##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935959.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351347
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06088852331171	33.0	31.0	34.0	30.0	34.0
2	32.17272383142591	33.0	31.0	34.0	30.0	34.0
3	32.23055270146038	34.0	31.0	34.0	30.0	34.0
4	35.80249724631205	37.0	35.0	37.0	35.0	37.0
5	35.82602384537224	37.0	35.0	37.0	35.0	37.0
6	35.85672284095211	37.0	35.0	37.0	35.0	37.0
7	36.026432558126295	37.0	35.0	37.0	35.0	37.0
8	35.70247931532075	37.0	35.0	37.0	35.0	37.0
9	37.40639880232363	39.0	37.0	39.0	34.0	39.0
10	37.18986358215667	39.0	37.0	39.0	34.0	39.0
11	37.184376129581295	39.0	37.0	39.0	34.0	39.0
12	37.106959786194274	39.0	37.0	39.0	34.0	39.0
13	37.05674447198923	39.0	37.0	39.0	33.0	39.0
14	38.20365052213339	40.0	37.0	41.0	33.0	41.0
15	38.343108095415644	40.0	37.0	41.0	34.0	41.0
16	38.4373254930311	40.0	38.0	41.0	34.0	41.0
17	38.43154203678984	40.0	38.0	41.0	34.0	41.0
18	38.42180237770637	40.0	37.0	41.0	34.0	41.0
19	38.435575086737614	40.0	37.0	41.0	34.0	41.0
20	38.31096038958636	40.0	37.0	41.0	34.0	41.0
21	38.296362285717535	40.0	37.0	41.0	34.0	41.0
22	38.287778179406686	40.0	37.0	41.0	34.0	41.0
23	38.330234782138454	40.0	37.0	41.0	34.0	41.0
24	38.240992523061244	40.0	37.0	41.0	34.0	41.0
25	38.1705778048482	40.0	37.0	41.0	34.0	41.0
26	38.02278658989546	40.0	37.0	41.0	34.0	41.0
27	37.98780977210564	40.0	36.0	41.0	34.0	41.0
28	38.00912203604983	40.0	36.0	41.0	34.0	41.0
29	37.962649460504856	40.0	36.0	41.0	34.0	41.0
30	37.86119420402053	40.0	36.0	41.0	34.0	41.0
31	37.904242814084085	40.0	36.0	41.0	34.0	41.0
32	37.833583323608856	40.0	36.0	41.0	34.0	41.0
33	37.82310934773884	40.0	36.0	41.0	34.0	41.0
34	37.78646750932838	40.0	36.0	41.0	33.0	41.0
35	37.799861675209975	40.0	36.0	41.0	33.0	41.0
36	37.76757735230413	40.0	36.0	41.0	33.0	41.0
37	37.73018981235075	40.0	36.0	41.0	33.0	41.0
38	37.66277782363305	40.0	36.0	41.0	33.0	41.0
39	37.61199042541988	40.0	36.0	41.0	33.0	41.0
40	37.536856725687144	40.0	36.0	41.0	33.0	41.0
41	37.484617771035474	40.0	36.0	41.0	33.0	41.0
42	37.48935952206793	40.0	35.0	41.0	33.0	41.0
43	37.43904459124455	40.0	35.0	41.0	33.0	41.0
44	37.33801626312449	39.0	35.0	41.0	33.0	41.0
45	37.23827441247541	39.0	35.0	41.0	33.0	41.0
46	37.20810765425633	39.0	35.0	41.0	33.0	41.0
47	37.199426777516244	39.0	35.0	41.0	33.0	41.0
48	37.1646975781777	39.0	35.0	41.0	33.0	41.0
49	37.14709389862443	39.0	35.0	41.0	33.0	41.0
50	37.04050696320162	39.0	35.0	41.0	32.0	41.0
51	36.142693690283394	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	6.0
9	13.0
10	8.0
11	13.0
12	11.0
13	11.0
14	9.0
15	17.0
16	21.0
17	19.0
18	59.0
19	100.0
20	197.0
21	268.0
22	403.0
23	553.0
24	959.0
25	1355.0
26	1815.0
27	2258.0
28	2718.0
29	3383.0
30	4308.0
31	5566.0
32	7628.0
33	10654.0
34	20771.0
35	28053.0
36	23609.0
37	35425.0
38	68072.0
39	132934.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.692267188847495	22.09382746970943	23.94982737863138	11.264077962811694
2	33.31720492846104	24.674467122246668	22.88022951668863	19.128098432603664
3	32.75109791744344	24.46897226957965	24.78262230786089	17.997307505116027
