FastQCFastQC Report
Sat 14 Jan 2017
SRR2935958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259755
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC13210.5085561394390868RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC12260.47198321495255147RNA PCR Primer, Index 35 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT7830.3014378933995496TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC4210.16207580219822526No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG3990.15360628284344863No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC3960.1524513483859791RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.14783161055610095No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC3750.14436680718369233No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG3150.12126811803430154TruSeq Adapter, Index 10 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC3050.11741833650940309RNA PCR Primer, Index 35 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGC302.158993E-645.0000044
CTACGAA600.045.00000411
AACCGCC207.0231955E-445.025
TCGTTAG207.0231955E-445.01
GGGTAAC253.8824433E-545.07
ATTACCG207.0231955E-445.01
GGATCCG253.8824433E-545.08
CACGAGG207.0231955E-445.02
CCTCGCC253.8824433E-545.040
ACGGGAT406.7793735E-945.05
CATTACA351.2070814E-745.07
GTGTGAC207.0231955E-445.022
GTTACAA253.8824433E-545.09
CCACTCA207.0231955E-445.040
CGGATGG207.0231955E-445.02
TACGAGC207.0231955E-445.024
AACGAAG207.0231955E-445.035
AGCGACT551.8189894E-1245.019
GCGATGA406.7793735E-945.09
CTCGCCA253.8824433E-545.041