SRR2935958.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935958.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259755
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1321	0.5085561394390868	RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1226	0.47198321495255147	RNA PCR Primer, Index 35 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	783	0.3014378933995496	TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	421	0.16207580219822526	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	399	0.15360628284344863	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	396	0.1524513483859791	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.14783161055610095	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	375	0.14436680718369233	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	315	0.12126811803430154	TruSeq Adapter, Index 10 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	305	0.11741833650940309	RNA PCR Primer, Index 35 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGGC	30	2.158993E-6	45.000004	4
CTACGAA	60	0.0	45.000004	11
AACCGCC	20	7.0231955E-4	45.0	25
TCGTTAG	20	7.0231955E-4	45.0	1
GGGTAAC	25	3.8824433E-5	45.0	7
ATTACCG	20	7.0231955E-4	45.0	1
GGATCCG	25	3.8824433E-5	45.0	8
CACGAGG	20	7.0231955E-4	45.0	2
CCTCGCC	25	3.8824433E-5	45.0	40
ACGGGAT	40	6.7793735E-9	45.0	5
CATTACA	35	1.2070814E-7	45.0	7
GTGTGAC	20	7.0231955E-4	45.0	22
GTTACAA	25	3.8824433E-5	45.0	9
CCACTCA	20	7.0231955E-4	45.0	40
CGGATGG	20	7.0231955E-4	45.0	2
TACGAGC	20	7.0231955E-4	45.0	24
AACGAAG	20	7.0231955E-4	45.0	35
AGCGACT	55	1.8189894E-12	45.0	19
GCGATGA	40	6.7793735E-9	45.0	9
CTCGCCA	25	3.8824433E-5	45.0	41