##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935953.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449439
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.060043743422355	33.0	31.0	34.0	30.0	34.0
2	32.18662376874281	33.0	31.0	34.0	30.0	34.0
3	32.23114148972385	34.0	31.0	34.0	30.0	34.0
4	35.79694241042722	37.0	35.0	37.0	35.0	37.0
5	35.821909091111365	37.0	35.0	37.0	35.0	37.0
6	35.8398136343308	37.0	35.0	37.0	35.0	37.0
7	36.01312970169478	37.0	35.0	37.0	35.0	37.0
8	35.703979850435765	37.0	35.0	37.0	35.0	37.0
9	37.44474333558058	39.0	37.0	39.0	34.0	39.0
10	37.16576665576419	39.0	37.0	39.0	34.0	39.0
11	37.09206143659095	39.0	37.0	39.0	34.0	39.0
12	36.905560042630924	39.0	35.0	39.0	33.0	39.0
13	36.82204036587835	39.0	35.0	39.0	33.0	39.0
14	37.71070823849288	40.0	37.0	41.0	33.0	41.0
15	37.91649367322373	40.0	37.0	41.0	33.0	41.0
16	38.12525837766638	40.0	37.0	41.0	33.0	41.0
17	38.14197477299478	40.0	37.0	41.0	33.0	41.0
18	38.13140826675033	40.0	37.0	41.0	34.0	41.0
19	38.13884642854759	40.0	37.0	41.0	34.0	41.0
20	37.95716882602533	40.0	36.0	41.0	33.0	41.0
21	37.923444560885905	40.0	36.0	41.0	33.0	41.0
22	37.96262896633358	40.0	36.0	41.0	34.0	41.0
23	37.98208210680426	40.0	36.0	41.0	34.0	41.0
24	37.914282027149405	40.0	36.0	41.0	34.0	41.0
25	37.83088917517171	40.0	36.0	41.0	33.0	41.0
26	37.69489741655708	40.0	35.0	41.0	33.0	41.0
27	37.61545838256137	40.0	35.0	41.0	33.0	41.0
28	37.60169678198821	40.0	35.0	41.0	33.0	41.0
29	37.555120939660334	40.0	35.0	41.0	33.0	41.0
30	37.42189485113664	39.0	35.0	41.0	33.0	41.0
31	37.42418214707669	40.0	35.0	41.0	33.0	41.0
32	37.38282392048754	40.0	35.0	41.0	33.0	41.0
33	37.34250699205009	40.0	35.0	41.0	33.0	41.0
34	37.24629593782471	40.0	35.0	41.0	32.0	41.0
35	37.20905172893318	40.0	35.0	41.0	32.0	41.0
36	37.11346812359408	39.0	35.0	41.0	32.0	41.0
37	37.05573392607228	39.0	35.0	41.0	32.0	41.0
38	36.99954165081357	39.0	35.0	41.0	32.0	41.0
39	36.93413121691709	39.0	35.0	41.0	31.0	41.0
40	36.83076457539288	39.0	35.0	41.0	31.0	41.0
41	36.69746951199161	39.0	35.0	41.0	31.0	41.0
42	36.724774663524975	39.0	35.0	41.0	31.0	41.0
43	36.67807867141036	39.0	35.0	41.0	31.0	41.0
44	36.602773680076716	39.0	35.0	41.0	31.0	41.0
45	36.48392106604011	39.0	35.0	41.0	31.0	41.0
46	36.446843731852375	39.0	35.0	41.0	31.0	41.0
47	36.389000509524095	38.0	35.0	41.0	31.0	41.0
48	36.3191423085224	38.0	35.0	41.0	31.0	41.0
49	36.288108063608185	38.0	35.0	40.0	31.0	41.0
50	36.15242780444065	38.0	35.0	40.0	31.0	41.0
51	35.147610687991026	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	15.0
11	11.0
12	8.0
13	10.0
14	21.0
15	21.0
16	36.0
17	58.0
18	117.0
19	206.0
20	350.0
21	617.0
22	977.0
23	1372.0
24	1875.0
25	2511.0
26	3133.0
27	3743.0
28	4425.0
29	5512.0
30	6652.0
31	8730.0
32	11716.0
33	17614.0
34	33294.0
35	34429.0
36	31595.0
37	47493.0
38	86444.0
39	146316.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22735899643778	21.276302234563534	23.47949332389935	11.016845445099335
2	32.712781934812064	24.78267351075452	23.941847503220682	18.562697051212734
