##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935958.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259755
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08270100671787	33.0	31.0	34.0	30.0	34.0
2	32.20515870724336	33.0	31.0	34.0	30.0	34.0
3	32.25460530114916	34.0	31.0	34.0	30.0	34.0
4	35.81952994167581	37.0	35.0	37.0	35.0	37.0
5	35.853107736135975	37.0	35.0	37.0	35.0	37.0
6	35.87611788031029	37.0	35.0	37.0	35.0	37.0
7	36.018313410713944	37.0	35.0	37.0	35.0	37.0
8	35.674042848068375	37.0	35.0	37.0	35.0	37.0
9	37.40964370271987	39.0	37.0	39.0	34.0	39.0
10	37.234324652076	39.0	37.0	39.0	34.0	39.0
11	37.21454832438259	39.0	37.0	39.0	34.0	39.0
12	37.03793189736482	39.0	37.0	39.0	34.0	39.0
13	36.97374449000019	39.0	37.0	39.0	33.0	39.0
14	38.13342572808993	40.0	37.0	41.0	33.0	41.0
15	38.28459894900964	40.0	37.0	41.0	34.0	41.0
16	38.39538026217012	40.0	37.0	41.0	34.0	41.0
17	38.35255529248715	40.0	37.0	41.0	34.0	41.0
18	38.351138572885986	40.0	37.0	41.0	34.0	41.0
19	38.33352582240958	40.0	37.0	41.0	34.0	41.0
20	38.21567246058786	40.0	37.0	41.0	34.0	41.0
21	38.20760331851167	40.0	37.0	41.0	34.0	41.0
22	38.181263113318316	40.0	37.0	41.0	34.0	41.0
23	38.209543608400224	40.0	37.0	41.0	34.0	41.0
24	38.11210948778657	40.0	36.0	41.0	34.0	41.0
25	38.04147754614926	40.0	36.0	41.0	34.0	41.0
26	37.9212527189082	40.0	36.0	41.0	34.0	41.0
27	37.85604511943947	40.0	36.0	41.0	34.0	41.0
28	37.80043887509384	40.0	36.0	41.0	33.0	41.0
29	37.8227599083752	40.0	36.0	41.0	34.0	41.0
30	37.7262766837982	40.0	36.0	41.0	33.0	41.0
31	37.66296317683972	40.0	36.0	41.0	33.0	41.0
32	37.61370522222864	40.0	35.0	41.0	33.0	41.0
33	37.59712036341938	40.0	35.0	41.0	33.0	41.0
34	37.52254239571904	40.0	35.0	41.0	33.0	41.0
35	37.50432522954323	40.0	35.0	41.0	33.0	41.0
36	37.40167080518181	40.0	35.0	41.0	33.0	41.0
37	37.310134549864294	40.0	35.0	41.0	33.0	41.0
38	37.251694866316335	39.0	35.0	41.0	32.0	41.0
39	37.25078246809493	39.0	35.0	41.0	33.0	41.0
40	37.17325171796501	39.0	35.0	41.0	32.0	41.0
41	37.074046697849894	39.0	35.0	41.0	32.0	41.0
42	37.047922080421934	39.0	35.0	41.0	32.0	41.0
43	36.95984677869531	39.0	35.0	41.0	32.0	41.0
44	36.87763084444958	39.0	35.0	41.0	32.0	41.0
45	36.77573097726704	39.0	35.0	41.0	31.0	41.0
46	36.66643567977517	39.0	35.0	41.0	31.0	41.0
47	36.59488749013494	39.0	35.0	41.0	31.0	41.0
48	36.53975091913534	39.0	35.0	41.0	31.0	41.0
49	36.483082135088836	39.0	35.0	41.0	31.0	41.0
50	36.35213181651941	38.0	35.0	40.0	31.0	41.0
51	35.2519066043002	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	7.0
11	3.0
12	3.0
13	7.0
14	9.0
15	13.0
16	18.0
17	24.0
18	59.0
19	110.0
20	148.0
21	266.0
22	414.0
23	631.0
24	851.0
25	1177.0
26	1573.0
27	2021.0
28	2363.0
29	2751.0
30	3465.0
31	4462.0
32	5999.0
33	8470.0
34	16925.0
35	22426.0
36	17739.0
37	27198.0
38	51631.0
39	88891.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.823218032376666	21.154549479317048	23.311967045870148	11.710265442436143
2	32.6484572000539	24.650921060229834	23.92523724278647	18.7753844969298
