	20	7.0265913E-4	45.000004	16
TAGGTTG	95	0.0	45.0	1
TACGGGT	25	3.885258E-5	45.0	4
CACACGG	25	3.885258E-5	45.0	2
TACGGTT	60	0.0	44.999996	33