##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935957.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313726
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05772871869083	33.0	31.0	34.0	30.0	34.0
2	32.163818108795574	33.0	31.0	34.0	30.0	34.0
3	32.22206957663694	34.0	31.0	34.0	30.0	34.0
4	35.805986752771524	37.0	35.0	37.0	35.0	37.0
5	35.83522564275833	37.0	35.0	37.0	35.0	37.0
6	35.84740187297196	37.0	35.0	37.0	35.0	37.0
7	36.00782529978389	37.0	35.0	37.0	35.0	37.0
8	35.683322389601116	37.0	35.0	37.0	35.0	37.0
9	37.452107890324676	39.0	37.0	39.0	34.0	39.0
10	37.19151106379452	39.0	37.0	39.0	34.0	39.0
11	37.14451782765853	39.0	37.0	39.0	34.0	39.0
12	36.994425071559256	39.0	35.0	39.0	33.0	39.0
13	36.90637690213753	39.0	35.0	39.0	33.0	39.0
14	38.03366950778705	40.0	37.0	41.0	33.0	41.0
15	38.19913555140473	40.0	37.0	41.0	33.0	41.0
16	38.31625048609296	40.0	37.0	41.0	34.0	41.0
17	38.280735418804944	40.0	37.0	41.0	34.0	41.0
18	38.298333577707936	40.0	37.0	41.0	34.0	41.0
19	38.29569751949153	40.0	37.0	41.0	34.0	41.0
20	38.15328981340405	40.0	37.0	41.0	34.0	41.0
21	38.129351727303444	40.0	36.0	41.0	34.0	41.0
22	38.13688059006904	40.0	36.0	41.0	34.0	41.0
23	38.16539910622645	40.0	36.0	41.0	34.0	41.0
24	38.08214174151967	40.0	36.0	41.0	34.0	41.0
25	38.007583050177544	40.0	36.0	41.0	34.0	41.0
26	37.82402478595972	40.0	36.0	41.0	34.0	41.0
27	37.73491518076283	40.0	36.0	41.0	33.0	41.0
28	37.745752663151926	40.0	36.0	41.0	33.0	41.0
29	37.740091672351035	40.0	36.0	41.0	33.0	41.0
30	37.603440581909055	40.0	36.0	41.0	33.0	41.0
31	37.62403817343797	40.0	36.0	41.0	33.0	41.0
32	37.54986198147428	40.0	35.0	41.0	33.0	41.0
33	37.51926521869402	40.0	35.0	41.0	33.0	41.0
34	37.4733971682296	40.0	35.0	41.0	33.0	41.0
35	37.446290712277595	40.0	35.0	41.0	33.0	41.0
36	37.373188706068355	40.0	35.0	41.0	33.0	41.0
37	37.315220925266	40.0	35.0	41.0	33.0	41.0
38	37.28339060199027	40.0	35.0	41.0	33.0	41.0
39	37.276639487960836	40.0	35.0	41.0	33.0	41.0
40	37.19338212325405	39.0	35.0	41.0	33.0	41.0
41	37.11062519523406	39.0	35.0	41.0	32.0	41.0
42	37.10665357668794	39.0	35.0	41.0	32.0	41.0
43	37.048347921434626	39.0	35.0	41.0	32.0	41.0
44	36.975701726984695	39.0	35.0	41.0	32.0	41.0
45	36.86241816107049	39.0	35.0	41.0	32.0	41.0
46	36.805572378444886	39.0	35.0	41.0	32.0	41.0
47	36.77354124299548	39.0	35.0	41.0	31.0	41.0
48	36.734864180845705	39.0	35.0	41.0	32.0	41.0
49	36.69618393120111	39.0	35.0	41.0	31.0	41.0
50	36.589817229047	39.0	35.0	41.0	31.0	41.0
51	35.63726308944748	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	11.0
10	6.0
11	10.0
12	6.0
13	7.0
14	8.0
15	12.0
16	20.0
17	30.0
18	53.0
19	115.0
20	203.0
21	314.0
22	441.0
23	681.0
24	991.0
25	1422.0
26	1841.0
27	2271.0
28	2868.0
29	3432.0
30	4309.0
31	5589.0
32	7197.0
33	10506.0
34	20891.0
35	27622.0
36	21616.0
37	32176.0
38	59879.0
39	109093.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17111109694447	22.079457870880958	23.92310487495458	10.826326157219993
2	31.81056080783869	25.7916143386267	24.447129023415336	17.950695830119276
3	32.105722828200406	25.10152171002722	25.469358612292254	17.32339684948012