3	32.13335643202248	24.68441415949645	25.224538507439703	17.957690901041364
4	29.95861484860734	24.85341956843949	25.593347577524977	19.59461800542819
5	26.48380204423399	28.98500510096052	23.895208946892264	20.635983907913225
6	26.48842178206387	34.54100979769398	23.303112548362883	15.667455871879271
7	76.96521722392255	6.899578448923023	10.425208369425034	5.709995957729399
8	75.9011376104406	7.189082019595388	9.799233893476545	7.110546476487459
9	68.701661180728	9.468152682335278	12.494465939057958	9.33572019787877
10	39.691632499855636	27.042020365344264	19.11878500895074	14.147562125849358
11	32.50871013070008	24.759869877384457	23.65228773267117	19.07913225924429
12	30.64426093819176	21.434043618024674	28.48838328424862	19.433312159534946
13	24.692113722546246	25.873226694385092	28.9857750572655	20.44888452580316
14	18.790013666724413	30.791322592442878	28.297819098766148	22.12084464206656
15	18.335739446786395	27.090527612557985	35.41914496352332	19.154587977132298
16	20.428865661873687	25.943677696290735	31.918923601085638	21.708533040749938
17	20.02925833958923	27.262227868568456	27.558661045985637	25.14985274585667
18	22.114300013474235	27.35616253777598	28.837558468556914	21.691978980192875
19	22.8815614713865	27.59561894862467	27.263767781178416	22.259051798810418
20	25.996419703181843	27.245673808011396	27.35885738484341	19.39904910396335
21	24.861889087794268	27.568670477950374	27.89089719158438	19.67854324267098
22	21.75319050643876	26.664356797751726	26.799099151123173	24.78335354468634
23	22.459625416257627	26.456468595407213	27.978287232199573	23.10561875613559
24	22.945082866547324	24.85418952474447	28.404073068853343	23.796654539854863
25	20.430020596331158	28.105330022521223	26.692845181035974	24.771804200111642
26	21.369752266558873	27.797347500529344	27.549421570325883	23.2834786625859
27	23.982983965659948	28.0833862678293	26.964254778541317	20.969374987969434
28	19.58152874824354	27.24143904833401	30.32164924640527	22.85538295701719
29	22.382629785759658	25.391619025620294	29.369983253450364	22.85576793516968
30	23.60686031067737	26.076110180747243	28.322072722373004	21.994956786202383
31	24.942349521664646	26.419125714615692	26.907278011972817	21.731246751746838
32	24.530807876653	26.044541972243074	28.877981174568347	20.54666897653558
33	23.619564589709537	26.019133414178746	28.129968624280572	22.23133337183115
34	23.639968431791498	24.771804200111642	29.454293468845645	22.13393389925122
35	23.53717926507671	23.34930992666166	30.124925410482955	22.988585397778678
36	23.79973436507478	26.41720082385325	28.33400704510019	21.449057765971784
37	23.47789262959327	25.788146522684833	30.802871937017574	19.931088910704318
38	20.65176801216531	26.164655155819904	30.48680487382341	22.696771958191373
39	23.56335777944602	22.856922869627148	30.57727473965852	23.00244461126831
40	24.15468422167042	22.988585397778678	31.854247271467344	21.00248310908356
41	21.563396277261266	24.332544128120727	29.03851706415661	25.0655425304614
