##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935956.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028323
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.960793447195094	33.0	31.0	34.0	30.0	34.0
2	32.09693063366277	33.0	31.0	34.0	30.0	34.0
3	32.15051982694153	33.0	31.0	34.0	30.0	34.0
4	35.77912484695956	37.0	35.0	37.0	35.0	37.0
5	35.73689200766685	37.0	35.0	37.0	35.0	37.0
6	35.730649805557206	37.0	35.0	37.0	35.0	37.0
7	35.8797109468523	37.0	35.0	37.0	35.0	37.0
8	35.333702542878065	37.0	35.0	37.0	32.0	37.0
9	37.202456815611434	39.0	37.0	39.0	34.0	39.0
10	37.04491779333925	39.0	37.0	39.0	33.0	39.0
11	36.94462148566161	39.0	37.0	39.0	33.0	39.0
12	36.71279354833063	39.0	35.0	39.0	33.0	39.0
13	36.56029185382414	39.0	35.0	39.0	32.0	39.0
14	37.48609823956092	40.0	36.0	41.0	32.0	41.0
15	37.617207822833876	40.0	36.0	41.0	32.0	41.0
16	37.93347809977993	40.0	36.0	41.0	33.0	41.0
17	37.79555548208102	40.0	36.0	41.0	33.0	41.0
18	37.78223476475777	40.0	36.0	41.0	33.0	41.0
19	37.78194594499977	40.0	36.0	41.0	33.0	41.0
20	37.3039327137485	39.0	35.0	41.0	32.0	41.0
21	37.48116982699016	39.0	35.0	41.0	32.0	41.0
22	37.45118897467041	39.0	35.0	41.0	32.0	41.0
23	37.2808913152774	39.0	35.0	41.0	32.0	41.0
24	37.30018680900845	39.0	35.0	41.0	32.0	41.0
25	37.39992881614045	39.0	35.0	41.0	33.0	41.0
26	37.08606731542521	39.0	35.0	41.0	32.0	41.0
27	36.95320341954814	39.0	35.0	41.0	31.0	41.0
28	36.937214279949	39.0	35.0	41.0	31.0	41.0
29	36.860024525368004	39.0	35.0	41.0	31.0	41.0
30	36.79397815666867	39.0	35.0	41.0	31.0	41.0
31	36.80897539002823	39.0	35.0	41.0	31.0	41.0
32	36.47762035858383	39.0	35.0	41.0	30.0	41.0
33	36.39384123470933	39.0	35.0	41.0	30.0	41.0
34	36.074750832180165	39.0	35.0	41.0	29.0	41.0
35	35.966506632643636	39.0	35.0	41.0	29.0	41.0
36	35.923590156011294	39.0	35.0	41.0	28.0	41.0
37	35.74876181900045	39.0	35.0	41.0	28.0	41.0
38	35.70467353156547	39.0	35.0	41.0	27.0	41.0
39	35.57056294568925	39.0	35.0	41.0	27.0	41.0
40	35.49042275627405	38.0	35.0	41.0	26.0	41.0
41	35.41233736870613	38.0	35.0	41.0	25.0	41.0
42	35.32788433206298	38.0	35.0	41.0	26.0	41.0
43	35.29174977122947	38.0	35.0	41.0	26.0	41.0
44	35.40014275670193	38.0	35.0	40.0	26.0	41.0
45	35.36488437971338	38.0	35.0	40.0	26.0	41.0
46	35.31176974549825	38.0	35.0	40.0	26.0	41.0
47	35.09444795069253	38.0	35.0	40.0	26.0	41.0
48	35.073307705847284	38.0	35.0	40.0	25.0	41.0
49	35.10996253122803	38.0	35.0	40.0	26.0	41.0
50	34.949397222468036	37.0	34.0	40.0	25.0	41.0
51	33.99426250312402	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	18.0
9	19.0
10	27.0
11	23.0
12	23.0
13	23.0
14	34.0
15	53.0
16	81.0
17	154.0
18	430.0
19	1132.0
20	2519.0
21	4748.0
22	7401.0
23	9264.0
24	10534.0
25	11914.0
26	13141.0
27	13562.0
28	13583.0
29	14637.0
30	17454.0
31	21955.0
32	29078.0
33	41932.0
34	78239.0
35	76788.0
36	73900.0
37	104270.0
38	178409.0
39	302740.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.761624509030725	18.486312180122393	20.59761378477385	12.154449526073034
2	31.148092574025867	32.98807864844023	20.529833525069456	15.33399525246445
3	27.316417117967802	33.28331662327887	24.339920433560273	15.060345825193059
4	25.348358443796354	21.411852112614422	36.14234049029342	17.097448953295803
