##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935950.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 537111 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06137837430252 33.0 31.0 34.0 30.0 34.0 2 32.178262966128045 33.0 31.0 34.0 30.0 34.0 3 32.23456231579692 34.0 31.0 34.0 30.0 34.0 4 35.80746437887141 37.0 35.0 37.0 35.0 37.0 5 35.822494791579395 37.0 35.0 37.0 35.0 37.0 6 35.83219483495963 37.0 35.0 37.0 35.0 37.0 7 36.00099793152626 37.0 35.0 37.0 35.0 37.0 8 35.682102954510334 37.0 35.0 37.0 35.0 37.0 9 37.43172826473485 39.0 37.0 39.0 34.0 39.0 10 37.193933842352884 39.0 37.0 39.0 34.0 39.0 11 37.11817296610943 39.0 37.0 39.0 34.0 39.0 12 36.876224839930664 39.0 35.0 39.0 33.0 39.0 13 36.77438183168842 39.0 35.0 39.0 33.0 39.0 14 37.63671196456598 40.0 36.0 41.0 33.0 41.0 15 37.86562740290182 40.0 36.0 41.0 33.0 41.0 16 38.064362859818544 40.0 36.0 41.0 33.0 41.0 17 38.067615446341634 40.0 36.0 41.0 33.0 41.0 18 38.04022073649581 40.0 36.0 41.0 33.0 41.0 19 38.072876928605076 40.0 36.0 41.0 34.0 41.0 20 37.91191206286969 40.0 36.0 41.0 33.0 41.0 21 37.91606762847903 40.0 36.0 41.0 33.0 41.0 22 37.937225266285736 40.0 36.0 41.0 34.0 41.0 23 37.96175278480612 40.0 36.0 41.0 34.0 41.0 24 37.87613174930322 40.0 35.0 41.0 34.0 41.0 25 37.805591395447124 40.0 35.0 41.0 33.0 41.0 26 37.64564866480113 40.0 35.0 41.0 33.0 41.0 27 37.58502246276841 40.0 35.0 41.0 33.0 41.0 28 37.583194162845295 40.0 35.0 41.0 33.0 41.0 29 37.580749602968474 40.0 35.0 41.0 33.0 41.0 30 37.43214717255837 39.0 35.0 41.0 33.0 41.0 31 37.45653133151248 40.0 35.0 41.0 33.0 41.0 32 37.42472040230046 40.0 35.0 41.0 33.0 41.0 33 37.38730727912852 40.0 35.0 41.0 33.0 41.0 34 37.32849820614361 40.0 35.0 41.0 33.0 41.0 35 37.28621085771842 40.0 35.0 41.0 32.0 41.0 36 37.19872614785398 40.0 35.0 41.0 32.0 41.0 37 37.16698224389372 39.0 35.0 41.0 32.0 41.0 38 37.11396713156126 39.0 35.0 41.0 32.0 41.0 39 37.08118805982376 39.0 35.0 41.0 32.0 41.0 40 36.97676085576352 39.0 35.0 41.0 32.0 41.0 41 36.854300135353775 39.0 35.0 41.0 32.0 41.0 42 36.866979078812385 39.0 35.0 41.0 32.0 41.0 43 36.82526330683974 39.0 35.0 41.0 32.0 41.0 44 36.77721923401308 39.0 35.0 41.0 32.0 41.0 45 36.68096724885545 39.0 35.0 41.0 31.0 41.0 46 36.63450757850798 39.0 35.0 41.0 31.0 41.0 47 36.56919147066435 39.0 35.0 41.0 31.0 41.0 48 36.52447631867528 38.0 35.0 41.0 31.0 41.0 49 36.508252484123396 38.0 35.0 41.0 31.0 41.0 50 36.37996056681021 38.0 35.0 41.0 31.0 41.0 51 35.430618624455654 37.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 10.0 9 17.0 10 17.0 11 16.0 12 15.0 13 14.0 14 14.0 15 28.0 16 50.0 17 78.0 18 136.0 19 224.0 20 427.0 21 737.0 22 976.0 23 1454.0 24 1910.0 25 2659.0 26 3437.0 27 4305.0 28 5036.0 29 6167.0 30 7805.0 31 10168.0 32 13807.0 33 20694.0 34 41754.0 35 44415.0 36 36742.0 37 55426.0 38 98492.0 39 179950.0 40 130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01511047064759 21.245701540277523 23.948494817644768 10.790693171430114 2 32.385484564643065 25.208197188290686 23.88928917858692 18.517029068479328 3 32.732340242519705 24.73101463198482 25.272243539975907 