42	22.27984061904487	23.609170179592308	28.923023618409655	25.18796558295317
43	24.15198937460299	23.195318665665724	29.130911820754175	23.521780138977114
44	22.02767992916402	23.7269734942542	29.41618063174915	24.82916594483263
45	22.73488479528787	23.788569998652576	28.072606879559586	25.40393832649997
46	23.875190082962792	24.685184115801427	29.099728590402496	22.339897210833286
47	21.779753998960558	23.395507304960443	32.481761660025796	22.342977036053206
48	21.7289368828319	21.567246058786164	31.714885180266023	24.988931878115917
49	21.47754614925603	21.931050412889068	33.103886354449386	23.487517083405518
50	20.70374006275144	22.060403072125656	31.825373910030606	25.410482955092302
51	20.361879463340454	21.787068583857867	29.101653481164945	28.749398471636734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	221.0
1	349.0
2	477.0
3	709.5
4	942.0
5	712.5
6	483.0
7	441.5
8	400.0
9	393.0
10	386.0
11	409.5
12	433.0
13	459.5
14	486.0
15	436.5
16	387.0
17	453.0
18	519.0
19	523.5
20	528.0
21	687.0
22	846.0
23	929.0
24	1012.0
25	1319.0
26	1964.0
27	2302.0
28	2563.5
29	2825.0
30	3299.0
31	3773.0
32	4574.0
33	5375.0
34	5858.5
35	6342.0
36	6727.0
37	7112.0
38	7923.5
39	8735.0
40	9945.0
41	11155.0
42	12936.5
43	14718.0
44	16563.5
45	18409.0
46	21166.5
47	23924.0
48	26512.0
49	29100.0
50	29006.0
51	28912.0
52	24201.0
53	19490.0
54	17297.5
55	15105.0
56	13492.5
57	11880.0
58	11100.0
59	10320.0
60	10163.0
61	10006.0
62	9219.0
63	8432.0
64	6844.0
65	5256.0
66	4234.5
67	3213.0
68	2478.5
69	1744.0
70	1443.0
71	1142.0
72	946.5
73	751.0
74	628.0
75	379.5
76	254.0
77	199.0
78	144.0
79	97.5
80	51.0
81	39.5
82	28.0
83	15.5
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	259755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.49288752863275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.12964719800887	24.8795980828088
2	11.635434222127973	8.026794479413294
3	4.10393205129636	4.246694000115493
4	1.994486422536469	2.7518238339974204
5	1.1663336942085114	2.0115108467594465
6	0.7522573300445328	1.556851648668938
7	0.6774780405593938	1.6357721699293566
8	0.5524738551514001	1.5245134838597911
9	0.4788106744645468	1.4864006467632962
>10	6.367400694219673	45.51442705626456
>50	0.0959853566525665	2.2467324979307426
>100	0.04241213433485497	2.8369040056976766
>500	0.001116108798285657	0.3014378933995496
>1k	0.002232217596571314	0.9805393543916383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1321	0.5085561394390868	RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1226	0.47198321495255147	RNA PCR Primer, Index 35 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	783	0.3014378933995496	TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	421	0.16207580219822526	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	399	0.15360628284344863	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	396	0.1524513483859791	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.14783161055610095	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	375	0.14436680718369233	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	315	0.12126811803430154	TruSeq Adapter, Index 10 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	305	0.11741833650940309	RNA PCR Primer, Index 35 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.699563049796924E-4	0.0	0.0	0.3291563203788185	0.0
