SRR2935951.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935951.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	707666
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3676	0.5194540927499696	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1658	0.23429131822074256	TruSeq Adapter, Index 14 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1366	0.19302891477052733	Illumina PCR Primer Index 3 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1263	0.1784740258822665	TruSeq Adapter, Index 14 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1229	0.17366949945313184	Illumina PCR Primer Index 3 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	821	0.11601518230351607	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	765	0.10810184465552958	TruSeq Adapter, Index 14 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	710	0.1003298166084	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGG	60	0.0	45.000004	2
GTTTACG	20	7.0314476E-4	45.0	1
CCGTGAC	20	7.0314476E-4	45.0	28
TAACGCG	20	7.0314476E-4	45.0	1
ACGTGCG	40	6.8084773E-9	45.0	1
TCGAGCG	35	1.2110468E-7	45.0	1
ATCGGCG	20	7.0314476E-4	45.0	31
TTATTCG	20	7.0314476E-4	45.0	11
CGATAGT	25	3.889281E-5	45.0	12
CGTTTTT	1865	0.0	43.19035	1
TAGCGAG	55	6.002665E-11	40.90909	1
TCGATAG	50	1.0804797E-9	40.5	1
CGTAAGG	80	0.0	39.375	2
GTGAGCG	40	3.455716E-7	39.375	1
CTACGAA	105	0.0	38.57143	11
CGAATAT	105	0.0	38.57143	14
GGGCGAT	1005	0.0	37.83582	7
ACGTTAG	30	1.13947826E-4	37.500004	1
CTCGCGG	30	1.13947826E-4	37.500004	2
TATCGCG	30	1.13947826E-4	37.500004	1