5	32.22090724412466	24.617556934931923	24.014633534405046	19.146902286538374
6	23.237445821983947	38.205797205741774	24.721026370119116	13.835730602155161
7	70.19399546640501	5.002708293016883	19.96376624854253	4.839529992035577
8	69.02957533771004	13.583475231031494	11.705757821229321	5.681191610029145
9	64.27367665607012	5.751597503897122	12.297789702262811	17.676936137769943
10	46.822642302078236	18.952313621303812	19.54026118252728	14.68478289409067
11	36.695085104582894	20.334077911317745	24.49298518072629	18.477851803373067
12	28.219538024531204	17.911201052587565	28.78521631821908	25.084044604662154
13	29.635143821542453	18.76171203016951	33.73239731096163	17.870746837326404
14	20.359556287275495	27.518688194273587	30.755025415166248	21.366730103284667
15	17.819595593991384	21.286113409891637	37.38309850115187	23.5111924949651
16	23.181335047451043	23.356960799281936	27.178814438654005	26.282889714613017
17	23.63838988333432	27.22451992224233	28.742330960213863	20.394759234209484
18	23.451678120590515	22.677602270881813	28.92486115743789	24.945858451089784
19	22.259542964613257	25.195196450920577	26.671191833694273	25.87406875077189
20	25.691830290677153	27.359205230263257	29.516406809922564	17.432557669137033
21	22.26810058707235	30.565493526839333	29.579130292719313	17.587275593369007
22	21.09891541859902	20.766140599792088	34.66216354199994	23.472780439608957
23	24.103029884579065	24.93837053143808	31.08138201712886	19.877217566853993
24	23.238904507630384	26.296212376850463	26.96837472272817	23.496508392790982
25	21.924628740191554	32.013773882330746	24.654413058931873	21.407184318545827
26	20.14892207993014	22.968172451651863	30.743550421414284	26.139355047003715
27	25.181290314424555	23.835993165571516	28.07104382572402	22.91167269427991
28	18.39003892745762	27.471621270748585	29.137051296139443	25.00128850565435
29	22.258181524676584	25.778767955204735	29.419744574418736	22.54330594569994
30	22.68586815621162	27.36951327549807	30.412137042544025	19.532481525746288
31	26.437996621684047	21.556748220160397	26.180878965072257	25.824376193083303
32	28.96726028689429	23.764614814605917	25.341940226952037	21.92618467154775
33	27.99859577195103	20.77032216531187	27.225686870759482	24.00539519197762
34	26.005350458951128	23.35054258243762	27.779014959307535	22.865091999303722
35	24.164002944600092	23.402277300031216	31.069420794828083	21.36429896054061
36	27.381377252089077	28.335163173438694	25.28923305226082	18.994226522211406
37	22.47396975463935	23.475308828062776	30.599724016675694	23.450997400622178
38	25.68609279380117	22.29941370561584	27.023318548743923	24.991174951839064
39	25.997279065040846	22.77270857502944	25.966257683626644	25.26375467630307
40	23.409862465392685	20.238582624331073	32.86992511107891	23.481629799197336
41	25.05623233167011	22.407356443452105	26.294170216945457	26.242241007932332
42	22.508200244475717	21.23904648636664	33.24772469350584	23.005028575651814
43	25.89779670395391	21.968000326745585	27.473371693524314	24.66083127577619
44	23.68808244102291	20.758263697301334	29.91861506549985	25.63503879617591
45	22.3884907757582	20.035630828056945	28.219149041692155	29.356729354492707
46	26.67692933057026	23.384675826564223	26.806752353103064	23.13164248976246