2	7.699563049796924E-4	0.0	0.0	1.687359242362996	0.0
3	7.699563049796924E-4	0.0	0.0	2.3114088275490365	0.0
4	7.699563049796924E-4	0.0	0.0	3.0605763122942773	0.0
5	7.699563049796924E-4	0.0	0.0	5.200669861985332	0.0
6	7.699563049796924E-4	0.0	0.0	6.162730265057458	0.0
7	7.699563049796924E-4	0.0	0.0	7.230659660064291	0.0
8	7.699563049796924E-4	0.0	0.0	8.779426767530943	0.0
9	7.699563049796924E-4	0.0	0.0	9.326865700371505	0.0
10	7.699563049796924E-4	0.0	0.0	10.747050104906547	0.0
11	7.699563049796924E-4	0.0	0.0	12.730072568381745	0.0
12	7.699563049796924E-4	0.0	0.0	14.386633558545553	0.0
13	7.699563049796924E-4	0.0	0.0	15.014532925256491	0.0
14	7.699563049796924E-4	0.0	0.0	15.296721911031549	0.0
15	7.699563049796924E-4	0.0	0.0	15.864564685954072	0.0
16	7.699563049796924E-4	0.0	0.0	16.905930588439105	0.0
17	7.699563049796924E-4	0.0	0.0	18.042386094589133	0.0
18	7.699563049796924E-4	0.0	0.0	19.231198629477777	0.0
19	7.699563049796924E-4	0.0	0.0	20.062751438855845	0.0
20	7.699563049796924E-4	0.0	0.0	20.82462320263325	0.0
21	7.699563049796924E-4	0.0	0.0	21.713152778579815	0.0
22	7.699563049796924E-4	0.0	0.0	22.659044099247367	0.0
23	7.699563049796924E-4	0.0	0.0	23.517160401147233	0.0
24	7.699563049796924E-4	0.0	0.0	24.22629015803353	0.0
25	7.699563049796924E-4	0.0	0.0	24.864198956709206	0.0
26	7.699563049796924E-4	0.0	0.0	25.420877365209524	0.0
27	7.699563049796924E-4	0.0	0.0	26.00796904775654	0.0
28	7.699563049796924E-4	0.0	0.0	26.56002771842698	0.0
29	7.699563049796924E-4	0.0	0.0	27.13903485977171	0.0
30	7.699563049796924E-4	0.0	0.0	27.820061211526244	0.0
31	7.699563049796924E-4	0.0	0.0	28.445265731169755	0.0
32	7.699563049796924E-4	0.0	0.0	29.06392562222094	0.0
33	7.699563049796924E-4	0.0	0.0	29.63138341899097	0.0
34	7.699563049796924E-4	0.0	0.0	30.166118072799367	0.0
35	7.699563049796924E-4	0.0	0.0	30.753209755346383	0.0
36	7.699563049796924E-4	0.0	0.0	31.36609497411022	0.0
37	7.699563049796924E-4	0.0	0.0	31.954341591114705	0.0
38	7.699563049796924E-4	0.0	0.0	32.4890762449231	0.0
39	7.699563049796924E-4	0.0	0.0	33.06346364843795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGGC	30	2.158993E-6	45.000004	4
CTACGAA	60	0.0	45.000004	11
AACCGCC	20	7.0231955E-4	45.0	25
TCGTTAG	20	7.0231955E-4	45.0	1
GGGTAAC	25	3.8824433E-5	45.0	7
ATTACCG	20	7.0231955E-4	45.0	1
GGATCCG	25	3.8824433E-5	45.0	8
CACGAGG	20	7.0231955E-4	45.0	2
CCTCGCC	25	3.8824433E-5	45.0	40
ACGGGAT	40	6.7793735E-9	45.0	5
CATTACA	35	1.2070814E-7	45.0	7
GTGTGAC	20	7.0231955E-4	45.0	22
GTTACAA	25	3.8824433E-5	45.0	9
CCACTCA	20	7.0231955E-4	45.0	40
CGGATGG	20	7.0231955E-4	45.0	2
TACGAGC	20	7.0231955E-4	45.0	24
AACGAAG	20	7.0231955E-4	45.0	35
AGCGACT	55	1.8189894E-12	45.0	19
GCGATGA	40	6.7793735E-9	45.0	9
CTCGCCA	25	3.8824433E-5	45.0	41
>>END_MODULE