17.26440158551957 4 30.029919327662252 25.095929891586653 25.38693119299363 19.487219587757465 5 27.050460705515246 28.012273068322934 23.834738070901544 21.102528155260273 6 26.753501603951513 33.151620428552015 24.404266529637265 15.690611437859214 7 77.61300736719225 6.303538747111863 10.41926156790682 5.66419231778906 8 77.41155924939164 6.69135430106626 9.679004898428817 6.21808155111327 9 71.24653935592457 7.712930846696493 11.788810878943085 9.251718918435854 10 44.995354777690274 21.720091377759903 18.90223808486514 14.382315759684683 11 34.22532772555393 22.933434615936 23.220153748480296 19.62108391002977 12 31.17381695776106 20.300459309155837 28.293406763220265 20.23231696986284 13 28.29768893208294 20.95116279502747 30.677457732200608 20.073690540688983 14 20.861609611421102 25.48244217675676 31.15557119478097 22.50037701704117 15 20.1719942432756 23.995971037643987 35.27371437188961 20.558320347190804 16 25.597502192284278 24.53980648320366 28.488338537099406 21.374352787412658 17 25.260327939662382 23.890220084861415 27.10315000065163 23.746301974824572 18 24.955921587902687 24.589516878261662 29.250564594655483 21.20399693918017 19 25.617237405303563 25.890551487495134 26.48242169681872 22.00978941038258 20 24.988503307509994 28.489269443373903 27.09346857539689 19.428758673719212 21 25.118457823429424 26.15585977572606 29.625719823276754 19.099962577567766 22 22.521787861354543 23.920381448155037 29.58271195339511 23.97511873709531 23 22.595515638294504 23.92857342337059 30.67410646961243 22.80180446872248 24 24.737717157161182 24.508528032380646 27.441627522057825 23.31212728840035 25 24.212127474581603 26.048247010394498 25.801556847653462 23.938068667370434 26 21.58250343038962 25.494357777070288 27.22993943523778 25.69319935730231 27 22.053169642774026 26.065561867100094 29.09770978438349 22.783558705742387 28 19.715850168773308 25.924808838396533 28.499695593648237 25.859645399181918 29 23.52102265639691 26.035586685061375 27.98341497381361 22.45997568472811 30 24.752425476298196 26.458590496191665 27.487986654527646 21.300997372982494 31 24.033579651133564 25.307059434642003 28.584780427137034 22.0745804870874 32 26.630994338228035 25.559148853775106 26.788131317362705 21.021725490634154 33 25.243943989231276 25.96018327682732 25.86802355565237 22.92784917828903 34 22.58304149421628 23.46516827992724 31.522720629441586 22.429069596414895 35 24.033021107368867 26.040985941453442 27.76241782424862 22.163575126929068 36 24.35585940336355 27.68478024095578 26.030001247414408 21.92935910826626 37 21.55066643580191 27.530435980644597 29.814321434489333 21.10457614906416 38 23.241936955303466 26.190303307882356 27.07205773108352 23.49570200573066 39 22.58248295045158 24.176939217405714 28.248350899534735 24.992226932607974 40 25.05180493417562 23.36928493365431 29.223009768930446 22.355900363239627 41 21.9228427643448 24.43684824924457 26.679587645756648 26.960721340653983 42 22.39127480167042 23.325532338753067 29.303440071046765 24.979752788529748 43 22.487902872962945 22.96154798542573 29.09938541567758 25.451163725933746 44 