4	30.65943355144629	25.192473537556886	24.684144165170046	19.463948745826777
5	26.170708729546572	28.922404346699988	24.704636726654847	20.202250197098596
6	27.078073813067995	33.385798085653214	23.91111920693787	15.625008894340922
7	77.724870284932	6.652682390912687	9.66537354808779	5.957073776067534
8	75.90103231278479	6.307724272585221	10.294665957016852	7.49657745761313
9	68.51687932442856	9.229052759807256	13.45792051732333	8.796147398440858
10	38.55874676601764	26.110654139639728	19.98764753932722	15.342951555015413
11	29.229508150062472	25.110503291617686	26.05486883337555	19.605119724944288
12	28.32185844763154	22.192020993490765	30.237343708641312	19.248776850236375
13	24.82076124173538	24.80112253697911	30.770434926155627	19.607681295129886
14	19.158837274830866	29.219831107139093	28.954850902384255	22.666480715645786
15	17.989907413468735	27.93022282814426	34.70671444469428	19.37315531369273
16	20.39607567447566	26.996957423857324	31.064161640771086	21.54280526089592
17	20.01696328700686	26.63947607351137	28.000239079883986	25.343321559597776
18	21.199270237116014	27.34561558800844	29.737268284630293	21.717845890245254
19	22.292491468548185	28.75647152245501	27.391154613530215	21.55988239546659
20	24.388140499278492	27.33394621271848	28.590538698210032	19.687374589793
21	22.97557685137485	27.425308882671544	29.201615496930383	20.39749876902322
22	19.59857349002553	26.245278883838484	28.391305461552253	25.76484216458373
23	19.80179139141646	26.001645097296972	30.155942700521138	24.040620810765425
24	23.022538971444185	24.662513128047202	29.37437917500363	22.940568725504985
25	20.666179019601707	27.534602543923814	26.840132404716705	24.959086031757778
26	19.063489940144642	29.306924493449493	27.848252582205056	23.781332984200805
27	21.333041124586234	27.73127421039599	29.31062453927314	21.625060125744632
28	19.1673758421162	27.6752042852223	29.37836383973678	23.779056032924714
29	19.522011003367044	27.55907977014177	29.921701337993493	22.997207888497694
30	22.527586687804366	26.250402024209684	28.856088140783896	22.365923147202054
31	23.635323483621605	26.926371934298572	26.262071399499643	23.176233182580184
32	23.109063119935563	26.343472407619817	29.086344838578388	21.461119633866236
33	24.568873506818043	24.577412074103382	28.58427708220079	22.269437336877786
34	20.608401380970946	25.19475048883298	29.768860983586027	24.427987146610047
35	19.6387047562666	25.48933106017698	31.081808013160778	23.790156170395647
36	21.92447921855032	26.085323056693237	31.6203069899558	20.369890734800638
37	21.787292904165966	25.516369856580535	31.83348655317962	20.86285068607388
38	21.402488138506946	25.67518720808773	30.09958815643794	22.822736496967387
39	23.09027827190783	23.10137840937876	30.3036599145574	23.504683404156005
40	22.307007032933253	23.864157086868538	30.444261655855893	23.384574224342316
41	18.665308085738598	25.19418125101395	30.331837186599003	25.80867347664844
42	20.04969446160064	24.702075156469245	31.988603858863172	23.259626523066938
43	23.47963694011903	22.033488260893076	32.21146046501037	22.27541433397752