4	29.977432536672126	25.761970636797717	25.052434289794277	19.20816253673588
5	25.761333137833653	31.389173992592262	23.645155326622593	19.204337542951492
6	26.312132242785108	34.541287620407616	23.913861139975648	15.232718996831629
7	78.38209137910151	6.967863677221524	9.296328643465955	5.353716300211012
8	77.77200487049208	6.697245366976279	9.242459981002531	6.288289781529105
9	71.46459012004107	8.266767816502298	11.705755978146536	8.562886085310112
10	40.596890280053294	25.84357050419793	18.702944607715015	14.856594608033763
11	31.87654195061933	24.351504178805712	23.861267475440354	19.910686395134608
12	29.731676686025388	21.650102318583734	28.91886550684355	19.699355488547333
13	24.426729056565282	25.687702007484237	29.143265142194142	20.742303793756335
14	19.267449940393846	29.163346359562166	28.844915627012107	22.724288073031882
15	18.67521340277822	26.020795216207777	35.08889923053875	20.215092150475257
16	21.63416484448213	24.732409809834056	31.407023963586063	22.226401382097755
17	20.456385508373547	26.22288238781612	28.523297399641727	24.797434704168605
18	22.14671401158973	26.251569841199007	29.781401605222392	21.820314541988868
19	23.526261769824625	27.360499289188656	27.409586709421596	21.703652231565123
20	25.184715324837597	27.214512026417957	27.70124248548096	19.899530163263485
21	22.901831534523755	28.566966078680124	28.650797192454565	19.88040519434156
22	21.38299025264084	25.772489369704775	27.78539234873743	25.059128028916955
23	21.217559271466182	26.057770156123496	29.00715911336644	23.71751145904388
24	22.230863874846204	25.632558347092687	29.631270599185278	22.50530717887583
25	21.83688951505454	27.566411454581385	26.09506384552125	24.501635184842822
26	20.847172373344893	26.9636561840587	28.40759133766408	23.781580104932328
27	21.859839477760847	27.61900511911668	28.162154236499365	22.359001166623106
28	20.035317442609156	27.421380440256787	29.89041392807737	22.652888189056693
29	23.450399393100987	24.437885288436405	28.39101636459841	23.7206989538642
30	22.998731377061514	26.207263663196546	28.8289781529105	21.96502680683144
31	25.905089154230126	27.453255388459997	23.95242982730153	22.68922563000835
32	25.937282851915366	28.071948133084284	24.354691673626032	21.63607734137432
33	23.17978108285574	26.5276068926388	25.97170779597483	24.32090422853063
34	22.47725722445701	26.906281277292926	26.426882056316657	24.189579441933407
35	23.302499633438096	27.172118345307688	25.472546107112574	24.05283591414164
36	23.714323964223556	27.819179793832838	25.61757712143718	22.848919120506427
37	22.913625265358945	28.80124694797371	27.476842850130367	20.808284936536978
38	22.654800685948885	27.803879818695293	25.080484244213103	24.46083525114272
39	23.5488929830489	25.68228326628969	26.187182445828526	24.58164130483288
40	22.49574469441487	25.781733104683706	28.214110402070595	23.508411798830828
41	20.034361194163058	27.305355628797102	26.62355048673046	26.03673269030938
42	22.095395344982567	25.298827639405086	27.65279256421208	24.95298445140027
43	24.057298406890087	23.620930365988155	27.739811172806846	24.581960054314912