3	32.31673263779957	25.56297962571116	25.017410594096194	17.102877142393073
4	29.17592821272742	24.425339145023017	26.507712948809512	19.891019693440043
5	26.667467665244892	28.075890165294958	24.348799280881277	20.907842888578873
6	26.226473448009628	34.62205104585939	23.77630779705366	15.375167709077317
7	79.11596456916288	5.553590142377497	10.14976448416804	5.180680804291573
8	79.32177670384635	6.203288989162044	8.776719421323028	5.698214885668578
9	72.82167324153	7.136007333586983	10.972568023691757	9.069751401191263
10	43.04900108802307	24.059327294694054	18.254757597805263	14.636914019477615
11	32.52009727682733	24.416661660425554	23.426315918289244	19.636925144457866
12	28.703116551968122	21.557096736153294	29.01639599589711	20.72339071598148
13	27.72567578692548	22.69540471565663	30.08617409704098	19.492745400376915
14	21.079612583687666	26.533967902206975	31.523521545749254	20.862897968356105
15	19.073778644042907	24.74128858421276	35.25528492186926	20.929647849875067
16	24.828285929792475	24.917953270632946	29.011946003795842	21.241814795778737
17	24.928633251675976	25.01696559488607	26.674365153001855	23.3800360004361
18	26.620520248576558	24.061107291534558	28.38271712067711	20.935655339211774
19	25.162480336597405	25.327575043554297	26.695947614692983	22.813997005155315
20	25.15469285042019	28.044072721770917	27.642683434236904	19.15855099357199
21	23.65949550439548	26.288773337427322	30.871152703703952	19.180578454473242
22	22.11290074960117	23.557590685276534	31.155507198974725	23.174001366147575
23	21.776258847140546	25.422582374916285	30.371418590732002	22.429740187211166
24	22.27688295853275	25.967483907716066	28.664401620687123	23.091231513064063
25	23.389380983848753	27.380578899472454	26.242715919179243	22.98732419749955
26	21.858138701803803	26.025111305427433	26.837234863908115	25.27951512886065
27	22.06083584201638	25.23256771219231	29.857222003430945	22.849374442360364
28	19.417095534655427	26.13079861783245	29.242678094246383	25.20942775326574
29	23.726690385124567	25.802834199969293	28.607219222185883	21.863256192720257
30	24.163679609468694	23.80634524373719	29.993391761729622	22.036583385064493
31	23.995247408435848	24.0457548187852	28.939856131755366	23.019141641023587
32	27.183221749781396	24.251789453073723	26.318588284505793	22.246400512639088
33	24.574413880415364	22.314040392578303	28.55337431776059	24.558171409245748
34	22.03925338032525	22.92546930729198	30.52049332612435	24.514783986258422
35	22.607739871261728	24.737061091716562	27.893217989538066	24.76198104748364
36	23.830375201084017	25.091280462977178	27.43753879836863	23.64080553757017
37	21.924221084507575	25.09283796021262	29.143443270388197	23.839497684891608
38	22.7959745371452	24.67364870427355	27.660928401852086	24.86944835672917
39	23.203593813620984	22.061725840436633	28.121057585122788	26.6136227608196
40	24.227091996911707	21.49635434397104	29.315435465102052	24.961118194015206