44	21.848201350801343	23.73465548304098	28.75163300099332	25.665510165164353
45	22.090981280614322	22.92946858803405	28.15706409902461	26.822486032327014
46	23.201279646617163	24.324955101367024	28.937773767813585	23.535991484202228
47	19.22942276438962	23.271580517266408	33.96300523414175	23.535991484202228
48	20.073317831090062	21.985103046276187	33.13106416164077	24.81051496099298
49	21.458273444771123	20.324920947097883	34.51915058332646	23.697655024804536
50	19.966585740023397	20.69378705382428	32.96570057521482	26.373926630937504
51	18.998881447685623	20.661055879230506	29.636797809572872	30.703264863511002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	354.0
2	528.0
3	819.5
4	1111.0
5	825.0
6	539.0
7	503.0
8	467.0
9	488.5
10	510.0
11	537.5
12	565.0
13	553.5
14	542.0
15	554.5
16	567.0
17	594.0
18	621.0
19	652.0
20	683.0
21	1029.0
22	1375.0
23	1356.5
24	1338.0
25	1819.0
26	2536.0
27	2772.0
28	3521.0
29	4270.0
30	4758.0
31	5246.0
32	6083.5
33	6921.0
34	7362.5
35	7804.0
36	8640.5
37	9477.0
38	10109.5
39	10742.0
40	13836.0
41	16930.0
42	20212.5
43	23495.0
44	29088.5
45	34682.0
46	39651.5
47	44621.0
48	43773.0
49	42925.0
50	36513.5
51	30102.0
52	25280.5
53	20459.0
54	18631.5
55	16804.0
56	15041.0
57	13278.0
58	12793.0
59	12308.0
60	12026.5
61	11745.0
62	10428.0
63	9111.0
64	7661.0
65	6211.0
66	5105.0
67	3999.0
68	3206.5
69	2414.0
70	1967.5
71	1521.0
72	1234.0
73	947.0
74	801.5
75	513.5
76	371.0
77	261.0
78	151.0
79	93.5
80	36.0
81	22.5
82	9.0
83	6.5
84	4.0
85	3.5
86	3.0
87	3.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	351347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.654237176225024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.7842733741246	19.211282079256904
2	14.519001609754545	8.610998345953085
3	5.79745486164822	5.157573044573255
4	2.9022923201489146	3.4426105926572923
5	1.7310492315025017	2.5666472237348623
6	1.1410025074163643	2.030133538415539
7	0.8061676123670912	1.673439990664733
8	0.6327758632115328	1.5011588421652253
9	0.5221645325862451	1.3935951804892672
>10	6.917683288688803	44.7859393646378
>50	0.15645005413981358	3.017683083021158
>100	0.08370576145060091	4.564644592971781
>500	0.0029894914803786036	0.5452041997820247
>1k	0.0029894914803786036	1.4990899216770792
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2017	0.5740763404839091	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1793	0.5103217047534204	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	1263	0.35947368271253205	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	730	0.2077718039431104	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	583	0.16593282424497718	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	532	0.15141725985991056	No Hit
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	452	0.12864774709902177	No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	422	0.12010917981368846	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	411	0.11697837180906624	No Hit
TAGGTTGGGCATGGATAGAAGCATCTAGAAGGGAATGGTGGGCCTGAGGTA	387	0.11014751798079962	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	373	0.10616285324764407	No Hit
