##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935948.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425777
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16418923520998	33.0	31.0	34.0	30.0	34.0
2	32.27588855198848	33.0	31.0	34.0	30.0	34.0
3	32.32557888284243	34.0	31.0	34.0	30.0	34.0
4	35.868508632453135	37.0	35.0	37.0	35.0	37.0
5	35.908841952477474	37.0	35.0	37.0	35.0	37.0
6	35.93805207890515	37.0	35.0	37.0	35.0	37.0
7	36.12055136843935	37.0	35.0	37.0	35.0	37.0
8	35.87848568616905	37.0	35.0	37.0	35.0	37.0
9	37.65318229965452	39.0	38.0	39.0	35.0	39.0
10	37.36978512225883	39.0	37.0	39.0	34.0	39.0
11	37.25407666454505	39.0	37.0	39.0	34.0	39.0
12	37.277812094124386	39.0	37.0	39.0	34.0	39.0
13	37.346535862669896	39.0	37.0	39.0	34.0	39.0
14	38.545405223861316	40.0	38.0	41.0	34.0	41.0
15	38.68277760423884	40.0	38.0	41.0	35.0	41.0
16	38.778085711534445	40.0	38.0	41.0	35.0	41.0
17	38.75728139378125	40.0	38.0	41.0	35.0	41.0
18	38.72430873438443	40.0	38.0	41.0	35.0	41.0
19	38.750258938364446	40.0	38.0	41.0	35.0	41.0
20	38.62106454787365	40.0	38.0	41.0	35.0	41.0
21	38.612600023016746	40.0	38.0	41.0	35.0	41.0
22	38.58923802835757	40.0	38.0	41.0	34.0	41.0
23	38.6037456227086	40.0	38.0	41.0	35.0	41.0
24	38.54991932396536	40.0	38.0	41.0	34.0	41.0
25	38.48664676579524	40.0	38.0	41.0	34.0	41.0
26	38.347184089323754	40.0	38.0	41.0	34.0	41.0
27	38.31972605377933	40.0	38.0	41.0	34.0	41.0
28	38.30232727460619	40.0	38.0	41.0	34.0	41.0
29	38.30154987235102	40.0	38.0	41.0	34.0	41.0
30	38.20930909842476	40.0	37.0	41.0	34.0	41.0
31	38.15568008605444	40.0	37.0	41.0	34.0	41.0
32	38.12009338221651	40.0	37.0	41.0	34.0	41.0
33	38.0916630066913	40.0	37.0	41.0	34.0	41.0
34	37.95960326649866	40.0	37.0	41.0	33.0	41.0
35	37.97512547648182	40.0	37.0	41.0	33.0	41.0
36	37.91907031145412	40.0	37.0	41.0	33.0	41.0
37	37.85949687277613	40.0	37.0	41.0	33.0	41.0
38	37.86326410303985	40.0	37.0	41.0	33.0	41.0
39	37.76790197685643	40.0	37.0	41.0	33.0	41.0
40	37.64911913983141	40.0	37.0	41.0	33.0	41.0
41	37.54184937185428	40.0	36.0	41.0	33.0	41.0
42	37.63422167002915	40.0	37.0	41.0	33.0	41.0
43	37.63733127904983	40.0	37.0	41.0	33.0	41.0
44	37.603282939191175	40.0	36.0	41.0	33.0	41.0
45	37.48133412561035	40.0	36.0	41.0	33.0	41.0
46	37.39977030229439	40.0	36.0	41.0	33.0	41.0
47	37.337942162211675	40.0	36.0	41.0	32.0	41.0
48	37.30038024599732	39.0	36.0	41.0	32.0	41.0
49	37.26035459876884	39.0	36.0	41.0	32.0	41.0
50	37.150029240658846	39.0	35.0	41.0	32.0	41.0
51	36.099885620876655	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	11.0
10	10.0
11	7.0
12	9.0
13	6.0
14	6.0
15	17.0
16	15.0
17	44.0
18	79.0
19	116.0
20	191.0
21	367.0
22	523.0
23	766.0
24	1103.0
25	1520.0
26	2070.0
27	2416.0
28	2962.0
29	3633.0
30	4618.0
31	6062.0
32	8028.0
33	11397.0
34	20847.0
35	27686.0
36	27156.0
37	43207.0
38	88818.0
39	171919.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.112547178452566	22.452363561206923	25.33885108871545	12.096238171625053
2	30.318687951674235	25.610119851471545	26.25083083398117	17.82036136287305
3	31.08152859360651	25.59086094363951	26.957069076065636	16.370541386688338
4	28.89211958372575	25.410719695991098	27.4282077237615	18.268952996521655
5	26.75579000274792	29.755012600492748	24.452001869523247	19.03719552723609
6	25.92084588881034	34.165302494028566	23.883394359018922	16.03045725814217
7	81.19626001404491	5.073078160633384	8.57984343917121	5.150818386150497
8	81.8858228603236	5.014361978218645	7.590828062577358	5.508987098880399
9	75.65674050030886	7.749126890367493	9.570502868872673	7.023629740450987
10	35.46809714944678	37.62439023244562	15.71714770877713	11.190364909330471
11	25.053960171639144	25.551873398516122	30.57938780159567	18.81477862824906
12	25.574655277293047	22.479842734577023	32.80308706200664	19.1424149261233
13	24.587753683266122	22.94863273497629	32.87753918130852	19.58607440044906