41	20.479531148832212	22.81978199488696	26.742672531756256	29.958014324524573
42	21.90241612321138	22.230158041469476	29.76510716693478	26.10231866838436
43	22.358095314380815	21.969611003940468	29.800484604139825	25.871809077538888
44	23.176003862593145	22.114680746441675	29.261145561466627	25.44816982949855
45	22.49738006715038	21.585131686391257	27.914800451229198	28.002687795229164
46	23.47749082745378	22.583709913914905	28.361357158591044	25.577442100040273
47	19.723032491617325	23.33887357349941	32.303382661495775	24.634711273387488
48	21.34127211924199	24.241554471240814	29.15924074234768	25.257932667169513
49	23.323966099960174	21.382212046573617	30.886950175663436	24.406871677802773
50	20.9874977471915	22.735232144962943	29.66854233833735	26.60872776950821
51	21.275189736538216	21.72441643916082	27.268884097730727	29.731509726570238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	181.0
1	386.0
2	591.0
3	901.0
4	1211.0
5	870.5
6	530.0
7	576.5
8	623.0
9	628.5
10	634.0
11	649.0
12	664.0
13	669.5
14	675.0
15	694.5
16	714.0
17	783.5
18	853.0
19	905.5
20	958.0
21	1126.5
22	1295.0
23	1372.0
24	1449.0
25	1694.5
26	2406.5
27	2873.0
28	3636.5
29	4400.0
30	5129.0
31	5858.0
32	6509.0
33	7160.0
34	8486.0
35	9812.0
36	10576.5
37	11341.0
38	12803.5
39	14266.0
40	15514.5
41	16763.0
42	18526.5
43	20290.0
44	22764.0
45	25238.0
46	27395.5
47	29553.0
48	36785.5
49	44018.0
50	46655.0
51	49292.0
52	49117.0
53	48942.0
54	42805.5
55	36669.0
56	32525.0
57	28381.0
58	26206.0
59	24031.0
60	20678.0
61	17325.0
62	15084.5
63	12844.0
64	11399.0
65	9954.0
66	8229.0
67	6504.0
68	5353.0
69	4202.0
70	3646.0
71	3090.0
72	2510.0
73	1930.0
74	1541.0
75	937.0
76	722.0
77	505.0
78	288.0
79	210.0
80	132.0
81	80.0
82	28.0
83	36.0
84	44.0
85	27.0
86	10.0
87	7.0
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	449439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.083132420919746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.50401814553659	24.370809190821717
2	11.316694928352284	7.714168236203635
3	4.186399241404856	4.28056799134919
4	2.089775697218752	2.849044072733064
5	1.3192121685379394	2.2481441515783644
6	0.9362809975557196	1.9146833533729455
7	0.7055080100134823	1.6832146050516361
8	0.589970348440196	1.608643000824269
9	0.5221790566526293	1.6017748141790265
>10	6.7006500666278095	44.99674558754789
>50	0.08576772528455318	2.005562522540535
>100	0.037605848778611776	2.3999740781561982
>500	0.001979255198874304	0.4411832794286949
>1k	0.003958510397748608	1.8854851162128476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1567	0.348656881134036	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC	1563	0.3477668827137832	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	1546	0.3439843894277088	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG	1369	0.3046019593315222	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1329	0.2957019751289941	TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1011	0.22494710071889623	No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	835	0.18578717022777286	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	575	0.1279372729113406	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	552	0.12281978199488695	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.22499605063201E-4	0.0	0.0	0.1935746564049849	0.0
