##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935954.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466622
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.928035969157047	33.0	31.0	34.0	30.0	34.0
2	32.052625036967825	33.0	31.0	34.0	30.0	34.0
3	32.14103064150426	33.0	31.0	34.0	30.0	34.0
4	35.76294945373343	37.0	35.0	37.0	35.0	37.0
5	35.73747701565721	37.0	35.0	37.0	35.0	37.0
6	35.727876096712116	37.0	35.0	37.0	35.0	37.0
7	35.84796473376738	37.0	35.0	37.0	35.0	37.0
8	35.066587087621244	37.0	35.0	37.0	32.0	37.0
9	37.04162469836399	39.0	37.0	39.0	33.0	39.0
10	36.959794008855134	39.0	37.0	39.0	33.0	39.0
11	36.99087484087762	39.0	37.0	39.0	33.0	39.0
12	36.92228399003905	39.0	37.0	39.0	33.0	39.0
13	36.76147502689543	39.0	35.0	39.0	32.0	39.0
14	37.980956748717375	40.0	37.0	41.0	33.0	41.0
15	37.97242736090455	40.0	37.0	41.0	33.0	41.0
16	38.29743775475653	40.0	37.0	41.0	34.0	41.0
17	38.053514407807604	40.0	37.0	41.0	33.0	41.0
18	38.124535491254164	40.0	37.0	41.0	33.0	41.0
19	38.16352422303277	40.0	37.0	41.0	34.0	41.0
20	37.62845729519825	40.0	36.0	41.0	32.0	41.0
21	37.91916583444415	40.0	37.0	41.0	33.0	41.0
22	37.83748515929382	40.0	37.0	41.0	33.0	41.0
23	37.636718800228024	40.0	36.0	41.0	32.0	41.0
24	37.65312608492527	40.0	36.0	41.0	32.0	41.0
25	37.8108468953457	40.0	36.0	41.0	33.0	41.0
26	37.49596461375589	40.0	36.0	41.0	32.0	41.0
27	37.28948485069285	40.0	36.0	41.0	31.0	41.0
28	37.32906721071874	40.0	36.0	41.0	31.0	41.0
29	37.31934842334909	40.0	36.0	41.0	32.0	41.0
30	37.30989966182477	40.0	36.0	41.0	32.0	41.0
31	37.45567718624497	40.0	36.0	41.0	32.0	41.0
32	37.01308339512496	40.0	36.0	41.0	31.0	41.0
33	36.89358410019245	40.0	35.0	41.0	30.0	41.0
34	36.83647792002949	40.0	36.0	41.0	31.0	41.0
35	36.7442448062886	40.0	35.0	41.0	30.0	41.0
36	36.59822940195704	40.0	35.0	41.0	30.0	41.0
37	36.43837624458341	40.0	35.0	41.0	30.0	41.0
38	36.54762098658014	39.0	35.0	41.0	30.0	41.0
39	36.443980352405156	39.0	35.0	41.0	30.0	41.0
40	36.31908268362829	39.0	35.0	41.0	30.0	41.0
41	36.35521257034602	39.0	35.0	41.0	29.0	41.0
42	36.263697382463754	39.0	35.0	41.0	30.0	41.0
43	36.06349679183579	39.0	35.0	41.0	29.0	41.0
44	36.26135930153315	39.0	35.0	41.0	30.0	41.0
45	36.279980369549655	39.0	35.0	41.0	30.0	41.0
46	36.21749938922726	39.0	35.0	41.0	29.0	41.0
47	36.05279005276219	39.0	35.0	41.0	29.0	41.0
48	36.01817316800322	39.0	35.0	41.0	29.0	41.0
49	36.07728525444578	39.0	35.0	41.0	29.0	41.0
50	35.83744444111079	39.0	35.0	41.0	28.0	41.0
51	35.06926805851417	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	9.0
10	6.0
11	3.0
12	9.0
13	3.0
14	8.0
15	11.0
16	26.0
17	47.0
18	103.0
19	231.0
20	673.0
21	1438.0
22	2902.0
23	4272.0
24	4764.0
25	4793.0
26	4769.0
27	4647.0
28	4589.0
29	5031.0
30	6494.0
31	8697.0
32	11887.0
33	15901.0
34	25725.0
35	33843.0
36	32550.0
37	48037.0
38	85347.0
39	159643.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.40801762454406	17.234292425132118	18.679359310105394	8.678330640218421
2	26.472819541298954	38.33874099378083	19.359781579093998	15.828657885826214
3	27.009013719884617	37.92834457012314	21.038013638448252	14.02462807154399
4	24.728581164197145	19.94612341466969	38.785140863482646	16.540154557650517
5	36.27925815756651	23.846068123663265	19.920192361268864	19.95448135750136
6	22.648310624016872	42.17482244729138	22.241986018661784	12.93488091002996