14	17.794526242610566	29.33084689872868	30.482388668246525	22.392238190414233
15	16.337190595076763	29.348461753453098	36.331459895673085	17.982887755797048
16	19.499644179934567	27.963934172113568	33.328244597524055	19.208177050427803
17	19.34463345835966	26.934756926748037	30.224037465621674	23.496572149270627
18	19.46958149453822	28.314587213494384	31.1691331377693	21.04669815419809
19	21.94388142149529	28.418162559273984	29.37688038574183	20.261075633488893
20	24.69649605309822	27.920014467667347	29.958170591647743	17.425318887586695
21	23.609776948966243	26.67217815898933	30.365895762335683	19.352149129708746
22	20.0081263196462	26.61158305873732	29.3169898796788	24.06330074193768
23	19.915589616160574	28.144780013950964	30.302247420598107	21.637382949290355
24	21.852753906387616	25.327342716962164	30.05493486026723	22.76496851638299
25	20.158909476087246	28.47100712344725	27.69337000354646	23.676713396919045
26	19.462300687918795	29.084943526775753	29.414928471946585	22.03782731335887
27	21.610843234838896	28.069388435730442	29.15704699878105	21.16272133064961
28	19.33335995133603	28.311299107279165	30.596298062131115	21.75904287925369
29	21.528170849998943	25.866827000988778	31.352092762173626	21.25290938683865
30	23.380548972819103	24.98749345314566	28.74485939822959	22.887098175805644
31	22.37955549501265	27.464846621588297	27.622675719919116	22.53292216347994
32	25.496680187046273	27.59754519384561	27.027528494963327	19.8782461241448
33	24.545478031927512	27.667065153824655	26.63436493751424	21.153091876733594
34	23.63702125760668	25.313250833182625	30.266547981689946	20.783179927520745
35	23.22788689854079	24.116380170840603	29.012605190040798	23.643127740577814
36	21.944586015684266	27.873276386465218	28.66406593122691	21.51807166662361
37	23.19336178328092	27.084130894811132	29.743739093469117	19.97876822843883
38	25.18736333808543	25.74563680048476	26.082432822815697	22.98456703861411
39	25.822672431812897	24.98350075274146	28.259628866754195	20.934197948691452
40	24.475488342489143	22.869013591621908	32.225084962315954	20.430413103573
41	20.78928641049188	26.81638510299993	29.587788912975572	22.806539573532625
42	23.35330466417867	26.173090608464054	29.230559659164307	21.243045068192977
43	23.788743872966364	26.2489519161439	27.469543916181475	22.492760294708265
44	23.35001655796344	25.318887586694444	28.125286241389276	23.205809613952844
45	23.3857159968716	22.461288420933965	28.443058220617836	25.7099373615766
46	23.692449333806195	24.81721652414292	28.675809167709858	22.814524974341026
47	21.411677944088105	24.603019890693957	31.88734948106638	22.09795268415156
48	22.4347487064825	23.458289198336217	29.522261653400722	24.58470044178056
49	22.61911751926478	23.19758934841478	31.615376124121315	22.567917008199128
50	21.635738896182744	23.188194759228423	30.188338026713513	24.98772831787532
51	20.66739161579888	23.236811758267827	28.275599668371004	27.820196957562292
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	322.0
2	517.0
3	788.0
4	1059.0
5	784.0
6	509.0
7	497.5
8	486.0
9	519.0
10	552.0
11	560.0
12	568.0
13	594.0
14	620.0
15	638.5
16	657.0
17	739.0
18	821.0
19	939.5
20	1058.0
21	1231.5
22	1405.0
23	1904.0
24	2403.0
25	3067.0
26	4337.5
27	4944.0
28	5858.0
29	6772.0
30	7691.0
31	8610.0
32	10040.5
33	11471.0
34	12476.5
35	13482.0
36	15151.0
37	16820.0
38	17697.5
39	18575.0
40	21263.5
41	23952.0
42	24038.5
43	24125.0
44	25905.5
45	27686.0
46	29426.5
47	31167.0
48	35877.0
49	40587.0
50	43808.5
51	47030.0
52	44664.5
53	42299.0
54	34311.5
55	26324.0
56	22218.0
57	18112.0
58	16337.5
59	14563.0
60	14204.0
61	13845.0
62	12380.5
63	10916.0
64	8014.0
65	5112.0
66	3785.0
67	2458.0
68	1791.5
69	1125.0
70	907.5
71	690.0
72	476.0
73	262.0
74	212.0
75	133.5
76	105.0
77	64.0
78	23.0
79	22.0
80	21.0
81	20.5
82	20.0
83	12.5
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	425777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.99705354396151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0228887705321	23.805718988346417