44	23.297399641725583	24.435654042062183	26.48808195686682	25.778864359345416
45	22.604119518305783	24.605228766503252	26.031632698596862	26.7590190165941
46	23.236837240139486	26.49573194443559	26.548006859488854	23.719423955936072
47	20.955547197235806	25.376283763538883	30.141588519918656	23.526580519306656
48	21.27493417823196	24.125192046562923	28.483772463869744	26.11610131133537
49	21.816170798722453	23.017218847019375	30.043732428934806	25.122877925323372
50	21.349202807545435	22.75170052848664	29.97169504599555	25.92740161797237
51	20.109904821404665	23.806123815048803	26.687937882100943	29.396033481445592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	219.0
1	380.0
2	541.0
3	789.5
4	1038.0
5	748.0
6	458.0
7	447.5
8	437.0
9	470.0
10	503.0
11	499.0
12	495.0
13	539.5
14	584.0
15	498.0
16	412.0
17	484.0
18	556.0
19	674.0
20	792.0
21	840.0
22	888.0
23	936.5
24	985.0
25	1486.0
26	2352.5
27	2718.0
28	3202.0
29	3686.0
30	4439.5
31	5193.0
32	5616.0
33	6039.0
34	6753.0
35	7467.0
36	8351.0
37	9235.0
38	9558.0
39	9881.0
40	11747.5
41	13614.0
42	15039.0
43	16464.0
44	17916.5
45	19369.0
46	20587.0
47	21805.0
48	25712.5
49	29620.0
50	32241.0
51	34862.0
52	34018.5
53	33175.0
54	27860.5
55	22546.0
56	19913.0
57	17280.0
58	15464.0
59	13648.0
60	12571.5
61	11495.0
62	9919.5
63	8344.0
64	7327.0
65	6310.0
66	5179.0
67	4048.0
68	3350.5
69	2653.0
70	2199.0
71	1745.0
72	1517.0
73	1289.0
74	941.5
75	491.5
76	389.0
77	281.0
78	173.0
79	151.0
80	129.0
81	80.0
82	31.0
83	24.5
84	18.0
85	12.0
86	6.0
87	5.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	313726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.01690525633382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.57598013097875	22.59624469226445
2	11.956996633786456	7.656520567484868
3	4.528026632572407	4.349201990796811
4	2.146999638090843	2.7496113599255008
5	1.3389996258076626	2.1435312078875186
6	0.9059997449712636	1.7404384798204529
7	0.6699998113776443	1.5015924337847697
8	0.5479998457234309	1.403620731281011
9	0.47099986740097205	1.3571962317290442
>10	6.640998130381856	46.58298318835038
>50	0.1579999555187974	3.31022690253517
>100	0.05599998423451046	3.5381872037833606
>500	9.999997184734011E-4	0.2673410836267402
>1k	0.0019999994369468022	0.8033039267299296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1435	0.45740550671605157	TruSeq Adapter, Index 18 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1074	0.3423369437024665	RNA PCR Primer, Index 34 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	835	0.26615581749679657	RNA PCR Primer, Index 34 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	466	0.14853725862695472	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	460	0.1466247617347622	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	455	0.14503101432460172	TruSeq Adapter, Index 18 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	451	0.14375601639647337	RNA PCR Primer, Index 34 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	450	0.14343726691444125	No Hit