7	65.61499457805246	5.501883751730523	23.20893571241819	5.674185957798818
8	61.85842073455602	19.874545135034353	11.196857413495291	7.070176716914334
9	57.21569064467599	7.779316020247652	11.592895319980627	23.41209801509573
10	42.55307293698111	21.932527827663506	20.014272794681776	15.500126440673606
11	34.901697733925964	21.26517823848854	24.13923904145111	19.69388498613439
12	26.76256155946355	18.819944194658632	27.27303899087484	27.14445525500298
13	27.06494764498888	21.320683551139894	34.03804364131995	17.576325162551274
14	19.028678459223954	32.82721346185992	24.78708676401884	23.357021314897285
15	14.89749733188748	23.151287337502303	38.721920526678986	23.229294803931232
16	16.544012069726673	24.33190033903245	27.78523087209776	31.338856719143116
17	16.5215099159491	28.92469707814891	30.585784639386915	23.968008366515082
18	16.686525710317987	25.203698068243675	29.206724072161194	28.903052149277148
19	18.70593328218558	26.620690837551592	26.14043058407019	28.532945296192636
20	25.75553660136042	24.79394456326534	32.54690091765926	16.903617917714982
21	20.721911954429924	33.99218210885899	26.391383175246773	18.894522761464312
22	17.93678823544539	22.873117855566175	31.55937782616336	27.630716082825067
23	22.379142003591774	29.651409492051386	27.68793584528805	20.281512659068795
24	22.084256635992304	25.47608128206557	25.823685981372503	26.615976100569625
25	17.59475549802624	37.285640196990286	23.276870786203823	21.842733518779657
26	17.515676500465045	24.589925035681986	32.3388095717733	25.555588892079673
27	25.26949007976478	29.290946419157258	23.513036247755142	21.926527253322817
28	15.742935395244972	29.03249311005482	31.159482407601867	24.065089087098336
29	20.467959076082998	23.727342474208246	32.094714779843216	23.709983669865544
30	17.3020131926913	36.17446241283094	26.322376570328878	20.201147824148883
31	20.91564478314353	24.95960327631359	21.707506289887746	32.41724565065513
32	25.859046508737265	33.21382189438132	22.059825726176648	18.86730587070477
33	21.384118194170014	28.670315587349076	22.05853988881793	27.887026329662984
34	20.667478172910837	24.21938957014457	26.47367676620477	28.639455490739827
35	24.963460788389746	27.21239032878861	23.38380959320392	24.44033928961772
36	17.61061415878377	28.293993853697426	29.05628110119111	25.03911088632769
37	18.18709790794262	32.2423717698694	27.388121434480155	22.182408887707826
38	18.409547771000938	28.4645816099541	22.676384739682227	30.449485879362737
39	20.933003587486233	28.799756548126748	25.522585733205894	24.744654131181125
40	22.562802439662082	26.57332916150546	27.739369339636795	23.124499059195667
41	17.49788908366944	24.836377196103054	27.763585943225994	29.902147777001513
42	23.209364324871096	23.11185499183493	31.05854417494246	22.620236508351514
43	22.106115871090516	24.86766590516521	28.303637633887814	24.722580589856456
44	20.773131142552216	24.686148531359432	28.50272811826275	26.037992207825607
45	19.37221134022828	21.395047811719124	26.462532842429205	32.770208005623395
46	26.173648049170424	28.046684468370543	24.489844027928388	21.289823454530648
47	16.96962423546254	25.792825884763253	36.47877725439435	20.758772625379855
48	20.694266451217473	25.5714475528372	25.978629383098095	27.75565661284723
49	19.617806275743536	21.215244887724968	34.994492329980154	24.17245650655134