2	11.890174864754382	8.0846182304824
3	4.2764871750011	4.3616389040573145
4	2.17683033865648	2.960232703176969
5	1.3488618014513243	2.292866344367253
6	0.9322257228075901	1.9015756688008718
7	0.8419032186993984	2.003556016346989
8	0.7007814521119698	1.905960364005259
9	0.6063150676707298	1.8551633238101204
>10	7.0905311343162944	44.839439034181446
>50	0.06766076566585948	1.5814926230262996
>100	0.03975941899952567	2.3545719379259453
>500	0.003487668333291726	0.8676985316619029
>1k	0.002092600999975035	1.185467329810837
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1972	0.4631532468874552	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1746	0.4100738179845318	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1281	0.3008617186931187	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	903	0.21208285088203446	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	787	0.18483854224159596	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	737	0.17309530575864832	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	698	0.16393558130194916	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	534	0.12541776563788087	No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	466	0.10944696402107207	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	445	0.10451480469823406	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCC	427	0.10028723956437291	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31565819666163275	0.0
2	0.0	0.0	0.0	1.516051829948541	0.0
3	0.0	0.0	0.0	2.119889049901709	0.0
4	0.0	0.0	0.0	2.8611221367053647	0.0
5	0.0	0.0	0.0	4.916658250680521	0.0
6	0.0	0.0	0.0	5.869974188366211	0.0
7	0.0	0.0	0.0	6.870732801443009	0.0
8	0.0	0.0	0.0	8.443152166509698	0.0
9	0.0	0.0	0.0	8.965961054730528	0.0
10	0.0	0.0	0.0	10.377732944710495	0.0
11	0.0	0.0	0.0	12.244202951310193	0.0
12	0.0	0.0	0.0	14.020250036991195	0.0
13	0.0	0.0	0.0	14.681159386251489	0.0
14	0.0	0.0	0.0	14.976149486703134	0.0
15	0.0	0.0	0.0	15.451515699532854	0.0
16	0.0	0.0	0.0	16.312529798462574	0.0
17	0.0	0.0	0.0	17.497892089051312	0.0
18	0.0	0.0	0.0	18.69852058706788	0.0
19	0.0	0.0	0.0	19.55084469100022	0.0
20	0.0	0.0	0.0	20.32472397522647	0.0
21	0.0	0.0	0.0	21.222611836712645	0.0
22	0.0	0.0	0.0	22.305807969899735	0.0
23	0.0	0.0	0.0	23.260767960693038	0.0
24	0.0	0.0	0.0	23.9562024252132	0.0
25	0.0	0.0	0.0	24.545243167197853	0.0
26	0.0	0.0	0.0	25.11760851337672	0.0
27	2.3486472965895293E-4	0.0	0.0	25.702891419686832	0.0
28	2.3486472965895293E-4	0.0	0.0	26.282772437214785	0.0
29	2.3486472965895293E-4	0.0	0.0	26.906103429729647	0.0
30	2.3486472965895293E-4	0.0	0.0	27.586036822092314	0.0
31	4.6972945931790587E-4	0.0	0.0	28.22205051000876	0.0
32	4.6972945931790587E-4	0.0	0.0	28.81015179307478	0.0
33	4.6972945931790587E-4	0.0	0.0	29.384630921820577	0.0
34	7.045941889768588E-4	0.0	0.0	29.965921127726485	0.0
35	7.045941889768588E-4	0.0	0.0	30.562712405789885	0.0
36	7.045941889768588E-4	0.0	0.0	31.20295365884019	0.0
37	7.045941889768588E-4	0.0	0.0	31.848596800672652	0.0
38	7.045941889768588E-4	0.0	0.0	32.409453775098235	0.0
39	9.394589186358117E-4	0.0	0.0	33.02996639085718	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	30	2.162269E-6	45.000004	1
TGACGAA	30	2.162269E-6	45.000004	28
CTACGAA	30	2.162269E-6	45.000004	11
GACGGCG	30	2.162269E-6	45.000004	9
CGAACGG	55	1.8189894E-12	45.0	2
GATAACG	25	3.8866543E-5	45.0	1
CTTCCGG	25	3.8866543E-5	45.0	2
GCGAACG	55	1.8189894E-12	45.0	1
GTTTACG	20	7.028279E-4	45.0	1
TAGAACG	20	7.028279E-4	45.0	1
ACGGGTC	20	7.028279E-4	45.0	5
CGTTAGT	20	7.028279E-4	45.0	43
ATTCCGC	25	3.8866543E-5	45.0	41
TCGTGCG	20	7.028279E-4	45.0	1
TCTACGG	25	3.8866543E-5	45.0	2
CGGATAT	20	7.028279E-4	45.0	6
GTCGTAG	20	7.028279E-4	45.0	1
TAGGACG	20	7.028279E-4	45.0	1
ATACGAG	45	3.8380676E-10	45.0	1
TTCGCGG	25	3.8866543E-5	45.0	2
>>END_MODULE