21.824166699248387 23.027642330914837 28.8262575147409 26.321933455095873 45 22.40784493335642 22.487158147943347 27.64903344001519 27.455963478685042 46 23.65041862855164 23.968974755683647 27.891999977658248 24.48860663810646 47 20.303624390489116 23.878863028312583 32.27265872417433 23.544853857023966 48 20.97313218310554 23.757472850118504 30.287966546952124 24.981428419823835 49 23.038813206208772 21.790654073366586 31.776113317358984 23.394419403065662 50 21.159685800514232 22.136392663713835 30.120589598798013 26.58333193697392 51 20.677290169071195 22.24921850418256 27.83093252605141 29.24255880069483 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 217.0 1 486.0 2 755.0 3 1080.0 4 1405.0 5 1045.0 6 685.0 7 689.5 8 694.0 9 693.5 10 693.0 11 738.5 12 784.0 13 778.5 14 773.0 15 755.5 16 738.0 17 832.5 18 927.0 19 923.0 20 919.0 21 1082.5 22 1246.0 23 1344.5 24 1443.0 25 1809.0 26 2733.5 27 3292.0 28 3592.0 29 3892.0 30 4870.5 31 5849.0 32 6865.5 33 7882.0 34 9649.5 35 11417.0 36 12205.0 37 12993.0 38 14961.5 39 16930.0 40 19519.5 41 22109.0 42 25888.5 43 29668.0 44 38917.5 45 48167.0 46 52729.0 47 57291.0 48 57185.5 49 57080.0 50 49836.5 51 42593.0 52 38573.5 53 34554.0 54 32710.5 55 30867.0 56 29697.0 57 28527.0 58 28056.0 59 27585.0 60 25031.0 61 22477.0 62 20794.5 63 19112.0 64 16553.5 65 13995.0 66 11642.0 67 9289.0 68 7896.0 69 6503.0 70 5622.0 71 4741.0 72 3902.0 73 3063.0 74 2327.5 75 1291.5 76 991.0 77 818.0 78 645.0 79 456.0 80 267.0 81 210.0 82 153.0 83 107.0 84 61.0 85 61.0 86 61.0 87 34.0 88 7.0 89 4.0 90 1.0 91 2.0 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 537111.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.86788040974036 #Duplication Level Percentage of deduplicated Percentage of total 1 69.8004712270243 22.243930696063334 2 12.12360152181423 7.727069668650441 3 4.557684186352122 4.357312037881026 4 2.2524145351196108 2.871187081534107 5 1.3655401467651676 2.1758435045905826 6 0.9868604489315393 1.8869490460591782 7 0.7017537220698548 1.5654382584408613 8 0.6060261444107304 1.545021495620576 9 0.4924679579307848 1.4124518990070565 >10 6.93969492973763 46.130245814944516 >50 0.11624687220839912 2.451767058583039 >100 0.050157279886872665 2.905526370775158 >500 0.0029504282286395684 0.5667760327173856 >1k 0.004130599520095396 2.1604810351327286 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC 2154 0.40103442305221826 Illumina Single End Adapter 2 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC 1837 0.3420149652492688 Illumina Single End Adapter 2 (95% over 21bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC 1820 0.3388498839159876 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG 1705 0.3174390396026147 No Hit GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC 1676 0.31203978321054676 No Hit CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT 1215 0.22621022470215654 Illumina Single End Adapter 2 (95% over 22bp) GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 1082 0.2014481178006036 No Hit TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC 692 0.12883742839003484 Illumina Single