47	19.374262755962864	23.76033600337637	34.975294727434864	21.890106513225902
48	22.47319178896125	24.51369851690568	28.34381804160755	24.66929165252552
49	22.79371364833812	19.91844974779325	34.094832071246095	23.19300453262253
50	23.22538735397341	21.279889684466845	30.4248762305229	25.069846731036844
51	21.519794850450687	21.254314062799335	27.94744452861601	29.278446558133968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	540.0
1	961.5
2	1383.0
3	6630.5
4	11878.0
5	7594.0
6	3310.0
7	3175.5
8	3041.0
9	3141.0
10	3241.0
11	3113.5
12	2986.0
13	2935.0
14	2884.0
15	2746.5
16	2609.0
17	2578.5
18	2548.0
19	2436.0
20	2324.0
21	2487.0
22	2650.0
23	3097.0
24	3544.0
25	4084.0
26	5348.5
27	6073.0
28	6993.0
29	7913.0
30	9157.0
31	10401.0
32	12443.0
33	14485.0
34	17382.0
35	20279.0
36	21205.5
37	22132.0
38	24224.0
39	26316.0
40	28927.5
41	31539.0
42	34845.0
43	38151.0
44	44369.5
45	50588.0
46	96297.0
47	142006.0
48	119058.0
49	96110.0
50	96146.0
51	96182.0
52	86463.5
53	76745.0
54	72167.5
55	67590.0
56	64780.0
57	61970.0
58	58899.0
59	55828.0
60	48791.0
61	41754.0
62	38341.5
63	34929.0
64	30216.0
65	25503.0
66	21953.5
67	18404.0
68	15644.5
69	12885.0
70	10922.5
71	8960.0
72	7549.5
73	6139.0
74	5019.0
75	3003.0
76	2107.0
77	1597.0
78	1087.0
79	764.0
80	441.0
81	275.0
82	109.0
83	133.5
84	158.0
85	100.0
86	42.0
87	33.5
88	25.0
89	16.0
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1028323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.702482905499462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34165550599661	21.48726786022396
2	9.867654213997367	5.861876612172732
3	3.7297292548375522	3.3234665830186096
4	2.0549592759757114	2.441495710646645
5	1.294897591619034	1.9230836789718393
6	1.0022411636664794	1.7861430618594896
7	0.8430019082276893	1.7527474837905455
8	0.6922652807478654	1.6449598133987406
9	0.6211308007077505	1.6604214291091035
>10	7.426094822358061	44.08308552129533
>50	0.07993497664272493	1.5702671564154966
>100	0.03714816417843731	2.11880955765952
>500	0.002985120335759272	0.679374655209878
>1k	0.0049752005595987865	3.291262830807948
>5k	3.3168003730658577E-4	0.7275495691306482
>10k+	9.950401119197573E-4	5.648188476289549
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	22068	2.1460183230366336	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC	18505	1.799531859153204	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG	16759	1.6297408499080541	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7385	0.7181595665953208	No Hit
GAACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT	4672	0.45433195600993076	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	4220	0.4103768951973261	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCTGTGT	3759	0.3655466229968599	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT	3445	0.3350114701314665	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC	2839	0.2760805700154524	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTA	2581	0.25099117689675327	No Hit