2	2.22499605063201E-4	0.0	0.0	1.0501981358983088	0.0
3	2.22499605063201E-4	0.0	0.0	1.471167388677885	0.0
4	2.22499605063201E-4	0.0	0.0	2.0621263397257468	0.0
5	2.22499605063201E-4	0.0	0.0	3.8915180925553856	0.0
6	2.22499605063201E-4	0.0	0.0	4.782629010833506	0.0
7	2.22499605063201E-4	0.0	0.0	5.664394945698971	0.0
8	2.22499605063201E-4	0.0	0.0	6.867895309485825	0.0
9	2.22499605063201E-4	0.0	0.0	7.324686998680577	0.0
10	2.22499605063201E-4	0.0	0.0	8.657459633009152	0.0
11	2.22499605063201E-4	0.0	0.0	10.384501567509718	0.0
12	2.22499605063201E-4	0.0	0.0	12.046573617331829	0.0
13	2.22499605063201E-4	0.0	0.0	12.65555503638981	0.0
14	2.22499605063201E-4	0.0	0.0	12.936127038374506	0.0
15	2.22499605063201E-4	0.0	0.0	13.415613687285704	0.0
16	2.22499605063201E-4	0.0	0.0	14.319629582657491	0.0
17	2.22499605063201E-4	0.0	0.0	15.283275372186214	0.0
18	2.22499605063201E-4	0.0	0.0	16.28875108746682	0.0
19	2.22499605063201E-4	0.0	0.0	17.013209801552602	0.0
20	2.22499605063201E-4	0.0	0.0	17.69361359383587	0.0
21	4.44999210126402E-4	0.0	0.0	18.50684965034187	0.0
22	4.44999210126402E-4	0.0	0.0	19.353238148002287	0.0
23	4.44999210126402E-4	0.0	0.0	20.084371850239965	0.0
24	4.44999210126402E-4	0.0	0.0	20.700028257449844	0.0
25	4.44999210126402E-4	0.0	0.0	21.223124828953427	0.0
26	4.44999210126402E-4	0.0	0.0	21.787828826603832	0.0
27	4.44999210126402E-4	0.0	0.0	22.483807591241526	0.0
28	4.44999210126402E-4	0.0	0.0	23.054519078228637	0.0
29	4.44999210126402E-4	0.0	0.0	23.6666154917575	0.0
30	4.44999210126402E-4	0.0	0.0	24.39418920031417	0.0
31	4.44999210126402E-4	0.0	0.0	24.96490068730128	0.0
32	6.674988151896031E-4	0.0	0.0	25.48554976314917	0.0
33	0.0011124980253160051	0.0	0.0	26.019103816090727	0.0
34	0.0011124980253160051	0.0	0.0	26.618962751341115	0.0
35	0.0011124980253160051	0.0	0.0	27.23083666526492	0.0
36	0.0011124980253160051	0.0	0.0	27.7563807324242	0.0
37	0.0011124980253160051	0.0	0.0	28.332877209142953	0.0
38	0.0011124980253160051	0.0	0.0	28.974121070935098	0.0
39	0.0013349976303792062	0.0	0.0	30.096409078873887	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.0286973E-4	45.0	41
CGAAATA	20	7.0286973E-4	45.0	40
GGCGTAG	20	7.0286973E-4	45.0	1
ATACTCC	25	3.8870017E-5	45.0	28
GCCGATT	35	1.2097189E-7	45.0	9
TTCCCGA	20	7.0286973E-4	45.0	20
TGACCGT	25	3.8870017E-5	45.0	20
TCGTAGA	20	7.0286973E-4	45.0	12
CGTCATA	35	1.2097189E-7	45.0	38
TAATGCG	25	3.8870017E-5	45.0	1
AGTACGG	20	7.0286973E-4	45.0	2
GCGTACG	20	7.0286973E-4	45.0	1
TACCGGT	20	7.0286973E-4	45.0	40
CGCCAAT	20	7.0286973E-4	45.0	14
CCCGATC	20	7.0286973E-4	45.0	41
TAATCTA	20	7.0286973E-4	45.0	17
CTAGCGG	35	1.2097189E-7	45.0	2
AGGGCGT	30	2.16254E-6	44.999996	6
AATGCGG	80	0.0	42.1875	2
ACGGGCC	55	6.002665E-11	40.909092	5
>>END_MODULE