50	21.252105558674902	22.02746548598223	30.005657684378363	26.714771270964505
51	19.215982101143965	21.18652785338025	26.612332894719927	32.98515715075585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	354.5
2	540.0
3	988.0
4	1436.0
5	1041.5
6	647.0
7	647.0
8	647.0
9	640.0
10	633.0
11	630.5
12	628.0
13	594.0
14	560.0
15	631.0
16	702.0
17	759.5
18	817.0
19	810.5
20	804.0
21	1017.0
22	1230.0
23	1216.5
24	1203.0
25	1779.0
26	2956.5
27	3558.0
28	4112.5
29	4667.0
30	5333.5
31	6000.0
32	7455.0
33	8910.0
34	9656.0
35	10402.0
36	11076.5
37	11751.0
38	13149.5
39	14548.0
40	16584.5
41	18621.0
42	21700.5
43	24780.0
44	56857.5
45	88935.0
46	68733.5
47	48532.0
48	48246.5
49	47961.0
50	40179.5
51	32398.0
52	29894.0
53	27390.0
54	24331.0
55	21272.0
56	19931.5
57	18591.0
58	17508.0
59	16425.0
60	16114.0
61	15803.0
62	14622.5
63	13442.0
64	10603.5
65	7765.0
66	6197.5
67	4630.0
68	3872.0
69	3114.0
70	2502.0
71	1890.0
72	1608.5
73	1327.0
74	1121.5
75	614.5
76	313.0
77	233.0
78	153.0
79	133.0
80	113.0
81	69.5
82	26.0
83	16.0
84	6.0
85	5.0
86	4.0
87	4.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	466622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.936120305336242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.6582688506755	20.735122883352748
2	11.30745173748221	6.543875676451371
3	4.177522888870796	3.626439146719835
4	2.0850878161318156	2.413374075791241
5	1.2571038975870348	1.8187854808442765
6	0.8789133763648961	1.5259405917878344
7	0.6688613165914836	1.3547976067133733
8	0.5550988922628485	1.2849926662301332
9	0.5102211710776158	1.328743906976829
>10	6.715469057336317	39.125434632452645
>50	0.11204879254187029	2.215326365901064
>100	0.05751838017149342	2.8525380086639025
>500	0.008963903403349623	1.7858353434554188
>1k	0.005228943651953947	3.9977033015260197
>5k	0.0	0.0
>10k+	0.002240975850837406	9.391090313133333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC	16054	3.4404721594781216	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG	14185	3.039933822237271	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	13208	2.8305566389925896	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	4474	0.9588060571511845	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACAACGTT	3371	0.7224262893734115	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	3053	0.6542769093613245	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTA	2313	0.49569030178602813	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTC	2098	0.44961446309861086	No Hit
GAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	2012	0.43118412762364394	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	1174	0.25159550985594337	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	946	0.20273369022463578	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	790	0.16930191889795165	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACAACGATCGAATGC	751	0.16094397606628064	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATG	731	0.15665785153721856	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACAACGT	716	0.153443258140422	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	709	0.15194311455525028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	673	0.14422809040293857	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	654	0.1401562721003296	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACAACGTTCGT	638	0.13672737247707997	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTAT	597	0.1279408171925027	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACACCGATCGAATGC	537	0.11508244360531651	No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	520	0.11143923775561375	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1430622645310336E-4	0.0	0.0	0.21280608286793165	0.0