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	372	0.10587823433813295	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.846189095111101E-4	0.0	0.0	0.3739892470975987	0.0
2	2.846189095111101E-4	0.0	0.0	1.8836079431445267	0.0
3	2.846189095111101E-4	0.0	0.0	2.660048328290835	0.0
4	2.846189095111101E-4	0.0	0.0	3.632306523180787	0.0
5	2.846189095111101E-4	0.0	0.0	6.180784238943267	0.0
6	2.846189095111101E-4	0.0	0.0	7.447338386267707	0.0
7	2.846189095111101E-4	0.0	0.0	8.691123020831258	0.0
8	2.846189095111101E-4	0.0	0.0	10.578431009799429	0.0
9	2.846189095111101E-4	0.0	0.0	11.24016997441276	0.0
10	2.846189095111101E-4	0.0	0.0	12.717342114775422	0.0
11	2.846189095111101E-4	0.0	0.0	15.103871671026079	0.0
12	2.846189095111101E-4	0.0	0.0	16.837200829948742	0.0
13	2.846189095111101E-4	0.0	0.0	17.569525284120825	0.0
14	5.692378190222202E-4	0.0	0.0	17.86780590128847	0.0
15	5.692378190222202E-4	0.0	0.0	18.4911213131178	0.0
16	5.692378190222202E-4	0.0	0.0	19.69335158689273	0.0
17	5.692378190222202E-4	0.0	0.0	21.068345538740903	0.0
18	5.692378190222202E-4	0.0	0.0	22.56515638385983	0.0
19	8.538567285333303E-4	0.0	0.0	23.45487509499156	0.0
20	0.0011384756380444403	0.0	0.0	24.362809416332002	0.0
21	0.0011384756380444403	0.0	0.0	25.437815037555467	0.0
22	0.0011384756380444403	0.0	0.0	26.535305552630305	0.0
23	0.0011384756380444403	0.0	0.0	27.512117650072437	0.0
24	0.0011384756380444403	0.0	0.0	28.335520155288076	0.0
25	0.0011384756380444403	0.0	0.0	29.03340572140932	0.0
26	0.0011384756380444403	0.0	0.0	29.705675585674562	0.0
27	0.0011384756380444403	0.0	0.0	30.348060464441136	0.0
28	0.0011384756380444403	0.0	0.0	31.022322661072955	0.0
29	0.0011384756380444403	0.0	0.0	31.72504674865589	0.0
30	0.0011384756380444403	0.0	0.0	32.47985609667935	0.0
31	0.0011384756380444403	0.0	0.0	33.19652651082833	0.0
32	0.0011384756380444403	0.0	0.0	33.87050408855063	0.0
33	0.0011384756380444403	0.0	0.0	34.48869636000877	0.0
34	0.0011384756380444403	0.0	0.0	35.11286562856663	0.0
35	0.0011384756380444403	0.0	0.0	35.773181498632404	0.0
36	0.0011384756380444403	0.0	0.0	36.39393534027614	0.0
37	0.0011384756380444403	0.0	0.0	37.042297216142444	0.0
38	0.0011384756380444403	0.0	0.0	37.669312673795424	0.0
39	0.0011384756380444403	0.0	0.0	38.34699029734138	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	20	7.0265913E-4	45.000004	12
AGATCGG	20	7.0265913E-4	45.000004	1
GTCGAGG	20	7.0265913E-4	45.000004	1
AGGCGTA	20	7.0265913E-4	45.000004	22
TCTAGTT	20	7.0265913E-4	45.000004	12
ATCGTAG	20	7.0265913E-4	45.000004	38
CCGTACG	20	7.0265913E-4	45.000004	1
CGTAGTC	20	7.0265913E-4	45.000004	40
GTAGACG	20	7.0265913E-4	45.000004	1
TCGTAGT	20	7.0265913E-4	45.000004	39
ACGTCAT	20	7.0265913E-4	45.000004	25
TTACGCG	20	7.0265913E-4	45.000004	1
CGACTGG	20	7.0265913E-4	45.000004	2
TACGTCA	20	7.0265913E-4	45.000004	24
GTACGCG	20	7.0265913E-4	45.000004	11
CGAAGTA	20	7.0265913E-4	45.000004	16
TAGGTTG	95	0.0	45.0	1
TACGGGT	25	3.885258E-5	45.0	4
CACACGG	25	3.885258E-5	45.0	2
TACGGTT	60	0.0	44.999996	33
>>END_MODULE