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	378	0.12048730420813067	TruSeq Adapter, Index 3 (95% over 24bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	376	0.11984980524406648	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	368	0.11729980938780975	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.187494820320917E-4	0.0	0.0	0.27476205351166305	0.0
2	3.187494820320917E-4	0.0	0.0	1.3849664994294384	0.0
3	3.187494820320917E-4	0.0	0.0	1.9520218279645296	0.0
4	3.187494820320917E-4	0.0	0.0	2.6405207091538476	0.0
5	3.187494820320917E-4	0.0	0.0	4.595411282456666	0.0
6	3.187494820320917E-4	0.0	0.0	5.565047206798289	0.0
7	3.187494820320917E-4	0.0	0.0	6.663457921880877	0.0
8	3.187494820320917E-4	0.0	0.0	8.297686516259411	0.0
9	3.187494820320917E-4	0.0	0.0	8.870798084953112	0.0
10	3.187494820320917E-4	0.0	0.0	10.30899574788191	0.0
11	3.187494820320917E-4	0.0	0.0	12.179417708446223	0.0
12	3.187494820320917E-4	0.0	0.0	13.78049635669342	0.0
13	3.187494820320917E-4	0.0	0.0	14.392495362195037	0.0
14	3.187494820320917E-4	0.0	0.0	14.637613713877714	0.0
15	3.187494820320917E-4	0.0	0.0	15.159725365446281	0.0
16	3.187494820320917E-4	0.0	0.0	16.124580047557423	0.0
17	3.187494820320917E-4	0.0	0.0	17.27462817872921	0.0
18	3.187494820320917E-4	0.0	0.0	18.47089498479565	0.0
19	3.187494820320917E-4	0.0	0.0	19.198600052274916	0.0
20	3.187494820320917E-4	0.0	0.0	19.931723860948726	0.0
21	3.187494820320917E-4	0.0	0.0	20.812109930321363	0.0
22	3.187494820320917E-4	0.0	0.0	21.71098346965186	0.0
23	3.187494820320917E-4	0.0	0.0	22.569057075282252	0.0
24	3.187494820320917E-4	0.0	0.0	23.32194335184205	0.0
25	3.187494820320917E-4	0.0	0.0	23.929798614077253	0.0
26	3.187494820320917E-4	0.0	0.0	24.502910182770954	0.0
27	3.187494820320917E-4	0.0	0.0	25.09195922556626	0.0
28	3.187494820320917E-4	0.0	0.0	25.66060830151151	0.0
29	3.187494820320917E-4	0.0	0.0	26.263044822552164	0.0
30	6.374989640641834E-4	0.0	0.0	26.872493832197524	0.0
31	9.562484460962751E-4	0.0	0.0	27.49692406749839	0.0
32	9.562484460962751E-4	0.0	0.0	28.087248108221825	0.0
33	9.562484460962751E-4	0.0	0.0	28.666097167592103	0.0
34	9.562484460962751E-4	0.0	0.0	29.25546495986944	0.0
35	9.562484460962751E-4	0.0	0.0	29.870332710709345	0.0
36	9.562484460962751E-4	0.0	0.0	30.432288047531923	0.0
37	9.562484460962751E-4	0.0	0.0	31.001255872959206	0.0
38	0.0015937474101604585	0.0	0.0	31.590942414718576	0.0
39	0.0015937474101604585	0.0	0.0	32.234178869459335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAATC	20	7.025436E-4	45.000004	27
ATTAGCG	20	7.025436E-4	45.000004	1
TACTTAT	25	3.8842998E-5	45.000004	23
TTTACGG	20	7.025436E-4	45.000004	2
GAGTCGT	20	7.025436E-4	45.000004	39
TCCGACC	20	7.025436E-4	45.000004	29
CTATAAC	25	3.8842998E-5	45.000004	34
CACGCGA	20	7.025436E-4	45.000004	22
GAATCCG	25	3.8842998E-5	45.000004	9
CGCGATA	20	7.025436E-4	45.000004	24
CCGCTTA	20	7.025436E-4	45.000004	16
TACGGGC	45	3.8198777E-10	45.000004	4
CGACAGG	35	1.2081546E-7	45.000004	2
CCTCGTG	40	6.7884685E-9	45.000004	15
ACGGGCC	50	2.1827873E-11	45.000004	5
CGCCTTG	20	7.025436E-4	45.000004	15
GTTACGG	20	7.025436E-4	45.000004	2
CCCCGGG	25	3.8842998E-5	45.000004	17
ATGAGCG	20	7.025436E-4	45.000004	1
GTCGTAA	20	7.025436E-4	45.000004	41
>>END_MODULE