End Adapter 2 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 631 0.11748037184120229 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 612 0.11394292799812328 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC 556 0.10351677772378523 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.8618125489889428E-4 0.0 0.0 0.23402983740791009 0.0 2 1.8618125489889428E-4 0.0 0.0 1.2368020762933547 0.0 3 1.8618125489889428E-4 0.0 0.0 1.7486143460104149 0.0 4 1.8618125489889428E-4 0.0 0.0 2.4157017823131532 0.0 5 1.8618125489889428E-4 0.0 0.0 4.516943425102074 0.0 6 1.8618125489889428E-4 0.0 0.0 5.440588630655489 0.0 7 1.8618125489889428E-4 0.0 0.0 6.480410939265813 0.0 8 1.8618125489889428E-4 0.0 0.0 7.90041537037968 0.0 9 1.8618125489889428E-4 0.0 0.0 8.402732396096896 0.0 10 1.8618125489889428E-4 0.0 0.0 9.804304882975773 0.0 11 1.8618125489889428E-4 0.0 0.0 11.656436006709972 0.0 12 1.8618125489889428E-4 0.0 0.0 13.33895600723128 0.0 13 3.7236250979778856E-4 0.0 0.0 13.918724434986437 0.0 14 3.7236250979778856E-4 0.0 0.0 14.18738398580554 0.0 15 3.7236250979778856E-4 0.0 0.0 14.70124424932649 0.0 16 3.7236250979778856E-4 0.0 0.0 15.630102530017073 0.0 17 3.7236250979778856E-4 0.0 0.0 16.636226031490697 0.0 18 3.7236250979778856E-4 0.0 0.0 17.682564684022484 0.0 19 3.7236250979778856E-4 0.0 0.0 18.458009610676378 0.0 20 3.7236250979778856E-4 0.0 0.0 19.19659064886029 0.0 21 3.7236250979778856E-4 0.0 0.0 20.0371990147288 0.0 22 3.7236250979778856E-4 0.0 0.0 20.911878550243806 0.0 23 3.7236250979778856E-4 0.0 0.0 21.706500146152287 0.0 24 3.7236250979778856E-4 0.0 0.0 22.36204434465129 0.0 25 3.7236250979778856E-4 0.0 0.0 22.984820642288092 0.0 26 3.7236250979778856E-4 0.0 0.0 23.562354894984463 0.0 27 3.7236250979778856E-4 0.0 0.0 24.21640964344428 0.0 28 3.7236250979778856E-4 0.0 0.0 24.801018783826805 0.0 29 5.585437646966828E-4 0.0 0.0 25.43868958185552 0.0 30 5.585437646966828E-4 0.0 0.0 26.15604595698096 0.0 31 5.585437646966828E-4 0.0 0.0 26.756480504029895 0.0 32 5.585437646966828E-4 0.0 0.0 27.30776319978552 0.0 33 5.585437646966828E-4 0.0 0.0 27.886973083775977 0.0 34 5.585437646966828E-4 0.0 0.0 28.49094507466799 0.0 35 5.585437646966828E-4 0.0 0.0 29.115397003598883 0.0 36 5.585437646966828E-4 0.0 0.0 29.687531999903186 0.0 37 5.585437646966828E-4 0.0 0.0 30.273816771579803 0.0 38 7.447250195955771E-4 0.0 0.0 30.938483851568858 0.0 39 7.447250195955771E-4 0.0 0.0 31.97700289139489 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGATC 45 3.8380676E-10 45.000004 26 AGGTACG 20 7.029927E-4 45.0 1 CGTATTA 20 7.029927E-4 45.0 18 CACGGGT 20 7.029927E-4 45.0 4 AACTTAG 20 7.029927E-4 45.0 1 GCTATCG 20 7.029927E-4 45.0 1 TAAACCG 20 7.029927E-4 45.0 1 TAAGTCG 25 3.8880215E-5 45.0 24 ATATGCG 25 3.8880215E-5 45.0 1 CCGTATT 20 7.029927E-4 45.0 17 CCCCGTA 20 7.029927E-4 45.0 15 TACGCGG 35 1.2103192E-7 45.0 2 CTACGCG 20 7.029927E-4 45.0 1 CGGGATC 85 0.0 45.0 6 ACTCGAG 25 3.8880215E-5 45.0 1 CATCGTT 25 3.8880215E-5 45.0 36 AGTTACG 25 3.8880215E-5 45.0 1 GGCATAG 55 1.8189894E-12 45.0 1 ACCGTTA 20 7.029927E-4 45.0 25 CAATGCG 25 3.8880215E-5 45.0 1 >>END_MODULE