SRR2935957.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935957.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313726
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1435	0.45740550671605157	TruSeq Adapter, Index 18 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1074	0.3423369437024665	RNA PCR Primer, Index 34 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	835	0.26615581749679657	RNA PCR Primer, Index 34 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	466	0.14853725862695472	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	460	0.1466247617347622	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	455	0.14503101432460172	TruSeq Adapter, Index 18 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	451	0.14375601639647337	RNA PCR Primer, Index 34 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	450	0.14343726691444125	No Hit
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	378	0.12048730420813067	TruSeq Adapter, Index 3 (95% over 24bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	376	0.11984980524406648	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	368	0.11729980938780975	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAATC	20	7.025436E-4	45.000004	27
ATTAGCG	20	7.025436E-4	45.000004	1
TACTTAT	25	3.8842998E-5	45.000004	23
TTTACGG	20	7.025436E-4	45.000004	2
GAGTCGT	20	7.025436E-4	45.000004	39
TCCGACC	20	7.025436E-4	45.000004	29
CTATAAC	25	3.8842998E-5	45.000004	34
CACGCGA	20	7.025436E-4	45.000004	22
GAATCCG	25	3.8842998E-5	45.000004	9
CGCGATA	20	7.025436E-4	45.000004	24
CCGCTTA	20	7.025436E-4	45.000004	16
TACGGGC	45	3.8198777E-10	45.000004	4
CGACAGG	35	1.2081546E-7	45.000004	2
CCTCGTG	40	6.7884685E-9	45.000004	15
ACGGGCC	50	2.1827873E-11	45.000004	5
CGCCTTG	20	7.025436E-4	45.000004	15
GTTACGG	20	7.025436E-4	45.000004	2
CCCCGGG	25	3.8842998E-5	45.000004	17
ATGAGCG	20	7.025436E-4	45.000004	1
GTCGTAA	20	7.025436E-4	45.000004	41