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	1692	0.16453974091798004	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1677	0.16308105527154407	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT	1614	0.15695457555651288	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT	1261	0.12262684001038585	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	1232	0.11980671442727625	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1193	0.11601413174654267	TruSeq Adapter, Index 15 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1183	0.11504167464891867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.862285488119978E-4	0.0	0.0	0.12126540007371225	0.0
2	4.862285488119978E-4	0.0	0.0	0.735761040062315	0.0
3	5.834742585743973E-4	0.0	0.0	1.0707725101937815	0.0
4	5.834742585743973E-4	0.0	0.0	1.9028068029208722	0.0
5	5.834742585743973E-4	0.0	0.0	5.031298531687028	0.0
6	5.834742585743973E-4	0.0	0.0	6.1360097945878875	0.0
7	5.834742585743973E-4	0.0	0.0	7.311710425615298	0.0
8	5.834742585743973E-4	0.0	0.0	8.704074497993334	0.0
9	6.807199683367969E-4	0.0	0.0	9.440516258023987	0.0
10	6.807199683367969E-4	0.0	0.0	12.128290430146947	0.0
11	6.807199683367969E-4	0.0	0.0	13.30933957521129	0.0
12	6.807199683367969E-4	0.0	0.0	16.09358149141855	0.0
13	6.807199683367969E-4	0.0	0.0	16.58593651994558	0.0
14	6.807199683367969E-4	0.0	0.0	16.93446514373402	0.0
15	6.807199683367969E-4	0.0	0.0	17.477290695627737	0.0
16	6.807199683367969E-4	0.0	0.0	18.009516465157347	0.0
17	6.807199683367969E-4	0.0	0.0	18.518306018634224	0.0
18	6.807199683367969E-4	0.0	0.0	19.011050030000302	0.0
19	6.807199683367969E-4	0.0	0.0	19.793002782199757	0.0
20	6.807199683367969E-4	0.0	0.0	20.237804658652973	0.0
21	6.807199683367969E-4	0.0	0.0	20.68630187207716	0.0
22	6.807199683367969E-4	0.0	0.0	21.2779447702716	0.0
23	6.807199683367969E-4	0.0	0.0	21.712244110070476	0.0
24	6.807199683367969E-4	0.0	0.0	22.109006605901065	0.0
25	6.807199683367969E-4	0.0	0.0	22.46959369770004	0.0
26	6.807199683367969E-4	0.0	0.0	22.823081852686364	0.0
27	6.807199683367969E-4	0.0	0.0	23.31932670960389	0.0
28	6.807199683367969E-4	0.0	0.0	23.645488820146976	0.0
29	7.779656780991964E-4	0.0	0.0	24.040792630331133	0.0
30	7.779656780991964E-4	0.0	0.0	24.57817242247815	0.0
31	8.75211387861596E-4	0.0	0.0	24.979894449506624	0.0
32	8.75211387861596E-4	0.0	0.0	25.376559699627453	0.0
33	8.75211387861596E-4	0.0	0.0	25.74191183120479	0.0
34	8.75211387861596E-4	0.0	0.0	26.140716486940388	0.0
35	8.75211387861596E-4	0.0	0.0	26.578224935161423	0.0
36	8.75211387861596E-4	0.0	0.0	26.973820482474864	0.0
37	9.724570976239956E-4	0.0	0.0	27.40131262259037	0.0
38	9.724570976239956E-4	0.0	0.0	27.92682843814638	0.0
39	9.724570976239956E-4	0.0	0.0	28.91640078068856	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	20	7.032938E-4	45.000004	11
CGAATGT	20	7.032938E-4	45.000004	45
TGTAGCG	65	0.0	45.000004	1
CGATCGC	35	1.2117562E-7	45.0	34
CGATGAA	3365	0.0	43.261513	19
CCGATGA	3335	0.0	42.908546	18
GATGAAT	3490	0.0	42.872494	20
CCCGTCG	295	0.0	42.711864	40
GTCGAAT	475	0.0	42.157898	43
CGTTTTT	3060	0.0	42.132355	1
CGGTCGA	145	0.0	41.896553	41
ATGAATG	3590	0.0	41.80362	21
ACCGATG	3435	0.0	41.65939	17
CACCGAT	3580	0.0	41.606148	16
TGAATGA	3610	0.0	41.509697	22
TACGGCT	2165	0.0	41.362587	7
CCGTCGA	240	0.0	41.249996	41
GGTCGAA	155	0.0	40.64516	42
CGCGTAA	50	1.0804797E-9	40.5	41
ACGGCTG	2215	0.0	40.428894	8
>>END_MODULE