SRR2935954.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935954.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466622
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC	16054	3.4404721594781216	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG	14185	3.039933822237271	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	13208	2.8305566389925896	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	4474	0.9588060571511845	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACAACGTT	3371	0.7224262893734115	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	3053	0.6542769093613245	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTA	2313	0.49569030178602813	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTC	2098	0.44961446309861086	No Hit
GAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	2012	0.43118412762364394	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	1174	0.25159550985594337	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	946	0.20273369022463578	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	790	0.16930191889795165	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACAACGATCGAATGC	751	0.16094397606628064	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATG	731	0.15665785153721856	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACAACGT	716	0.153443258140422	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	709	0.15194311455525028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	673	0.14422809040293857	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	654	0.1401562721003296	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACAACGTTCGT	638	0.13672737247707997	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTAT	597	0.1279408171925027	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACACCGATCGAATGC	537	0.11508244360531651	No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	520	0.11143923775561375	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	20	7.0289726E-4	45.000004	35
GATCGAG	30	2.1627184E-6	45.000004	1
GAAGACG	20	7.0289726E-4	45.000004	1
CGTGCGT	20	7.0289726E-4	45.000004	11
GCGAAAT	20	7.0289726E-4	45.000004	22
ATAGCGG	20	7.0289726E-4	45.000004	2
CGACAGG	30	2.1627184E-6	45.000004	2
AGCGGAA	30	2.1627184E-6	45.000004	4
TACGCGG	30	2.1627184E-6	45.000004	2
TACGCGC	30	2.1627184E-6	45.000004	34
TGCGCCC	20	7.0289726E-4	45.000004	45
TCTACGG	20	7.0289726E-4	45.000004	2
TATCTAG	20	7.0289726E-4	45.000004	19
ATAAGCG	20	7.0289726E-4	45.000004	33
CGTAAGC	65	0.0	45.000004	43
ATTATCG	30	2.1627184E-6	45.000004	30
GCTCGAA	115	0.0	45.000004	42
TAGTAAG	20	7.0289726E-4	45.000004	1
CGGGTCA	35	1.2098462E-7	45.0	6
CGCATGT	25	3.887231E-5	45.0	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content