2	2.1430622645310336E-4	0.0	0.0	1.6756603846368152	0.0
3	2.1430622645310336E-4	0.0	0.0	2.1302038909438474	0.0
4	2.1430622645310336E-4	0.0	0.0	3.0853667422453293	0.0
5	2.1430622645310336E-4	0.0	0.0	7.460642661511888	0.0
6	2.1430622645310336E-4	0.0	0.0	8.344227233178033	0.0
7	2.1430622645310336E-4	0.0	0.0	9.770649476449888	0.0
8	2.1430622645310336E-4	0.0	0.0	11.311939857100608	0.0
9	2.1430622645310336E-4	0.0	0.0	11.703905945283335	0.0
10	2.1430622645310336E-4	0.0	0.0	15.807012956954452	0.0
11	2.1430622645310336E-4	0.0	0.0	17.228934769470793	0.0
12	2.1430622645310336E-4	0.0	0.0	22.01032098786598	0.0
13	2.1430622645310336E-4	0.0	0.0	22.641024212317465	0.0
14	2.1430622645310336E-4	0.0	0.0	23.000844366532224	0.0
15	2.1430622645310336E-4	0.0	0.0	23.9176464032986	0.0
16	2.1430622645310336E-4	0.0	0.0	24.839591789499853	0.0
17	2.1430622645310336E-4	0.0	0.0	25.779538898723164	0.0
18	2.1430622645310336E-4	0.0	0.0	26.647050503405325	0.0
19	2.1430622645310336E-4	0.0	0.0	28.055685329881573	0.0
20	2.1430622645310336E-4	0.0	0.0	28.789469849257	0.0
21	2.1430622645310336E-4	0.0	0.0	29.45039025163837	0.0
22	4.286124529062067E-4	0.0	0.0	30.330545923681267	0.0
23	4.286124529062067E-4	0.0	0.0	31.066044892868316	0.0
24	4.286124529062067E-4	0.0	0.0	31.681960987694538	0.0
25	6.429186793593102E-4	0.0	0.0	32.19115258174711	0.0
26	6.429186793593102E-4	0.0	0.0	32.652125274847734	0.0
27	6.429186793593102E-4	0.0	0.0	33.23782419174407	0.0
28	6.429186793593102E-4	0.0	0.0	33.698582578618236	0.0
29	6.429186793593102E-4	0.0	0.0	34.19791608625397	0.0
30	6.429186793593102E-4	0.0	0.0	34.75875548088174	0.0
31	6.429186793593102E-4	0.0	0.0	35.28166267342731	0.0
32	6.429186793593102E-4	0.0	0.0	35.78228201842176	0.0
33	6.429186793593102E-4	0.0	0.0	36.244540548881105	0.0
34	8.572249058124134E-4	0.0	0.0	36.70079850499977	0.0
35	0.0010715311322655168	0.0	0.0	37.18727363904831	0.0
36	0.0010715311322655168	0.0	0.0	37.68917882140148	0.0
37	0.0010715311322655168	0.0	0.0	38.17329658695904	0.0
38	0.0010715311322655168	0.0	0.0	38.62119660024602	0.0
39	0.0010715311322655168	0.0	0.0	39.057095464851635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	20	7.0289726E-4	45.000004	35
GATCGAG	30	2.1627184E-6	45.000004	1
GAAGACG	20	7.0289726E-4	45.000004	1
CGTGCGT	20	7.0289726E-4	45.000004	11
GCGAAAT	20	7.0289726E-4	45.000004	22
ATAGCGG	20	7.0289726E-4	45.000004	2
CGACAGG	30	2.1627184E-6	45.000004	2
AGCGGAA	30	2.1627184E-6	45.000004	4
TACGCGG	30	2.1627184E-6	45.000004	2
TACGCGC	30	2.1627184E-6	45.000004	34
TGCGCCC	20	7.0289726E-4	45.000004	45
TCTACGG	20	7.0289726E-4	45.000004	2
TATCTAG	20	7.0289726E-4	45.000004	19
ATAAGCG	20	7.0289726E-4	45.000004	33
CGTAAGC	65	0.0	45.000004	43
ATTATCG	30	2.1627184E-6	45.000004	30
GCTCGAA	115	0.0	45.000004	42
TAGTAAG	20	7.0289726E-4	45.000004	1
CGGGTCA	35	1.2098462E-7	45.0	6
CGCATGT	25	3.887231E-5	45.0	38
>>END_MODULE